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The map label for this gene is spoU [C]

Identifier: 116328523

GI number: 116328523

Start: 2182727

End: 2183488

Strand: Direct

Name: spoU [C]

Synonym: LBL_1873

Alternate gene names: 116328523

Gene position: 2182727-2183488 (Clockwise)

Preceding gene: 116328522

Following gene: 116328524

Centisome position: 60.39

GC content: 42.91

Gene sequence:

>762_bases
TTGGAGGAGAAAATAACCAGATCGGAATACATCTTTGGAAAACGGACTCTTATCGAACTTACGGAGGCCCATATTGGAAA
AGAACATTCTTTTCCTTTTACCGATTTGTATGTTAAAGAGAATCCGGGAACGGATATCGTCGAAAAAATATTAAACCGAC
TTCCTTCTTTCGTAAAAGTTCATAGGATTTCCGGTTCGAAACTGGACTCGCTCGTTCCGGGAAGAAATCACCAAGGAGTA
GTCGCTCTCAAAAGTCCTTTTAGACAACAAATTTCCGACAAAAAAAATCTGGAAGAATACCTCTCTGAAAAACCGGGGAC
CTTTCTCGTTTTGGATCGAATCCAAGATCCGGGGAACTTGGGAAATATTCTGAGAACCGCAGAGTGTTTCGGAGTTACGA
ACATCATTCTCCCCGAAAGGGAATCGGCGGGAATCACTCCCGTAGTAGAGAAGGTTTCTTCGGGCGCACTTTCGTTCCTA
AAAATTTTCACGGTCAAAAATCTTGCAAACACTCTGGAACTCTTAAAAGAAAACGGCTATTGGATTGTGTCCACAAGCGA
CCGAGGAACGGAAAACTGGTCCAAACTTCCGGAACTCAAAGAGCTCGCAATTCTCATGGGTAACGAAGGAGAAGGGGTCA
AAAGAATCCTGTTGGAAAAATCGGACTTTGTCCTTCGAATTCCCATACACGGAAATCTTTCTTCCTTAAACGTCACTGTT
GCAACCGGAATCGTCTTGGACAGAATTGTAAATCGTAAATGA

Upstream 100 bases:

>100_bases
AAACTGCAAGAAAGAATAAGGACTTTGCACGTTCCGACGCGATTCGAGATCAACTTTTAGCTCAGGGCATTCTGATCGAA
GACACGAAGGATGGAATCCG

Downstream 100 bases:

>100_bases
AATATTCGATTTTTTGAAACTTCGTTTCCCGTTTTGCTTTCGTAATCTTTTGCAATCATCGTTTAATTTTAAGAATTATC
TCTTGCATTTTTCGATCTTT

Product: tRNA (guanosine-2'-O-)-methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MEEKITRSEYIFGKRTLIELTEAHIGKEHSFPFTDLYVKENPGTDIVEKILNRLPSFVKVHRISGSKLDSLVPGRNHQGV
VALKSPFRQQISDKKNLEEYLSEKPGTFLVLDRIQDPGNLGNILRTAECFGVTNIILPERESAGITPVVEKVSSGALSFL
KIFTVKNLANTLELLKENGYWIVSTSDRGTENWSKLPELKELAILMGNEGEGVKRILLEKSDFVLRIPIHGNLSSLNVTV
ATGIVLDRIVNRK

Sequences:

>Translated_253_residues
MEEKITRSEYIFGKRTLIELTEAHIGKEHSFPFTDLYVKENPGTDIVEKILNRLPSFVKVHRISGSKLDSLVPGRNHQGV
VALKSPFRQQISDKKNLEEYLSEKPGTFLVLDRIQDPGNLGNILRTAECFGVTNIILPERESAGITPVVEKVSSGALSFL
KIFTVKNLANTLELLKENGYWIVSTSDRGTENWSKLPELKELAILMGNEGEGVKRILLEKSDFVLRIPIHGNLSSLNVTV
ATGIVLDRIVNRK
>Mature_253_residues
MEEKITRSEYIFGKRTLIELTEAHIGKEHSFPFTDLYVKENPGTDIVEKILNRLPSFVKVHRISGSKLDSLVPGRNHQGV
VALKSPFRQQISDKKNLEEYLSEKPGTFLVLDRIQDPGNLGNILRTAECFGVTNIILPERESAGITPVVEKVSSGALSFL
KIFTVKNLANTLELLKENGYWIVSTSDRGTENWSKLPELKELAILMGNEGEGVKRILLEKSDFVLRIPIHGNLSSLNVTV
ATGIVLDRIVNRK

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=216, Percent_Identity=32.4074074074074, Blast_Score=95, Evalue=7e-20,
Organism=Escherichia coli, GI1790623, Length=187, Percent_Identity=38.5026737967914, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1788935, Length=140, Percent_Identity=26.4285714285714, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17555908, Length=262, Percent_Identity=28.2442748091603, Blast_Score=78, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6324775, Length=169, Percent_Identity=34.9112426035503, Blast_Score=85, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 28305; Mature: 28305

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEKITRSEYIFGKRTLIELTEAHIGKEHSFPFTDLYVKENPGTDIVEKILNRLPSFVKV
CCCCCCHHHHHHCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHCHHHHEE
HRISGSKLDSLVPGRNHQGVVALKSPFRQQISDKKNLEEYLSEKPGTFLVLDRIQDPGNL
HHCCCCHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCH
GNILRTAECFGVTNIILPERESAGITPVVEKVSSGALSFLKIFTVKNLANTLELLKENGY
HHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCE
WIVSTSDRGTENWSKLPELKELAILMGNEGEGVKRILLEKSDFVLRIPIHGNLSSLNVTV
EEEECCCCCCCHHHHCHHHHHHHEEECCCCCHHHHHHCCCCCCEEEEEECCCCCCEEEEH
ATGIVLDRIVNRK
HHHHHHHHHHCCC
>Mature Secondary Structure
MEEKITRSEYIFGKRTLIELTEAHIGKEHSFPFTDLYVKENPGTDIVEKILNRLPSFVKV
CCCCCCHHHHHHCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHCHHHHEE
HRISGSKLDSLVPGRNHQGVVALKSPFRQQISDKKNLEEYLSEKPGTFLVLDRIQDPGNL
HHCCCCHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCH
GNILRTAECFGVTNIILPERESAGITPVVEKVSSGALSFLKIFTVKNLANTLELLKENGY
HHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCE
WIVSTSDRGTENWSKLPELKELAILMGNEGEGVKRILLEKSDFVLRIPIHGNLSSLNVTV
EEEECCCCCCCHHHHCHHHHHHHEEECCCCCHHHHHHCCCCCCEEEEEECCCCCCEEEEH
ATGIVLDRIVNRK
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA