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The map label for this gene is pleD [H]

Identifier: 116328980

GI number: 116328980

Start: 2824681

End: 2825811

Strand: Reverse

Name: pleD [H]

Synonym: LBL_2382

Alternate gene names: 116328980

Gene position: 2825811-2824681 (Counterclockwise)

Preceding gene: 116328981

Following gene: 116328979

Centisome position: 78.18

GC content: 43.15

Gene sequence:

>1131_bases
TTGTCCTCTCAAGATTATAGTCTTTATACGGTTCTTGCGGTCGACGATTCCGAAATAAATCTAAAGTTGATTGTACATAC
CCTCAAACCTTTGGGCTTTCAGATTTTTACGGCGGAGTCCGCCGCCGATGCGCGTAGTGTTCTTCTTACGAATCGAGTGG
ACGTACTTTTGTTGGATGTAAGTATGCCCGATCAAGACGGTTTTTCCTTTTGTAGAGAACTTAGAGAAATCGAAAGGTTC
AAACTTCTTCCCATTCTTTTTATCACCGGGATCAATCGTGAAGTCGGTTTTGACGAGGCGATTTCTCACGGTGGAGACGA
CTTCATCCAAAAACCGTTTCAGCCGAAAGAGTTGATCGCCAAAATCCGCGCTTTCATTCGGATTAAAATTCTTCAAGACG
AGGTTTTAGAACAAAAAAGAAATTACGAGAGGGAACTCATCATGGCGAGAAAGGTCCAGCAGGAACTTCTTCCTGAAAAA
GAATTGGAATGGAACGGAGTCAGTGTAAGTACGATCTTTCAACCTTTAATGCAAATCGGCGGGGATTACACGGACGCTTG
GATTGAAAACGATTCTCTCCATATTTTTATCGCGGATTGTTCCGGCCACGGTCCTTCCGCGGCCTTGCTCGCTGCGATGC
TTAAGATGCAGGTTTCCAGTCTTTCCCCGAACCAAAGTTTGCAAGAGAAGGTGCAGACGCTTCGCCATAATCTTGAGAAA
ATTCTTCCCGACGAATTTTCGATTACGTTCTTTTACGGAATCCTTCGCAAAGATTTGAACTTCGAGTATTCGAACGGAGG
ACATCCTCCTCCGATCCTCTATCAAAATGGAGAGGTTACGATTCTTTCCGGAATGGGACCTCTCATCATTCCGATCGAAA
TCAACGTAAAAGAAGAATTCAAATCCGTTCGGTTTGAGAAAGGTTCTTATCTTCTTCTTTATACGGACGGTGCAACCGAA
ATCACAGACAAGAGTATGAACATTCTCGGAGAAGAAAAATTGAAAAAGATCTTTCAAGAAAGTGTTTCCAAGGAAGGAGA
TATTCTCGTTTCTATGATGGAAGCCATTCTTGCACATTCGGACAGAGGCACGAATGATGACGATATCGCTATGATGGTAT
TGAAACTATGA

Upstream 100 bases:

>100_bases
GTATCATATTCTATTTTTGATGTTGTTCCGTTTCGCTTATCGTTTGTCTAAGATCGAAGATCTTCGTGAGAATTTACCGG
AAAGAGAAGAGTAAAAAACC

Downstream 100 bases:

>100_bases
ATTTACCGACCTACAGAGGAAAAGTTAGGGACATCTATGATCTCGGTGATAGACTGATTCTTTCTTCTTCGGATCGTATT
TCCGCTTTCGACGTCGTATT

Product: Serine phosphatase, regulator of sigma subunit

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MSSQDYSLYTVLAVDDSEINLKLIVHTLKPLGFQIFTAESAADARSVLLTNRVDVLLLDVSMPDQDGFSFCRELREIERF
KLLPILFITGINREVGFDEAISHGGDDFIQKPFQPKELIAKIRAFIRIKILQDEVLEQKRNYERELIMARKVQQELLPEK
ELEWNGVSVSTIFQPLMQIGGDYTDAWIENDSLHIFIADCSGHGPSAALLAAMLKMQVSSLSPNQSLQEKVQTLRHNLEK
ILPDEFSITFFYGILRKDLNFEYSNGGHPPPILYQNGEVTILSGMGPLIIPIEINVKEEFKSVRFEKGSYLLLYTDGATE
ITDKSMNILGEEKLKKIFQESVSKEGDILVSMMEAILAHSDRGTNDDDIAMMVLKL

Sequences:

>Translated_376_residues
MSSQDYSLYTVLAVDDSEINLKLIVHTLKPLGFQIFTAESAADARSVLLTNRVDVLLLDVSMPDQDGFSFCRELREIERF
KLLPILFITGINREVGFDEAISHGGDDFIQKPFQPKELIAKIRAFIRIKILQDEVLEQKRNYERELIMARKVQQELLPEK
ELEWNGVSVSTIFQPLMQIGGDYTDAWIENDSLHIFIADCSGHGPSAALLAAMLKMQVSSLSPNQSLQEKVQTLRHNLEK
ILPDEFSITFFYGILRKDLNFEYSNGGHPPPILYQNGEVTILSGMGPLIIPIEINVKEEFKSVRFEKGSYLLLYTDGATE
ITDKSMNILGEEKLKKIFQESVSKEGDILVSMMEAILAHSDRGTNDDDIAMMVLKL
>Mature_375_residues
SSQDYSLYTVLAVDDSEINLKLIVHTLKPLGFQIFTAESAADARSVLLTNRVDVLLLDVSMPDQDGFSFCRELREIERFK
LLPILFITGINREVGFDEAISHGGDDFIQKPFQPKELIAKIRAFIRIKILQDEVLEQKRNYERELIMARKVQQELLPEKE
LEWNGVSVSTIFQPLMQIGGDYTDAWIENDSLHIFIADCSGHGPSAALLAAMLKMQVSSLSPNQSLQEKVQTLRHNLEKI
LPDEFSITFFYGILRKDLNFEYSNGGHPPPILYQNGEVTILSGMGPLIIPIEINVKEEFKSVRFEKGSYLLLYTDGATEI
TDKSMNILGEEKLKKIFQESVSKEGDILVSMMEAILAHSDRGTNDDDIAMMVLKL

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2208

COG function: function code TK; Serine phosphatase RsbU, regulator of sigma subunit

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1789809, Length=119, Percent_Identity=35.2941176470588, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1790860, Length=118, Percent_Identity=33.0508474576271, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790863, Length=133, Percent_Identity=27.8195488721804, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786599, Length=117, Percent_Identity=31.6239316239316, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1788394, Length=95, Percent_Identity=34.7368421052632, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1786911, Length=120, Percent_Identity=29.1666666666667, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI145693157, Length=102, Percent_Identity=33.3333333333333, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787375, Length=119, Percent_Identity=33.6134453781513, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 42537; Mature: 42405

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSQDYSLYTVLAVDDSEINLKLIVHTLKPLGFQIFTAESAADARSVLLTNRVDVLLLDV
CCCCCCEEEEEEEECCCCCEEEEEEEHHCCCCEEEEECCCCCCHHHHHHCCCCEEEEEEE
SMPDQDGFSFCRELREIERFKLLPILFITGINREVGFDEAISHGGDDFIQKPFQPKELIA
CCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCHHHHHCCCCHHHHCCCCHHHHHH
KIRAFIRIKILQDEVLEQKRNYERELIMARKVQQELLPEKELEWNGVSVSTIFQPLMQIG
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHC
GDYTDAWIENDSLHIFIADCSGHGPSAALLAAMLKMQVSSLSPNQSLQEKVQTLRHNLEK
CCCCHHEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
ILPDEFSITFFYGILRKDLNFEYSNGGHPPPILYQNGEVTILSGMGPLIIPIEINVKEEF
HCCCCCHHHHHHHHHHHHCCCEECCCCCCCCEEEECCCEEEEECCCCEEEEEEECCHHHH
KSVRFEKGSYLLLYTDGATEITDKSMNILGEEKLKKIFQESVSKEGDILVSMMEAILAHS
HHHCCCCCCEEEEEECCCCHHCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
DRGTNDDDIAMMVLKL
CCCCCCCCEEEEEEEC
>Mature Secondary Structure 
SSQDYSLYTVLAVDDSEINLKLIVHTLKPLGFQIFTAESAADARSVLLTNRVDVLLLDV
CCCCCEEEEEEEECCCCCEEEEEEEHHCCCCEEEEECCCCCCHHHHHHCCCCEEEEEEE
SMPDQDGFSFCRELREIERFKLLPILFITGINREVGFDEAISHGGDDFIQKPFQPKELIA
CCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCHHHHHCCCCHHHHCCCCHHHHHH
KIRAFIRIKILQDEVLEQKRNYERELIMARKVQQELLPEKELEWNGVSVSTIFQPLMQIG
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHC
GDYTDAWIENDSLHIFIADCSGHGPSAALLAAMLKMQVSSLSPNQSLQEKVQTLRHNLEK
CCCCHHEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
ILPDEFSITFFYGILRKDLNFEYSNGGHPPPILYQNGEVTILSGMGPLIIPIEINVKEEF
HCCCCCHHHHHHHHHHHHCCCEECCCCCCCCEEEECCCEEEEECCCCEEEEEEECCHHHH
KSVRFEKGSYLLLYTDGATEITDKSMNILGEEKLKKIFQESVSKEGDILVSMMEAILAHS
HHHCCCCCCEEEEEECCCCHHCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
DRGTNDDDIAMMVLKL
CCCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA