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The map label for this gene is miaB-2 [H]

Identifier: 116328778

GI number: 116328778

Start: 2525880

End: 2527199

Strand: Reverse

Name: miaB-2 [H]

Synonym: LBL_2153

Alternate gene names: 116328778

Gene position: 2527199-2525880 (Counterclockwise)

Preceding gene: 116328779

Following gene: 116328777

Centisome position: 69.92

GC content: 44.92

Gene sequence:

>1320_bases
TTGAATAAGAAATTCTACATCACCACTCTCGGTTGCCCCAAAAATACCGCGGACTCTATGAGTATGCATCATTCTCTTTT
GGAGGAAGGATTTACTCCGGCCACTTTTGCGGAAGAGTCCGATTTTCACTTCATCAATACCTGCACTTTCATTCAATCTG
CTACGGAGGAAACGATTCAGACGATTTTGTCCGCGGCCCAGGTCAAAAAACAAAATCATCAGAAACTGGTCGTAGTAGGC
TGTTTTGCAGAAAGATATCCGGATAATATCAGTTCCGAAATTCCGGAAGTGGATCTGTTTTTCGGCACAGGCAGATATGC
ACAAGCGGGTAAAATACTGCGGGAAAAATTTCCGGATCTTTCTCCTCCGAAACGGGAATTCAACGATAGTCTATTAGAAA
GATTGAAACTATCTTCCGAGATCGAAAATTATTCCAAACCGTACGCCTATGTGAAAGTTTCCGACGGTTGCAACCGCGGA
TGCTCGTTTTGTATCATTCCTTCTTTTCGAGGTAAGTTTCGGGAATCTCCCGTGGAAGATATTCTTCGGGATGTGGATCG
TGCGATCCGTGCGGGCGCTAAAGAAATTTGTCTGGTTTCCCAAGATACCGTATATTACGGAAGGAATTCCGAAGTCCTTT
TGGATATGGTCCGCAAAGTTGCGGAGATCGATTCCCTGGAAGTTTTAAGACTCTTGTATTTGTACCCGGATAAAAAAACC
GAAAAGTTGCTTCGTCTAATGGGAGAAACTCCGAAAATTGCTCCGTATCTGGAATCCCCTTTGCAACATGTATCTTCCAA
GATTCTAAAATCGATGAATCGAGTCGGGGAAAGTTCCACTTTCAAGGATCTTTTTGCCTTGGCCAGAGAGGTCAAACCCG
GTCTCGAAATTCGGACTTCGTTTATCATCGGTTATCCCGGAGAAGAACCGGGTGATGTGGATCAGGTTCTGCGATTCATT
GAAGAAACGAGACCGGAAAAGGTGAACCTATTTTCCTATTCCCCGCAAGAAGGAACCAAAGGAGCGGAGTTGAAGCAAAC
CGTTTCCGAAAAGGAAAAGTCGAGACGAATCAATCTGATTCGGGATGTACACTTGGCGATCTTAGAGGAGATTCACGAAT
CCCGTATCGGTCAAACCTACGATGCGATTGTGGACTCGGTCGAAAACGACCAGGCCGTCGTTCGCAGGTTTCAGGACGCT
CCCGAAATGGACGAGGTCGTGTATGTGGACGATATTTCCTTAATTCCGGGTATGATCGGAAAAATACGGATCGATTCCTT
TTATGAATACGATATGAACGGAACCTGGATTTCTAAATGA

Upstream 100 bases:

>100_bases
TAGGACGTCCTGGAAAACTCGTGAAGAAGATATTCGTGAAAACGCAGAACCCTTATGATAGTACTCAGTCCATTATCAAG
GAGTTGGGAGAATAGGATTT

Downstream 100 bases:

>100_bases
ACAAAGCAATTTTCAATATTCCTAATATTCTCACGATGCTTCGCGTTGTGGCGGTTCCTTTTTTCGTATGGTTTTTGTTC
CAGAAAGAATTGGAATATCA

Product: 2-methylthioadenine synthetase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO [H]

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MNKKFYITTLGCPKNTADSMSMHHSLLEEGFTPATFAEESDFHFINTCTFIQSATEETIQTILSAAQVKKQNHQKLVVVG
CFAERYPDNISSEIPEVDLFFGTGRYAQAGKILREKFPDLSPPKREFNDSLLERLKLSSEIENYSKPYAYVKVSDGCNRG
CSFCIIPSFRGKFRESPVEDILRDVDRAIRAGAKEICLVSQDTVYYGRNSEVLLDMVRKVAEIDSLEVLRLLYLYPDKKT
EKLLRLMGETPKIAPYLESPLQHVSSKILKSMNRVGESSTFKDLFALAREVKPGLEIRTSFIIGYPGEEPGDVDQVLRFI
EETRPEKVNLFSYSPQEGTKGAELKQTVSEKEKSRRINLIRDVHLAILEEIHESRIGQTYDAIVDSVENDQAVVRRFQDA
PEMDEVVYVDDISLIPGMIGKIRIDSFYEYDMNGTWISK

Sequences:

>Translated_439_residues
MNKKFYITTLGCPKNTADSMSMHHSLLEEGFTPATFAEESDFHFINTCTFIQSATEETIQTILSAAQVKKQNHQKLVVVG
CFAERYPDNISSEIPEVDLFFGTGRYAQAGKILREKFPDLSPPKREFNDSLLERLKLSSEIENYSKPYAYVKVSDGCNRG
CSFCIIPSFRGKFRESPVEDILRDVDRAIRAGAKEICLVSQDTVYYGRNSEVLLDMVRKVAEIDSLEVLRLLYLYPDKKT
EKLLRLMGETPKIAPYLESPLQHVSSKILKSMNRVGESSTFKDLFALAREVKPGLEIRTSFIIGYPGEEPGDVDQVLRFI
EETRPEKVNLFSYSPQEGTKGAELKQTVSEKEKSRRINLIRDVHLAILEEIHESRIGQTYDAIVDSVENDQAVVRRFQDA
PEMDEVVYVDDISLIPGMIGKIRIDSFYEYDMNGTWISK
>Mature_439_residues
MNKKFYITTLGCPKNTADSMSMHHSLLEEGFTPATFAEESDFHFINTCTFIQSATEETIQTILSAAQVKKQNHQKLVVVG
CFAERYPDNISSEIPEVDLFFGTGRYAQAGKILREKFPDLSPPKREFNDSLLERLKLSSEIENYSKPYAYVKVSDGCNRG
CSFCIIPSFRGKFRESPVEDILRDVDRAIRAGAKEICLVSQDTVYYGRNSEVLLDMVRKVAEIDSLEVLRLLYLYPDKKT
EKLLRLMGETPKIAPYLESPLQHVSSKILKSMNRVGESSTFKDLFALAREVKPGLEIRTSFIIGYPGEEPGDVDQVLRFI
EETRPEKVNLFSYSPQEGTKGAELKQTVSEKEKSRRINLIRDVHLAILEEIHESRIGQTYDAIVDSVENDQAVVRRFQDA
PEMDEVVYVDDISLIPGMIGKIRIDSFYEYDMNGTWISK

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12 [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=427, Percent_Identity=23.8875878220141, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI28872782, Length=429, Percent_Identity=23.7762237762238, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI93277076, Length=374, Percent_Identity=24.331550802139, Blast_Score=116, Evalue=4e-26,
Organism=Escherichia coli, GI1787057, Length=446, Percent_Identity=34.304932735426, Blast_Score=237, Evalue=1e-63,
Organism=Escherichia coli, GI1786882, Length=375, Percent_Identity=27.4666666666667, Blast_Score=164, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17553146, Length=395, Percent_Identity=21.5189873417722, Blast_Score=88, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI71996771, Length=248, Percent_Identity=24.1935483870968, Blast_Score=75, Evalue=7e-14,
Organism=Drosophila melanogaster, GI21356207, Length=417, Percent_Identity=22.5419664268585, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI19922432, Length=398, Percent_Identity=23.3668341708543, Blast_Score=105, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840 [H]

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 49985; Mature: 49985

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKFYITTLGCPKNTADSMSMHHSLLEEGFTPATFAEESDFHFINTCTFIQSATEETIQ
CCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHH
TILSAAQVKKQNHQKLVVVGCFAERYPDNISSEIPEVDLFFGTGRYAQAGKILREKFPDL
HHHHHHHHHHCCCCEEEEEEEHHHHCCCCHHHCCCCEEEEECCCCHHHHHHHHHHHCCCC
SPPKREFNDSLLERLKLSSEIENYSKPYAYVKVSDGCNRGCSFCIIPSFRGKFRESPVED
CCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEECCHHCHHCCCCHHH
ILRDVDRAIRAGAKEICLVSQDTVYYGRNSEVLLDMVRKVAEIDSLEVLRLLYLYPDKKT
HHHHHHHHHHCCCHHEEEEECCEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCHHH
EKLLRLMGETPKIAPYLESPLQHVSSKILKSMNRVGESSTFKDLFALAREVKPGLEIRTS
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEE
FIIGYPGEEPGDVDQVLRFIEETRPEKVNLFSYSPQEGTKGAELKQTVSEKEKSRRINLI
EEEECCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
RDVHLAILEEIHESRIGQTYDAIVDSVENDQAVVRRFQDAPEMDEVVYVDDISLIPGMIG
HHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHCCCCCCCEEEECCCHHHCCCCC
KIRIDSFYEYDMNGTWISK
EEEECCEEEECCCCCEECC
>Mature Secondary Structure
MNKKFYITTLGCPKNTADSMSMHHSLLEEGFTPATFAEESDFHFINTCTFIQSATEETIQ
CCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHH
TILSAAQVKKQNHQKLVVVGCFAERYPDNISSEIPEVDLFFGTGRYAQAGKILREKFPDL
HHHHHHHHHHCCCCEEEEEEEHHHHCCCCHHHCCCCEEEEECCCCHHHHHHHHHHHCCCC
SPPKREFNDSLLERLKLSSEIENYSKPYAYVKVSDGCNRGCSFCIIPSFRGKFRESPVED
CCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEECCHHCHHCCCCHHH
ILRDVDRAIRAGAKEICLVSQDTVYYGRNSEVLLDMVRKVAEIDSLEVLRLLYLYPDKKT
HHHHHHHHHHCCCHHEEEEECCEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCHHH
EKLLRLMGETPKIAPYLESPLQHVSSKILKSMNRVGESSTFKDLFALAREVKPGLEIRTS
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEE
FIIGYPGEEPGDVDQVLRFIEETRPEKVNLFSYSPQEGTKGAELKQTVSEKEKSRRINLI
EEEECCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
RDVHLAILEEIHESRIGQTYDAIVDSVENDQAVVRRFQDAPEMDEVVYVDDISLIPGMIG
HHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHCCCCCCCEEEECCCHHHCCCCC
KIRIDSFYEYDMNGTWISK
EEEECCEEEECCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA