Click here to switch to the map view.

The map label for this gene is purK [H]

Identifier: 116328700

GI number: 116328700

Start: 2428591

End: 2429721

Strand: Direct

Name: purK [H]

Synonym: LBL_2072

Alternate gene names: 116328700

Gene position: 2428591-2429721 (Clockwise)

Preceding gene: 116328699

Following gene: 116328701

Centisome position: 67.19

GC content: 40.32

Gene sequence:

>1131_bases
GTGAAGAATAACGATGTTTTAACTCCAGGTTCGAAGCTTGGGGTAATGGGGGCCGGACAACTCGCGAGAATGTTCTGCTT
GGAAGCAATCCCTTTCGGATACGAGGTTTCCGTTTATTCTCCTGAAAAAAATCCCGCCGCCGCGGGAGTCGGCGCCAAAC
CGTATCTATCCGCATATGAGGACGAAGAAGCGCTGGTCGCTTTTTTAAAAAATATAGATGCTCTTACATTCGAATTTGAG
AATATTCCAGAAATTGCACTTTCTACGATAGATAAATTTTCTAAACAAACCGGACTCAAAGTCCATCCTTCCCCGAATTG
TATTCGAATCGCACAAAATCGATGGAAGGAAAAAAACTCTTTTCGAGAAGCGGGGATCCCAACGGTAAACTTTTATCCGA
TTTTTACGGAAAAAGACAAACTTTCCGTTCTTTCCAACGTGAAATTTCCCTGCATTCTCAAAACGAACACGATGGGTTAC
GATGGAAAAGGACAAATCCAATGTAAGACCAAGAAGGAGCTTTCCTCTGCACTTTCCTCTTTGAAAGAACTCAATCATAT
CGTGGAAGAATTATTTCCGTTTACTGCGGAAGCTTCCGTAATTTTAGCGAGATTCGAAAATGGAAAAATCCTAAACTTTC
GTCCTTCTAGAAACGTTCATAAAAATCACATCTTAGATCTCACATTTTACCCAGGAAATTTTTCGGAAAAGATAGAAACC
GAACTCATAAACCACTCTAAAAAATTGGCGGAAAAGATCGATTATGTGGGAGTTTTCGGAATTGAATTCTTCATCAAAAA
CGAGGAAGTTCTTTGTAACGAATTCGCTCCAAGGCCGCATAACTCTGGGCATTTCAGTCAAAATTCAGGAACCCTTTCCC
AATTTTCTCTTCAATTAAGAACTCTTTGTAATTTACCTTCTCCAAATACGATTCCGACAAAGCCGATTACGATGAAGAAC
ATTCTCGGAGAGGACTACAAACCGAATTCTCCGCTTTGGAACAGTGCATTAGAAAATCCTTATTATCATCTTCATCTTTA
CGGAAAGAACGAAGTTCGAAACGGAAGGAAAATGGGACACTGGAACTATTCCGGTCCTAATCCTGAATCCGCTTTTTCAG
ATTGGAATTAA

Upstream 100 bases:

>100_bases
TAAGAATCCTTTCTCTTCAAGATTCTGAACTTTCCGAAAAACTCGAACAGTATCGAAACCAAATTAGAGAGGATGCTCTT
TCAAAAAACAAGGACCTCTT

Downstream 100 bases:

>100_bases
CTTCCTCAATTCATTACTCGAATATCCCAGACCCTTAGAGCGACTGAAATATAAAATTCATTTTTAGTATTTGGACGTAT
GAAAACCATACCAAGAATTA

Product: phosphoribosylaminoimidazole carboxylase, ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MKNNDVLTPGSKLGVMGAGQLARMFCLEAIPFGYEVSVYSPEKNPAAAGVGAKPYLSAYEDEEALVAFLKNIDALTFEFE
NIPEIALSTIDKFSKQTGLKVHPSPNCIRIAQNRWKEKNSFREAGIPTVNFYPIFTEKDKLSVLSNVKFPCILKTNTMGY
DGKGQIQCKTKKELSSALSSLKELNHIVEELFPFTAEASVILARFENGKILNFRPSRNVHKNHILDLTFYPGNFSEKIET
ELINHSKKLAEKIDYVGVFGIEFFIKNEEVLCNEFAPRPHNSGHFSQNSGTLSQFSLQLRTLCNLPSPNTIPTKPITMKN
ILGEDYKPNSPLWNSALENPYYHLHLYGKNEVRNGRKMGHWNYSGPNPESAFSDWN

Sequences:

>Translated_376_residues
MKNNDVLTPGSKLGVMGAGQLARMFCLEAIPFGYEVSVYSPEKNPAAAGVGAKPYLSAYEDEEALVAFLKNIDALTFEFE
NIPEIALSTIDKFSKQTGLKVHPSPNCIRIAQNRWKEKNSFREAGIPTVNFYPIFTEKDKLSVLSNVKFPCILKTNTMGY
DGKGQIQCKTKKELSSALSSLKELNHIVEELFPFTAEASVILARFENGKILNFRPSRNVHKNHILDLTFYPGNFSEKIET
ELINHSKKLAEKIDYVGVFGIEFFIKNEEVLCNEFAPRPHNSGHFSQNSGTLSQFSLQLRTLCNLPSPNTIPTKPITMKN
ILGEDYKPNSPLWNSALENPYYHLHLYGKNEVRNGRKMGHWNYSGPNPESAFSDWN
>Mature_376_residues
MKNNDVLTPGSKLGVMGAGQLARMFCLEAIPFGYEVSVYSPEKNPAAAGVGAKPYLSAYEDEEALVAFLKNIDALTFEFE
NIPEIALSTIDKFSKQTGLKVHPSPNCIRIAQNRWKEKNSFREAGIPTVNFYPIFTEKDKLSVLSNVKFPCILKTNTMGY
DGKGQIQCKTKKELSSALSSLKELNHIVEELFPFTAEASVILARFENGKILNFRPSRNVHKNHILDLTFYPGNFSEKIET
ELINHSKKLAEKIDYVGVFGIEFFIKNEEVLCNEFAPRPHNSGHFSQNSGTLSQFSLQLRTLCNLPSPNTIPTKPITMKN
ILGEDYKPNSPLWNSALENPYYHLHLYGKNEVRNGRKMGHWNYSGPNPESAFSDWN

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR) [H]

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786733, Length=356, Percent_Identity=29.4943820224719, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI1788155, Length=364, Percent_Identity=22.8021978021978, Blast_Score=88, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6324702, Length=362, Percent_Identity=30.939226519337, Blast_Score=181, Evalue=2e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: =6.3.4.18 [H]

Molecular weight: Translated: 42350; Mature: 42350

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNNDVLTPGSKLGVMGAGQLARMFCLEAIPFGYEVSVYSPEKNPAAAGVGAKPYLSAYE
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCHHCCC
DEEALVAFLKNIDALTFEFENIPEIALSTIDKFSKQTGLKVHPSPNCIRIAQNRWKEKNS
CHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHCCCEECCCCCEEEECHHHHHHHHH
FREAGIPTVNFYPIFTEKDKLSVLSNVKFPCILKTNTMGYDGKGQIQCKTKKELSSALSS
HHHCCCCEEEEEEEEECCHHHHHHHCCCCCEEEEECCCCCCCCCEEEECHHHHHHHHHHH
LKELNHIVEELFPFTAEASVILARFENGKILNFRPSRNVHKNHILDLTFYPGNFSEKIET
HHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEECCCCCCCCCEEEEEEECCCCCHHHHHH
ELINHSKKLAEKIDYVGVFGIEFFIKNEEVLCNEFAPRPHNSGHFSQNSGTLSQFSLQLR
HHHHHHHHHHHHHCCEEEEEEEEEEECCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHH
TLCNLPSPNTIPTKPITMKNILGEDYKPNSPLWNSALENPYYHLHLYGKNEVRNGRKMGH
HHHCCCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHCCCEEEEEEEECHHHCCCCCCCC
WNYSGPNPESAFSDWN
CCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKNNDVLTPGSKLGVMGAGQLARMFCLEAIPFGYEVSVYSPEKNPAAAGVGAKPYLSAYE
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCHHCCC
DEEALVAFLKNIDALTFEFENIPEIALSTIDKFSKQTGLKVHPSPNCIRIAQNRWKEKNS
CHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHCCCEECCCCCEEEECHHHHHHHHH
FREAGIPTVNFYPIFTEKDKLSVLSNVKFPCILKTNTMGYDGKGQIQCKTKKELSSALSS
HHHCCCCEEEEEEEEECCHHHHHHHCCCCCEEEEECCCCCCCCCEEEECHHHHHHHHHHH
LKELNHIVEELFPFTAEASVILARFENGKILNFRPSRNVHKNHILDLTFYPGNFSEKIET
HHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEECCCCCCCCCEEEEEEECCCCCHHHHHH
ELINHSKKLAEKIDYVGVFGIEFFIKNEEVLCNEFAPRPHNSGHFSQNSGTLSQFSLQLR
HHHHHHHHHHHHHCCEEEEEEEEEEECCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHH
TLCNLPSPNTIPTKPITMKNILGEDYKPNSPLWNSALENPYYHLHLYGKNEVRNGRKMGH
HHHCCCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHCCCEEEEEEEECHHHCCCCCCCC
WNYSGPNPESAFSDWN
CCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]