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The map label for this gene is prfA [H]

Identifier: 116328132

GI number: 116328132

Start: 1663526

End: 1663990

Strand: Direct

Name: prfA [H]

Synonym: LBL_1430

Alternate gene names: 116328132

Gene position: 1663526-1663990 (Clockwise)

Preceding gene: 116328131

Following gene: 116328133

Centisome position: 46.02

GC content: 43.23

Gene sequence:

>465_bases
ATGGCTTCTCGTTTTCCCGTTTCGGTCGAAAAGGAATCTAAGCTTTTGGAACTCATGGATGTTCTGGAAGTAAAAGAAAA
CGAGCTAGAGGAGAGTTTTACGAGAAGCGGGGGCAAGGGCGGGCAAAATGTAAATAAGGTTTCCACCGCGGTTCATCTCA
AGCATAAACCTACCGGAATTGAAGTCAAATGTTCTCTCTACAGAACCCAAGGTCTCAATCGTTATAAGGCGCGTGCCATT
CTTTGTGAAAAAATCCAAGAAGAGAATCGAAAGAAACAAGGCACGGTCAGCGACGAACGCAAAAACACGATTCGAAACAA
ACAAAAAGACGCAAAACGAAAGAAAGAAAAGTATTCCAGAAAGGATCAGAATTCTTCTTCCATTCTCACCGAGCTCAAAG
AAAGTTTTGAACCGGGGTGGGACGACGATTCGGAAGTTTCGAATGAAAGCAAGGTCGAAAATTGA

Upstream 100 bases:

>100_bases
TCATCGATTGCAAAGTTACTTATGTTTGTCTGGATGAAAATGGAAGACCGTTCCCTTATTTCAAAGACCATTTCGAATTT
CGGGATCTGAAATAATCTTT

Downstream 100 bases:

>100_bases
AACGATCGTTTTGAATTTCGAATTTTGTCTAACTAAGGGAGATGGTCGTTTAAACTCGAAATCACCTGTCGATTTTCGAT
AAAGTAAAAACCGAGTTCTA

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 154; Mature: 153

Protein sequence:

>154_residues
MASRFPVSVEKESKLLELMDVLEVKENELEESFTRSGGKGGQNVNKVSTAVHLKHKPTGIEVKCSLYRTQGLNRYKARAI
LCEKIQEENRKKQGTVSDERKNTIRNKQKDAKRKKEKYSRKDQNSSSILTELKESFEPGWDDDSEVSNESKVEN

Sequences:

>Translated_154_residues
MASRFPVSVEKESKLLELMDVLEVKENELEESFTRSGGKGGQNVNKVSTAVHLKHKPTGIEVKCSLYRTQGLNRYKARAI
LCEKIQEENRKKQGTVSDERKNTIRNKQKDAKRKKEKYSRKDQNSSSILTELKESFEPGWDDDSEVSNESKVEN
>Mature_153_residues
ASRFPVSVEKESKLLELMDVLEVKENELEESFTRSGGKGGQNVNKVSTAVHLKHKPTGIEVKCSLYRTQGLNRYKARAIL
CEKIQEENRKKQGTVSDERKNTIRNKQKDAKRKKEKYSRKDQNSSSILTELKESFEPGWDDDSEVSNESKVEN

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI303304971, Length=65, Percent_Identity=52.3076923076923, Blast_Score=63, Evalue=8e-11,
Organism=Homo sapiens, GI219879802, Length=65, Percent_Identity=52.3076923076923, Blast_Score=63, Evalue=8e-11,
Organism=Homo sapiens, GI22748627, Length=65, Percent_Identity=52.3076923076923, Blast_Score=63, Evalue=8e-11,
Organism=Drosophila melanogaster, GI24654113, Length=113, Percent_Identity=36.283185840708, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 17663; Mature: 17532

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASRFPVSVEKESKLLELMDVLEVKENELEESFTRSGGKGGQNVNKVSTAVHLKHKPTGI
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCE
EVKCSLYRTQGLNRYKARAILCEKIQEENRKKQGTVSDERKNTIRNKQKDAKRKKEKYSR
EEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KDQNSSSILTELKESFEPGWDDDSEVSNESKVEN
CCCCHHHHHHHHHHHCCCCCCCCCCCCCHHCCCC
>Mature Secondary Structure 
ASRFPVSVEKESKLLELMDVLEVKENELEESFTRSGGKGGQNVNKVSTAVHLKHKPTGI
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCE
EVKCSLYRTQGLNRYKARAILCEKIQEENRKKQGTVSDERKNTIRNKQKDAKRKKEKYSR
EEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KDQNSSSILTELKESFEPGWDDDSEVSNESKVEN
CCCCHHHHHHHHHHHCCCCCCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA