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The map label for this gene is rsuA [C]

Identifier: 116328224

GI number: 116328224

Start: 1811307

End: 1812041

Strand: Direct

Name: rsuA [C]

Synonym: LBL_1548

Alternate gene names: 116328224

Gene position: 1811307-1812041 (Clockwise)

Preceding gene: 116328221

Following gene: 116328225

Centisome position: 50.11

GC content: 40.27

Gene sequence:

>735_bases
ATGCAGTCCAAAAACGATTCAAACGCCCGAGATGAAATTCGACTGAATCGATTTCTCGCGGATTGTGGTTTAGGTTCTCG
TAGAAAAACAGAAGAACTGATTCTCAGGGGCCAAATTACAATCAACGGTAAACGAGTGACTGATTTGGGAACCAAAATCA
AACCTGGGAGTGACGTGGTTTCTTATTTAGGAAATGTCGTAGAACCGACAAGAAAACCCAAAAGAATCCTAATTTTAAAT
AAACCGGTCGGATATCTTTGTTCTCATGGAGATCGTTTTCACGAGAAGACAGTTTTTTCTCTTCTTCCTTTGACTTATAA
AAATTTTAAAATTGCCGGACGACTCGATCTCAATTCAAGAGGACTCCTCATTTTGACAAACGACGGTGAATTAGCTCAAA
AAATTTCACACCCTTCCAACGGTTCCGAAAAAGAATATCTCGTGACCTTAAACCACGATCCGGGAGAAAAGTGGATTCAG
GCCGCTTTTCAAAAAGGGATTTTGGATGCAGGAGAAATTCTGCGTGCAAAAATGGTTAAACTCGTTCCGGGTAAAAATTG
TGTATATCGTGTTATTCTTGCAGAAGGCAAAAAAAGACAAATCAGAAGAATGTTCCACGCATCTGGAGCGAGCGTGATCG
ATCTGCAAAGAATACGCATCGGTTCGATACAACTGGAAAAACTGGGCATAGAAGAAGGTAAATTTCTTCTAACTGAGACG
GGAATTTGGAAATGA

Upstream 100 bases:

>100_bases
AGCACAGAGTTTCTACTTCTATTTTCGTATGAGAGAGAGTTCGGGTCAATGAAAGTTTTGGATAGAAGATTTTTTGAGTC
TTTTCGAAATGGAAGAATCT

Downstream 100 bases:

>100_bases
ACTTTATCAGCATTGAGTTTCTTTTATTTTTTTTGGCATTCTATCTGATCTACTGGAACATTCCGGGAAAGAATAGAAAG
TATCTCTTGGTCTTAGGATC

Product: pseudouridylate synthase

Products: NA

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MQSKNDSNARDEIRLNRFLADCGLGSRRKTEELILRGQITINGKRVTDLGTKIKPGSDVVSYLGNVVEPTRKPKRILILN
KPVGYLCSHGDRFHEKTVFSLLPLTYKNFKIAGRLDLNSRGLLILTNDGELAQKISHPSNGSEKEYLVTLNHDPGEKWIQ
AAFQKGILDAGEILRAKMVKLVPGKNCVYRVILAEGKKRQIRRMFHASGASVIDLQRIRIGSIQLEKLGIEEGKFLLTET
GIWK

Sequences:

>Translated_244_residues
MQSKNDSNARDEIRLNRFLADCGLGSRRKTEELILRGQITINGKRVTDLGTKIKPGSDVVSYLGNVVEPTRKPKRILILN
KPVGYLCSHGDRFHEKTVFSLLPLTYKNFKIAGRLDLNSRGLLILTNDGELAQKISHPSNGSEKEYLVTLNHDPGEKWIQ
AAFQKGILDAGEILRAKMVKLVPGKNCVYRVILAEGKKRQIRRMFHASGASVIDLQRIRIGSIQLEKLGIEEGKFLLTET
GIWK
>Mature_244_residues
MQSKNDSNARDEIRLNRFLADCGLGSRRKTEELILRGQITINGKRVTDLGTKIKPGSDVVSYLGNVVEPTRKPKRILILN
KPVGYLCSHGDRFHEKTVFSLLPLTYKNFKIAGRLDLNSRGLLILTNDGELAQKISHPSNGSEKEYLVTLNHDPGEKWIQ
AAFQKGILDAGEILRAKMVKLVPGKNCVYRVILAEGKKRQIRRMFHASGASVIDLQRIRIGSIQLEKLGIEEGKFLLTET
GIWK

Specific function: Unknown

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=227, Percent_Identity=37.4449339207048, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1788510, Length=216, Percent_Identity=35.6481481481481, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1787524, Length=221, Percent_Identity=35.7466063348416, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI87081838, Length=179, Percent_Identity=29.0502793296089, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 27400; Mature: 27400

Theoretical pI: Translated: 10.54; Mature: 10.54

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSKNDSNARDEIRLNRFLADCGLGSRRKTEELILRGQITINGKRVTDLGTKIKPGSDVV
CCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHEEEEEEEEECCEEEECCCCCCCCCHHHH
SYLGNVVEPTRKPKRILILNKPVGYLCSHGDRFHEKTVFSLLPLTYKNFKIAGRLDLNSR
HHHHHHCCCCCCCCEEEEEECCHHHHHCCCCHHHHHHHHHHHCCEECCEEEEEEEECCCC
GLLILTNDGELAQKISHPSNGSEKEYLVTLNHDPGEKWIQAAFQKGILDAGEILRAKMVK
CEEEEECCCHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHH
LVPGKNCVYRVILAEGKKRQIRRMFHASGASVIDLQRIRIGSIQLEKLGIEEGKFLLTET
HCCCCCCEEEEEECCCCHHHHHHHHHCCCCCEEEEEEEEECCEEEEECCCCCCCEEEECC
GIWK
CCCC
>Mature Secondary Structure
MQSKNDSNARDEIRLNRFLADCGLGSRRKTEELILRGQITINGKRVTDLGTKIKPGSDVV
CCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHEEEEEEEEECCEEEECCCCCCCCCHHHH
SYLGNVVEPTRKPKRILILNKPVGYLCSHGDRFHEKTVFSLLPLTYKNFKIAGRLDLNSR
HHHHHHCCCCCCCCEEEEEECCHHHHHCCCCHHHHHHHHHHHCCEECCEEEEEEEECCCC
GLLILTNDGELAQKISHPSNGSEKEYLVTLNHDPGEKWIQAAFQKGILDAGEILRAKMVK
CEEEEECCCHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHH
LVPGKNCVYRVILAEGKKRQIRRMFHASGASVIDLQRIRIGSIQLEKLGIEEGKFLLTET
HCCCCCCEEEEEECCCCHHHHHHHHHCCCCCEEEEEEEEECCEEEEECCCCCCCEEEECC
GIWK
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]