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The map label for this gene is leuB-1 [H]

Identifier: 116328123

GI number: 116328123

Start: 1654727

End: 1655803

Strand: Reverse

Name: leuB-1 [H]

Synonym: LBL_1421

Alternate gene names: 116328123

Gene position: 1655803-1654727 (Counterclockwise)

Preceding gene: 116328124

Following gene: 116328122

Centisome position: 45.81

GC content: 45.96

Gene sequence:

>1077_bases
ATGAAGAATGTAGCGGTACTTTCCGGAGACGGAATCGGACCGGAAGTCATGGAAGTAGCCCTCTCCGTTTTGAAAAAGGC
TCTCAGTGCAAAAGCTTCCGAATTTTATTTTAAAGAAGGTTTAGTGGGCGGAATTGCAATCGATAAAACTGGACATCCCC
TTCCTCCGGAAACTCTCAAACTCTGCGAAGAATCCTCTGCGATTCTTTTCGGAAGTGTAGGAGGACCGAAATGGGAAACT
CTCCCTCCGGAAAAACAACCGGAACGAGGAGCGCTTCTACCTCTCCGAAAACATTTCGACTTATTTGCAAATCTCAGACC
GGCAATCATTTATCCCGAACTCAAAAATGCTTCCCCTGTTCGAGCCGACATTATCGGAAACGGGCTGGATATTCTGATCC
TAAGGGAATTGACCGGAGGAATTTATTTTGGTCAACCGAAAGGAAGGGAAGGCGTCGGCCAGGAAGAGTTCGCTTATGAC
ACGATGAAATATTCTAGAAAAGAAATAGAAAGAATTACCAGAGTCGCATTCCAAGCGGCGAGGAAAAGAAACAACAAAGT
CACGAGCATTGACAAAGCAAACGTCTTGACGACTTCCGTTTTTTGGAAAGAAGTTGTAATCGATTTGCATAAGAAAGAAT
TTTCTGACGTCCAATTGAATCATCTTTATGTGGACAATGCCGCGATGCAGCTCATCGTAAATCCGAAACAATTCGACGTG
GTTCTTTGCGAGAATATGTTCGGGGACATACTTTCGGACGAAGCTTCCATCATTACCGGCTCGATCGGGATGCTTCCATC
GGCTTCTCTTTCGGAATCAGGATTCGGTTTGTATGAACCTTCTGGCGGTTCCGCTCCGGACATCGCAGGAAAAGGTGTAG
CCAATCCGATCGCTCAAGTCCTCAGCGCCGCGCTGATGTTACGGTATTCTTTCTCCATGGAAGAAGAAGCGACCAAAATA
GAAACCGCAGTCCGAAAAACGATCGCCTCCGGAAAAAGAACCAGAGACATCGCGGAAGTGGATTCTACAGTTGTCGGAAC
AAAAGAAATCGGTCAAGTAATCGAATCCTTTCTCTAA

Upstream 100 bases:

>100_bases
GAATCATGCCCTCACTTACGATTTCCACAGACTTTTTAAATCAGGGATTGGACATTTTAGAATCCGTACTCAAACAAAAT
TAAAAGGATCCAAAAAAAGA

Downstream 100 bases:

>100_bases
AAAGAGGTAAATATAAATGAAAGCAGGTGTAGCTCCCAACGGAAGACCATATCAGGTACTGATTGCCGAAAACTCCAGAT
TTCAAGCCAAACAGTTGGCT

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MKNVAVLSGDGIGPEVMEVALSVLKKALSAKASEFYFKEGLVGGIAIDKTGHPLPPETLKLCEESSAILFGSVGGPKWET
LPPEKQPERGALLPLRKHFDLFANLRPAIIYPELKNASPVRADIIGNGLDILILRELTGGIYFGQPKGREGVGQEEFAYD
TMKYSRKEIERITRVAFQAARKRNNKVTSIDKANVLTTSVFWKEVVIDLHKKEFSDVQLNHLYVDNAAMQLIVNPKQFDV
VLCENMFGDILSDEASIITGSIGMLPSASLSESGFGLYEPSGGSAPDIAGKGVANPIAQVLSAALMLRYSFSMEEEATKI
ETAVRKTIASGKRTRDIAEVDSTVVGTKEIGQVIESFL

Sequences:

>Translated_358_residues
MKNVAVLSGDGIGPEVMEVALSVLKKALSAKASEFYFKEGLVGGIAIDKTGHPLPPETLKLCEESSAILFGSVGGPKWET
LPPEKQPERGALLPLRKHFDLFANLRPAIIYPELKNASPVRADIIGNGLDILILRELTGGIYFGQPKGREGVGQEEFAYD
TMKYSRKEIERITRVAFQAARKRNNKVTSIDKANVLTTSVFWKEVVIDLHKKEFSDVQLNHLYVDNAAMQLIVNPKQFDV
VLCENMFGDILSDEASIITGSIGMLPSASLSESGFGLYEPSGGSAPDIAGKGVANPIAQVLSAALMLRYSFSMEEEATKI
ETAVRKTIASGKRTRDIAEVDSTVVGTKEIGQVIESFL
>Mature_358_residues
MKNVAVLSGDGIGPEVMEVALSVLKKALSAKASEFYFKEGLVGGIAIDKTGHPLPPETLKLCEESSAILFGSVGGPKWET
LPPEKQPERGALLPLRKHFDLFANLRPAIIYPELKNASPVRADIIGNGLDILILRELTGGIYFGQPKGREGVGQEEFAYD
TMKYSRKEIERITRVAFQAARKRNNKVTSIDKANVLTTSVFWKEVVIDLHKKEFSDVQLNHLYVDNAAMQLIVNPKQFDV
VLCENMFGDILSDEASIITGSIGMLPSASLSESGFGLYEPSGGSAPDIAGKGVANPIAQVLSAALMLRYSFSMEEEATKI
ETAVRKTIASGKRTRDIAEVDSTVVGTKEIGQVIESFL

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=349, Percent_Identity=32.0916905444126, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI28178821, Length=351, Percent_Identity=29.0598290598291, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI28178816, Length=330, Percent_Identity=29.3939393939394, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI28178819, Length=187, Percent_Identity=32.620320855615, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI4758582, Length=354, Percent_Identity=26.8361581920904, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI28178838, Length=327, Percent_Identity=27.5229357798165, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI87081683, Length=356, Percent_Identity=61.2359550561798, Blast_Score=452, Evalue=1e-128,
Organism=Escherichia coli, GI1788101, Length=344, Percent_Identity=33.4302325581395, Blast_Score=163, Evalue=1e-41,
Organism=Escherichia coli, GI1787381, Length=355, Percent_Identity=25.0704225352113, Blast_Score=85, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI25144293, Length=368, Percent_Identity=29.0760869565217, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI71986051, Length=340, Percent_Identity=29.7058823529412, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17505779, Length=346, Percent_Identity=29.4797687861272, Blast_Score=111, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17550882, Length=363, Percent_Identity=28.9256198347107, Blast_Score=107, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6319830, Length=363, Percent_Identity=42.9752066115703, Blast_Score=268, Evalue=1e-72,
Organism=Saccharomyces cerevisiae, GI6322097, Length=353, Percent_Identity=32.8611898016997, Blast_Score=147, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6324291, Length=280, Percent_Identity=35, Blast_Score=138, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6324709, Length=348, Percent_Identity=30.7471264367816, Blast_Score=122, Evalue=8e-29,
Organism=Drosophila melanogaster, GI24643268, Length=335, Percent_Identity=32.2388059701493, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24643270, Length=335, Percent_Identity=32.2388059701493, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI24661184, Length=343, Percent_Identity=28.2798833819242, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI161078637, Length=272, Percent_Identity=30.1470588235294, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI161078635, Length=272, Percent_Identity=30.1470588235294, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI161078639, Length=272, Percent_Identity=30.1470588235294, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI161078633, Length=272, Percent_Identity=30.1470588235294, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24650122, Length=272, Percent_Identity=30.1470588235294, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI281362242, Length=354, Percent_Identity=25.7062146892655, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24648872, Length=354, Percent_Identity=25.7062146892655, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI20130355, Length=329, Percent_Identity=25.2279635258359, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 38889; Mature: 38889

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNVAVLSGDGIGPEVMEVALSVLKKALSAKASEFYFKEGLVGGIAIDKTGHPLPPETLK
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHH
LCEESSAILFGSVGGPKWETLPPEKQPERGALLPLRKHFDLFANLRPAIIYPELKNASPV
HHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCC
RADIIGNGLDILILRELTGGIYFGQPKGREGVGQEEFAYDTMKYSRKEIERITRVAFQAA
CHHHCCCCCEEEEEEEHHCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKRNNKVTSIDKANVLTTSVFWKEVVIDLHKKEFSDVQLNHLYVDNAAMQLIVNPKQFDV
HHCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCEEEEEECCCHHHE
VLCENMFGDILSDEASIITGSIGMLPSASLSESGFGLYEPSGGSAPDIAGKGVANPIAQV
EEHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHH
LSAALMLRYSFSMEEEATKIETAVRKTIASGKRTRDIAEVDSTVVGTKEIGQVIESFL
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHC
>Mature Secondary Structure
MKNVAVLSGDGIGPEVMEVALSVLKKALSAKASEFYFKEGLVGGIAIDKTGHPLPPETLK
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHH
LCEESSAILFGSVGGPKWETLPPEKQPERGALLPLRKHFDLFANLRPAIIYPELKNASPV
HHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCC
RADIIGNGLDILILRELTGGIYFGQPKGREGVGQEEFAYDTMKYSRKEIERITRVAFQAA
CHHHCCCCCEEEEEEEHHCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKRNNKVTSIDKANVLTTSVFWKEVVIDLHKKEFSDVQLNHLYVDNAAMQLIVNPKQFDV
HHCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCEEEEEECCCHHHE
VLCENMFGDILSDEASIITGSIGMLPSASLSESGFGLYEPSGGSAPDIAGKGVANPIAQV
EEHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHH
LSAALMLRYSFSMEEEATKIETAVRKTIASGKRTRDIAEVDSTVVGTKEIGQVIESFL
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA