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The map label for this gene is tgt

Identifier: 116328860

GI number: 116328860

Start: 2644834

End: 2645958

Strand: Reverse

Name: tgt

Synonym: LBL_2244

Alternate gene names: 116328860

Gene position: 2645958-2644834 (Counterclockwise)

Preceding gene: 116328861

Following gene: 116328859

Centisome position: 73.21

GC content: 41.51

Gene sequence:

>1125_bases
ATGATTTTTCAAACCACTTCCGAAGATCTTCGAACTAGAGCCAGAACCGGAATTCTAAATCTCAACGGAGTGAAGCTGGA
AACTCCGGTGTTTATGCCCGTAGGGACAAGAGGGGTCGTAAAGACCATTTCTGCCGACGATCTGGAAGAGCTTGAGTATT
CTTTAATATTAGGAAATACGTATCATCTTTATCTCAGACCCGGCACTGCCGTGTTGGAACGTTTTGGGGGACTTAAGAAA
TTTTCGACCTGGAAACGGGCGCTTCTTACCGACAGCGGAGGTTATCAGGTCTTCAGTCTTAATTCTCTATTTAAGTACGA
ACAAGACGGGGTTCGTTTTCAATCTCACATCGACGGAAGCAGGCATTATTTTACTCCTAATTCAGTAATCGACATACAAA
GGACTATCGGTTCCGATATCATGATGGTATTGGACGATTGCGCTCCTTTTGATTCCAGTCAGGAAAGACTAAGACAATCT
TTGGACCGAACACATCGATGGGCGGAAATGTCGGTGGAATATTGGGAAAAGAATAAGAATTCCTCTCATCTTTTCGGAAT
CTTTCAAGGTGGAATTGATCTTGGTCTTCGATTGGAAAGTTTGCAAAAGATCGTCTCTCTCCCATTCGATGGAATTGCAA
TCGGAGGGCTTTCGGTCGGAGAACCACGTAAGGATTTCATCCGAATTTTAGAAGGAGTTTCCGCTTATACGGATCGAAGT
CGCCCTCTCTATTTGATGGGAGTAGGAACCGTTCCAGATATTTTGGATGGAGTAAAGAATGGAGTTGATATGTTTGACTG
CGTGCTTCCGACTCGAAATGCAAGGAACGGTCAAGTTTTTACATCTTTAGGAAAAATTAATCTTAGAAATGAAAAATGGA
AGAGTTCCGATGTGCCTATAGATCCGCATTGCGGTTGTAAGGTGTGCAAAAGATACAGTATCGGATATATCAGACACTTA
CATCACGTGGGGGAACTTACTGCATTTTCTCTCTCAACATATCATAATTTGTATTTTATGAAAAATTTTCTTTCAGAAAT
TCGGAAGTCCATTCAAGCGGGTGAATTTTTAAAGATCTATGCCAAGTGGAAAAATTTGTATGAAAAGCCTGAATTTTCCG
GTTGA

Upstream 100 bases:

>100_bases
TCGAACAACTTCAAGAGCAGGCCGCAAGAGAAAACGGTTTTAAAATCACGGGTCATAGTCTTAACATCTATGGAACTTGC
AACGAACACACTGCCGGTAG

Downstream 100 bases:

>100_bases
CTATTTAAAGAATCGACTTTCCCTTAGTAAGCTGGCTGGAAAGAAATAGGAAGGAGTTGCTGAGCATTTATGGAAATAAC
CAGAAGAGAAAGCGGGAACA

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MIFQTTSEDLRTRARTGILNLNGVKLETPVFMPVGTRGVVKTISADDLEELEYSLILGNTYHLYLRPGTAVLERFGGLKK
FSTWKRALLTDSGGYQVFSLNSLFKYEQDGVRFQSHIDGSRHYFTPNSVIDIQRTIGSDIMMVLDDCAPFDSSQERLRQS
LDRTHRWAEMSVEYWEKNKNSSHLFGIFQGGIDLGLRLESLQKIVSLPFDGIAIGGLSVGEPRKDFIRILEGVSAYTDRS
RPLYLMGVGTVPDILDGVKNGVDMFDCVLPTRNARNGQVFTSLGKINLRNEKWKSSDVPIDPHCGCKVCKRYSIGYIRHL
HHVGELTAFSLSTYHNLYFMKNFLSEIRKSIQAGEFLKIYAKWKNLYEKPEFSG

Sequences:

>Translated_374_residues
MIFQTTSEDLRTRARTGILNLNGVKLETPVFMPVGTRGVVKTISADDLEELEYSLILGNTYHLYLRPGTAVLERFGGLKK
FSTWKRALLTDSGGYQVFSLNSLFKYEQDGVRFQSHIDGSRHYFTPNSVIDIQRTIGSDIMMVLDDCAPFDSSQERLRQS
LDRTHRWAEMSVEYWEKNKNSSHLFGIFQGGIDLGLRLESLQKIVSLPFDGIAIGGLSVGEPRKDFIRILEGVSAYTDRS
RPLYLMGVGTVPDILDGVKNGVDMFDCVLPTRNARNGQVFTSLGKINLRNEKWKSSDVPIDPHCGCKVCKRYSIGYIRHL
HHVGELTAFSLSTYHNLYFMKNFLSEIRKSIQAGEFLKIYAKWKNLYEKPEFSG
>Mature_374_residues
MIFQTTSEDLRTRARTGILNLNGVKLETPVFMPVGTRGVVKTISADDLEELEYSLILGNTYHLYLRPGTAVLERFGGLKK
FSTWKRALLTDSGGYQVFSLNSLFKYEQDGVRFQSHIDGSRHYFTPNSVIDIQRTIGSDIMMVLDDCAPFDSSQERLRQS
LDRTHRWAEMSVEYWEKNKNSSHLFGIFQGGIDLGLRLESLQKIVSLPFDGIAIGGLSVGEPRKDFIRILEGVSAYTDRS
RPLYLMGVGTVPDILDGVKNGVDMFDCVLPTRNARNGQVFTSLGKINLRNEKWKSSDVPIDPHCGCKVCKRYSIGYIRHL
HHVGELTAFSLSTYHNLYFMKNFLSEIRKSIQAGEFLKIYAKWKNLYEKPEFSG

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family

Homologues:

Organism=Homo sapiens, GI13654276, Length=355, Percent_Identity=38.3098591549296, Blast_Score=263, Evalue=2e-70,
Organism=Escherichia coli, GI1786607, Length=356, Percent_Identity=42.6966292134831, Blast_Score=315, Evalue=3e-87,
Organism=Caenorhabditis elegans, GI17544680, Length=352, Percent_Identity=36.9318181818182, Blast_Score=229, Evalue=2e-60,
Organism=Drosophila melanogaster, GI19920510, Length=371, Percent_Identity=40.1617250673854, Blast_Score=294, Evalue=6e-80,
Organism=Drosophila melanogaster, GI21355311, Length=220, Percent_Identity=24.0909090909091, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGT_LEPBJ (Q04UC6)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_800252.1
- ProteinModelPortal:   Q04UC6
- SMR:   Q04UC6
- STRING:   Q04UC6
- GeneID:   4409734
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_0838
- eggNOG:   COG0343
- HOGENOM:   HBG629929
- OMA:   FMPVGTV
- ProtClustDB:   PRK00112
- BioCyc:   LBOR355277:LBJ_0838-MONOMER
- HAMAP:   MF_00168
- InterPro:   IPR004803
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00430
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: =2.4.2.29

Molecular weight: Translated: 42479; Mature: 42479

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: NA

Important sites: ACT_SITE 91-91 BINDING 92-92

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFQTTSEDLRTRARTGILNLNGVKLETPVFMPVGTRGVVKTISADDLEELEYSLILGNT
CCCCCCHHHHHHHHHHCEEECCCEEEECCEEEECCCCHHEEECCCCCHHHHEEEEEECCE
YHLYLRPGTAVLERFGGLKKFSTWKRALLTDSGGYQVFSLNSLFKYEQDGVRFQSHIDGS
EEEEECCCHHHHHHHCCCHHHHHHHHHHEECCCCEEEEEEHHHHHHCCCCCEEHHCCCCC
RHYFTPNSVIDIQRTIGSDIMMVLDDCAPFDSSQERLRQSLDRTHRWAEMSVEYWEKNKN
CEEECCCCEEHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SSHLFGIFQGGIDLGLRLESLQKIVSLPFDGIAIGGLSVGEPRKDFIRILEGVSAYTDRS
CCEEEEEEECCCCCCEEHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCC
RPLYLMGVGTVPDILDGVKNGVDMFDCVLPTRNARNGQVFTSLGKINLRNEKWKSSDVPI
CCEEEEECCCHHHHHHHHHCCCHHHHHHCCCCCCCCCCEEEEECCEEECCCCCCCCCCCC
DPHCGCKVCKRYSIGYIRHLHHVGELTAFSLSTYHNLYFMKNFLSEIRKSIQAGEFLKIY
CCCCCHHHHHHCCHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
AKWKNLYEKPEFSG
HHHHHHHCCCCCCC
>Mature Secondary Structure
MIFQTTSEDLRTRARTGILNLNGVKLETPVFMPVGTRGVVKTISADDLEELEYSLILGNT
CCCCCCHHHHHHHHHHCEEECCCEEEECCEEEECCCCHHEEECCCCCHHHHEEEEEECCE
YHLYLRPGTAVLERFGGLKKFSTWKRALLTDSGGYQVFSLNSLFKYEQDGVRFQSHIDGS
EEEEECCCHHHHHHHCCCHHHHHHHHHHEECCCCEEEEEEHHHHHHCCCCCEEHHCCCCC
RHYFTPNSVIDIQRTIGSDIMMVLDDCAPFDSSQERLRQSLDRTHRWAEMSVEYWEKNKN
CEEECCCCEEHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SSHLFGIFQGGIDLGLRLESLQKIVSLPFDGIAIGGLSVGEPRKDFIRILEGVSAYTDRS
CCEEEEEEECCCCCCEEHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCC
RPLYLMGVGTVPDILDGVKNGVDMFDCVLPTRNARNGQVFTSLGKINLRNEKWKSSDVPI
CCEEEEECCCHHHHHHHHHCCCHHHHHHCCCCCCCCCCEEEEECCEEECCCCCCCCCCCC
DPHCGCKVCKRYSIGYIRHLHHVGELTAFSLSTYHNLYFMKNFLSEIRKSIQAGEFLKIY
CCCCCHHHHHHCCHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
AKWKNLYEKPEFSG
HHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA