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The map label for this gene is truB [H]

Identifier: 116328711

GI number: 116328711

Start: 2443038

End: 2443889

Strand: Reverse

Name: truB [H]

Synonym: LBL_2085

Alternate gene names: 116328711

Gene position: 2443889-2443038 (Counterclockwise)

Preceding gene: 116328712

Following gene: 116328710

Centisome position: 67.61

GC content: 41.9

Gene sequence:

>852_bases
ATGACTTCTTCGGATCTTGTAGTGACTGTCCGTAAAAAACTTAAATTCAAAAAGGTCGGTCATACGGGAACCTTGGATCG
GGCGGCAAGCGGTCTTATGATTCTTCCTGTCGGAAGTTGTACTAGCTTTTCTAGCGTATTCTTAGAAAAAGAAAAATCCT
ACCAAGCCTGGGTCAAACTGGGAGAATCTACTGATTCCGGAGATAAGGAAGGTGAAATCTTAGAATGGCTACCGAAAGAA
CAAACGGAAATCTGGTTTCGGAAACATCGAGAGAAACTCAAAGATTTATTCGAACAAGTTCCGAGCTGGGAAACTCAAGA
AGCTCCGGAGGTTTCTGCGCTCAAGGTAAACGGAAGGAGAAGATCGGATCTCTTTCGAGAAGGGGTCGCACTGGTTCCCG
CCGTGCGAAAGATTCGAGTGTATCAGTATGAACTCGGAATATTTTCTCCCGAATTGATTTCTTTTGAGATTCGAGTTTCT
GCGGGGACTTACATCCGTAAGATTGTAATGGATATTTCCGATCGTGTCGGTTTTCCGCTTCGTTTGGAAAAATTGGTCCG
CACGAGTATTGGCAAATTGAATTTGAACCAAGCCGATTCGTATGAAAATCTATTGGAAGGGAAAGTTAAGATTCATTCTC
CGGAATCGATTTTGGAATTTCCCATTGTGGAAATCCCGAGCACAGAAGTGAAGAATGTGTTCAACGGGAGAAAAATAAAA
TTGGAATGGATTCCCGGGAATGAATTTCTTTTAACTTCATCTGAAGGCGAGGTTCTTGCTTGGTGTAGAAAAGAAGAGCA
CGGAGTTCATGAATCGGATTATAAATATTTAAGAGTCTTTCCTAAAAATTAG

Upstream 100 bases:

>100_bases
GAGGAATATATTCAGAGTTTGGATGAATCGCTCAGACTTACCAGAAAACCCACGAGTGCGGACTGAATCCGGATTTTTGC
TCATTCACAAACCGGAAGGG

Downstream 100 bases:

>100_bases
CTTTAAAGAGGCGCCATTGCTTGAAAAATGGTAAAAAGGTATATTCACTCTATGATAGCTGCTGAACAGAAAAAACAAAT
TATCAGTAATTTTGCCCGCA

Product: pseudouridylate synthase, N-terminal subunit

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MTSSDLVVTVRKKLKFKKVGHTGTLDRAASGLMILPVGSCTSFSSVFLEKEKSYQAWVKLGESTDSGDKEGEILEWLPKE
QTEIWFRKHREKLKDLFEQVPSWETQEAPEVSALKVNGRRRSDLFREGVALVPAVRKIRVYQYELGIFSPELISFEIRVS
AGTYIRKIVMDISDRVGFPLRLEKLVRTSIGKLNLNQADSYENLLEGKVKIHSPESILEFPIVEIPSTEVKNVFNGRKIK
LEWIPGNEFLLTSSEGEVLAWCRKEEHGVHESDYKYLRVFPKN

Sequences:

>Translated_283_residues
MTSSDLVVTVRKKLKFKKVGHTGTLDRAASGLMILPVGSCTSFSSVFLEKEKSYQAWVKLGESTDSGDKEGEILEWLPKE
QTEIWFRKHREKLKDLFEQVPSWETQEAPEVSALKVNGRRRSDLFREGVALVPAVRKIRVYQYELGIFSPELISFEIRVS
AGTYIRKIVMDISDRVGFPLRLEKLVRTSIGKLNLNQADSYENLLEGKVKIHSPESILEFPIVEIPSTEVKNVFNGRKIK
LEWIPGNEFLLTSSEGEVLAWCRKEEHGVHESDYKYLRVFPKN
>Mature_282_residues
TSSDLVVTVRKKLKFKKVGHTGTLDRAASGLMILPVGSCTSFSSVFLEKEKSYQAWVKLGESTDSGDKEGEILEWLPKEQ
TEIWFRKHREKLKDLFEQVPSWETQEAPEVSALKVNGRRRSDLFREGVALVPAVRKIRVYQYELGIFSPELISFEIRVSA
GTYIRKIVMDISDRVGFPLRLEKLVRTSIGKLNLNQADSYENLLEGKVKIHSPESILEFPIVEIPSTEVKNVFNGRKIKL
EWIPGNEFLLTSSEGEVLAWCRKEEHGVHESDYKYLRVFPKN

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=191, Percent_Identity=32.4607329842932, Blast_Score=92, Evalue=7e-19,
Organism=Escherichia coli, GI2367200, Length=275, Percent_Identity=27.2727272727273, Blast_Score=105, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6324037, Length=138, Percent_Identity=35.5072463768116, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780 [H]

Pfam domain/function: PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 32433; Mature: 32301

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSDLVVTVRKKLKFKKVGHTGTLDRAASGLMILPVGSCTSFSSVFLEKEKSYQAWVKL
CCCCCEEEEEHHHHHHHCCCCCCCHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHEE
GESTDSGDKEGEILEWLPKEQTEIWFRKHREKLKDLFEQVPSWETQEAPEVSALKVNGRR
CCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCH
RSDLFREGVALVPAVRKIRVYQYELGIFSPELISFEIRVSAGTYIRKIVMDISDRVGFPL
HHHHHHCCHHHHHHHHHEEEEEEECCCCCCCEEEEEEEECCCHHHHHHHHHHHHHCCCCH
RLEKLVRTSIGKLNLNQADSYENLLEGKVKIHSPESILEFPIVEIPSTEVKNVFNGRKIK
HHHHHHHHHCCCCCCCCCCCHHHHHCCCEEEECCHHHHHCCCEECCCHHHHHHCCCCEEE
LEWIPGNEFLLTSSEGEVLAWCRKEEHGVHESDYKYLRVFPKN
EEEECCCEEEEECCCCCEEEEEEHHHCCCCCCCCEEEEEECCC
>Mature Secondary Structure 
TSSDLVVTVRKKLKFKKVGHTGTLDRAASGLMILPVGSCTSFSSVFLEKEKSYQAWVKL
CCCCEEEEEHHHHHHHCCCCCCCHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHEE
GESTDSGDKEGEILEWLPKEQTEIWFRKHREKLKDLFEQVPSWETQEAPEVSALKVNGRR
CCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCH
RSDLFREGVALVPAVRKIRVYQYELGIFSPELISFEIRVSAGTYIRKIVMDISDRVGFPL
HHHHHHCCHHHHHHHHHEEEEEEECCCCCCCEEEEEEEECCCHHHHHHHHHHHHHCCCCH
RLEKLVRTSIGKLNLNQADSYENLLEGKVKIHSPESILEFPIVEIPSTEVKNVFNGRKIK
HHHHHHHHHCCCCCCCCCCCHHHHHCCCEEEECCHHHHHCCCEECCCHHHHHHCCCCEEE
LEWIPGNEFLLTSSEGEVLAWCRKEEHGVHESDYKYLRVFPKN
EEEECCCEEEEECCCCCEEEEEEHHHCCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA