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The map label for this gene is mdh

Identifier: 116328112

GI number: 116328112

Start: 1642954

End: 1643934

Strand: Direct

Name: mdh

Synonym: LBL_1410

Alternate gene names: 116328112

Gene position: 1642954-1643934 (Clockwise)

Preceding gene: 116328111

Following gene: 116328113

Centisome position: 45.46

GC content: 45.06

Gene sequence:

>981_bases
ATGGGTAAGACGGTGAAGGTTGCTGTTACAGGCGCTGCGGGACAAATTGGATATTCGCTTCTTTTTAGAATCGCTTCCGG
GCAGATGTTTGGTACCGATACTGCGGTGGAAATTCAGATGCTTGAGTTGGAAGCGGCAATACCCGCGGCAAAAGGTGTGA
TTATGGAATTGGAAGACTGCGCATTTCCTCTTTTGCAAAAAGTAACCGTTTCTTCCGATTTGGATATTGCTTTTAAAGAC
ATCAATTGGGCGTTGTTAGTCGGTTCCGTTCCTAGAAAAGCGGGAATGGAAAGAGGAGATCTTCTCAAAATTAACGGAGG
AATTTTTATAAATCAAGGAAAGGCGATCGAAAAGAACGCGGCTTCCGACGTAAGGGTTCTCGTGGTTGGTAACCCTTGTA
ATACGAATTGTCTGATCGCAATGAACAACGCAAAAGGAATACCGTCAGATCGTTGGTTTGCAATGACTAAACTAGATGAG
AACAGAGCGAAGTCACAGCTTGCTTCTAAAGCGGGTGTTCCCGTAAAAGAGGTAACCCATTTGGGAATTTGGGGAAATCA
TTCCTCTACTCAGTATCCGGATTTTTACAATGCAAAAATTTCCGGCAAACCGGTAACCGACGTAATTTCCGACCATGAAT
GGTTAAAGGGAGATTTCATTAAAAACGTTCAACAAAGAGGAGCCGAGATCATCAAAGCGAGGGGAGCTTCTTCTGCGGCA
AGTGCGGCAAATGGAGTTGTAGATACCGTCCGTGCAATCATTACTCCGACGGCTTCCGGTGATGCCTTTTCAGCGGCCAT
CGCTTCCGACGGGTCTTACGGAACTGAAAAAGGCCTCATCTTCGGATTTCCTTTGAAGTCCGATGGTAAGAAAGTGGGGA
TCGTCCAAGGTCTTCCATTCAACGACTTTGCGAAGGAAAAATTCAAAACTACTCATGATGAACTCATTTCCGAAAGAAAT
GAAGTGAAGGACATGCTCTAG

Upstream 100 bases:

>100_bases
ATGGGATTATGGTGGAAGGTGCACAACCTATCGAAGCGTTTACGAAGGTGATTGATCAGGAACTTAAAAATTAAAATTGG
AAAATAGGAGCTAGTCAAAT

Downstream 100 bases:

>100_bases
AATTCAAAAGAGAGGTTTTTTGAACCTGAATCCGTCTACATTCTTTCAGAAAGCCTCTTTTGTTTTAGAATCTCTGAAAG
AGAATTTTCTTTTTCGATCC

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MGKTVKVAVTGAAGQIGYSLLFRIASGQMFGTDTAVEIQMLELEAAIPAAKGVIMELEDCAFPLLQKVTVSSDLDIAFKD
INWALLVGSVPRKAGMERGDLLKINGGIFINQGKAIEKNAASDVRVLVVGNPCNTNCLIAMNNAKGIPSDRWFAMTKLDE
NRAKSQLASKAGVPVKEVTHLGIWGNHSSTQYPDFYNAKISGKPVTDVISDHEWLKGDFIKNVQQRGAEIIKARGASSAA
SAANGVVDTVRAIITPTASGDAFSAAIASDGSYGTEKGLIFGFPLKSDGKKVGIVQGLPFNDFAKEKFKTTHDELISERN
EVKDML

Sequences:

>Translated_326_residues
MGKTVKVAVTGAAGQIGYSLLFRIASGQMFGTDTAVEIQMLELEAAIPAAKGVIMELEDCAFPLLQKVTVSSDLDIAFKD
INWALLVGSVPRKAGMERGDLLKINGGIFINQGKAIEKNAASDVRVLVVGNPCNTNCLIAMNNAKGIPSDRWFAMTKLDE
NRAKSQLASKAGVPVKEVTHLGIWGNHSSTQYPDFYNAKISGKPVTDVISDHEWLKGDFIKNVQQRGAEIIKARGASSAA
SAANGVVDTVRAIITPTASGDAFSAAIASDGSYGTEKGLIFGFPLKSDGKKVGIVQGLPFNDFAKEKFKTTHDELISERN
EVKDML
>Mature_325_residues
GKTVKVAVTGAAGQIGYSLLFRIASGQMFGTDTAVEIQMLELEAAIPAAKGVIMELEDCAFPLLQKVTVSSDLDIAFKDI
NWALLVGSVPRKAGMERGDLLKINGGIFINQGKAIEKNAASDVRVLVVGNPCNTNCLIAMNNAKGIPSDRWFAMTKLDEN
RAKSQLASKAGVPVKEVTHLGIWGNHSSTQYPDFYNAKISGKPVTDVISDHEWLKGDFIKNVQQRGAEIIKARGASSAAS
AANGVVDTVRAIITPTASGDAFSAAIASDGSYGTEKGLIFGFPLKSDGKKVGIVQGLPFNDFAKEKFKTTHDELISERNE
VKDML

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 2 family

Homologues:

Organism=Homo sapiens, GI5174539, Length=331, Percent_Identity=48.036253776435, Blast_Score=307, Evalue=8e-84,
Organism=Homo sapiens, GI89886456, Length=331, Percent_Identity=25.6797583081571, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI1789632, Length=244, Percent_Identity=27.0491803278689, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17561064, Length=326, Percent_Identity=46.319018404908, Blast_Score=260, Evalue=5e-70,
Organism=Caenorhabditis elegans, GI32566798, Length=239, Percent_Identity=46.4435146443515, Blast_Score=207, Evalue=7e-54,
Organism=Caenorhabditis elegans, GI32566800, Length=171, Percent_Identity=45.6140350877193, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24583394, Length=330, Percent_Identity=47.8787878787879, Blast_Score=297, Evalue=8e-81,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_LEPBJ (Q04RS5)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_801153.1
- ProteinModelPortal:   Q04RS5
- STRING:   Q04RS5
- GeneID:   4410114
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_1874
- eggNOG:   COG0039
- HOGENOM:   HBG289884
- OMA:   MIIWGNH
- ProtClustDB:   PRK05442
- BioCyc:   LBOR355277:LBJ_1874-MONOMER
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_01517
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR001252
- InterPro:   IPR010945
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR23382
- PIRSF:   PIRSF000102
- TIGRFAMs:   TIGR01759

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 34791; Mature: 34660

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: PS00068 MDH

Important sites: ACT_SITE 187-187 BINDING 92-92 BINDING 98-98 BINDING 105-105 BINDING 112-112 BINDING 131-131 BINDING 162-162

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKTVKVAVTGAAGQIGYSLLFRIASGQMFGTDTAVEIQMLELEAAIPAAKGVIMELEDC
CCCEEEEEEECCCHHHHHHHHHHHHCCCEECCCCEEEEEEEEEEHHCCCCCCEEEEHHHH
AFPLLQKVTVSSDLDIAFKDINWALLVGSVPRKAGMERGDLLKINGGIFINQGKAIEKNA
HHHHHHHHHCCCCCCEEEECCCEEEEEECCCHHCCCCCCCEEEECCCEEEECCCCCCCCC
ASDVRVLVVGNPCNTNCLIAMNNAKGIPSDRWFAMTKLDENRAKSQLASKAGVPVKEVTH
CCCEEEEEECCCCCCEEEEEECCCCCCCCCCEEEEEECCHHHHHHHHHHHCCCCHHHHEE
LGIWGNHSSTQYPDFYNAKISGKPVTDVISDHEWLKGDFIKNVQQRGAEIIKARGASSAA
EEEECCCCCCCCCCCCCCEECCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHH
SAANGVVDTVRAIITPTASGDAFSAAIASDGSYGTEKGLIFGFPLKSDGKKVGIVQGLPF
HHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCEEEECCCCCCCCEEEEEECCCC
NDFAKEKFKTTHDELISERNEVKDML
CHHHHHHHCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GKTVKVAVTGAAGQIGYSLLFRIASGQMFGTDTAVEIQMLELEAAIPAAKGVIMELEDC
CCEEEEEEECCCHHHHHHHHHHHHCCCEECCCCEEEEEEEEEEHHCCCCCCEEEEHHHH
AFPLLQKVTVSSDLDIAFKDINWALLVGSVPRKAGMERGDLLKINGGIFINQGKAIEKNA
HHHHHHHHHCCCCCCEEEECCCEEEEEECCCHHCCCCCCCEEEECCCEEEECCCCCCCCC
ASDVRVLVVGNPCNTNCLIAMNNAKGIPSDRWFAMTKLDENRAKSQLASKAGVPVKEVTH
CCCEEEEEECCCCCCEEEEEECCCCCCCCCCEEEEEECCHHHHHHHHHHHCCCCHHHHEE
LGIWGNHSSTQYPDFYNAKISGKPVTDVISDHEWLKGDFIKNVQQRGAEIIKARGASSAA
EEEECCCCCCCCCCCCCCEECCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHH
SAANGVVDTVRAIITPTASGDAFSAAIASDGSYGTEKGLIFGFPLKSDGKKVGIVQGLPF
HHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCEEEECCCCCCCCEEEEEECCCC
NDFAKEKFKTTHDELISERNEVKDML
CHHHHHHHCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA