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The map label for this gene is miaA

Identifier: 116328565

GI number: 116328565

Start: 2242040

End: 2242918

Strand: Reverse

Name: miaA

Synonym: LBL_1922

Alternate gene names: 116328565

Gene position: 2242918-2242040 (Counterclockwise)

Preceding gene: 116328566

Following gene: 116328564

Centisome position: 62.05

GC content: 42.89

Gene sequence:

>879_bases
TTGATTCTTGCCGCTCCGACCGGAGCGGGTAAAACTTCTCTTGTCACCGAACTTGATCCGACCAGGTTCGAAATTCTTTC
CTTCGATTCCAGACAGATCTACAAAAATATGTCGATCGGAACCGCGGCTCCTACTAAAAAAGAGCAGTCTAAGATCGCTC
ATCACCTTGTGGAAGTTCTTTCTCCTTCCGAAGCCGTAGATGCGGGGCTTTATAATCGTCTCGCGGAAGAGGCTCTTCAA
AAAGTTTTGAACCTGGATAAAATTCCGGTTTTTACCGCGGGTACGGGTTTTTATCTCAAAGCGTTTTTGTTCGGAATGTT
TCCCGTTCCGGAAATAGACGTTTCTGTGCGAGATCGAGTTCTTTCTATGAGCAAAGAGGAAAAAAAAAATCTTCTGAAAG
AATTGGATCCGAATGCTTTGGATAAAATATTTCCAGAGGACGATTATAGATTGGGGAGGGCTTTGGAAGTCAATCTTATG
GGAGAGAAGTGGTCCCGTTTGAAAATCGATCCGAATACTTCTGCAATTTGCCGGTACGATTTGGATATTCGTTTAGGTGT
TTTTTTGGATCTGGATCGGAAAGAACTTTATGAAAGAATCAATCTAAGAGCCAAGCAAATGATCGAAAAAGGAATGGCGG
ATGAGGCTTGGAAGATTCAAGAGAGATTCGGAGAGACTTGTCCCGGGCTTAAGTCTTTGGGTTATAATTTTGCACTTGAA
AATAAAAAAGGAAACTCCAATCTAGAGACATTCCTTGCAGATTTAAGTCGGTCTCATAGGAATTACGCCAAAAGGCAGGT
CACTTGGTTTCGTAAGGAACCATACGTTCAACCGATGGGACGGTCCGAGGCGTTGGAACGAATCAAACATATGAAGTAA

Upstream 100 bases:

>100_bases
GTGGATGAGGAAATGGAATTCACCGAGGAACCTCTCGAAGAAGCTTTGGATGAGGACGGTGACGATGCGGAAGAGCCGGA
AGAGTAAGGCTTGTCCGATT

Downstream 100 bases:

>100_bases
CGGAAACAAAAAAAGATGTCTGCTAAAAACAATATACAGGACCAACTCTTAAACACTGCCCGAAAGGATAAATTGGATCT
TACGATTTATCTTCTAAATG

Product: tRNA isopentenyltransferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MILAAPTGAGKTSLVTELDPTRFEILSFDSRQIYKNMSIGTAAPTKKEQSKIAHHLVEVLSPSEAVDAGLYNRLAEEALQ
KVLNLDKIPVFTAGTGFYLKAFLFGMFPVPEIDVSVRDRVLSMSKEEKKNLLKELDPNALDKIFPEDDYRLGRALEVNLM
GEKWSRLKIDPNTSAICRYDLDIRLGVFLDLDRKELYERINLRAKQMIEKGMADEAWKIQERFGETCPGLKSLGYNFALE
NKKGNSNLETFLADLSRSHRNYAKRQVTWFRKEPYVQPMGRSEALERIKHMK

Sequences:

>Translated_292_residues
MILAAPTGAGKTSLVTELDPTRFEILSFDSRQIYKNMSIGTAAPTKKEQSKIAHHLVEVLSPSEAVDAGLYNRLAEEALQ
KVLNLDKIPVFTAGTGFYLKAFLFGMFPVPEIDVSVRDRVLSMSKEEKKNLLKELDPNALDKIFPEDDYRLGRALEVNLM
GEKWSRLKIDPNTSAICRYDLDIRLGVFLDLDRKELYERINLRAKQMIEKGMADEAWKIQERFGETCPGLKSLGYNFALE
NKKGNSNLETFLADLSRSHRNYAKRQVTWFRKEPYVQPMGRSEALERIKHMK
>Mature_292_residues
MILAAPTGAGKTSLVTELDPTRFEILSFDSRQIYKNMSIGTAAPTKKEQSKIAHHLVEVLSPSEAVDAGLYNRLAEEALQ
KVLNLDKIPVFTAGTGFYLKAFLFGMFPVPEIDVSVRDRVLSMSKEEKKNLLKELDPNALDKIFPEDDYRLGRALEVNLM
GEKWSRLKIDPNTSAICRYDLDIRLGVFLDLDRKELYERINLRAKQMIEKGMADEAWKIQERFGETCPGLKSLGYNFALE
NKKGNSNLETFLADLSRSHRNYAKRQVTWFRKEPYVQPMGRSEALERIKHMK

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=321, Percent_Identity=23.6760124610592, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1790613, Length=278, Percent_Identity=30.9352517985612, Blast_Score=124, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI25144712, Length=265, Percent_Identity=26.4150943396226, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6324848, Length=297, Percent_Identity=25.9259259259259, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24649830, Length=234, Percent_Identity=27.3504273504274, Blast_Score=82, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_LEPBJ (Q04S66)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_801012.1
- ProteinModelPortal:   Q04S66
- SMR:   Q04S66
- STRING:   Q04S66
- GeneID:   4410811
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_1703
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   CLSK573952
- BioCyc:   LBOR355277:LBJ_1703-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 33394; Mature: 33394

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILAAPTGAGKTSLVTELDPTRFEILSFDSRQIYKNMSIGTAAPTKKEQSKIAHHLVEVL
CEEECCCCCCCCCEEECCCCCEEEEEECCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
SPSEAVDAGLYNRLAEEALQKVLNLDKIPVFTAGTGFYLKAFLFGMFPVPEIDVSVRDRV
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHCCCCCCCCHHHHHHH
LSMSKEEKKNLLKELDPNALDKIFPEDDYRLGRALEVNLMGEKWSRLKIDPNTSAICRYD
HHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCEEEEEEECCCCCEEEECCCCCEEEEEE
LDIRLGVFLDLDRKELYERINLRAKQMIEKGMADEAWKIQERFGETCPGLKSLGYNFALE
CEEEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHCCCCEEEE
NKKGNSNLETFLADLSRSHRNYAKRQVTWFRKEPYVQPMGRSEALERIKHMK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MILAAPTGAGKTSLVTELDPTRFEILSFDSRQIYKNMSIGTAAPTKKEQSKIAHHLVEVL
CEEECCCCCCCCCEEECCCCCEEEEEECCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
SPSEAVDAGLYNRLAEEALQKVLNLDKIPVFTAGTGFYLKAFLFGMFPVPEIDVSVRDRV
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHCCCCCCCCHHHHHHH
LSMSKEEKKNLLKELDPNALDKIFPEDDYRLGRALEVNLMGEKWSRLKIDPNTSAICRYD
HHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCEEEEEEECCCCCEEEECCCCCEEEEEE
LDIRLGVFLDLDRKELYERINLRAKQMIEKGMADEAWKIQERFGETCPGLKSLGYNFALE
CEEEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHCCCCEEEE
NKKGNSNLETFLADLSRSHRNYAKRQVTWFRKEPYVQPMGRSEALERIKHMK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA