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The map label for this gene is tnpA [H]

Identifier: 116328629

GI number: 116328629

Start: 2320473

End: 2321153

Strand: Direct

Name: tnpA [H]

Synonym: LBL_1990

Alternate gene names: 116328629

Gene position: 2320473-2321153 (Clockwise)

Preceding gene: 116328628

Following gene: 116328631

Centisome position: 64.2

GC content: 33.92

Gene sequence:

>681_bases
ATGAAAAATTCCAGAGTTAAAGTAACACATCAAATTAAAATCAGAGATGAAAAAGGAATATCGAAAGTTTCGGTCGAAGT
TAAATATCATTATATACGCATACTTCCTCCAATTGGTAAAAAGAAAAAATATCCCGAATTAAACCTTACTGTCATTCATG
CACAAGAAAAAGGGACCCCTAAAAATCGCAAAAGGATTGATTGGAAACTGACAACTAATTTGTCGGTTAAGACTAATTTG
GATGCGATTCAAAAAATACAATGGTATGCTTTACGTTGGAAGATTGAAGTTTTTCATAAAATTTTAAAGTCGGGCTGCAA
AGCGGAAGAATCAAAACTTAGAACAGCGGATAGATTGGTAAACCTGATTTCAATCTATTGTATTTTATCTTGGCGAATCT
TCTGGATGACCATGATGAATCGCTCAACAAACTTTGTTTCTCCAGATATTAGATTGACTGCAACGGAGATTCATTTATTG
GACGAGCTGATAAAAAATAAAAATTGCGATAATAAACAAAAGAAAACTCTTACCAACTATATTACTAAAATCGCAAGACT
TGGCGGCTATCTAGCTCGATCTTCTGATCCTCCCCCGGGTAATATGGTTATATGGAGAGGGTTTTCTCGATTAACTGATA
TTACATTGGGATTTAAACTTGCGAAAAATAATTGTGGGTAA

Upstream 100 bases:

>100_bases
TATGAGCTATTTTACTTAGCGAACCAATTGAAAACGAATTTTCTCGTAAGATCCTGCGGGATCGTCTCGCAATAGACGGG
ATCATACGATCCGTAAAGTA

Downstream 100 bases:

>100_bases
TTGTAAGCTACTCACCCCTATAAAAATAAATACCTAAACGCCAGTTTGCATCCAATAACTTTCAAAACTATTCGTATTTG
ATTTCAATTTTCCTAGAGTA

Product: transposase ISLbp10

Products: NA

Alternate protein names: Tnp [H]

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MKNSRVKVTHQIKIRDEKGISKVSVEVKYHYIRILPPIGKKKKYPELNLTVIHAQEKGTPKNRKRIDWKLTTNLSVKTNL
DAIQKIQWYALRWKIEVFHKILKSGCKAEESKLRTADRLVNLISIYCILSWRIFWMTMMNRSTNFVSPDIRLTATEIHLL
DELIKNKNCDNKQKKTLTNYITKIARLGGYLARSSDPPPGNMVIWRGFSRLTDITLGFKLAKNNCG

Sequences:

>Translated_226_residues
MKNSRVKVTHQIKIRDEKGISKVSVEVKYHYIRILPPIGKKKKYPELNLTVIHAQEKGTPKNRKRIDWKLTTNLSVKTNL
DAIQKIQWYALRWKIEVFHKILKSGCKAEESKLRTADRLVNLISIYCILSWRIFWMTMMNRSTNFVSPDIRLTATEIHLL
DELIKNKNCDNKQKKTLTNYITKIARLGGYLARSSDPPPGNMVIWRGFSRLTDITLGFKLAKNNCG
>Mature_226_residues
MKNSRVKVTHQIKIRDEKGISKVSVEVKYHYIRILPPIGKKKKYPELNLTVIHAQEKGTPKNRKRIDWKLTTNLSVKTNL
DAIQKIQWYALRWKIEVFHKILKSGCKAEESKLRTADRLVNLISIYCILSWRIFWMTMMNRSTNFVSPDIRLTATEIHLL
DELIKNKNCDNKQKKTLTNYITKIARLGGYLARSSDPPPGNMVIWRGFSRLTDITLGFKLAKNNCG

Specific function: Mediates transposition of transposon Tn5 by a 'cut and paste' mechanism. First, the monomeric transposase binds the 19 bp inverted DNA repeats flanking the transposon. Then, dimerization of the DNA-bound transposase creates a synaptic DNA complex. After n

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 11 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR002559
- InterPro:   IPR003201
- InterPro:   IPR014737
- InterPro:   IPR014736
- InterPro:   IPR014735 [H]

Pfam domain/function: PF01609 Transposase_11; PF02281 Transposase_Tn5 [H]

EC number: NA

Molecular weight: Translated: 26297; Mature: 26297

Theoretical pI: Translated: 10.82; Mature: 10.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNSRVKVTHQIKIRDEKGISKVSVEVKYHYIRILPPIGKKKKYPELNLTVIHAQEKGTP
CCCCEEEEEEEEEEECCCCCEEEEEEEEEEEEEEECCCCCCCCCCCCCEEEEEECCCCCC
KNRKRIDWKLTTNLSVKTNLDAIQKIQWYALRWKIEVFHKILKSGCKAEESKLRTADRLV
CCCCEEEEEEEECEEEEECHHHHHHHHHHHEEEHHHHHHHHHHCCCCCHHHHHHHHHHHH
NLISIYCILSWRIFWMTMMNRSTNFVSPDIRLTATEIHLLDELIKNKNCDNKQKKTLTNY
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCHHHHHHHHH
ITKIARLGGYLARSSDPPPGNMVIWRGFSRLTDITLGFKLAKNNCG
HHHHHHHCCHHHCCCCCCCCCEEEECCHHHHHHHHHEEEECCCCCC
>Mature Secondary Structure
MKNSRVKVTHQIKIRDEKGISKVSVEVKYHYIRILPPIGKKKKYPELNLTVIHAQEKGTP
CCCCEEEEEEEEEEECCCCCEEEEEEEEEEEEEEECCCCCCCCCCCCCEEEEEECCCCCC
KNRKRIDWKLTTNLSVKTNLDAIQKIQWYALRWKIEVFHKILKSGCKAEESKLRTADRLV
CCCCEEEEEEEECEEEEECHHHHHHHHHHHEEEHHHHHHHHHHCCCCCHHHHHHHHHHHH
NLISIYCILSWRIFWMTMMNRSTNFVSPDIRLTATEIHLLDELIKNKNCDNKQKKTLTNY
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCHHHHHHHHH
ITKIARLGGYLARSSDPPPGNMVIWRGFSRLTDITLGFKLAKNNCG
HHHHHHHCCHHHCCCCCCCCCEEEECCHHHHHHHHHEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6260374; 6271452; 7867940; 6291786; 10884228; 12367522; 11896402 [H]