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The map label for this gene is purB [H]

Identifier: 116328853

GI number: 116328853

Start: 2633900

End: 2635201

Strand: Reverse

Name: purB [H]

Synonym: LBL_2237

Alternate gene names: 116328853

Gene position: 2635201-2633900 (Counterclockwise)

Preceding gene: 116328854

Following gene: 116328852

Centisome position: 72.91

GC content: 44.01

Gene sequence:

>1302_bases
ATGATCGATCGTTATTCCAATCCCGAAATTTCCAAAATTTGGGAATTAGAGAACAAGTTTGAAATTTGGAAGGAAATAGA
AATACTCGCTTGTGAGATCCGAATGAAACGGGGAGAAATTCCGCCGGAGGAATTTCAGGAAATCAAAACGATGGCAAAGT
TCAATGTAGGCGAAATTCTAGAAATCGAAAGTAAGGTTCATCACGATGTGATTGCGTTTTTAACGAACATGAATTCATAC
ATCGGGCCGGCGGGAAGGCACGTTCATTACGGTCTGACTTCCTCCGATATCGGAGATACTGCGCTCTGCGTGCAGATGGT
TCAGGCTATGGACTTGATCTTGAAAAAAACGGACGCACTCATCTCCGTAGTAAAAGAAAAGGCGATTCAATATAGGGATC
TGCCTTGTATCGGACGTTCCCATGGGATTCATGCGGAACCGATGACCCTTGGGCTCAAATTCGCATTATTTTTTGAAGAA
TTAAATCGAAACCGAAAAAGGATGTCCGAAGCGAGAAACGAGATTGCGGTCGGAAAACTTTCAGGGGCTGTCGGGACTTA
TTCCAATGTCGATCCGGAAATCGAAGCCTATGTTTGCGAAAAGATGGGTTTGAAAGTTGATCCGATCTCCACACAAGTCG
TTTCCAGGGATCGCCACGCGTTTTATCTTTCCGTTCTCGGGGTTACGGCTTCTTCTTTGGATCGTATGGCCACGGAGATC
CGTCTATTACAAAAGACGGAGGGAAGAGAAGTGGAAGAGCCTTTCTCCGCGGGACAAAAGGGTTCATCTGCGATGCCTCA
TAAAAGGAATCCTGTGATCTGTGAGAGGATTTCCGGACTTTCCAGAGTAATCCGCGCAAATGTAAACGTGGGATTACAGG
ATGTAGCTCTTTGGCATGAAAGAGATATTTCTCATTCTTCGGCGGAAAGGGTTGTACTACCCGATTCCACCATCGCACTC
GAATACATTTTGGATAAGATGTTATTTGTGGTTAAAAATCTTCATGTGTATCCGGATGCGATCGAACGAACGTTAGGTGT
CACACGGGGATTGATTTTTTCTCAAAAAGTTTTGCTGGCTTTGATCGAAAGAGGAAAGATCGCAAGAGAAGAAGCTTATC
TAATCGTACAAGAGCACGCGATGGCGGTTTGGGCCAATCCGTCTGAAACTCTCAAAACAAGACTAGAAAACGACAATCGA
GTCGGTCAGGTTTTAACCCGAAAAGATCTCGAAGATATTTTTAAAATCGAACCGTATCTTGAAAAAGTGGGATTGATTTA
CAGACGTTTGGGTCTTGAATAG

Upstream 100 bases:

>100_bases
GGTCGTGTCTCAAACGCTTTTTTTACGGACTTTGTTTTTCTTTTTACAGAATATACTTCCGTAGAAGAGTGGATGAGAAT
TCAAAAAGGAACTTTTAGAA

Downstream 100 bases:

>100_bases
ATGCGGGTCGGAATCTTCGGTTTAGGATATACCGGGATCCGAATTGCGAAAAGATTAAAGTTGCAAGCGGAGATCCGACT
CGAAACTTTTTCTGCCAAAA

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MIDRYSNPEISKIWELENKFEIWKEIEILACEIRMKRGEIPPEEFQEIKTMAKFNVGEILEIESKVHHDVIAFLTNMNSY
IGPAGRHVHYGLTSSDIGDTALCVQMVQAMDLILKKTDALISVVKEKAIQYRDLPCIGRSHGIHAEPMTLGLKFALFFEE
LNRNRKRMSEARNEIAVGKLSGAVGTYSNVDPEIEAYVCEKMGLKVDPISTQVVSRDRHAFYLSVLGVTASSLDRMATEI
RLLQKTEGREVEEPFSAGQKGSSAMPHKRNPVICERISGLSRVIRANVNVGLQDVALWHERDISHSSAERVVLPDSTIAL
EYILDKMLFVVKNLHVYPDAIERTLGVTRGLIFSQKVLLALIERGKIAREEAYLIVQEHAMAVWANPSETLKTRLENDNR
VGQVLTRKDLEDIFKIEPYLEKVGLIYRRLGLE

Sequences:

>Translated_433_residues
MIDRYSNPEISKIWELENKFEIWKEIEILACEIRMKRGEIPPEEFQEIKTMAKFNVGEILEIESKVHHDVIAFLTNMNSY
IGPAGRHVHYGLTSSDIGDTALCVQMVQAMDLILKKTDALISVVKEKAIQYRDLPCIGRSHGIHAEPMTLGLKFALFFEE
LNRNRKRMSEARNEIAVGKLSGAVGTYSNVDPEIEAYVCEKMGLKVDPISTQVVSRDRHAFYLSVLGVTASSLDRMATEI
RLLQKTEGREVEEPFSAGQKGSSAMPHKRNPVICERISGLSRVIRANVNVGLQDVALWHERDISHSSAERVVLPDSTIAL
EYILDKMLFVVKNLHVYPDAIERTLGVTRGLIFSQKVLLALIERGKIAREEAYLIVQEHAMAVWANPSETLKTRLENDNR
VGQVLTRKDLEDIFKIEPYLEKVGLIYRRLGLE
>Mature_433_residues
MIDRYSNPEISKIWELENKFEIWKEIEILACEIRMKRGEIPPEEFQEIKTMAKFNVGEILEIESKVHHDVIAFLTNMNSY
IGPAGRHVHYGLTSSDIGDTALCVQMVQAMDLILKKTDALISVVKEKAIQYRDLPCIGRSHGIHAEPMTLGLKFALFFEE
LNRNRKRMSEARNEIAVGKLSGAVGTYSNVDPEIEAYVCEKMGLKVDPISTQVVSRDRHAFYLSVLGVTASSLDRMATEI
RLLQKTEGREVEEPFSAGQKGSSAMPHKRNPVICERISGLSRVIRANVNVGLQDVALWHERDISHSSAERVVLPDSTIAL
EYILDKMLFVVKNLHVYPDAIERTLGVTRGLIFSQKVLLALIERGKIAREEAYLIVQEHAMAVWANPSETLKTRLENDNR
VGQVLTRKDLEDIFKIEPYLEKVGLIYRRLGLE

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI183227688, Length=399, Percent_Identity=28.0701754385965, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI4557269, Length=379, Percent_Identity=28.7598944591029, Blast_Score=155, Evalue=5e-38,
Organism=Escherichia coli, GI1787376, Length=291, Percent_Identity=27.4914089347079, Blast_Score=89, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17508577, Length=399, Percent_Identity=27.3182957393484, Blast_Score=118, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI32564234, Length=345, Percent_Identity=28.695652173913, Blast_Score=105, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6323391, Length=388, Percent_Identity=29.8969072164948, Blast_Score=169, Evalue=6e-43,
Organism=Drosophila melanogaster, GI24647570, Length=397, Percent_Identity=24.9370277078086, Blast_Score=127, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 49082; Mature: 49082

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDRYSNPEISKIWELENKFEIWKEIEILACEIRMKRGEIPPEEFQEIKTMAKFNVGEIL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEHHHHCCCCCHHHHHHHHHHHHCCCCHHH
EIESKVHHDVIAFLTNMNSYIGPAGRHVHYGLTSSDIGDTALCVQMVQAMDLILKKTDAL
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ISVVKEKAIQYRDLPCIGRSHGIHAEPMTLGLKFALFFEELNRNRKRMSEARNEIAVGKL
HHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHH
SGAVGTYSNVDPEIEAYVCEKMGLKVDPISTQVVSRDRHAFYLSVLGVTASSLDRMATEI
CCCCCCCCCCCCHHHHHHHHHCCCEECCCHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHH
RLLQKTEGREVEEPFSAGQKGSSAMPHKRNPVICERISGLSRVIRANVNVGLQDVALWHE
HHHHHCCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
RDISHSSAERVVLPDSTIALEYILDKMLFVVKNLHVYPDAIERTLGVTRGLIFSQKVLLA
HCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
LIERGKIAREEAYLIVQEHAMAVWANPSETLKTRLENDNRVGQVLTRKDLEDIFKIEPYL
HHHCCCCCHHHHEEEEEECCEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
EKVGLIYRRLGLE
HHHHHHHHHHCCC
>Mature Secondary Structure
MIDRYSNPEISKIWELENKFEIWKEIEILACEIRMKRGEIPPEEFQEIKTMAKFNVGEIL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEHHHHCCCCCHHHHHHHHHHHHCCCCHHH
EIESKVHHDVIAFLTNMNSYIGPAGRHVHYGLTSSDIGDTALCVQMVQAMDLILKKTDAL
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ISVVKEKAIQYRDLPCIGRSHGIHAEPMTLGLKFALFFEELNRNRKRMSEARNEIAVGKL
HHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHH
SGAVGTYSNVDPEIEAYVCEKMGLKVDPISTQVVSRDRHAFYLSVLGVTASSLDRMATEI
CCCCCCCCCCCCHHHHHHHHHCCCEECCCHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHH
RLLQKTEGREVEEPFSAGQKGSSAMPHKRNPVICERISGLSRVIRANVNVGLQDVALWHE
HHHHHCCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
RDISHSSAERVVLPDSTIALEYILDKMLFVVKNLHVYPDAIERTLGVTRGLIFSQKVLLA
HCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
LIERGKIAREEAYLIVQEHAMAVWANPSETLKTRLENDNRVGQVLTRKDLEDIFKIEPYL
HHHCCCCCHHHHEEEEEECCEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
EKVGLIYRRLGLE
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]