Definition Leptospira borgpetersenii serovar Hardjo-bovis L550 chromosome 1, complete sequence.
Accession NC_008508
Length 3,614,446

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The map label for this gene is glyRS

Identifier: 116328846

GI number: 116328846

Start: 2623663

End: 2625057

Strand: Direct

Name: glyRS

Synonym: LBL_2227

Alternate gene names: 116328846

Gene position: 2623663-2625057 (Clockwise)

Preceding gene: 116328841

Following gene: 116328847

Centisome position: 72.59

GC content: 43.66

Gene sequence:

>1395_bases
ATGGAAAAGAAAGAAAGTCTCGATTCTTCATTGAAAGAAATCGTATCCGTCTGCAAACGTAGAGGATTTGTCTATCCCGG
TTCTGAAATTTACGGAGGACTTTCCAACACTTTCGACTATGGTCCTTACGGCGTAGAACTTCTCCAAAATCTGAAACAGC
TCTGGTGGAAATTTTTTGTACATCTCCGAGAAGACGTGGTAGGTCTCGATTCCTCCATTCTTCTCAATCCGAAAGTTTGG
GAAGCATCCGGTCACGTTTCCAACTTCACCGATCCTTTGATTGATTGTAAAAATTGTAAGACCCGAATCCGTGCGGATAA
GTTCCTAGAGGATCAAAAAGGAGAAGGATTCGCGACCGGTCTCACCCTCGAAAAGATGAATCAAGTCATCAAAGAAAATA
ATTTCTCCTGCCCGAACTGCGGTCAAAGAGGAACATTTACCGAAGCACGAGACTTCAATCTCATGTTCAAAACTTCCCAT
GGCGCGAGCGCGGAAGATTCCTTGGATATTTATCTCAGACCCGAAACCGCTCAAGGAATTTTTTTAAATTTTAAGAACGT
AGTCTCCACCACCAGAAGAAAGATTCCGTTCGGAATCGCCCAGATCGGTAAATCCTTTCGGAACGAAATCATGGCGCGAC
AATTCGTTTTTAGAACGAGAGAATTCGAGCAGATGGAAATGGAATTCTTTTGCGAACCGGGAACCCAGAAAGAATGGTTT
TCTCACTGGGTGAATTATTGTATGAATTGGCTGACCGAACAGGTTGGGGTCAAAAAGGAAAATTTAAGAATCAGAGAACA
CGAAAAAGAAGAATTATCTTTTTATAGCGAAGGCACTTCCGATATCGAATTCAAATACAACTTCGGCTGGGGAGAACTCT
GGGGAATCGCTTCCAGAACCGACTACGATTTGAACCAACATCAAAAATTCTCCGGTGAAGACCTGAAATACCAGGATCAG
GTTCAGAATAAAAAATACGTCCCTTTTGTCGTTGAACCCGCGTTAGGTGTAAACAGACTTTTTCTTGCCGTCGTTACCGA
CGCTTACCAAGAGGAAAAACTTCCCGATGGAGAAATAAGAACCGTCCTTCGCTTTTCTCCCAAGATCGCCCCGGTTAAAG
TGGCCGTTTTTCCTTTGATGAAAAAAGACGGGTTACTTGAAAAATCCAGAGAAATTTTCGCGGATCTTTCCAAACTCGGA
AATATTGAATACGACGATAGCGGCGCGATCGGGAAACGCTATCGTAGACAGGACGAAATCGGAACCCCTTTTTGTATTAC
AGTAGATTATGATACTCTAAAGGACGACACGGTTACCGTTAGAGAAAGGGACAGCATGGCTCAGGAAAGAGTTTCGGTGA
CCCGACTCAGGAACTGGCTCTTTGAGAGGCTATGA

Upstream 100 bases:

>100_bases
CAACCATTGGACCCGGATAAGGAGCACAGGCAAACAGAAATCCTTCGAAGAGAAGAATTAAAATACTTTATTCCGAAGTC
CCAAACGAAATTCTGGATGT

Downstream 100 bases:

>100_bases
AACGGGGATCCGTTCGATCCATAAACCATATGATCGAATCCATGTTATAGTCGATTCTGAGCGCCCGTGAGACAAAGTAT
AGGAAGTTTGTGCCAAAATT

Product: glycyl-tRNA synthetase

Products: NA

Alternate protein names: Glycine--tRNA ligase; GlyRS

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MEKKESLDSSLKEIVSVCKRRGFVYPGSEIYGGLSNTFDYGPYGVELLQNLKQLWWKFFVHLREDVVGLDSSILLNPKVW
EASGHVSNFTDPLIDCKNCKTRIRADKFLEDQKGEGFATGLTLEKMNQVIKENNFSCPNCGQRGTFTEARDFNLMFKTSH
GASAEDSLDIYLRPETAQGIFLNFKNVVSTTRRKIPFGIAQIGKSFRNEIMARQFVFRTREFEQMEMEFFCEPGTQKEWF
SHWVNYCMNWLTEQVGVKKENLRIREHEKEELSFYSEGTSDIEFKYNFGWGELWGIASRTDYDLNQHQKFSGEDLKYQDQ
VQNKKYVPFVVEPALGVNRLFLAVVTDAYQEEKLPDGEIRTVLRFSPKIAPVKVAVFPLMKKDGLLEKSREIFADLSKLG
NIEYDDSGAIGKRYRRQDEIGTPFCITVDYDTLKDDTVTVRERDSMAQERVSVTRLRNWLFERL

Sequences:

>Translated_464_residues
MEKKESLDSSLKEIVSVCKRRGFVYPGSEIYGGLSNTFDYGPYGVELLQNLKQLWWKFFVHLREDVVGLDSSILLNPKVW
EASGHVSNFTDPLIDCKNCKTRIRADKFLEDQKGEGFATGLTLEKMNQVIKENNFSCPNCGQRGTFTEARDFNLMFKTSH
GASAEDSLDIYLRPETAQGIFLNFKNVVSTTRRKIPFGIAQIGKSFRNEIMARQFVFRTREFEQMEMEFFCEPGTQKEWF
SHWVNYCMNWLTEQVGVKKENLRIREHEKEELSFYSEGTSDIEFKYNFGWGELWGIASRTDYDLNQHQKFSGEDLKYQDQ
VQNKKYVPFVVEPALGVNRLFLAVVTDAYQEEKLPDGEIRTVLRFSPKIAPVKVAVFPLMKKDGLLEKSREIFADLSKLG
NIEYDDSGAIGKRYRRQDEIGTPFCITVDYDTLKDDTVTVRERDSMAQERVSVTRLRNWLFERL
>Mature_464_residues
MEKKESLDSSLKEIVSVCKRRGFVYPGSEIYGGLSNTFDYGPYGVELLQNLKQLWWKFFVHLREDVVGLDSSILLNPKVW
EASGHVSNFTDPLIDCKNCKTRIRADKFLEDQKGEGFATGLTLEKMNQVIKENNFSCPNCGQRGTFTEARDFNLMFKTSH
GASAEDSLDIYLRPETAQGIFLNFKNVVSTTRRKIPFGIAQIGKSFRNEIMARQFVFRTREFEQMEMEFFCEPGTQKEWF
SHWVNYCMNWLTEQVGVKKENLRIREHEKEELSFYSEGTSDIEFKYNFGWGELWGIASRTDYDLNQHQKFSGEDLKYQDQ
VQNKKYVPFVVEPALGVNRLFLAVVTDAYQEEKLPDGEIRTVLRFSPKIAPVKVAVFPLMKKDGLLEKSREIFADLSKLG
NIEYDDSGAIGKRYRRQDEIGTPFCITVDYDTLKDDTVTVRERDSMAQERVSVTRLRNWLFERL

Specific function: Catalyzes the attachment of glycine to tRNA(Gly)

COG id: COG0423

COG function: function code J; Glycyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI116805340, Length=382, Percent_Identity=30.6282722513089, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI70887790, Length=480, Percent_Identity=22.0833333333333, Blast_Score=88, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI32564541, Length=376, Percent_Identity=32.7127659574468, Blast_Score=167, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI17554996, Length=376, Percent_Identity=32.7127659574468, Blast_Score=167, Evalue=9e-42,
Organism=Saccharomyces cerevisiae, GI6325338, Length=382, Percent_Identity=32.1989528795811, Blast_Score=168, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6319597, Length=407, Percent_Identity=29.7297297297297, Blast_Score=158, Evalue=1e-39,
Organism=Drosophila melanogaster, GI21357965, Length=372, Percent_Identity=32.5268817204301, Blast_Score=168, Evalue=7e-42,
Organism=Drosophila melanogaster, GI24664462, Length=373, Percent_Identity=32.171581769437, Blast_Score=168, Evalue=8e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYG_LEPBJ (Q04QV5)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_801473.1
- ProteinModelPortal:   Q04QV5
- SMR:   Q04QV5
- STRING:   Q04QV5
- GeneID:   4411437
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_2234
- eggNOG:   COG0423
- HOGENOM:   HBG319978
- OMA:   GSEWGEL
- PhylomeDB:   Q04QV5
- ProtClustDB:   PRK04173
- BioCyc:   LBOR355277:LBJ_2234-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00253_B
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR022961
- InterPro:   IPR002315
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR10745
- PRINTS:   PR01043
- TIGRFAMs:   TIGR00389

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.14

Molecular weight: Translated: 53887; Mature: 53887

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: BINDING 104-104 BINDING 175-175

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MEKKESLDSSLKEIVSVCKRRGFVYPGSEIYGGLSNTFDYGPYGVELLQNLKQLWWKFFV
CCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
HLREDVVGLDSSILLNPKVWEASGHVSNFTDPLIDCKNCKTRIRADKFLEDQKGEGFATG
HHHHHHHCCCCCEEECCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEC
LTLEKMNQVIKENNFSCPNCGQRGTFTEARDFNLMFKTSHGASAEDSLDIYLRPETAQGI
CCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEECCCCCCEE
FLNFKNVVSTTRRKIPFGIAQIGKSFRNEIMARQFVFRTREFEQMEMEFFCEPGTQKEWF
EEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHH
SHWVNYCMNWLTEQVGVKKENLRIREHEKEELSFYSEGTSDIEFKYNFGWGELWGIASRT
HHHHHHHHHHHHHHHCCCHHCCCHHHCCHHHHHHHCCCCCCEEEEEECCCHHHHCCCCCC
DYDLNQHQKFSGEDLKYQDQVQNKKYVPFVVEPALGVNRLFLAVVTDAYQEEKLPDGEIR
CCCCCCCCCCCCCCCCHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHH
TVLRFSPKIAPVKVAVFPLMKKDGLLEKSREIFADLSKLGNIEYDDSGAIGKRYRRQDEI
HHHHCCCCCCCEEEEEEHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCC
GTPFCITVDYDTLKDDTVTVRERDSMAQERVSVTRLRNWLFERL
CCCEEEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEKKESLDSSLKEIVSVCKRRGFVYPGSEIYGGLSNTFDYGPYGVELLQNLKQLWWKFFV
CCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
HLREDVVGLDSSILLNPKVWEASGHVSNFTDPLIDCKNCKTRIRADKFLEDQKGEGFATG
HHHHHHHCCCCCEEECCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEC
LTLEKMNQVIKENNFSCPNCGQRGTFTEARDFNLMFKTSHGASAEDSLDIYLRPETAQGI
CCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEECCCCCCEE
FLNFKNVVSTTRRKIPFGIAQIGKSFRNEIMARQFVFRTREFEQMEMEFFCEPGTQKEWF
EEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHH
SHWVNYCMNWLTEQVGVKKENLRIREHEKEELSFYSEGTSDIEFKYNFGWGELWGIASRT
HHHHHHHHHHHHHHHCCCHHCCCHHHCCHHHHHHHCCCCCCEEEEEECCCHHHHCCCCCC
DYDLNQHQKFSGEDLKYQDQVQNKKYVPFVVEPALGVNRLFLAVVTDAYQEEKLPDGEIR
CCCCCCCCCCCCCCCCHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHH
TVLRFSPKIAPVKVAVFPLMKKDGLLEKSREIFADLSKLGNIEYDDSGAIGKRYRRQDEI
HHHHCCCCCCCEEEEEEHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCC
GTPFCITVDYDTLKDDTVTVRERDSMAQERVSVTRLRNWLFERL
CCCEEEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA