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The map label for this gene is trmD

Identifier: 116328320

GI number: 116328320

Start: 1937765

End: 1938433

Strand: Reverse

Name: trmD

Synonym: LBL_1657

Alternate gene names: 116328320

Gene position: 1938433-1937765 (Counterclockwise)

Preceding gene: 116328321

Following gene: 116328319

Centisome position: 53.63

GC content: 42.75

Gene sequence:

>669_bases
ATGAAGTTTAATTTCATTACACTTTTTCCTGAAAAGATCCAATCTTATTTCTCGGAGGGACTGCAGCAAAAGGCGATTGA
GTCCGGGATATTTTCTATAAACACGATCCAACTCAGGGATTTTTCGGGAAACAAACACAATCGAGTGGATGATACGATTT
ACGGCGGGGGACCGGGGATGCTTCTTCGAGTGGAACCGATTCACAAGGCACTTTTATCTTTGGGAGAGAATAAAGGAATT
GTTATTTTAACTTCTCCGTCGGGAATTCCGTTTCATCAAGGCATCGCCAACAAATTGAAAGAAACCGGAAAACCTCTAAC
ATTCATTTCCGGTTATTACGAAGGTGTGGATCACCGTGTTGCGGAACATCTAGTTGACATGGAAATGTCTCTTGGAAATT
ATGTAATATCTGCCGGGGATTTAGCCAGTATTTGTATAGCAGATGCTGTGTCCAGGCTTTTGCCTGGTTTTTTAGGGGCA
GGGGAAAGCCTTCTGGATGAGTCTCATAATTATCCGGACGTTTTGGAATATCCGCAGTTCACGAAACCCTCGGAATACAA
TGGATGGAAAGTTCCTGAAGTGCTACTTAGCGGCAATCACGCTTCGATTTTAGCGTGGAGGGAACAAAATAGAAAAAAGA
TCGATCCCGATCAAGAGAGGAAATTATGA

Upstream 100 bases:

>100_bases
TCAGAAGAGGAGGAAATTCTCATTCCTTTTTTGCATGTTTTTGTGGGGGAACTGAATCTGGAAAAAAAGACGATCGTATT
GATACAACCGGAGCTTTGGA

Downstream 100 bases:

>100_bases
ATCAACTTTTAAGAAAAGTATTAACTTCGGACGCTGAAAGAAAACAGAATTTCGCGGTAGGGGATACTGTTAAAGTACAT
TATAAAATTGTTGAATCCGG

Product: tRNA (guanine-N(1)-)-methyltransferase/unknown domain fusion protein

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MKFNFITLFPEKIQSYFSEGLQQKAIESGIFSINTIQLRDFSGNKHNRVDDTIYGGGPGMLLRVEPIHKALLSLGENKGI
VILTSPSGIPFHQGIANKLKETGKPLTFISGYYEGVDHRVAEHLVDMEMSLGNYVISAGDLASICIADAVSRLLPGFLGA
GESLLDESHNYPDVLEYPQFTKPSEYNGWKVPEVLLSGNHASILAWREQNRKKIDPDQERKL

Sequences:

>Translated_222_residues
MKFNFITLFPEKIQSYFSEGLQQKAIESGIFSINTIQLRDFSGNKHNRVDDTIYGGGPGMLLRVEPIHKALLSLGENKGI
VILTSPSGIPFHQGIANKLKETGKPLTFISGYYEGVDHRVAEHLVDMEMSLGNYVISAGDLASICIADAVSRLLPGFLGA
GESLLDESHNYPDVLEYPQFTKPSEYNGWKVPEVLLSGNHASILAWREQNRKKIDPDQERKL
>Mature_222_residues
MKFNFITLFPEKIQSYFSEGLQQKAIESGIFSINTIQLRDFSGNKHNRVDDTIYGGGPGMLLRVEPIHKALLSLGENKGI
VILTSPSGIPFHQGIANKLKETGKPLTFISGYYEGVDHRVAEHLVDMEMSLGNYVISAGDLASICIADAVSRLLPGFLGA
GESLLDESHNYPDVLEYPQFTKPSEYNGWKVPEVLLSGNHASILAWREQNRKKIDPDQERKL

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=210, Percent_Identity=39.0476190476191, Blast_Score=150, Evalue=6e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_LEPBJ (Q04SW4)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_800764.1
- ProteinModelPortal:   Q04SW4
- SMR:   Q04SW4
- STRING:   Q04SW4
- GeneID:   4410046
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_1433
- eggNOG:   COG0336
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- ProtClustDB:   PRK14599
- BioCyc:   LBOR355277:LBJ_1433-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 24675; Mature: 24675

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: BINDING 111-111

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFNFITLFPEKIQSYFSEGLQQKAIESGIFSINTIQLRDFSGNKHNRVDDTIYGGGPGM
CCCEEEEECHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCCCCEECCCCCE
LLRVEPIHKALLSLGENKGIVILTSPSGIPFHQGIANKLKETGKPLTFISGYYEGVDHRV
EEEECHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHH
AEHLVDMEMSLGNYVISAGDLASICIADAVSRLLPGFLGAGESLLDESHNYPDVLEYPQF
HHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHCCCCC
TKPSEYNGWKVPEVLLSGNHASILAWREQNRKKIDPDQERKL
CCCCCCCCCCCCHHEECCCCEEEEEEECCCCCCCCCHHHCCC
>Mature Secondary Structure
MKFNFITLFPEKIQSYFSEGLQQKAIESGIFSINTIQLRDFSGNKHNRVDDTIYGGGPGM
CCCEEEEECHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCCCCEECCCCCE
LLRVEPIHKALLSLGENKGIVILTSPSGIPFHQGIANKLKETGKPLTFISGYYEGVDHRV
EEEECHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHH
AEHLVDMEMSLGNYVISAGDLASICIADAVSRLLPGFLGAGESLLDESHNYPDVLEYPQF
HHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHCCCCC
TKPSEYNGWKVPEVLLSGNHASILAWREQNRKKIDPDQERKL
CCCCCCCCCCCCHHEECCCCEEEEEEECCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA