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The map label for this gene is pyrB

Identifier: 116328948

GI number: 116328948

Start: 2783365

End: 2784291

Strand: Direct

Name: pyrB

Synonym: LBL_2345

Alternate gene names: 116328948

Gene position: 2783365-2784291 (Clockwise)

Preceding gene: 116328946

Following gene: 116328949

Centisome position: 77.01

GC content: 46.17

Gene sequence:

>927_bases
ATGTCCTATAACCACAAAAATGTCCTAGATACGGAACAGTTTTCTAAATCGGATCTCGACTTCCTCATCAAGAAAATCAA
GGATATGGAGCACCTCGTCGAGCGGCACAAGGCGTTCGGAATCTTAACCGGAAAACTTTTAGCCTCCCTCTTTTTCGAGG
CTTCGACAAGAACTAGACTTTCTTTCGAAGCGGCGATGGAACGCCTCGGAGGTAGAGTGATCTCCACAGTCGGTTTTCAG
TTTTCCTCGATTTCCAAAGGAGAAACGTTATATGACACCATGAAAATGGTAGAAGCTTACGCGGACATCGCGGTGATTCG
TCATCCCGTCGAAGGCTCTTCTAGAATCGCGGCTGGAGCGGTAAAAATTCCGGTCGTCAACGCGGGGGACGGAGCAGGAC
AACACCCTACTCAGGCGATTCTGGATCTTTATACGATCATTTCCGAAAAAGGAACGTTAGACGGATTGTCGGTCGCTTTT
ATCGGAGATTTGAAGTATGGAAGAACGATTCATTCGCTTATCAACCTTCTCAGGCACTACAAGGTTCGTCTGTATCTGAT
TTCTCCTATAGAACTCGCCTTGCCCGATTCGTACAAAAAAGGTCTGGAAGGTTACCCTCTCACTCTGGAAGAAACGACCG
ACATCAAAGCCGTCTGGGAATGCGACGTCGCTTATGTAACTCGAATTCAGGAAGAAAGATTTCCAGATCACAAGGAATAC
GAAAGGCTCAAGGATCTTTTTAAAATCAACAAGGAGTTGATTCTTGCTTCCAAGAAAGAGACGACGATTCTTCACCCTCT
TCCGAGAGTGAACGAACTTTCCACGGACGTGGATGATTTGCCGAATGCGGCATATTTCAGACAAGCAAGATACGGAGTCG
TAAGTCGTATGACCCTACTTTGTCTTTGTTTAGGACAGGACTTCTGA

Upstream 100 bases:

>100_bases
TGCTCCGGATGGGGCGCCATATCAACTCTCCCTGAAAAATCCGAAAACTCATAAATCAATTTGATTGTCAAAAGGGCGTT
TTTCTACATTCTGTCGCTTA

Downstream 100 bases:

>100_bases
ATATTTTTGGAACGGAAACTCTGGACATACGAAGAGGCCCGTAAAATTCTACCGGTAATCAGGGAGATTACGGAAGAATA
CTATTCTTATATATCGGAAC

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MSYNHKNVLDTEQFSKSDLDFLIKKIKDMEHLVERHKAFGILTGKLLASLFFEASTRTRLSFEAAMERLGGRVISTVGFQ
FSSISKGETLYDTMKMVEAYADIAVIRHPVEGSSRIAAGAVKIPVVNAGDGAGQHPTQAILDLYTIISEKGTLDGLSVAF
IGDLKYGRTIHSLINLLRHYKVRLYLISPIELALPDSYKKGLEGYPLTLEETTDIKAVWECDVAYVTRIQEERFPDHKEY
ERLKDLFKINKELILASKKETTILHPLPRVNELSTDVDDLPNAAYFRQARYGVVSRMTLLCLCLGQDF

Sequences:

>Translated_308_residues
MSYNHKNVLDTEQFSKSDLDFLIKKIKDMEHLVERHKAFGILTGKLLASLFFEASTRTRLSFEAAMERLGGRVISTVGFQ
FSSISKGETLYDTMKMVEAYADIAVIRHPVEGSSRIAAGAVKIPVVNAGDGAGQHPTQAILDLYTIISEKGTLDGLSVAF
IGDLKYGRTIHSLINLLRHYKVRLYLISPIELALPDSYKKGLEGYPLTLEETTDIKAVWECDVAYVTRIQEERFPDHKEY
ERLKDLFKINKELILASKKETTILHPLPRVNELSTDVDDLPNAAYFRQARYGVVSRMTLLCLCLGQDF
>Mature_307_residues
SYNHKNVLDTEQFSKSDLDFLIKKIKDMEHLVERHKAFGILTGKLLASLFFEASTRTRLSFEAAMERLGGRVISTVGFQF
SSISKGETLYDTMKMVEAYADIAVIRHPVEGSSRIAAGAVKIPVVNAGDGAGQHPTQAILDLYTIISEKGTLDGLSVAFI
GDLKYGRTIHSLINLLRHYKVRLYLISPIELALPDSYKKGLEGYPLTLEETTDIKAVWECDVAYVTRIQEERFPDHKEYE
RLKDLFKINKELILASKKETTILHPLPRVNELSTDVDDLPNAAYFRQARYGVVSRMTLLCLCLGQDF

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=302, Percent_Identity=43.046357615894, Blast_Score=262, Evalue=3e-70,
Organism=Escherichia coli, GI2367364, Length=311, Percent_Identity=42.1221864951768, Blast_Score=233, Evalue=9e-63,
Organism=Caenorhabditis elegans, GI193204318, Length=320, Percent_Identity=41.25, Blast_Score=218, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6322331, Length=308, Percent_Identity=43.8311688311688, Blast_Score=270, Evalue=2e-73,
Organism=Saccharomyces cerevisiae, GI6322373, Length=278, Percent_Identity=27.3381294964029, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24642586, Length=303, Percent_Identity=43.8943894389439, Blast_Score=249, Evalue=2e-66,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_LEPBJ (Q04UL7)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_800161.1
- ProteinModelPortal:   Q04UL7
- SMR:   Q04UL7
- STRING:   Q04UL7
- GeneID:   4409628
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_0731
- eggNOG:   COG0540
- HOGENOM:   HBG579429
- OMA:   FKQSFYG
- PhylomeDB:   Q04UL7
- ProtClustDB:   PRK00856
- BioCyc:   LBOR355277:LBJ_0731-MONOMER
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 34723; Mature: 34591

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYNHKNVLDTEQFSKSDLDFLIKKIKDMEHLVERHKAFGILTGKLLASLFFEASTRTRL
CCCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
SFEAAMERLGGRVISTVGFQFSSISKGETLYDTMKMVEAYADIAVIRHPVEGSSRIAAGA
HHHHHHHHHCCHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECE
VKIPVVNAGDGAGQHPTQAILDLYTIISEKGTLDGLSVAFIGDLKYGRTIHSLINLLRHY
EEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHH
KVRLYLISPIELALPDSYKKGLEGYPLTLEETTDIKAVWECDVAYVTRIQEERFPDHKEY
EEEEEEECCHHEECCCHHHCCCCCCCEEEECCCCCEEHHCCHHHHHHHHHHHHCCCHHHH
ERLKDLFKINKELILASKKETTILHPLPRVNELSTDVDDLPNAAYFRQARYGVVSRMTLL
HHHHHHHHHCHHHEEECCCCCEEECCCCCCHHHCCCHHHCCCHHHHHHHHHHHHHHHHHH
CLCLGQDF
HHHHCCCC
>Mature Secondary Structure 
SYNHKNVLDTEQFSKSDLDFLIKKIKDMEHLVERHKAFGILTGKLLASLFFEASTRTRL
CCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
SFEAAMERLGGRVISTVGFQFSSISKGETLYDTMKMVEAYADIAVIRHPVEGSSRIAAGA
HHHHHHHHHCCHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECE
VKIPVVNAGDGAGQHPTQAILDLYTIISEKGTLDGLSVAFIGDLKYGRTIHSLINLLRHY
EEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHH
KVRLYLISPIELALPDSYKKGLEGYPLTLEETTDIKAVWECDVAYVTRIQEERFPDHKEY
EEEEEEECCHHEECCCHHHCCCCCCCEEEECCCCCEEHHCCHHHHHHHHHHHHCCCHHHH
ERLKDLFKINKELILASKKETTILHPLPRVNELSTDVDDLPNAAYFRQARYGVVSRMTLL
HHHHHHHHHCHHHEEECCCCCEEECCCCCCHHHCCCHHHCCCHHHHHHHHHHHHHHHHHH
CLCLGQDF
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA