Click here to switch to the map view.

The map label for this gene is rfbE [H]

Identifier: 116329044

GI number: 116329044

Start: 2901526

End: 2902563

Strand: Direct

Name: rfbE [H]

Synonym: LBL_2450

Alternate gene names: 116329044

Gene position: 2901526-2902563 (Clockwise)

Preceding gene: 116329043

Following gene: 116329045

Centisome position: 80.28

GC content: 44.99

Gene sequence:

>1038_bases
ATGGCAAAAAAAGTTTTAGTGACGGGCGGGTGCGGATTTCTGGGCTCCCACGTTTGTGAAACGTTTCGTAAACAAGGTTG
GGACGTTGTCAGCTACGACAGTATGACCAAATACGAGTTGAAAAGAACCGGATACGGTACCGAAGCTACTCGAGAATATA
ATTGGAATTTTTTAAAAGATCTCGGTGTTACGATGGTGAAAGGGGATATCAGAAACCTTGAGCATCTTGCGGATCGTACG
GAAGGTTGCGATTTTATCGTTCATACTGCGGCACAACCTGCGATGACGATTTCCTGGGAAGATCCCGAACTCGACTTTAC
CACAAATGCATTGGGAACTTTTAATGTTTTGGAAGTCGCCCGTAAACGGAATATTCCGGTCGTTAATACGAGTTCCATAC
ATGTATACGGAAATTCAATCAACGACACGCTTAAAGAAGGAGCCACATCCTACGAAAGAAACCCGGCAGCGATCGGAGAA
GATCAACCCGTGATGGTGGGGGAAATTTCCCCGCTTCACGCTTCTAAGATGAGTGCGGAACACTACGTAAAAACGTACGT
GGATATATATAAGTTGAAGGCAGCGACGTTTCGTTTTACGGGGATTTACGGCGAACGCCAGTTTGGCGGAGAAGATCATG
GCTGGGTGGCGAACTTTGCGATTCGTTCCGTGTTCGATTGGCCTTTGCGGATTTTCGGAACTGGTAAACAAGCACGCGAT
ATCCTTTATGCCGTGGACGGAGCTGAGAGTTATCTCAGATGGTTCGAGAATCCAACTCCGGGAGTTTTTAACATCGGCGG
CGGTCCCGAACACAAGATTTCTTTATTAGAATGTATTCATCTGATCGGAGATATACTCGGTAAAAAACAGGAAATCGTAT
TTGACGTAGAAAGACCGGGAGATATGCGTTATTTTATCTGCGATATTACCAAGGCGAAAAAATTCGGATTCAACCCTAAA
TTCAAACCGAGAGAAGGTGTGGAAAGATTGATTCACTGGATCCAAGCGGATAAATCCGTATTCGAAGTCAAAAAATGA

Upstream 100 bases:

>100_bases
CCCGAGAGTTCCTTTCAATCGTTCGTTTGATGCTGAAGAAAAAATGGCTCGGAATCTAATTTAGAATTTTATAATTATAC
AATATTAAAAAGGGATATTT

Downstream 100 bases:

>100_bases
AAAATCTCGTCGTCATTCCCGCTTACAACGAGGAGAAAACGATTCGCGAAGTTGTCGAACGGGCGTTGACGTATTCGGAC
GTTCTCGTTGTCGACGACGC

Product: nucleoside-diphosphate-sugar epimerase

Products: NA

Alternate protein names: CDP-tyvelose 2-epimerase [H]

Number of amino acids: Translated: 345; Mature: 344

Protein sequence:

>345_residues
MAKKVLVTGGCGFLGSHVCETFRKQGWDVVSYDSMTKYELKRTGYGTEATREYNWNFLKDLGVTMVKGDIRNLEHLADRT
EGCDFIVHTAAQPAMTISWEDPELDFTTNALGTFNVLEVARKRNIPVVNTSSIHVYGNSINDTLKEGATSYERNPAAIGE
DQPVMVGEISPLHASKMSAEHYVKTYVDIYKLKAATFRFTGIYGERQFGGEDHGWVANFAIRSVFDWPLRIFGTGKQARD
ILYAVDGAESYLRWFENPTPGVFNIGGGPEHKISLLECIHLIGDILGKKQEIVFDVERPGDMRYFICDITKAKKFGFNPK
FKPREGVERLIHWIQADKSVFEVKK

Sequences:

>Translated_345_residues
MAKKVLVTGGCGFLGSHVCETFRKQGWDVVSYDSMTKYELKRTGYGTEATREYNWNFLKDLGVTMVKGDIRNLEHLADRT
EGCDFIVHTAAQPAMTISWEDPELDFTTNALGTFNVLEVARKRNIPVVNTSSIHVYGNSINDTLKEGATSYERNPAAIGE
DQPVMVGEISPLHASKMSAEHYVKTYVDIYKLKAATFRFTGIYGERQFGGEDHGWVANFAIRSVFDWPLRIFGTGKQARD
ILYAVDGAESYLRWFENPTPGVFNIGGGPEHKISLLECIHLIGDILGKKQEIVFDVERPGDMRYFICDITKAKKFGFNPK
FKPREGVERLIHWIQADKSVFEVKK
>Mature_344_residues
AKKVLVTGGCGFLGSHVCETFRKQGWDVVSYDSMTKYELKRTGYGTEATREYNWNFLKDLGVTMVKGDIRNLEHLADRTE
GCDFIVHTAAQPAMTISWEDPELDFTTNALGTFNVLEVARKRNIPVVNTSSIHVYGNSINDTLKEGATSYERNPAAIGED
QPVMVGEISPLHASKMSAEHYVKTYVDIYKLKAATFRFTGIYGERQFGGEDHGWVANFAIRSVFDWPLRIFGTGKQARDI
LYAVDGAESYLRWFENPTPGVFNIGGGPEHKISLLECIHLIGDILGKKQEIVFDVERPGDMRYFICDITKAKKFGFNPKF
KPREGVERLIHWIQADKSVFEVKK

Specific function: Catalyzes the isomeration of CDP-paratose to CDP- tyvelose [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=350, Percent_Identity=26.5714285714286, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI42516563, Length=341, Percent_Identity=24.633431085044, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI48994969, Length=370, Percent_Identity=25.4054054054054, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1788353, Length=361, Percent_Identity=24.9307479224377, Blast_Score=75, Evalue=5e-15,
Organism=Drosophila melanogaster, GI21356223, Length=339, Percent_Identity=24.188790560472, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =5.1.3.10 [H]

Molecular weight: Translated: 38971; Mature: 38840

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKVLVTGGCGFLGSHVCETFRKQGWDVVSYDSMTKYELKRTGYGTEATREYNWNFLKD
CCCEEEEECCCHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHCCCCCCCCEECCCHHHHH
LGVTMVKGDIRNLEHLADRTEGCDFIVHTAAQPAMTISWEDPELDFTTNALGTFNVLEVA
CCCEEEHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCCCCEECCCCCHHHHHHHH
RKRNIPVVNTSSIHVYGNSINDTLKEGATSYERNPAAIGEDQPVMVGEISPLHASKMSAE
HHCCCCEEECCEEEEECCCHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHH
HYVKTYVDIYKLKAATFRFTGIYGERQFGGEDHGWVANFAIRSVFDWPLRIFGTGKQARD
HHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCHHHCE
ILYAVDGAESYLRWFENPTPGVFNIGGGPEHKISLLECIHLIGDILGKKQEIVFDVERPG
EEEEECCHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCC
DMRYFICDITKAKKFGFNPKFKPREGVERLIHWIQADKSVFEVKK
CCEEEEEEECHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHCCC
>Mature Secondary Structure 
AKKVLVTGGCGFLGSHVCETFRKQGWDVVSYDSMTKYELKRTGYGTEATREYNWNFLKD
CCEEEEECCCHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHCCCCCCCCEECCCHHHHH
LGVTMVKGDIRNLEHLADRTEGCDFIVHTAAQPAMTISWEDPELDFTTNALGTFNVLEVA
CCCEEEHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCCCCEECCCCCHHHHHHHH
RKRNIPVVNTSSIHVYGNSINDTLKEGATSYERNPAAIGEDQPVMVGEISPLHASKMSAE
HHCCCCEEECCEEEEECCCHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHH
HYVKTYVDIYKLKAATFRFTGIYGERQFGGEDHGWVANFAIRSVFDWPLRIFGTGKQARD
HHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCHHHCE
ILYAVDGAESYLRWFENPTPGVFNIGGGPEHKISLLECIHLIGDILGKKQEIVFDVERPG
EEEEECCHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCC
DMRYFICDITKAKKFGFNPKFKPREGVERLIHWIQADKSVFEVKK
CCEEEEEEECHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2793833; 11677608; 12644504; 12642575 [H]