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The map label for this gene is queF

Identifier: 116328065

GI number: 116328065

Start: 1592395

End: 1592796

Strand: Reverse

Name: queF

Synonym: LBL_1360

Alternate gene names: 116328065

Gene position: 1592796-1592395 (Counterclockwise)

Preceding gene: 116328067

Following gene: 116328064

Centisome position: 44.07

GC content: 41.04

Gene sequence:

>402_bases
ATGAAAACGAATCATCCAGAGACCTACGATGGAAGACAAGATCACATCCCTTCTCTGAAAACTCCCGAAATCGAATCTTT
CACCAATGTTTACGAAGGAAAGGATTATACAATCGATTTTACGGTTCCGGAGTTTACGGCGGTTTGTCCGAAGACCGGGC
TTCCCGATTTCGGAGTCATTTACGTTTCCTACGTCCCCACAAAACGTTGCATCGAGCTTAAGTCCTTTAAAGAATACATT
CTAAGTTATAGAAACGTCGGTGTTTTTCACGAATTTTTAGTCAATAAGATCATGGAAGATCTTATCGCAGCGATCGATCC
GAAATACTTAAAAGTGATCGGAGATTACAACGCCAGAGGCGGAATCAAAACCGTCGTCACTCGAGAATATAATAAGATTT
AA

Upstream 100 bases:

>100_bases
TTCTTCCGGACTTCCATTCCACCCCTCTTTGGGACTTTGATACGAATTCAATTTGGGAAAAAGAATTTTCGAGGAGAAAA
GATCGAAAAATCTGGTCATC

Downstream 100 bases:

>100_bases
TCCGACATGGACTCTATTACATTTTTAGGTTATATCGCATCTTTACTGACGACTGTCTCTTTTTTACCCCAACTGATCCG
TATCACTATGGGTGGAAATA

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase

Number of amino acids: Translated: 133; Mature: 133

Protein sequence:

>133_residues
MKTNHPETYDGRQDHIPSLKTPEIESFTNVYEGKDYTIDFTVPEFTAVCPKTGLPDFGVIYVSYVPTKRCIELKSFKEYI
LSYRNVGVFHEFLVNKIMEDLIAAIDPKYLKVIGDYNARGGIKTVVTREYNKI

Sequences:

>Translated_133_residues
MKTNHPETYDGRQDHIPSLKTPEIESFTNVYEGKDYTIDFTVPEFTAVCPKTGLPDFGVIYVSYVPTKRCIELKSFKEYI
LSYRNVGVFHEFLVNKIMEDLIAAIDPKYLKVIGDYNARGGIKTVVTREYNKI
>Mature_133_residues
MKTNHPETYDGRQDHIPSLKTPEIESFTNVYEGKDYTIDFTVPEFTAVCPKTGLPDFGVIYVSYVPTKRCIELKSFKEYI
LSYRNVGVFHEFLVNKIMEDLIAAIDPKYLKVIGDYNARGGIKTVVTREYNKI

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1)

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEF_LEPBJ (Q04RM8)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_801200.1
- ProteinModelPortal:   Q04RM8
- SMR:   Q04RM8
- STRING:   Q04RM8
- GeneID:   4411876
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_1924
- eggNOG:   COG0780
- HOGENOM:   HBG294103
- OMA:   FRNAGIF
- ProtClustDB:   PRK13258
- BioCyc:   LBOR355277:LBJ_1924-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00818
- InterPro:   IPR016856
- InterPro:   IPR020602
- PIRSF:   PIRSF027377
- TIGRFAMs:   TIGR03139

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =1.7.1.13

Molecular weight: Translated: 15309; Mature: 15309

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTNHPETYDGRQDHIPSLKTPEIESFTNVYEGKDYTIDFTVPEFTAVCPKTGLPDFGVI
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCHHHHCCCCCCCCCCEE
YVSYVPTKRCIELKSFKEYILSYRNVGVFHEFLVNKIMEDLIAAIDPKYLKVIGDYNARG
EEEECCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCHHHEEEECCCCCCC
GIKTVVTREYNKI
CEEEEEECCCCCC
>Mature Secondary Structure
MKTNHPETYDGRQDHIPSLKTPEIESFTNVYEGKDYTIDFTVPEFTAVCPKTGLPDFGVI
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCHHHHCCCCCCCCCCEE
YVSYVPTKRCIELKSFKEYILSYRNVGVFHEFLVNKIMEDLIAAIDPKYLKVIGDYNARG
EEEECCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCHHHEEEECCCCCCC
GIKTVVTREYNKI
CEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA