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The map label for this gene is pcnB [H]

Identifier: 116327797

GI number: 116327797

Start: 1242589

End: 1244058

Strand: Direct

Name: pcnB [H]

Synonym: LBL_1060

Alternate gene names: 116327797

Gene position: 1242589-1244058 (Clockwise)

Preceding gene: 116327794

Following gene: 116327808

Centisome position: 34.38

GC content: 41.77

Gene sequence:

>1470_bases
ATGAAATTCCTGTCCAATCTGTTCAAGAAAAAAATAGGATCAGTTGAGGATATTCTCTCTCACCCGGACGGCAAACGTTA
CTACCGGGATGCCCATCTGATTCGCAAAAATATGATAGATGAGGACGCGGTCAAAATCATCCACAGACTGAATAAATTCG
GCTTCAAGGCTTATATTGTCGGAGGGGGGGTTCGCGACCTTCTTCTCGGGAGGAAGCCGAAAGACTTTGACGTAGTCACT
AACGCGACTCCGAATCAGATCAAAAAAATCTTCAATAATTGTCGAATCATCGGAAGAAGATTCAAAATTGTGCATATACT
TTTCCGCGGAAAAGTGATCGAAGTCAGCACCTTTCGTTCCCTGCCTGATTACAGACTCGGAAAAGCGGTGGAAGATCAAG
ATTACCTCATCAAGAGAGATAACAAGTTCGGTACACCTCAGGAAGACGCCGCTCGCAGAGACTTTACAATCAATTCGTTG
TATTATGATGTAAGAAACGATTCAATCATCGATTATGTCGGCGGTTTTGAGGATATTCGGAATAAAATATTGCGTGTAAT
CGGAGATCCGGATATTTCCTTTCGCGAGGATCCGGTGAGGATGTTGCGTGCAGTGAAGTTTGCGGAAATTCTTGGGCTGG
ACGTAGAAAAAACAACCTCGAAGGCGATTCGCAAACATAAAGCGGAACTGGAAAAGGCTTCCAGTTCAAGAATGTTGGAA
GAATACAATAAGATCTTCCGCACTTGGAAAACTTCTCTGATTTTTCAAGGGATGGCGGAGCATGGTCTTCTCGAAGTTCT
TTTTAAGGAAGCTTTCGACAAGGAAAGAAAGAAGAATTCTTCCTTTGGAGATAAATTCCTCGAAACCAACATCGGCAAAC
GTCTCGCGATCGCTGATAAACTTCTAACCGAAAGGGAGGAGATGACTCCTCATATCTTTTATTCCTTGATTTTTTCCGAT
CTTGCCTCGGAAGCATTAAAAAAAGAGAATATGCATTTGGTTCCAGCGATTAGAAACAGTCTGGAACCGATTTTCAATCG
GCTCGAAACTCCCAAAAAAGATAAGGACCGTTTGATCAAAATTTTCGCAAGTCAGCAAAGGTTCTTAAGCACCGAAGACG
AGAAGTCCTCTCAGAATAATTTCTTCCGTATGAAGGACTATTTTTATGACGCGTTTATGGTTTTTAAAATCGGAGCGATT
GCGGAAAACGATGAAAAAGCGATTCAGAGCGCATTCTTTTGGGAAATTTCCGTAAGAAAAAGACCGATTCCAGTGAAAAG
ATTTAATGGCGGCGGTTCTCGTTCTAATAAAAATCGGAACAAAAATTTCCAGAAGAATCGAGGAGAAGGCGGTCAACGAG
ATCGCCCTAACAGAAGGGAAGAAAATCAAAATTCTTCCGAAAGTCAAAATCAGAGAAATCAAAATGAAGATGATGGAGAG
AACTTTCCCAATCGGGGAAACGAATTTTAA

Upstream 100 bases:

>100_bases
CGTTCGAAATGGGTTAGTTTGAGATTGTACTTTGCCGGACTTGTCATAGATTTTTACTTGCAGGTAAGGGGATCGACGGG
ATTATAGACTCATTCTTTAT

Downstream 100 bases:

>100_bases
GAATCAAAAACTCGTTCCGGTTTTTTGAAAACGAGCTTTAAAGCTCTGGAAAAAACATTTGATTTTTTGAATGTGAAGTT
TGCTCTTCGGTTTCTTGATT

Product: tRNA nucleotidyltransferase

Products: NA

Alternate protein names: PAP; Plasmid copy number protein [H]

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MKFLSNLFKKKIGSVEDILSHPDGKRYYRDAHLIRKNMIDEDAVKIIHRLNKFGFKAYIVGGGVRDLLLGRKPKDFDVVT
NATPNQIKKIFNNCRIIGRRFKIVHILFRGKVIEVSTFRSLPDYRLGKAVEDQDYLIKRDNKFGTPQEDAARRDFTINSL
YYDVRNDSIIDYVGGFEDIRNKILRVIGDPDISFREDPVRMLRAVKFAEILGLDVEKTTSKAIRKHKAELEKASSSRMLE
EYNKIFRTWKTSLIFQGMAEHGLLEVLFKEAFDKERKKNSSFGDKFLETNIGKRLAIADKLLTEREEMTPHIFYSLIFSD
LASEALKKENMHLVPAIRNSLEPIFNRLETPKKDKDRLIKIFASQQRFLSTEDEKSSQNNFFRMKDYFYDAFMVFKIGAI
AENDEKAIQSAFFWEISVRKRPIPVKRFNGGGSRSNKNRNKNFQKNRGEGGQRDRPNRREENQNSSESQNQRNQNEDDGE
NFPNRGNEF

Sequences:

>Translated_489_residues
MKFLSNLFKKKIGSVEDILSHPDGKRYYRDAHLIRKNMIDEDAVKIIHRLNKFGFKAYIVGGGVRDLLLGRKPKDFDVVT
NATPNQIKKIFNNCRIIGRRFKIVHILFRGKVIEVSTFRSLPDYRLGKAVEDQDYLIKRDNKFGTPQEDAARRDFTINSL
YYDVRNDSIIDYVGGFEDIRNKILRVIGDPDISFREDPVRMLRAVKFAEILGLDVEKTTSKAIRKHKAELEKASSSRMLE
EYNKIFRTWKTSLIFQGMAEHGLLEVLFKEAFDKERKKNSSFGDKFLETNIGKRLAIADKLLTEREEMTPHIFYSLIFSD
LASEALKKENMHLVPAIRNSLEPIFNRLETPKKDKDRLIKIFASQQRFLSTEDEKSSQNNFFRMKDYFYDAFMVFKIGAI
AENDEKAIQSAFFWEISVRKRPIPVKRFNGGGSRSNKNRNKNFQKNRGEGGQRDRPNRREENQNSSESQNQRNQNEDDGE
NFPNRGNEF
>Mature_489_residues
MKFLSNLFKKKIGSVEDILSHPDGKRYYRDAHLIRKNMIDEDAVKIIHRLNKFGFKAYIVGGGVRDLLLGRKPKDFDVVT
NATPNQIKKIFNNCRIIGRRFKIVHILFRGKVIEVSTFRSLPDYRLGKAVEDQDYLIKRDNKFGTPQEDAARRDFTINSL
YYDVRNDSIIDYVGGFEDIRNKILRVIGDPDISFREDPVRMLRAVKFAEILGLDVEKTTSKAIRKHKAELEKASSSRMLE
EYNKIFRTWKTSLIFQGMAEHGLLEVLFKEAFDKERKKNSSFGDKFLETNIGKRLAIADKLLTEREEMTPHIFYSLIFSD
LASEALKKENMHLVPAIRNSLEPIFNRLETPKKDKDRLIKIFASQQRFLSTEDEKSSQNNFFRMKDYFYDAFMVFKIGAI
AENDEKAIQSAFFWEISVRKRPIPVKRFNGGGSRSNKNRNKNFQKNRGEGGQRDRPNRREENQNSSESQNQRNQNEDDGE
NFPNRGNEF

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=201, Percent_Identity=32.8358208955224, Blast_Score=85, Evalue=2e-16,
Organism=Escherichia coli, GI87081691, Length=269, Percent_Identity=42.3791821561338, Blast_Score=240, Evalue=1e-64,
Organism=Escherichia coli, GI1789436, Length=226, Percent_Identity=31.4159292035398, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71995920, Length=224, Percent_Identity=29.4642857142857, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281360112, Length=254, Percent_Identity=29.1338582677165, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21357337, Length=254, Percent_Identity=29.1338582677165, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 57089; Mature: 57089

Theoretical pI: Translated: 10.29; Mature: 10.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFLSNLFKKKIGSVEDILSHPDGKRYYRDAHLIRKNMIDEDAVKIIHRLNKFGFKAYIV
CHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCEEEEE
GGGVRDLLLGRKPKDFDVVTNATPNQIKKIFNNCRIIGRRFKIVHILFRGKVIEVSTFRS
CCCHHHHHHCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCEEEEHHHHC
LPDYRLGKAVEDQDYLIKRDNKFGTPQEDAARRDFTINSLYYDVRNDSIIDYVGGFEDIR
CCCHHHCCCCCCCCHHEECCCCCCCCCHHHHHCCCCHHHEEEECCCCCHHHHHCCHHHHH
NKILRVIGDPDISFREDPVRMLRAVKFAEILGLDVEKTTSKAIRKHKAELEKASSSRMLE
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EYNKIFRTWKTSLIFQGMAEHGLLEVLFKEAFDKERKKNSSFGDKFLETNIGKRLAIADK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHH
LLTEREEMTPHIFYSLIFSDLASEALKKENMHLVPAIRNSLEPIFNRLETPKKDKDRLIK
HHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHCCCCCHHHHHHH
IFASQQRFLSTEDEKSSQNNFFRMKDYFYDAFMVFKIGAIAENDEKAIQSAFFWEISVRK
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEEECCC
RPIPVKRFNGGGSRSNKNRNKNFQKNRGEGGQRDRPNRREENQNSSESQNQRNQNEDDGE
CCCCCEECCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCHHCCCCCCHHHHHCCCCCCCCC
NFPNRGNEF
CCCCCCCCC
>Mature Secondary Structure
MKFLSNLFKKKIGSVEDILSHPDGKRYYRDAHLIRKNMIDEDAVKIIHRLNKFGFKAYIV
CHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCEEEEE
GGGVRDLLLGRKPKDFDVVTNATPNQIKKIFNNCRIIGRRFKIVHILFRGKVIEVSTFRS
CCCHHHHHHCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCEEEEHHHHC
LPDYRLGKAVEDQDYLIKRDNKFGTPQEDAARRDFTINSLYYDVRNDSIIDYVGGFEDIR
CCCHHHCCCCCCCCHHEECCCCCCCCCHHHHHCCCCHHHEEEECCCCCHHHHHCCHHHHH
NKILRVIGDPDISFREDPVRMLRAVKFAEILGLDVEKTTSKAIRKHKAELEKASSSRMLE
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EYNKIFRTWKTSLIFQGMAEHGLLEVLFKEAFDKERKKNSSFGDKFLETNIGKRLAIADK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHH
LLTEREEMTPHIFYSLIFSDLASEALKKENMHLVPAIRNSLEPIFNRLETPKKDKDRLIK
HHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCEECHHHHHHHHHHHHHHCCCCCHHHHHHH
IFASQQRFLSTEDEKSSQNNFFRMKDYFYDAFMVFKIGAIAENDEKAIQSAFFWEISVRK
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEEECCC
RPIPVKRFNGGGSRSNKNRNKNFQKNRGEGGQRDRPNRREENQNSSESQNQRNQNEDDGE
CCCCCEECCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCHHCCCCCCHHHHHCCCCCCCCC
NFPNRGNEF
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]