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The map label for this gene is cysS

Identifier: 116328522

GI number: 116328522

Start: 2181318

End: 2182742

Strand: Direct

Name: cysS

Synonym: LBL_1872

Alternate gene names: 116328522

Gene position: 2181318-2182742 (Clockwise)

Preceding gene: 116328521

Following gene: 116328523

Centisome position: 60.35

GC content: 44.21

Gene sequence:

>1425_bases
ATGATAGAGATCCAGTTCTACAATTCCCTTTCGGGTAAAAAAGAAAAATTCTCCCCCGCAAATCCGCACCGAGTGACTGT
TTATTCCTGCGGTCCCACGGTTTATAATTTCGCACATATCGGAAATCTACGCGCGTTTCTTTTCGTAGACGTTTTGCGCA
GATCCCTCAAACTTTTGGGATACGGCGTGGATATGACGATGAACATCACGGATATCGACGACAAGATCATCCGAGATTCC
ATCGCCTCCCAAAAAAGCATCCAAGAATTTACGACCCCTTGGACGGAAGCCTTCTTCGAAGATTTAAAAACCGTTTCCGC
CGAATTTTTAGAACACTACCCCAAAGCCACGGAATCGATTCCGGAGATGATCCAGATCATCCAAAGATTGCAGAACCAAG
GACTCGTTTATGAAAAAGACGAGAACCTTTATTTCTCCATCCAAAAGTTCGAAGGTTACGGAAAACTCAGCAAGATTGAT
ACGTCCGGAATGAAAACCGGAACCCGCTACGACACGGACGAATACGATAAAGAAGATGTGAGAGACTTCGTTCTTTGGAA
AAGCCCGAAACGGAAAGGGGAAGCCTCCTGGAAAACGTCAGTCGGCACCGGCAGACCAGGGTGGCATTTGGAATGTTCCG
CGATGATCCGCAAGATTTACGGAAGCGGCGTGGACATTCATACGGGCGGAGTCGACCTTTTGTTTCCACACCACGAGAAC
GAAATTGCACAGTCAGAAGGGGCCTTTCCTGAAGAATCCTTTGTGAGAACCTGGCTTCACTCCGAGCATCTGCTGGTAGA
AGGACAAAAGATGTCCAAGAGCAAGGGAAATTTTTATACGTTACGCGATCTTGTAGGTCAGGGTTTGGATCCGAAAGCAA
TTCGCTTTCTTTTGATTTCGGCCCATTATCGTTCTAAGCTGAACTTTTCTACGGATCGAATCGCGGAAGCCTCGGCGAAT
ATTAGAAAAATCCAAAATTGCTTGGACCGTCTTTTGGACATAGCACAGAACGTAAAAATAGACCCGTCCTATTCGTTTAA
TGACGAACTTACCCTAAGATGGAAAAAGGAATTTGAAGAATCCTTGGCCGACGATTTAAACGTTTCTAAAGCTTTAGCCG
TAGTTTTCGAATCTTTAAAAACGATAAACGCCTTCCTTGATTCGAAAAAAAATCGAGTTGCGGAAATTTCAGCGAATGAA
TTCATTCAAATTCTTGCATATTATGATCGTATATTCGGAGTCCTAAACTTCGAATCCCAAAAAGATCCTCTCATCGATTC
GGAAATCGATTCCCTTATCCAAGAAAGACAAACTGCAAGAAAGAATAAGGACTTTGCACGTTCCGACGCGATTCGAGATC
AACTTTTAGCTCAGGGCATTCTGATCGAAGACACGAAGGATGGAATCCGTTGGAGGAGAAAATAA

Upstream 100 bases:

>100_bases
GGATGCAAGTTTATCCCACAGAAGGAAACGAAGCCGGTCTAAAAGACGATAAACAAAACGGAGCTAATTTAGAATTTGTG
AGGGAAATTTTCTTTCCCGA

Downstream 100 bases:

>100_bases
CCAGATCGGAATACATCTTTGGAAAACGGACTCTTATCGAACTTACGGAGGCCCATATTGGAAAAGAACATTCTTTTCCT
TTTACCGATTTGTATGTTAA

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MIEIQFYNSLSGKKEKFSPANPHRVTVYSCGPTVYNFAHIGNLRAFLFVDVLRRSLKLLGYGVDMTMNITDIDDKIIRDS
IASQKSIQEFTTPWTEAFFEDLKTVSAEFLEHYPKATESIPEMIQIIQRLQNQGLVYEKDENLYFSIQKFEGYGKLSKID
TSGMKTGTRYDTDEYDKEDVRDFVLWKSPKRKGEASWKTSVGTGRPGWHLECSAMIRKIYGSGVDIHTGGVDLLFPHHEN
EIAQSEGAFPEESFVRTWLHSEHLLVEGQKMSKSKGNFYTLRDLVGQGLDPKAIRFLLISAHYRSKLNFSTDRIAEASAN
IRKIQNCLDRLLDIAQNVKIDPSYSFNDELTLRWKKEFEESLADDLNVSKALAVVFESLKTINAFLDSKKNRVAEISANE
FIQILAYYDRIFGVLNFESQKDPLIDSEIDSLIQERQTARKNKDFARSDAIRDQLLAQGILIEDTKDGIRWRRK

Sequences:

>Translated_474_residues
MIEIQFYNSLSGKKEKFSPANPHRVTVYSCGPTVYNFAHIGNLRAFLFVDVLRRSLKLLGYGVDMTMNITDIDDKIIRDS
IASQKSIQEFTTPWTEAFFEDLKTVSAEFLEHYPKATESIPEMIQIIQRLQNQGLVYEKDENLYFSIQKFEGYGKLSKID
TSGMKTGTRYDTDEYDKEDVRDFVLWKSPKRKGEASWKTSVGTGRPGWHLECSAMIRKIYGSGVDIHTGGVDLLFPHHEN
EIAQSEGAFPEESFVRTWLHSEHLLVEGQKMSKSKGNFYTLRDLVGQGLDPKAIRFLLISAHYRSKLNFSTDRIAEASAN
IRKIQNCLDRLLDIAQNVKIDPSYSFNDELTLRWKKEFEESLADDLNVSKALAVVFESLKTINAFLDSKKNRVAEISANE
FIQILAYYDRIFGVLNFESQKDPLIDSEIDSLIQERQTARKNKDFARSDAIRDQLLAQGILIEDTKDGIRWRRK
>Mature_474_residues
MIEIQFYNSLSGKKEKFSPANPHRVTVYSCGPTVYNFAHIGNLRAFLFVDVLRRSLKLLGYGVDMTMNITDIDDKIIRDS
IASQKSIQEFTTPWTEAFFEDLKTVSAEFLEHYPKATESIPEMIQIIQRLQNQGLVYEKDENLYFSIQKFEGYGKLSKID
TSGMKTGTRYDTDEYDKEDVRDFVLWKSPKRKGEASWKTSVGTGRPGWHLECSAMIRKIYGSGVDIHTGGVDLLFPHHEN
EIAQSEGAFPEESFVRTWLHSEHLLVEGQKMSKSKGNFYTLRDLVGQGLDPKAIRFLLISAHYRSKLNFSTDRIAEASAN
IRKIQNCLDRLLDIAQNVKIDPSYSFNDELTLRWKKEFEESLADDLNVSKALAVVFESLKTINAFLDSKKNRVAEISANE
FIQILAYYDRIFGVLNFESQKDPLIDSEIDSLIQERQTARKNKDFARSDAIRDQLLAQGILIEDTKDGIRWRRK

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=484, Percent_Identity=32.2314049586777, Blast_Score=229, Evalue=4e-60,
Organism=Homo sapiens, GI303304987, Length=236, Percent_Identity=34.3220338983051, Blast_Score=152, Evalue=8e-37,
Organism=Homo sapiens, GI62240992, Length=236, Percent_Identity=34.3220338983051, Blast_Score=152, Evalue=9e-37,
Organism=Homo sapiens, GI21269877, Length=236, Percent_Identity=34.3220338983051, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI10835051, Length=236, Percent_Identity=34.3220338983051, Blast_Score=151, Evalue=1e-36,
Organism=Escherichia coli, GI1786737, Length=480, Percent_Identity=37.7083333333333, Blast_Score=282, Evalue=3e-77,
Organism=Caenorhabditis elegans, GI17509795, Length=431, Percent_Identity=28.0742459396752, Blast_Score=146, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17509797, Length=431, Percent_Identity=28.0742459396752, Blast_Score=145, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6324082, Length=374, Percent_Identity=28.8770053475936, Blast_Score=172, Evalue=1e-43,
Organism=Drosophila melanogaster, GI19922218, Length=504, Percent_Identity=29.5634920634921, Blast_Score=209, Evalue=4e-54,
Organism=Drosophila melanogaster, GI19922364, Length=448, Percent_Identity=27.2321428571429, Blast_Score=166, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_LEPBJ (Q04SB0)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_800968.1
- ProteinModelPortal:   Q04SB0
- SMR:   Q04SB0
- STRING:   Q04SB0
- GeneID:   4411044
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_1653
- eggNOG:   COG0215
- HOGENOM:   HBG327651
- OMA:   DFDALNM
- ProtClustDB:   PRK00260
- BioCyc:   LBOR355277:LBJ_1653-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 54455; Mature: 54455

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 273-273

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEIQFYNSLSGKKEKFSPANPHRVTVYSCGPTVYNFAHIGNLRAFLFVDVLRRSLKLLG
CEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHHHHC
YGVDMTMNITDIDDKIIRDSIASQKSIQEFTTPWTEAFFEDLKTVSAEFLEHYPKATESI
CCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHH
PEMIQIIQRLQNQGLVYEKDENLYFSIQKFEGYGKLSKIDTSGMKTGTRYDTDEYDKEDV
HHHHHHHHHHHHCCCEEEECCCEEEEEEEECCCCCCHHCCCCCCCCCCCCCCCCCCHHHH
RDFVLWKSPKRKGEASWKTSVGTGRPGWHLECSAMIRKIYGSGVDIHTGGVDLLFPHHEN
HHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHCCCCEEECCCEEEEECCCCC
EIAQSEGAFPEESFVRTWLHSEHLLVEGQKMSKSKGNFYTLRDLVGQGLDPKAIRFLLIS
HHHHHCCCCCHHHHHHHHHCCCCEEEECCHHCCCCCCEEEHHHHHCCCCCHHHHHHHHHH
AHYRSKLNFSTDRIAEASANIRKIQNCLDRLLDIAQNVKIDPSYSFNDELTLRWKKEFEE
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEHHHHHH
SLADDLNVSKALAVVFESLKTINAFLDSKKNRVAEISANEFIQILAYYDRIFGVLNFESQ
HHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHCCCCC
KDPLIDSEIDSLIQERQTARKNKDFARSDAIRDQLLAQGILIEDTKDGIRWRRK
CCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCC
>Mature Secondary Structure
MIEIQFYNSLSGKKEKFSPANPHRVTVYSCGPTVYNFAHIGNLRAFLFVDVLRRSLKLLG
CEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHHHHC
YGVDMTMNITDIDDKIIRDSIASQKSIQEFTTPWTEAFFEDLKTVSAEFLEHYPKATESI
CCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHH
PEMIQIIQRLQNQGLVYEKDENLYFSIQKFEGYGKLSKIDTSGMKTGTRYDTDEYDKEDV
HHHHHHHHHHHHCCCEEEECCCEEEEEEEECCCCCCHHCCCCCCCCCCCCCCCCCCHHHH
RDFVLWKSPKRKGEASWKTSVGTGRPGWHLECSAMIRKIYGSGVDIHTGGVDLLFPHHEN
HHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHCCCCEEECCCEEEEECCCCC
EIAQSEGAFPEESFVRTWLHSEHLLVEGQKMSKSKGNFYTLRDLVGQGLDPKAIRFLLIS
HHHHHCCCCCHHHHHHHHHCCCCEEEECCHHCCCCCCEEEHHHHHCCCCCHHHHHHHHHH
AHYRSKLNFSTDRIAEASANIRKIQNCLDRLLDIAQNVKIDPSYSFNDELTLRWKKEFEE
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEHHHHHH
SLADDLNVSKALAVVFESLKTINAFLDSKKNRVAEISANEFIQILAYYDRIFGVLNFESQ
HHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHCCCCC
KDPLIDSEIDSLIQERQTARKNKDFARSDAIRDQLLAQGILIEDTKDGIRWRRK
CCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA