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The map label for this gene is gspE [H]

Identifier: 116328308

GI number: 116328308

Start: 1926040

End: 1927713

Strand: Reverse

Name: gspE [H]

Synonym: LBL_1644

Alternate gene names: 116328308

Gene position: 1927713-1926040 (Counterclockwise)

Preceding gene: 116328309

Following gene: 116328307

Centisome position: 53.33

GC content: 43.79

Gene sequence:

>1674_bases
TTGAAAACTCTCGGAGATATCCTAATCGAAGAGGGGATCATATCCGAAAAAGATCTGGAAGATTCCCTTAAGGTTCAGAA
GAAAAATAACCTTCCGCTCAGTCATATCATTCAGAAAAAAGGAATCGCCGGAGAAACCGATATTCTCCGTGCCTTATCCA
AACTCTATCAGTTTGAATTCAGGGAAAAACTTGAATTTACGGGAATAGAAGACGTATTTTTACAAATTCCGTTGAAACTC
ATTCAAAAAAGCAGGATCGTTCCATTTCAACTTTCCAAAAAGACGATTCGAGTCGCCGTGTCCGATCCGTCCGATCTGCA
TCCGATGGACGACGTGCGGAATTTCTTAAAAGGATATAACGTAGAATTTGTTCTCGCTCCGGAGCCGGAGATCATGAGAA
TCATCCATTCTCAGTTTGATACTACCTCTTCATCAGCAAAAGAAATGCTAAATGAAATGGAGGGAAGTTTTTCGGAACTT
GCGGAAGCTTTTGAAAACGAAACTCTAGATTTAAGCGACGACGCCCCTATCATCAAAATGGTAAATGTGATTCTTTCGCA
GGCCGTCAACGAAAGAGCTTCCGACATTCACATCGAGCCGTATGAAAAGTCACTCGTGGTTCGTTACAGGGTGGATGGTA
TTTTGCATAACGTTCTTAGTCCTCCCAAATCCTATCACGCCGGAATTTCTTCCAGGATCAAGATCATGTCGAATCTGAAT
ATCGCAGAGAATCGACTTCCTCAAGACGGGAGAATTAAGCTCAGACTTGTTGGAAAGGACATCGATATCCGGGTTTCTAC
GATTCCTTGTCAGTTCGGAGAAAGGATCGTTATGAGGCTTTTGAACAAAACGGATCAAAAGTATTCTTTGGATACGATGG
GTTTTTATCCCGATCTCATCAAATCGCTTCGTTCTCTAATCTACGAACCTCATGGAATCGTTCTCGTTACGGGACCGACC
GGATCGGGAAAATCCACCACACTCTATTCGGCGCTCAGCGAACTGAACACAGAAGAAAGAAACATCATCACTTGTGAAGA
TCCCGTAGAGTATCAGATCGAAGGAATTTCTCAGATGCAAATGCAGGAGAAAATCGGTCTTACGTTTGCTACCGGTTTAA
GAGCGATCTTACGACAAGACCCGGATGTGATTATGGTGGGGGAGATTCGAGACGAGGAAACCGCGAGGATCGCGATCCAG
GCTTCTCTTACGGGGCACCTTGTGTTTTCCACATTGCATACGAACGACGCGGCCAGTGCGGCGACTCGTTTGATCGATAT
GGGAATCGAACCGTATCTTATCACTTCCACGGTTTTGGGTTTTATGGCTCAGAGACTTGTACGTGTCATCTGCACTCAAT
GTAAAGAGATTTACAAACCCACCGCTTCGGAATTGGAATCGATTGGAATTTCTAGGAAGGCTTTAAAAAACGGAAGCTTA
CACAGAGGTAAGGGTTGTTCTCATTGTATGGGAACCGGTTTTAAAGGAAGAACGGGAATCTACGAACTTTTGTTAGTGAA
TTCTCACATTAAACATGCGATCCTGCAAGGTAAGGATGCGGGTCAGTTAAACGAGATCGCGCTCGAACATAATTTCCAAA
CTCTAAAAGATTACGGAGTCAAAAAGGTAATCGACGGAGTAACTACGATCGACGAAGTCCTCAGAGTAACCTAA

Upstream 100 bases:

>100_bases
GAAGAAAAAGATGCTACAGGAACAATACGAGCTCGAAAGAGAAAGAATCCTCAATAAAGAAAAAGAAATCAAATCTTTCT
TTGAAAAAGAGGATTAAAAT

Downstream 100 bases:

>100_bases
TTTCATGGCCATTTATTCTTACGTTGCATTCAACAAAAAGGGAAAGGAAGAAAAGGGAATCATAGACGCGGCTTCTCTTC
AGGCTGCGAGATCCAAGTTA

Product: Type II secretory pathway ATPase, protein E

Products: NA

Alternate protein names: Type II traffic warden ATPase [H]

Number of amino acids: Translated: 557; Mature: 557

Protein sequence:

>557_residues
MKTLGDILIEEGIISEKDLEDSLKVQKKNNLPLSHIIQKKGIAGETDILRALSKLYQFEFREKLEFTGIEDVFLQIPLKL
IQKSRIVPFQLSKKTIRVAVSDPSDLHPMDDVRNFLKGYNVEFVLAPEPEIMRIIHSQFDTTSSSAKEMLNEMEGSFSEL
AEAFENETLDLSDDAPIIKMVNVILSQAVNERASDIHIEPYEKSLVVRYRVDGILHNVLSPPKSYHAGISSRIKIMSNLN
IAENRLPQDGRIKLRLVGKDIDIRVSTIPCQFGERIVMRLLNKTDQKYSLDTMGFYPDLIKSLRSLIYEPHGIVLVTGPT
GSGKSTTLYSALSELNTEERNIITCEDPVEYQIEGISQMQMQEKIGLTFATGLRAILRQDPDVIMVGEIRDEETARIAIQ
ASLTGHLVFSTLHTNDAASAATRLIDMGIEPYLITSTVLGFMAQRLVRVICTQCKEIYKPTASELESIGISRKALKNGSL
HRGKGCSHCMGTGFKGRTGIYELLLVNSHIKHAILQGKDAGQLNEIALEHNFQTLKDYGVKKVIDGVTTIDEVLRVT

Sequences:

>Translated_557_residues
MKTLGDILIEEGIISEKDLEDSLKVQKKNNLPLSHIIQKKGIAGETDILRALSKLYQFEFREKLEFTGIEDVFLQIPLKL
IQKSRIVPFQLSKKTIRVAVSDPSDLHPMDDVRNFLKGYNVEFVLAPEPEIMRIIHSQFDTTSSSAKEMLNEMEGSFSEL
AEAFENETLDLSDDAPIIKMVNVILSQAVNERASDIHIEPYEKSLVVRYRVDGILHNVLSPPKSYHAGISSRIKIMSNLN
IAENRLPQDGRIKLRLVGKDIDIRVSTIPCQFGERIVMRLLNKTDQKYSLDTMGFYPDLIKSLRSLIYEPHGIVLVTGPT
GSGKSTTLYSALSELNTEERNIITCEDPVEYQIEGISQMQMQEKIGLTFATGLRAILRQDPDVIMVGEIRDEETARIAIQ
ASLTGHLVFSTLHTNDAASAATRLIDMGIEPYLITSTVLGFMAQRLVRVICTQCKEIYKPTASELESIGISRKALKNGSL
HRGKGCSHCMGTGFKGRTGIYELLLVNSHIKHAILQGKDAGQLNEIALEHNFQTLKDYGVKKVIDGVTTIDEVLRVT
>Mature_557_residues
MKTLGDILIEEGIISEKDLEDSLKVQKKNNLPLSHIIQKKGIAGETDILRALSKLYQFEFREKLEFTGIEDVFLQIPLKL
IQKSRIVPFQLSKKTIRVAVSDPSDLHPMDDVRNFLKGYNVEFVLAPEPEIMRIIHSQFDTTSSSAKEMLNEMEGSFSEL
AEAFENETLDLSDDAPIIKMVNVILSQAVNERASDIHIEPYEKSLVVRYRVDGILHNVLSPPKSYHAGISSRIKIMSNLN
IAENRLPQDGRIKLRLVGKDIDIRVSTIPCQFGERIVMRLLNKTDQKYSLDTMGFYPDLIKSLRSLIYEPHGIVLVTGPT
GSGKSTTLYSALSELNTEERNIITCEDPVEYQIEGISQMQMQEKIGLTFATGLRAILRQDPDVIMVGEIRDEETARIAIQ
ASLTGHLVFSTLHTNDAASAATRLIDMGIEPYLITSTVLGFMAQRLVRVICTQCKEIYKPTASELESIGISRKALKNGSL
HRGKGCSHCMGTGFKGRTGIYELLLVNSHIKHAILQGKDAGQLNEIALEHNFQTLKDYGVKKVIDGVTTIDEVLRVT

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of a variety of enzymes across the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=423, Percent_Identity=48.4633569739953, Blast_Score=404, Evalue=1e-114,
Organism=Escherichia coli, GI1786296, Length=445, Percent_Identity=40.6741573033708, Blast_Score=320, Evalue=2e-88,
Organism=Escherichia coli, GI87082188, Length=169, Percent_Identity=46.1538461538462, Blast_Score=130, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 62296; Mature: 62296

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLGDILIEEGIISEKDLEDSLKVQKKNNLPLSHIIQKKGIAGETDILRALSKLYQFEF
CCCHHHHHHHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHH
REKLEFTGIEDVFLQIPLKLIQKSRIVPFQLSKKTIRVAVSDPSDLHPMDDVRNFLKGYN
HHHHCCCCHHHHHHHHHHHHHHCCCCCCEEECCEEEEEEECCCCCCCCHHHHHHHHCCCC
VEFVLAPEPEIMRIIHSQFDTTSSSAKEMLNEMEGSFSELAEAFENETLDLSDDAPIIKM
EEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCHHHHH
VNVILSQAVNERASDIHIEPYEKSLVVRYRVDGILHNVLSPPKSYHAGISSRIKIMSNLN
HHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHHHHHCCCHHHHCCHHHHHHHHHCCC
IAENRLPQDGRIKLRLVGKDIDIRVSTIPCQFGERIVMRLLNKTDQKYSLDTMGFYPDLI
HHHHCCCCCCCEEEEEECCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
KSLRSLIYEPHGIVLVTGPTGSGKSTTLYSALSELNTEERNIITCEDPVEYQIEGISQMQ
HHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCEEECCCCCHHHHCCHHHHH
MQEKIGLTFATGLRAILRQDPDVIMVGEIRDEETARIAIQASLTGHLVFSTLHTNDAASA
HHHHHCCHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEECCEEEEEEEECCCHHHHH
ATRLIDMGIEPYLITSTVLGFMAQRLVRVICTQCKEIYKPTASELESIGISRKALKNGSL
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHCCCCC
HRGKGCSHCMGTGFKGRTGIYELLLVNSHIKHAILQGKDAGQLNEIALEHNFQTLKDYGV
CCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHCCH
KKVIDGVTTIDEVLRVT
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKTLGDILIEEGIISEKDLEDSLKVQKKNNLPLSHIIQKKGIAGETDILRALSKLYQFEF
CCCHHHHHHHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHH
REKLEFTGIEDVFLQIPLKLIQKSRIVPFQLSKKTIRVAVSDPSDLHPMDDVRNFLKGYN
HHHHCCCCHHHHHHHHHHHHHHCCCCCCEEECCEEEEEEECCCCCCCCHHHHHHHHCCCC
VEFVLAPEPEIMRIIHSQFDTTSSSAKEMLNEMEGSFSELAEAFENETLDLSDDAPIIKM
EEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCHHHHH
VNVILSQAVNERASDIHIEPYEKSLVVRYRVDGILHNVLSPPKSYHAGISSRIKIMSNLN
HHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHHHHHCCCHHHHCCHHHHHHHHHCCC
IAENRLPQDGRIKLRLVGKDIDIRVSTIPCQFGERIVMRLLNKTDQKYSLDTMGFYPDLI
HHHHCCCCCCCEEEEEECCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
KSLRSLIYEPHGIVLVTGPTGSGKSTTLYSALSELNTEERNIITCEDPVEYQIEGISQMQ
HHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCEEECCCCCHHHHCCHHHHH
MQEKIGLTFATGLRAILRQDPDVIMVGEIRDEETARIAIQASLTGHLVFSTLHTNDAASA
HHHHHCCHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEECCEEEEEEEECCCHHHHH
ATRLIDMGIEPYLITSTVLGFMAQRLVRVICTQCKEIYKPTASELESIGISRKALKNGSL
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHCCCCC
HRGKGCSHCMGTGFKGRTGIYELLLVNSHIKHAILQGKDAGQLNEIALEHNFQTLKDYGV
CCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHCCH
KKVIDGVTTIDEVLRVT
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1588814; 10984043 [H]