| Definition | Leptospira borgpetersenii serovar Hardjo-bovis L550 chromosome 1, complete sequence. |
|---|---|
| Accession | NC_008508 |
| Length | 3,614,446 |
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The map label for this gene is gcpE [H]
Identifier: 116328946
GI number: 116328946
Start: 2777820
End: 2779811
Strand: Direct
Name: gcpE [H]
Synonym: LBL_2341
Alternate gene names: 116328946
Gene position: 2777820-2779811 (Clockwise)
Preceding gene: 116328944
Following gene: 116328948
Centisome position: 76.85
GC content: 43.72
Gene sequence:
>1992_bases ATGAATTTTAGATACAATCACACACCCTTCGGTTATAAAAGAAGACAAACCAGAGAAGTTAAAGTCGGAGACGTCAAAAT TGGGGGAAATAATCCGATCGTCATTCAATCGATGATCAATAGTGATACGACCGACACGAAAGGGACAGTAAAACAAATTC TCGAGTTAGAACGCACGGGTTGCGAAATTGTAAGATTTACAGTTCCGTCACAAGTGGATGCGGACAATCTTCCTTCGATC CGACAAGAACTTAAAAAGGCGGGAAGCAAAATTCCCCTCGTTGCTGATATTCATTTTACTCCTAGCGTCGCAATGAAAGC GGTCGAGTATGTGGAAAAGGTGAGAATCAATCCTGGTAATTTTGCGGATAAGAAAAAATTCGCAGTGAGAGATTATACGG ATTCGGAGTACGACGAAGAATTGGAAAGGATTTCGGCGATATTTTCCCCTCTCGTTCTGCGTTGCAAGGAACTCGGAGTC TCAATGAGAATCGGTACTAATCACGGTTCTCTTTCCGATCGAATTATGAATCGGTACGGAGACACTCCGCAAGGAATGGT GGAGTCCGCACTCGAATTTATCCGGATTGCTGAAAGTTTAAATTATTATGATATTGTTGTAAGCATGAAAGCCTCAAATC CGCAGGTGATGGTTCAGGCATACAGAATGCTCGCTTCAAGGTTTAACGAACTGAAAATGGATTACCCTCTTCACTTGGGA GTCACCGAAGCGGGGGACGGTAAGGACGGCAGAATCAAATCCGCCATCGGAATCGGTTCCCTCTTGGAAGACGGACTCGG AGATACAATTCGAGTTTCCCTCACCGAAGACCCAGTTTTGGAAATTCCCGTCGCAAGACTCCTCGCGGAGAAATTCAACA AAAGAATCGTAAAACCCGAACCGGTAAGAGGTTACTCTGAATTTAGAAATCCATTTACTTATGAAAGATTTTACAGCAGC GAAATCAAAGTCGGAACGTTCGAAGCCGGAGAAAATCATCCTGTTCGGGTGGAGACCGTTTTGCCTTTCGAGAATTCCAA CTCATTCTTAGCAAACATCGCAAAACTCTATCAGTACGGAAAATCTTTTTCGATCGAACCGGAAAGTATCTTAATAGATT CCCCTTCTCCCGATCAGTTGAAAGAAATTTCCGAAGCCGCGGCCGCGCTTTCAATCCCCGTGGGAATACTTCTCGGAAAG AATGTCTCTTTAAACGAAAAACTCCAAAATGAATTAAGGGGATTCCCGAAAGTCGTGTTTGATCCGTTTCTTCAGTTTCA AGACGGAAAAAAAATGTTATCCTTTTTGCAAGAAAGACAAAACGCGGGACTTTATACGGAAATCCATACGAGCGGAGCAA AAATAGAATCCTTCAAGGGCCTTCCGGAAACGTTATCTGAAATTGGAATCAAAAACGTCCTGTTTTCGATCGAATCGAAA GAAATCCTTTACGATTACAGAAAACTCGGAAGCATTCTGAGCCAACACGAGTTCCCGATCTTACTTCACGGTTCTTTCTC AAATCCGGAAGAAGCTTTGTACGATTCCGCAATCGGTATCGGAGGTCTTCTGATCGACGGAATTGGCGATCTAATCCGAA TCAAAACTCCCAAGATGAAAGATATCGAGGAAATCTTCCAACTTTCTTATGATCTCTTGCAGGGAACCAGACTTCGTTTA ACAAAAACGGAATATATTTCCTGTCCTTCCTGCGGACGGACTCTCTTCAACCTCCAAGAAACAACCGCAAGAATCAAGTC CAGAACCGGACATCTCAAAGGCGTAAAAATCGCCGTGATGGGTTGTATCGTAAACGGCCCTGGTGAAATGGCGGACGCGG ATTTCGGTTACGTAGGTGCAGGCCCGGGCAAAGTACATCTCTATCGTGGAAAAGAAATCGTTATGAAAAACGTTCCGAGC GAAGTTGCGGACGAAAAACTCGTGGAACTCATAAAAAAACATGGACTGTGGCAGGATGTTATAAACGTTTAA
Upstream 100 bases:
>100_bases GAAAGAAAGTCGTAAAAAAGGAGAGGACTGGACGAGAACGGCCTACAAAATTGCCTTAGGTAAAGAGGAAACCGGCTTAT AATCGGAATTCTTAACTAGA
Downstream 100 bases:
>100_bases GGTAATTTGCACACGATTTTTTGATCGAATAGATTCAAGAAAGTAAAGATTAGAATAACGGAAATAATCCTAAAAACAGA GAATTAAACCGATTGTCTTG
Product: deoxyxylulose biosynthesis protein
Products: NA
Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]
Number of amino acids: Translated: 663; Mature: 663
Protein sequence:
>663_residues MNFRYNHTPFGYKRRQTREVKVGDVKIGGNNPIVIQSMINSDTTDTKGTVKQILELERTGCEIVRFTVPSQVDADNLPSI RQELKKAGSKIPLVADIHFTPSVAMKAVEYVEKVRINPGNFADKKKFAVRDYTDSEYDEELERISAIFSPLVLRCKELGV SMRIGTNHGSLSDRIMNRYGDTPQGMVESALEFIRIAESLNYYDIVVSMKASNPQVMVQAYRMLASRFNELKMDYPLHLG VTEAGDGKDGRIKSAIGIGSLLEDGLGDTIRVSLTEDPVLEIPVARLLAEKFNKRIVKPEPVRGYSEFRNPFTYERFYSS EIKVGTFEAGENHPVRVETVLPFENSNSFLANIAKLYQYGKSFSIEPESILIDSPSPDQLKEISEAAAALSIPVGILLGK NVSLNEKLQNELRGFPKVVFDPFLQFQDGKKMLSFLQERQNAGLYTEIHTSGAKIESFKGLPETLSEIGIKNVLFSIESK EILYDYRKLGSILSQHEFPILLHGSFSNPEEALYDSAIGIGGLLIDGIGDLIRIKTPKMKDIEEIFQLSYDLLQGTRLRL TKTEYISCPSCGRTLFNLQETTARIKSRTGHLKGVKIAVMGCIVNGPGEMADADFGYVGAGPGKVHLYRGKEIVMKNVPS EVADEKLVELIKKHGLWQDVINV
Sequences:
>Translated_663_residues MNFRYNHTPFGYKRRQTREVKVGDVKIGGNNPIVIQSMINSDTTDTKGTVKQILELERTGCEIVRFTVPSQVDADNLPSI RQELKKAGSKIPLVADIHFTPSVAMKAVEYVEKVRINPGNFADKKKFAVRDYTDSEYDEELERISAIFSPLVLRCKELGV SMRIGTNHGSLSDRIMNRYGDTPQGMVESALEFIRIAESLNYYDIVVSMKASNPQVMVQAYRMLASRFNELKMDYPLHLG VTEAGDGKDGRIKSAIGIGSLLEDGLGDTIRVSLTEDPVLEIPVARLLAEKFNKRIVKPEPVRGYSEFRNPFTYERFYSS EIKVGTFEAGENHPVRVETVLPFENSNSFLANIAKLYQYGKSFSIEPESILIDSPSPDQLKEISEAAAALSIPVGILLGK NVSLNEKLQNELRGFPKVVFDPFLQFQDGKKMLSFLQERQNAGLYTEIHTSGAKIESFKGLPETLSEIGIKNVLFSIESK EILYDYRKLGSILSQHEFPILLHGSFSNPEEALYDSAIGIGGLLIDGIGDLIRIKTPKMKDIEEIFQLSYDLLQGTRLRL TKTEYISCPSCGRTLFNLQETTARIKSRTGHLKGVKIAVMGCIVNGPGEMADADFGYVGAGPGKVHLYRGKEIVMKNVPS EVADEKLVELIKKHGLWQDVINV >Mature_663_residues MNFRYNHTPFGYKRRQTREVKVGDVKIGGNNPIVIQSMINSDTTDTKGTVKQILELERTGCEIVRFTVPSQVDADNLPSI RQELKKAGSKIPLVADIHFTPSVAMKAVEYVEKVRINPGNFADKKKFAVRDYTDSEYDEELERISAIFSPLVLRCKELGV SMRIGTNHGSLSDRIMNRYGDTPQGMVESALEFIRIAESLNYYDIVVSMKASNPQVMVQAYRMLASRFNELKMDYPLHLG VTEAGDGKDGRIKSAIGIGSLLEDGLGDTIRVSLTEDPVLEIPVARLLAEKFNKRIVKPEPVRGYSEFRNPFTYERFYSS EIKVGTFEAGENHPVRVETVLPFENSNSFLANIAKLYQYGKSFSIEPESILIDSPSPDQLKEISEAAAALSIPVGILLGK NVSLNEKLQNELRGFPKVVFDPFLQFQDGKKMLSFLQERQNAGLYTEIHTSGAKIESFKGLPETLSEIGIKNVLFSIESK EILYDYRKLGSILSQHEFPILLHGSFSNPEEALYDSAIGIGGLLIDGIGDLIRIKTPKMKDIEEIFQLSYDLLQGTRLRL TKTEYISCPSCGRTLFNLQETTARIKSRTGHLKGVKIAVMGCIVNGPGEMADADFGYVGAGPGKVHLYRGKEIVMKNVPS EVADEKLVELIKKHGLWQDVINV
Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]
COG id: COG0821
COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the ispG family [H]
Homologues:
Organism=Escherichia coli, GI1788863, Length=283, Percent_Identity=41.696113074205, Blast_Score=199, Evalue=3e-52,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR017178 - InterPro: IPR004588 [H]
Pfam domain/function: PF04551 GcpE [H]
EC number: =1.17.7.1 [H]
Molecular weight: Translated: 74012; Mature: 74012
Theoretical pI: Translated: 6.75; Mature: 6.75
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.8 %Cys (Translated Protein) 2.3 %Met (Translated Protein) 3.0 %Cys+Met (Translated Protein) 0.8 %Cys (Mature Protein) 2.3 %Met (Mature Protein) 3.0 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MNFRYNHTPFGYKRRQTREVKVGDVKIGGNNPIVIQSMINSDTTDTKGTVKQILELERTG CCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCC CEIVRFTVPSQVDADNLPSIRQELKKAGSKIPLVADIHFTPSVAMKAVEYVEKVRINPGN CEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCC FADKKKFAVRDYTDSEYDEELERISAIFSPLVLRCKELGVSMRIGTNHGSLSDRIMNRYG CCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHCC DTPQGMVESALEFIRIAESLNYYDIVVSMKASNPQVMVQAYRMLASRFNELKMDYPLHLG CCHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCEEEC VTEAGDGKDGRIKSAIGIGSLLEDGLGDTIRVSLTEDPVLEIPVARLLAEKFNKRIVKPE CCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCC PVRGYSEFRNPFTYERFYSSEIKVGTFEAGENHPVRVETVLPFENSNSFLANIAKLYQYG CCCCHHHHCCCCCHHHHHCCCCEEEEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHCC KSFSIEPESILIDSPSPDQLKEISEAAAALSIPVGILLGKNVSLNEKLQNELRGFPKVVF CCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHCCCCHHHH DPFLQFQDGKKMLSFLQERQNAGLYTEIHTSGAKIESFKGLPETLSEIGIKNVLFSIESK HHHHHHCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHCCCCHHHHHHHCHHHHHHCCCCH EILYDYRKLGSILSQHEFPILLHGSFSNPEEALYDSAIGIGGLLIDGIGDLIRIKTPKMK HHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHCCCHHHHHCCCCEEEECCCCHH DIEEIFQLSYDLLQGTRLRLTKTEYISCPSCGRTLFNLQETTARIKSRTGHLKGVKIAVM HHHHHHHHHHHHHCCCEEEEEECCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEE GCIVNGPGEMADADFGYVGAGPGKVHLYRGKEIVMKNVPSEVADEKLVELIKKHGLWQDV EEEECCCCCCCCCCCCEECCCCCEEEEEECCCHHHHCCCHHHHHHHHHHHHHHCCCHHHH INV HCC >Mature Secondary Structure MNFRYNHTPFGYKRRQTREVKVGDVKIGGNNPIVIQSMINSDTTDTKGTVKQILELERTG CCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCC CEIVRFTVPSQVDADNLPSIRQELKKAGSKIPLVADIHFTPSVAMKAVEYVEKVRINPGN CEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCC FADKKKFAVRDYTDSEYDEELERISAIFSPLVLRCKELGVSMRIGTNHGSLSDRIMNRYG CCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHCC DTPQGMVESALEFIRIAESLNYYDIVVSMKASNPQVMVQAYRMLASRFNELKMDYPLHLG CCHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCEEEC VTEAGDGKDGRIKSAIGIGSLLEDGLGDTIRVSLTEDPVLEIPVARLLAEKFNKRIVKPE CCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCC PVRGYSEFRNPFTYERFYSSEIKVGTFEAGENHPVRVETVLPFENSNSFLANIAKLYQYG CCCCHHHHCCCCCHHHHHCCCCEEEEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHCC KSFSIEPESILIDSPSPDQLKEISEAAAALSIPVGILLGKNVSLNEKLQNELRGFPKVVF CCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHCCCCHHHH DPFLQFQDGKKMLSFLQERQNAGLYTEIHTSGAKIESFKGLPETLSEIGIKNVLFSIESK HHHHHHCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHCCCCHHHHHHHCHHHHHHCCCCH EILYDYRKLGSILSQHEFPILLHGSFSNPEEALYDSAIGIGGLLIDGIGDLIRIKTPKMK HHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHCCCHHHHHCCCCEEEECCCCHH DIEEIFQLSYDLLQGTRLRLTKTEYISCPSCGRTLFNLQETTARIKSRTGHLKGVKIAVM HHHHHHHHHHHHHCCCEEEEEECCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEE GCIVNGPGEMADADFGYVGAGPGKVHLYRGKEIVMKNVPSEVADEKLVELIKKHGLWQDV EEEECCCCCCCCCCCCEECCCCCEEEEEECCCHHHHCCCHHHHHHHHHHHHHHCCCHHHH INV HCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA