Click here to switch to the map view.

The map label for this gene is algI [H]

Identifier: 116328225

GI number: 116328225

Start: 1812038

End: 1813456

Strand: Direct

Name: algI [H]

Synonym: LBL_1549

Alternate gene names: 116328225

Gene position: 1812038-1813456 (Clockwise)

Preceding gene: 116328224

Following gene: 116328226

Centisome position: 50.13

GC content: 39.04

Gene sequence:

>1419_bases
ATGAACTTTATCAGCATTGAGTTTCTTTTATTTTTTTTGGCATTCTATCTGATCTACTGGAACATTCCGGGAAAGAATAG
AAAGTATCTCTTGGTCTTAGGATCCGCGTTTTTTTATTCCTTATTCGGCCTTAATTTTTTATTTCATCTCATTCTGGTCG
TTTTTGCCAACTGGTTCTTATACCGATATCTGTACGAAAAGACTTGGTATGTAAAGGCTATCGTCATTCTCAATATCCTT
AATTTGGGTCTATTCAAATATTTTTATTTATTCATGGAGTTCATCGGTTTTGTATTTTTGATTCCGGTCCTTCAAAAAAA
GACATCGCTTGACGCGGAATTTTCGGCTTTGTTTCATTTAGAGGGATTTGAAGTCGTCCTTCCCGCTACGATCAGTTACT
ATACGTTCCAATTGATCTCTTTTGCGGTGGATTCAAAACGAGAAGGTTTTGATAAGGACGTCAGTCTCACGGGATTTTTC
TCTTTTATCTTTTTCTTTCCTGTGATGATTGCAGGGCCGATTTTACGATTCGATCAAGTTCGGAATCAATTTGAAAATCC
AACAATGACCCCTTCCAAATTGATCGACGGCCTCTGGCTTTTTGTCCGGGGTCTCGTTAAAAAAGGATTATTATCTGCGG
CGGTTCTTCCCTTGATCGCGCCTGTATTCCTATCTCCGAAAGATTATTCCGGAATCGCACTTTTACTGACTTGTTTCCTC
TTTGCAGCCAACCTTTATTTCGACTTTTCGGGACTTACGGATATGGCGAGAGGGATCGGAAAGTTAATGGGGTTCGATCT
ACCGGAGAATTTTAAGGCTCCGTTTTTCTTCCGGAGCTTCGGAGATCTTTGGCGTAGATGGCATCTAACGTTTTCCTTTT
GGATTCGAGACTATATTTATATTCCATTAGGTGGATCGAAAAAGGGAGAAATTAGAACGGCCATTAATCTTATCGTAACC
TTCATGTTAGGCGGACTTTGGCACGGGGCAAGTTTGAACTTTCTCATATGGGGCCTGCTCACAGGTATTTATTTATCTTT
GGAAAGATTATTCGAAGTGCGAAATTGGAGAATTCTTCCTGAAATTCCTTATGTAAAAGCGGTTATACGTTATGCTTTCG
TGCTTGTCGTATATTCGATTTCTTGGACTTTCTTTTTTACACCAAATTTTAATTCCGCGACGTCTTCCATTGCAAGAATT
TTGACGTTTCAAAACGGACAACCTTTGATAGGATTCGAAACGGGAATGTATATGCTTCTTTTCGTATTTTTATTTCATAT
ACCTGAAGAATGGCCTGAAAAATATTCGGTTCCCGAAATCTGGAGAGCGAGATTTTTGCCGATTTTAGGCCTGATTGTTC
TTTTTATCATGATTGGAACGAATGCGGGAAACGCGGATTTTTTCTATTCAAGGTTTTAG

Upstream 100 bases:

>100_bases
GCGTGATCGATCTGCAAAGAATACGCATCGGTTCGATACAACTGGAAAAACTGGGCATAGAAGAAGGTAAATTTCTTCTA
ACTGAGACGGGAATTTGGAA

Downstream 100 bases:

>100_bases
GTTTTAACTGGTTTTATCTATGAAGAAAATTTATATCTATTATCCGATTCTATTTCTCGTATTCATATTTTGTTTGGATA
AAATATTTACTCTCGAATAT

Product: acyltransferase

Products: NA

Alternate protein names: Alginate biosynthesis protein AlgI [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MNFISIEFLLFFLAFYLIYWNIPGKNRKYLLVLGSAFFYSLFGLNFLFHLILVVFANWFLYRYLYEKTWYVKAIVILNIL
NLGLFKYFYLFMEFIGFVFLIPVLQKKTSLDAEFSALFHLEGFEVVLPATISYYTFQLISFAVDSKREGFDKDVSLTGFF
SFIFFFPVMIAGPILRFDQVRNQFENPTMTPSKLIDGLWLFVRGLVKKGLLSAAVLPLIAPVFLSPKDYSGIALLLTCFL
FAANLYFDFSGLTDMARGIGKLMGFDLPENFKAPFFFRSFGDLWRRWHLTFSFWIRDYIYIPLGGSKKGEIRTAINLIVT
FMLGGLWHGASLNFLIWGLLTGIYLSLERLFEVRNWRILPEIPYVKAVIRYAFVLVVYSISWTFFFTPNFNSATSSIARI
LTFQNGQPLIGFETGMYMLLFVFLFHIPEEWPEKYSVPEIWRARFLPILGLIVLFIMIGTNAGNADFFYSRF

Sequences:

>Translated_472_residues
MNFISIEFLLFFLAFYLIYWNIPGKNRKYLLVLGSAFFYSLFGLNFLFHLILVVFANWFLYRYLYEKTWYVKAIVILNIL
NLGLFKYFYLFMEFIGFVFLIPVLQKKTSLDAEFSALFHLEGFEVVLPATISYYTFQLISFAVDSKREGFDKDVSLTGFF
SFIFFFPVMIAGPILRFDQVRNQFENPTMTPSKLIDGLWLFVRGLVKKGLLSAAVLPLIAPVFLSPKDYSGIALLLTCFL
FAANLYFDFSGLTDMARGIGKLMGFDLPENFKAPFFFRSFGDLWRRWHLTFSFWIRDYIYIPLGGSKKGEIRTAINLIVT
FMLGGLWHGASLNFLIWGLLTGIYLSLERLFEVRNWRILPEIPYVKAVIRYAFVLVVYSISWTFFFTPNFNSATSSIARI
LTFQNGQPLIGFETGMYMLLFVFLFHIPEEWPEKYSVPEIWRARFLPILGLIVLFIMIGTNAGNADFFYSRF
>Mature_472_residues
MNFISIEFLLFFLAFYLIYWNIPGKNRKYLLVLGSAFFYSLFGLNFLFHLILVVFANWFLYRYLYEKTWYVKAIVILNIL
NLGLFKYFYLFMEFIGFVFLIPVLQKKTSLDAEFSALFHLEGFEVVLPATISYYTFQLISFAVDSKREGFDKDVSLTGFF
SFIFFFPVMIAGPILRFDQVRNQFENPTMTPSKLIDGLWLFVRGLVKKGLLSAAVLPLIAPVFLSPKDYSGIALLLTCFL
FAANLYFDFSGLTDMARGIGKLMGFDLPENFKAPFFFRSFGDLWRRWHLTFSFWIRDYIYIPLGGSKKGEIRTAINLIVT
FMLGGLWHGASLNFLIWGLLTGIYLSLERLFEVRNWRILPEIPYVKAVIRYAFVLVVYSISWTFFFTPNFNSATSSIARI
LTFQNGQPLIGFETGMYMLLFVFLFHIPEEWPEKYSVPEIWRARFLPILGLIVLFIMIGTNAGNADFFYSRF

Specific function: Together with AlgJ and AlgF, forms an inner membrane complex which probably interacts with the alginate polymerization- transport complex and adds acetyl groups at the O-2 and O-3 positions of polymannuronic acid. Acetylation of alginate is important for

COG id: COG1696

COG function: function code M; Predicted membrane protein involved in D-alanine export

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane-bound acyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004299 [H]

Pfam domain/function: PF03062 MBOAT [H]

EC number: NA

Molecular weight: Translated: 55025; Mature: 55025

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCC
>Mature Secondary Structure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HHHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]