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The map label for this gene is leuA-2 [H]

Identifier: 116328259

GI number: 116328259

Start: 1860075

End: 1861586

Strand: Reverse

Name: leuA-2 [H]

Synonym: LBL_1587

Alternate gene names: 116328259

Gene position: 1861586-1860075 (Counterclockwise)

Preceding gene: 116328261

Following gene: 116328257

Centisome position: 51.5

GC content: 46.36

Gene sequence:

>1512_bases
ATGGCAACAGATCAAAAGGACTATGTTCGCATATTCGATACCACTCTCAGAGACGGAGAACAATGTCCCGGAGCCGCAAT
GACGGAAAACGAAAAATTGGAAATCGCCGCTCAACTCGCAGCGATGAAAGTGGACATCATAGAAGCCGGATTTCCAATTT
CTTCTCCCGTTCAATTTCAAGCCGTGGAAAGAATTGCAAGAGAAACGGAAGGACCGACCATCGCTGCTCTTGCAAGAGCC
ATGAAAGCCGATATTGAAGCCGCATTTAAAGCCCTACAACCTGCAAAAAAAAGAAGAATTCATACGTTCATCGCTTCCTC
TCCGATCCACATGAAATACAAACTTGGAAAAGAACCCAAAGAAGTTTTAAAAATGGCCGTGGAGGCAGTGACTCTCTGTA
AACAGTTCGTAGACGACGTCGAATTTTCCCCCGAAGACGCAACCAGAAGTGAACCGGAATTTTTAAGAGAACTCTGCGAA
GCAGTAATCGCCGCCGGTGCAACTACAATCAACATTCCGGATACCGTCGGTTATACTACCCCATCCGAATATGGCGGTCT
TTTTAAATTCTTACTCACGAACGTGAGAGGAGCTGAAAAAGCGATTTTTTCCGCACATTGCCACAACGATTTAGGACTTG
CCACCGCAAACTCTTTGGCAGCAGTTCAAAACGGCGCTCGCCAGATAGAATGCACAATCAATGGCATCGGAGAAAGAGCC
GGCAATACCGCCATGGAAGAAGTCGTCATGGCAATGCGAACTAGAAAAGACATGTTCGGAATTCAGACTCGGACCAAAAC
GGAAGAAATCGCAAGGGCTTCCTATCTCGTAAAGACGATTACGGGAATGCTAGTTCAACCGAACAAAGCAATCGTAGGAG
CAAACGCTTTCGCACACGAGTCCGGAATCCATCAAGATGGAGTCATCAAACACAGAGAAACTTACGAGATTATGAAACCC
GAAACCGTTGGACTTTCTTCCAACAGAATGGTTCTCGGAAGACATAGCGGTCGCGCAGGATTCAAGGATCGGATCGTAAA
ACTCGGCTTTTCTCCTCAAGCGGAAGAACTAGAAGCCGCTTACCAAAGGTTTTTGGAAATTGCGGACAGAAAAAAGGAAA
TCTATGACGAAGACATTCGCGCTCTTTTTTCCGAAGAAACCAGAAAGTCAACGGGAGATCGTTTTCAATTGGAAGGTTTT
ACCGTTTCCACAGGAACCAAAAGTACTCCAACCGCGGGTGTGAGAATTTTGATTGACGGACATGTCAGAGAAGAATCTTC
TACTGGGGACGGCCCCGTAGACGCTATCTATAAAGCGATTCAGAAAACGACGGGAATGGACCCGGAAGTTTCCAGACTCG
TTATTTCCCCAGTTACCGAAGGACAAGACGCAATGGCGGAAGCTTCCGTGACCTTGCAATACAAAGGAGATCGTGTCGTT
GGCAAAGGAAGTTCCACAGACATTATCGAGGCTTGCTCCAGGGCTTATATTTCTGCACTGAATCGATTTTAA

Upstream 100 bases:

>100_bases
ATTTCCATTTTAAAAGAATACAAATTCCATCCGTTTAATTAGTTGTCTAAAGAGTAAAAACCCAAAAAACTGGAAGAAGA
ATTTTTTTGCGAGAATCATA

Downstream 100 bases:

>100_bases
TTTTTTAGAATATTCAAAAACTTACTTTTGGGATCCAAAACAAAACAATCTAATCGCGACCATGCGGCAAAGACTTATCC
GCGCGAATTATTGTATTCTA

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 503; Mature: 502

Protein sequence:

>503_residues
MATDQKDYVRIFDTTLRDGEQCPGAAMTENEKLEIAAQLAAMKVDIIEAGFPISSPVQFQAVERIARETEGPTIAALARA
MKADIEAAFKALQPAKKRRIHTFIASSPIHMKYKLGKEPKEVLKMAVEAVTLCKQFVDDVEFSPEDATRSEPEFLRELCE
AVIAAGATTINIPDTVGYTTPSEYGGLFKFLLTNVRGAEKAIFSAHCHNDLGLATANSLAAVQNGARQIECTINGIGERA
GNTAMEEVVMAMRTRKDMFGIQTRTKTEEIARASYLVKTITGMLVQPNKAIVGANAFAHESGIHQDGVIKHRETYEIMKP
ETVGLSSNRMVLGRHSGRAGFKDRIVKLGFSPQAEELEAAYQRFLEIADRKKEIYDEDIRALFSEETRKSTGDRFQLEGF
TVSTGTKSTPTAGVRILIDGHVREESSTGDGPVDAIYKAIQKTTGMDPEVSRLVISPVTEGQDAMAEASVTLQYKGDRVV
GKGSSTDIIEACSRAYISALNRF

Sequences:

>Translated_503_residues
MATDQKDYVRIFDTTLRDGEQCPGAAMTENEKLEIAAQLAAMKVDIIEAGFPISSPVQFQAVERIARETEGPTIAALARA
MKADIEAAFKALQPAKKRRIHTFIASSPIHMKYKLGKEPKEVLKMAVEAVTLCKQFVDDVEFSPEDATRSEPEFLRELCE
AVIAAGATTINIPDTVGYTTPSEYGGLFKFLLTNVRGAEKAIFSAHCHNDLGLATANSLAAVQNGARQIECTINGIGERA
GNTAMEEVVMAMRTRKDMFGIQTRTKTEEIARASYLVKTITGMLVQPNKAIVGANAFAHESGIHQDGVIKHRETYEIMKP
ETVGLSSNRMVLGRHSGRAGFKDRIVKLGFSPQAEELEAAYQRFLEIADRKKEIYDEDIRALFSEETRKSTGDRFQLEGF
TVSTGTKSTPTAGVRILIDGHVREESSTGDGPVDAIYKAIQKTTGMDPEVSRLVISPVTEGQDAMAEASVTLQYKGDRVV
GKGSSTDIIEACSRAYISALNRF
>Mature_502_residues
ATDQKDYVRIFDTTLRDGEQCPGAAMTENEKLEIAAQLAAMKVDIIEAGFPISSPVQFQAVERIARETEGPTIAALARAM
KADIEAAFKALQPAKKRRIHTFIASSPIHMKYKLGKEPKEVLKMAVEAVTLCKQFVDDVEFSPEDATRSEPEFLRELCEA
VIAAGATTINIPDTVGYTTPSEYGGLFKFLLTNVRGAEKAIFSAHCHNDLGLATANSLAAVQNGARQIECTINGIGERAG
NTAMEEVVMAMRTRKDMFGIQTRTKTEEIARASYLVKTITGMLVQPNKAIVGANAFAHESGIHQDGVIKHRETYEIMKPE
TVGLSSNRMVLGRHSGRAGFKDRIVKLGFSPQAEELEAAYQRFLEIADRKKEIYDEDIRALFSEETRKSTGDRFQLEGFT
VSTGTKSTPTAGVRILIDGHVREESSTGDGPVDAIYKAIQKTTGMDPEVSRLVISPVTEGQDAMAEASVTLQYKGDRVVG
KGSSTDIIEACSRAYISALNRF

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=495, Percent_Identity=45.4545454545455, Blast_Score=450, Evalue=1e-127,
Organism=Saccharomyces cerevisiae, GI6320071, Length=370, Percent_Identity=27.5675675675676, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6320019, Length=370, Percent_Identity=27.5675675675676, Blast_Score=132, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6324225, Length=540, Percent_Identity=25.3703703703704, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6324682, Length=539, Percent_Identity=24.860853432282, Blast_Score=127, Evalue=3e-30,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 54987; Mature: 54856

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATDQKDYVRIFDTTLRDGEQCPGAAMTENEKLEIAAQLAAMKVDIIEAGFPISSPVQFQ
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEECCCCCCCCHHHH
AVERIARETEGPTIAALARAMKADIEAAFKALQPAKKRRIHTFIASSPIHMKYKLGKEPK
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCEEEEECCCCHH
EVLKMAVEAVTLCKQFVDDVEFSPEDATRSEPEFLRELCEAVIAAGATTINIPDTVGYTT
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCC
PSEYGGLFKFLLTNVRGAEKAIFSAHCHNDLGLATANSLAAVQNGARQIECTINGIGERA
CHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCC
GNTAMEEVVMAMRTRKDMFGIQTRTKTEEIARASYLVKTITGMLVQPNKAIVGANAFAHE
CCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHEECCCCEEEECCHHHHC
SGIHQDGVIKHRETYEIMKPETVGLSSNRMVLGRHSGRAGFKDRIVKLGFSPQAEELEAA
CCCCCCCCCCCCCHHHHCCCCEECCCCCCEEEECCCCCCCCHHHHEEECCCCCHHHHHHH
YQRFLEIADRKKEIYDEDIRALFSEETRKSTGDRFQLEGFTVSTGTKSTPTAGVRILIDG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCEEEEEEC
HVREESSTGDGPVDAIYKAIQKTTGMDPEVSRLVISPVTEGQDAMAEASVTLQYKGDRVV
CCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHEEECCCCCCCHHHHHCEEEEEECCCEEE
GKGSSTDIIEACSRAYISALNRF
ECCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ATDQKDYVRIFDTTLRDGEQCPGAAMTENEKLEIAAQLAAMKVDIIEAGFPISSPVQFQ
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEECCCCCCCCHHHH
AVERIARETEGPTIAALARAMKADIEAAFKALQPAKKRRIHTFIASSPIHMKYKLGKEPK
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCEEEEECCCCHH
EVLKMAVEAVTLCKQFVDDVEFSPEDATRSEPEFLRELCEAVIAAGATTINIPDTVGYTT
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCC
PSEYGGLFKFLLTNVRGAEKAIFSAHCHNDLGLATANSLAAVQNGARQIECTINGIGERA
CHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCC
GNTAMEEVVMAMRTRKDMFGIQTRTKTEEIARASYLVKTITGMLVQPNKAIVGANAFAHE
CCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHEECCCCEEEECCHHHHC
SGIHQDGVIKHRETYEIMKPETVGLSSNRMVLGRHSGRAGFKDRIVKLGFSPQAEELEAA
CCCCCCCCCCCCCHHHHCCCCEECCCCCCEEEECCCCCCCCHHHHEEECCCCCHHHHHHH
YQRFLEIADRKKEIYDEDIRALFSEETRKSTGDRFQLEGFTVSTGTKSTPTAGVRILIDG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCEEEEEEC
HVREESSTGDGPVDAIYKAIQKTTGMDPEVSRLVISPVTEGQDAMAEASVTLQYKGDRVV
CCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHEEECCCCCCCHHHHHCEEEEEECCCEEE
GKGSSTDIIEACSRAYISALNRF
ECCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA