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The map label for this gene is aceF [H]

Identifier: 116328748

GI number: 116328748

Start: 2483856

End: 2485097

Strand: Reverse

Name: aceF [H]

Synonym: LBL_2122

Alternate gene names: 116328748

Gene position: 2485097-2483856 (Counterclockwise)

Preceding gene: 116328749

Following gene: 116328747

Centisome position: 68.75

GC content: 47.75

Gene sequence:

>1242_bases
ATGTCTGTAGAAATTAAGGTCCCCGAAATGGGGGAATCCATCACGGAAGCTACGATTGCGAATTGGGTTAAGAAAGAAGG
AGACGCGGTAAAACAAGATGAGATCTTGTTGGAACTGGAAACCGATAAGGCCACTATGGAAGTTCCGGCGCCTTCTTCCG
GCGTACTTCAAAAAATTCATAAGAAGGCCGGAGATACAGTGAAAGTGAAAGAGGTCGTCGGCCTCATCGATTCCGCAGCA
ACCGTCTCCGCACCGGCACCTTCCTCTTCTTCTCCTGCAATCACAACACAAACGAATCAAACCTCGGGAAATGACAGATT
CAATGATACTCTTCCACCTGCGGTTCGTAAATTGATCGACGACAACGGTCTGAATGTTACGGCTATTTCCGGTTCGGGTA
AGAACGGACAGATCACAAAAGAAGACGTATTAAAGGCGATCGAATCCAAAACTTCCGCAGGTGTGGGAACTGCAACGGCT
GCAAAAGCGGCGTCTTCTCCCGAAATTCCGAAAGCGGTTCCTGTTGCAAAAAGAACGGATCTTCCGAGAGAAAATGCGGT
CCCGATGACTCGTTTACGCAAAGTGATCGCGGAAAGACTCGTATCCGCGCAACACAATGCCGCCATTTTAACCACGTTCA
ACGAAGTGGACATGAGCGCCGTAATGGAACTCAGAAATCGTTATAAGGATCGCTTTAAAGAGGCGCATAACGTGGGTCTT
GGATTTATGAGTTTTTTTACGAAAGCTGCCATCCATGCTCTGAAAACGATTCCCGCAATCAACGCGGAAATCCGCGGAAA
CGACGTCGTATATAAGAACTTTTATGATATCGGGGTCGCTGTCGGAGGACCGAAAGGACTTGTGGTGCCGATCGTAAGAG
ATGCGGATCTTTTGAGTTTCGCGGGAGTGGAGCAAGAGATCGTAAGACTTGCCAACCGAGTTAAGGACGGAAAGATCGAA
CTTTCGGAGATGGAGGGCGGAACGTTTACGATTTCCAACGGGGGAATCTACGGTTCCATGATGTCCACTCCGATCTTAAA
CCCTCCTCAAAGTGGAATTTTGGGACTTCATAATATAGTAAAGCGTGCGGTGGTGGTAAACGATCAAATCGTAATCCGTC
CGATGATGTATCTCGCACTTTCCTACGATCACAGAATCGTAGACGGGAAAGAAGCGGTTACATTTCTCGTAAAGGTCAAA
GAAGCGATCGAAGATCCGGCCCGACTTTTACTCGAACTTTAA

Upstream 100 bases:

>100_bases
GTGTTTTAGAGAATTTCGTTTACAGAATTTTTTCATTTAGAAGGATTGAAACCGAGACTTACGCTTTTTTGTATCTTAAG
CCTCTTGAAAGGAATCATTC

Downstream 100 bases:

>100_bases
TGGAAGTGGAAACATGTCAGCAGAATTCGACGTAGTCGTGATCGGTGCGGGACCAGGGGGCTACGTTTGCGCCATCCGGG
CCGCTCAACTCGGTTTCAAA

Product: bifunctional dihydrolipoyllysine-residue acetyltransferase/dihydrolipoyllysine-residue succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MSVEIKVPEMGESITEATIANWVKKEGDAVKQDEILLELETDKATMEVPAPSSGVLQKIHKKAGDTVKVKEVVGLIDSAA
TVSAPAPSSSSPAITTQTNQTSGNDRFNDTLPPAVRKLIDDNGLNVTAISGSGKNGQITKEDVLKAIESKTSAGVGTATA
AKAASSPEIPKAVPVAKRTDLPRENAVPMTRLRKVIAERLVSAQHNAAILTTFNEVDMSAVMELRNRYKDRFKEAHNVGL
GFMSFFTKAAIHALKTIPAINAEIRGNDVVYKNFYDIGVAVGGPKGLVVPIVRDADLLSFAGVEQEIVRLANRVKDGKIE
LSEMEGGTFTISNGGIYGSMMSTPILNPPQSGILGLHNIVKRAVVVNDQIVIRPMMYLALSYDHRIVDGKEAVTFLVKVK
EAIEDPARLLLEL

Sequences:

>Translated_413_residues
MSVEIKVPEMGESITEATIANWVKKEGDAVKQDEILLELETDKATMEVPAPSSGVLQKIHKKAGDTVKVKEVVGLIDSAA
TVSAPAPSSSSPAITTQTNQTSGNDRFNDTLPPAVRKLIDDNGLNVTAISGSGKNGQITKEDVLKAIESKTSAGVGTATA
AKAASSPEIPKAVPVAKRTDLPRENAVPMTRLRKVIAERLVSAQHNAAILTTFNEVDMSAVMELRNRYKDRFKEAHNVGL
GFMSFFTKAAIHALKTIPAINAEIRGNDVVYKNFYDIGVAVGGPKGLVVPIVRDADLLSFAGVEQEIVRLANRVKDGKIE
LSEMEGGTFTISNGGIYGSMMSTPILNPPQSGILGLHNIVKRAVVVNDQIVIRPMMYLALSYDHRIVDGKEAVTFLVKVK
EAIEDPARLLLEL
>Mature_412_residues
SVEIKVPEMGESITEATIANWVKKEGDAVKQDEILLELETDKATMEVPAPSSGVLQKIHKKAGDTVKVKEVVGLIDSAAT
VSAPAPSSSSPAITTQTNQTSGNDRFNDTLPPAVRKLIDDNGLNVTAISGSGKNGQITKEDVLKAIESKTSAGVGTATAA
KAASSPEIPKAVPVAKRTDLPRENAVPMTRLRKVIAERLVSAQHNAAILTTFNEVDMSAVMELRNRYKDRFKEAHNVGLG
FMSFFTKAAIHALKTIPAINAEIRGNDVVYKNFYDIGVAVGGPKGLVVPIVRDADLLSFAGVEQEIVRLANRVKDGKIEL
SEMEGGTFTISNGGIYGSMMSTPILNPPQSGILGLHNIVKRAVVVNDQIVIRPMMYLALSYDHRIVDGKEAVTFLVKVKE
AIEDPARLLLEL

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and l

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=257, Percent_Identity=49.0272373540856, Blast_Score=261, Evalue=6e-70,
Organism=Homo sapiens, GI110671329, Length=422, Percent_Identity=29.1469194312796, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI203098753, Length=456, Percent_Identity=28.9473684210526, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI203098816, Length=456, Percent_Identity=28.9473684210526, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI31711992, Length=448, Percent_Identity=25.8928571428571, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI260898739, Length=160, Percent_Identity=33.125, Blast_Score=87, Evalue=4e-17,
Organism=Escherichia coli, GI1786946, Length=414, Percent_Identity=46.3768115942029, Blast_Score=385, Evalue=1e-108,
Organism=Escherichia coli, GI1786305, Length=429, Percent_Identity=27.5058275058275, Blast_Score=168, Evalue=7e-43,
Organism=Caenorhabditis elegans, GI25146366, Length=417, Percent_Identity=40.5275779376499, Blast_Score=291, Evalue=3e-79,
Organism=Caenorhabditis elegans, GI17537937, Length=434, Percent_Identity=27.4193548387097, Blast_Score=158, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI17560088, Length=450, Percent_Identity=28.8888888888889, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17538894, Length=307, Percent_Identity=30.2931596091205, Blast_Score=127, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6320352, Length=419, Percent_Identity=45.8233890214797, Blast_Score=331, Evalue=1e-91,
Organism=Saccharomyces cerevisiae, GI6324258, Length=452, Percent_Identity=25.8849557522124, Blast_Score=134, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24645909, Length=224, Percent_Identity=51.3392857142857, Blast_Score=256, Evalue=3e-68,
Organism=Drosophila melanogaster, GI18859875, Length=431, Percent_Identity=27.1461716937355, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI20129315, Length=231, Percent_Identity=30.3030303030303, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24582497, Length=231, Percent_Identity=30.3030303030303, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 44330; Mature: 44198

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVEIKVPEMGESITEATIANWVKKEGDAVKQDEILLELETDKATMEVPAPSSGVLQKIH
CCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCEEEECCCCCHHHHHHHH
KKAGDTVKVKEVVGLIDSAATVSAPAPSSSSPAITTQTNQTSGNDRFNDTLPPAVRKLID
HHCCCCEEHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHC
DNGLNVTAISGSGKNGQITKEDVLKAIESKTSAGVGTATAAKAASSPEIPKAVPVAKRTD
CCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCHHHCC
LPRENAVPMTRLRKVIAERLVSAQHNAAILTTFNEVDMSAVMELRNRYKDRFKEAHNVGL
CCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCH
GFMSFFTKAAIHALKTIPAINAEIRGNDVVYKNFYDIGVAVGGPKGLVVPIVRDADLLSF
HHHHHHHHHHHHHHHHCCCCCCEECCCEEEEEEHEEEEEEECCCCCEEEEEECCCCHHHH
AGVEQEIVRLANRVKDGKIELSEMEGGTFTISNGGIYGSMMSTPILNPPQSGILGLHNIV
CCHHHHHHHHHHHCCCCCEEEEECCCCEEEEECCCEEEHHHCCCCCCCCCCCHHHHHHHH
KRAVVVNDQIVIRPMMYLALSYDHRIVDGKEAVTFLVKVKEAIEDPARLLLEL
HHHHHCCCCHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHCCHHHHHHCC
>Mature Secondary Structure 
SVEIKVPEMGESITEATIANWVKKEGDAVKQDEILLELETDKATMEVPAPSSGVLQKIH
CEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCEEEECCCCCHHHHHHHH
KKAGDTVKVKEVVGLIDSAATVSAPAPSSSSPAITTQTNQTSGNDRFNDTLPPAVRKLID
HHCCCCEEHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHC
DNGLNVTAISGSGKNGQITKEDVLKAIESKTSAGVGTATAAKAASSPEIPKAVPVAKRTD
CCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCHHHCC
LPRENAVPMTRLRKVIAERLVSAQHNAAILTTFNEVDMSAVMELRNRYKDRFKEAHNVGL
CCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCH
GFMSFFTKAAIHALKTIPAINAEIRGNDVVYKNFYDIGVAVGGPKGLVVPIVRDADLLSF
HHHHHHHHHHHHHHHHCCCCCCEECCCEEEEEEHEEEEEEECCCCCEEEEEECCCCHHHH
AGVEQEIVRLANRVKDGKIELSEMEGGTFTISNGGIYGSMMSTPILNPPQSGILGLHNIV
CCHHHHHHHHHHHCCCCCEEEEECCCCEEEEECCCEEEHHHCCCCCCCCCCCHHHHHHHH
KRAVVVNDQIVIRPMMYLALSYDHRIVDGKEAVTFLVKVKEAIEDPARLLLEL
HHHHHCCCCHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12874367 [H]