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The map label for this gene is gspD [H]

Identifier: 116328309

GI number: 116328309

Start: 1927717

End: 1929513

Strand: Reverse

Name: gspD [H]

Synonym: LBL_1645

Alternate gene names: 116328309

Gene position: 1929513-1927717 (Counterclockwise)

Preceding gene: 116328310

Following gene: 116328308

Centisome position: 53.38

GC content: 41.18

Gene sequence:

>1797_bases
ATGTACGGAACAATCAGTCAATTTTCAATCTTTAGAATATTCCCCATTCTCATACTTTTATTTTTAGTGTGGGACAAACC
GGTATTTCCTCAGAGCAAGAAAAAATCTTCCGTTAAAACAAGATCGGCCACCGCTCCGGAAGAACCGGCGGAAAAGAGCT
TTTATGCGAATTGGAGAGACACCGAATTGAACGATTTTCTCAAAGGAATGAGCGCTATCTTAAAGAAAAACATTCTTTTA
GATGAGAGTTTAAAAGGTAAAAAGATTACGATCATTTCTCAAAAGGAAATTCCGATTAAAAATGCATTCATCTTTATGAA
ATCAGTTTTAGAATCCCTCGGTTTTGGCGTGGTGGAAGAACCGGATCTGATTTCGATTGTCAAGATTAAGGATGCGCTTG
CAAGATCTCCCGTGGTCCGAGTCGGTAAGGAATTGATTCCTGAAACGGAAGTCGGCGATTACAGAACGATCACTCAGATT
ATTCCTATTGAAAATACAAAACCGGAAGAACTAGAACCGATTCTCAAACGTCTGACGTCTCCAAATACGGATGTAATCGT
TTATAGAAATACGAACACGATTGTTCTTTCCGGTTCCGGAGCTGACATCAATAAATTGCTGGTTTTGATAAACGAACTCG
ACCTGAAATTGGAAGAGGCAAGTCCGGGGGCAATCGCGTCCGCCGGTGACGTGCACATTTATACTCTGGAGCATAGCGAG
GCCGAAAAGATCGCGGCCACTCTCGTTAAATTGGATAACCCTGTGGTTCAATCCGAAGAATTGAGCCCCGAGAAAAAAGT
TCCGGGACAGATACCGATGAAAGTGGAGAAGATCAAGGCAGTAGGTCATAAAGAATCTAATTCCGTTATTGTAACCGCAA
CGAATGCCGAATGGGCCGAGATTCGAAAGATCATTAAAGTTTTGGATTCCGCTCGAAAACAGGTTCTCTTAGAAGTGCTA
ATTGTGGAACTTACTTCTAGCGACTTGAACGACTTCGGGATCGATTGGAGATATAAGGGAGAGGCTTTTGGTCAATTCAA
CTCGGGTCTTTCTAAAGAGGCAAATATCATCAATTCAAACGGACAAATCAATCCAAATGTCAACACACTCAGCGGTTTCT
CTTTGGGGTTCTTAAAAGCAGGTTCGGAACAGATCATCGGAATTTTAAGTGCTAACCAAGGAAATGAAAATTTCAACGTA
TTATCCGCTCCTCAGGTACTCACCGTGGATAATCAAGAAGCGGAAATCAGTGTCGGCCAAGATGTTCCGGTTCGGACGCA
GAGTAGGAATGCCGGTACCGGTGGAACAAATGCGGTTACAGTAGACAACTACGAATATCGTCCCACAGGTATCAAACTCA
AGTTTACTCCGCACGTAAACAAAAACAATAGGATTACTCTCGAACTCTTCCAGGAGATTAAGAATATCGCTGAGATTGCC
CTTGCCGGAGGGAATCCGACTTTCAATCGACGCGAAATCAAAACTTCCATTTCCATTGAAAACACTCAGTCTATCGTGAT
CGGGGGACTTATCTCAAATGATAAACAAAAGAGGATTATTAAAATTCCTTTTCTTGGAGATATTCCGTATTTAGGTCATC
TTTTCAAAAGGACTACCGAAAAAATCAAAAAGACGAACTTGATGGTTTTTATCACACCGCATATACTGGACAGTAGAGAA
AATGCGGATAAGATGACCGTGAAGAAAAAGATGCTACAGGAACAATACGAGCTCGAAAGAGAAAGAATCCTCAATAAAGA
AAAAGAAATCAAATCTTTCTTTGAAAAAGAGGATTAA

Upstream 100 bases:

>100_bases
AAATCTGGGGAGCCGTAAAAACTGCCGATAAGATTACAGTAGATGTAGAAAGAGGTAGTCAGATTCTCAGCTACGAATTT
ATTATCAGAAATTAAAAAAA

Downstream 100 bases:

>100_bases
AATTTGAAAACTCTCGGAGATATCCTAATCGAAGAGGGGATCATATCCGAAAAAGATCTGGAAGATTCCCTTAAGGTTCA
GAAGAAAAATAACCTTCCGC

Product: Type II secretory pathway component, protein D

Products: NA

Alternate protein names: Pullulanase secretion envelope pulD [H]

Number of amino acids: Translated: 598; Mature: 598

Protein sequence:

>598_residues
MYGTISQFSIFRIFPILILLFLVWDKPVFPQSKKKSSVKTRSATAPEEPAEKSFYANWRDTELNDFLKGMSAILKKNILL
DESLKGKKITIISQKEIPIKNAFIFMKSVLESLGFGVVEEPDLISIVKIKDALARSPVVRVGKELIPETEVGDYRTITQI
IPIENTKPEELEPILKRLTSPNTDVIVYRNTNTIVLSGSGADINKLLVLINELDLKLEEASPGAIASAGDVHIYTLEHSE
AEKIAATLVKLDNPVVQSEELSPEKKVPGQIPMKVEKIKAVGHKESNSVIVTATNAEWAEIRKIIKVLDSARKQVLLEVL
IVELTSSDLNDFGIDWRYKGEAFGQFNSGLSKEANIINSNGQINPNVNTLSGFSLGFLKAGSEQIIGILSANQGNENFNV
LSAPQVLTVDNQEAEISVGQDVPVRTQSRNAGTGGTNAVTVDNYEYRPTGIKLKFTPHVNKNNRITLELFQEIKNIAEIA
LAGGNPTFNRREIKTSISIENTQSIVIGGLISNDKQKRIIKIPFLGDIPYLGHLFKRTTEKIKKTNLMVFITPHILDSRE
NADKMTVKKKMLQEQYELERERILNKEKEIKSFFEKED

Sequences:

>Translated_598_residues
MYGTISQFSIFRIFPILILLFLVWDKPVFPQSKKKSSVKTRSATAPEEPAEKSFYANWRDTELNDFLKGMSAILKKNILL
DESLKGKKITIISQKEIPIKNAFIFMKSVLESLGFGVVEEPDLISIVKIKDALARSPVVRVGKELIPETEVGDYRTITQI
IPIENTKPEELEPILKRLTSPNTDVIVYRNTNTIVLSGSGADINKLLVLINELDLKLEEASPGAIASAGDVHIYTLEHSE
AEKIAATLVKLDNPVVQSEELSPEKKVPGQIPMKVEKIKAVGHKESNSVIVTATNAEWAEIRKIIKVLDSARKQVLLEVL
IVELTSSDLNDFGIDWRYKGEAFGQFNSGLSKEANIINSNGQINPNVNTLSGFSLGFLKAGSEQIIGILSANQGNENFNV
LSAPQVLTVDNQEAEISVGQDVPVRTQSRNAGTGGTNAVTVDNYEYRPTGIKLKFTPHVNKNNRITLELFQEIKNIAEIA
LAGGNPTFNRREIKTSISIENTQSIVIGGLISNDKQKRIIKIPFLGDIPYLGHLFKRTTEKIKKTNLMVFITPHILDSRE
NADKMTVKKKMLQEQYELERERILNKEKEIKSFFEKED
>Mature_598_residues
MYGTISQFSIFRIFPILILLFLVWDKPVFPQSKKKSSVKTRSATAPEEPAEKSFYANWRDTELNDFLKGMSAILKKNILL
DESLKGKKITIISQKEIPIKNAFIFMKSVLESLGFGVVEEPDLISIVKIKDALARSPVVRVGKELIPETEVGDYRTITQI
IPIENTKPEELEPILKRLTSPNTDVIVYRNTNTIVLSGSGADINKLLVLINELDLKLEEASPGAIASAGDVHIYTLEHSE
AEKIAATLVKLDNPVVQSEELSPEKKVPGQIPMKVEKIKAVGHKESNSVIVTATNAEWAEIRKIIKVLDSARKQVLLEVL
IVELTSSDLNDFGIDWRYKGEAFGQFNSGLSKEANIINSNGQINPNVNTLSGFSLGFLKAGSEQIIGILSANQGNENFNV
LSAPQVLTVDNQEAEISVGQDVPVRTQSRNAGTGGTNAVTVDNYEYRPTGIKLKFTPHVNKNNRITLELFQEIKNIAEIA
LAGGNPTFNRREIKTSISIENTQSIVIGGLISNDKQKRIIKIPFLGDIPYLGHLFKRTTEKIKKTNLMVFITPHILDSRE
NADKMTVKKKMLQEQYELERERILNKEKEIKSFFEKED

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of pullulanase [H]

COG id: COG1450

COG function: function code NU; Type II secretory pathway, component PulD

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP D family [H]

Homologues:

Organism=Escherichia coli, GI87082242, Length=458, Percent_Identity=28.3842794759825, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI1789793, Length=282, Percent_Identity=22.3404255319149, Blast_Score=85, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001775
- InterPro:   IPR005644
- InterPro:   IPR004846
- InterPro:   IPR013356
- InterPro:   IPR004845 [H]

Pfam domain/function: PF00263 Secretin; PF03958 Secretin_N [H]

EC number: NA

Molecular weight: Translated: 66736; Mature: 66736

Theoretical pI: Translated: 8.55; Mature: 8.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYGTISQFSIFRIFPILILLFLVWDKPVFPQSKKKSSVKTRSATAPEEPAEKSFYANWRD
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TELNDFLKGMSAILKKNILLDESLKGKKITIISQKEIPIKNAFIFMKSVLESLGFGVVEE
CCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCC
PDLISIVKIKDALARSPVVRVGKELIPETEVGDYRTITQIIPIENTKPEELEPILKRLTS
CCCEEEEEHHHHHHCCHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHCC
PNTDVIVYRNTNTIVLSGSGADINKLLVLINELDLKLEEASPGAIASAGDVHIYTLEHSE
CCCCEEEEECCCEEEEECCCCCHHHHHHHHHHCCEEEECCCCCCEECCCCEEEEEECCCH
AEKIAATLVKLDNPVVQSEELSPEKKVPGQIPMKVEKIKAVGHKESNSVIVTATNAEWAE
HHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHCCCCCCCEEEEECCCCHHH
IRKIIKVLDSARKQVLLEVLIVELTSSDLNDFGIDWRYKGEAFGQFNSGLSKEANIINSN
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCEEECCCCCCCCCCCCCCCCEEECCC
GQINPNVNTLSGFSLGFLKAGSEQIIGILSANQGNENFNVLSAPQVLTVDNQEAEISVGQ
CEECCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCCCEEEECCEEEEECCCCCEEECCC
DVPVRTQSRNAGTGGTNAVTVDNYEYRPTGIKLKFTPHVNKNNRITLELFQEIKNIAEIA
CCCCEECCCCCCCCCCCEEEECCEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHEE
LAGGNPTFNRREIKTSISIENTQSIVIGGLISNDKQKRIIKIPFLGDIPYLGHLFKRTTE
EECCCCCCCCCEEEEEEEECCCCEEEEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHH
KIKKTNLMVFITPHILDSRENADKMTVKKKMLQEQYELERERILNKEKEIKSFFEKED
HHHHCCEEEEEECCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCC
>Mature Secondary Structure
MYGTISQFSIFRIFPILILLFLVWDKPVFPQSKKKSSVKTRSATAPEEPAEKSFYANWRD
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TELNDFLKGMSAILKKNILLDESLKGKKITIISQKEIPIKNAFIFMKSVLESLGFGVVEE
CCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCC
PDLISIVKIKDALARSPVVRVGKELIPETEVGDYRTITQIIPIENTKPEELEPILKRLTS
CCCEEEEEHHHHHHCCHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHCC
PNTDVIVYRNTNTIVLSGSGADINKLLVLINELDLKLEEASPGAIASAGDVHIYTLEHSE
CCCCEEEEECCCEEEEECCCCCHHHHHHHHHHCCEEEECCCCCCEECCCCEEEEEECCCH
AEKIAATLVKLDNPVVQSEELSPEKKVPGQIPMKVEKIKAVGHKESNSVIVTATNAEWAE
HHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHCCCCCCCEEEEECCCCHHH
IRKIIKVLDSARKQVLLEVLIVELTSSDLNDFGIDWRYKGEAFGQFNSGLSKEANIINSN
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCEEECCCCCCCCCCCCCCCCEEECCC
GQINPNVNTLSGFSLGFLKAGSEQIIGILSANQGNENFNVLSAPQVLTVDNQEAEISVGQ
CEECCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCCCEEEECCEEEEECCCCCEEECCC
DVPVRTQSRNAGTGGTNAVTVDNYEYRPTGIKLKFTPHVNKNNRITLELFQEIKNIAEIA
CCCCEECCCCCCCCCCCEEEECCEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHEE
LAGGNPTFNRREIKTSISIENTQSIVIGGLISNDKQKRIIKIPFLGDIPYLGHLFKRTTE
EECCCCCCCCCEEEEEEEECCCCEEEEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHH
KIKKTNLMVFITPHILDSRENADKMTVKKKMLQEQYELERERILNKEKEIKSFFEKED
HHHHCCEEEEEECCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2677007 [H]