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The map label for this gene is dut

Identifier: 116328847

GI number: 116328847

Start: 2625349

End: 2625786

Strand: Direct

Name: dut

Synonym: LBL_2228

Alternate gene names: 116328847

Gene position: 2625349-2625786 (Clockwise)

Preceding gene: 116328846

Following gene: 116328848

Centisome position: 72.63

GC content: 39.5

Gene sequence:

>438_bases
ATGAAAATCCCGATTAAAAAATTAAGATCAAATGCCGAACTTCCGGTATTACAAACAAAACATGCAGCGGGTTATGACGT
TCACGCTTGTTTGGATTCAAATCTAATCTTGGAACCGAACAAAGTGACTCTCGTTCCAACCGGACTTTCTTTTGAAATTC
CTCAAGAATATCATTTTGAAATTAGGCCTAGATCGGGATTCTCGACGAAAAATCAAATCTTAATTCCAAATTCTCCCGGA
ACAATCGACAGCGATTATCGAGGAGAATTGATGATTCCTCTTTTCAATTTAGGAAACATTCCCTTTGTAATCGAACATGG
GATGAGAATCGCTCAACTGCTCATTCGTGAAACTCATTATACAAATTGGGAACTGGTTTCCGAATTTACGGATACGACAG
AAAGAGGAACAGGCGGATTCGGCTCTACCGGTCATTAG

Upstream 100 bases:

>100_bases
AAAAATTGTTATCGAAGTTCTGGAAAAATTATGGGATTTTCTTTTTCTATCGAAATAAGAAAATCTCTTCGTTTTTAAAA
GTAGGAGAATCAATGAACAT

Downstream 100 bases:

>100_bases
AAACCGCCCCAATTGCCATATTTTTTAGAAAAAAATAATATACTAAAATATCCATAATGCTCGCTTCCATCAGGATTCGT
TCCGGAAATGAACCAAAAAC

Product: dUTP diphosphatase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 145; Mature: 145

Protein sequence:

>145_residues
MKIPIKKLRSNAELPVLQTKHAAGYDVHACLDSNLILEPNKVTLVPTGLSFEIPQEYHFEIRPRSGFSTKNQILIPNSPG
TIDSDYRGELMIPLFNLGNIPFVIEHGMRIAQLLIRETHYTNWELVSEFTDTTERGTGGFGSTGH

Sequences:

>Translated_145_residues
MKIPIKKLRSNAELPVLQTKHAAGYDVHACLDSNLILEPNKVTLVPTGLSFEIPQEYHFEIRPRSGFSTKNQILIPNSPG
TIDSDYRGELMIPLFNLGNIPFVIEHGMRIAQLLIRETHYTNWELVSEFTDTTERGTGGFGSTGH
>Mature_145_residues
MKIPIKKLRSNAELPVLQTKHAAGYDVHACLDSNLILEPNKVTLVPTGLSFEIPQEYHFEIRPRSGFSTKNQILIPNSPG
TIDSDYRGELMIPLFNLGNIPFVIEHGMRIAQLLIRETHYTNWELVSEFTDTTERGTGGFGSTGH

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Homo sapiens, GI70906444, Length=144, Percent_Identity=36.8055555555556, Blast_Score=95, Evalue=2e-20,
Organism=Homo sapiens, GI4503423, Length=145, Percent_Identity=36.551724137931, Blast_Score=94, Evalue=3e-20,
Organism=Homo sapiens, GI70906441, Length=144, Percent_Identity=36.8055555555556, Blast_Score=94, Evalue=5e-20,
Organism=Escherichia coli, GI1790071, Length=149, Percent_Identity=42.2818791946309, Blast_Score=103, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI71988561, Length=145, Percent_Identity=36.551724137931, Blast_Score=95, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6319729, Length=140, Percent_Identity=36.4285714285714, Blast_Score=88, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24583610, Length=138, Percent_Identity=37.6811594202899, Blast_Score=95, Evalue=1e-20,
Organism=Drosophila melanogaster, GI19921126, Length=138, Percent_Identity=37.6811594202899, Blast_Score=95, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUT_LEPBJ (Q04QV4)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_801474.1
- ProteinModelPortal:   Q04QV4
- SMR:   Q04QV4
- STRING:   Q04QV4
- GeneID:   4411438
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_2235
- eggNOG:   COG0756
- HOGENOM:   HBG436079
- OMA:   GTIDEGY
- PhylomeDB:   Q04QV4
- ProtClustDB:   CLSK573773
- BioCyc:   LBOR355277:LBJ_2235-MONOMER
- HAMAP:   MF_00116
- InterPro:   IPR008180
- InterPro:   IPR008181
- TIGRFAMs:   TIGR00576

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 16262; Mature: 16262

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: BINDING 77-77 BINDING 91-91

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIPIKKLRSNAELPVLQTKHAAGYDVHACLDSNLILEPNKVTLVPTGLSFEIPQEYHFE
CCCCHHHHHCCCCCCEEEECCCCCCEEEEECCCCEEECCCCEEEEECCCEEECCCCEEEE
IRPRSGFSTKNQILIPNSPGTIDSDYRGELMIPLFNLGNIPFVIEHGMRIAQLLIRETHY
EECCCCCCCCCEEEECCCCCCCCCCCCCEEEEEEECCCCCCEEECCCHHHHHHHHHHCCC
TNWELVSEFTDTTERGTGGFGSTGH
CCHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKIPIKKLRSNAELPVLQTKHAAGYDVHACLDSNLILEPNKVTLVPTGLSFEIPQEYHFE
CCCCHHHHHCCCCCCEEEECCCCCCEEEEECCCCEEECCCCEEEEECCCEEECCCCEEEE
IRPRSGFSTKNQILIPNSPGTIDSDYRGELMIPLFNLGNIPFVIEHGMRIAQLLIRETHY
EECCCCCCCCCEEEECCCCCCCCCCCCCEEEEEEECCCCCCEEECCCHHHHHHHHHHCCC
TNWELVSEFTDTTERGTGGFGSTGH
CCHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA