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The map label for this gene is trpB

Identifier: 116327682

GI number: 116327682

Start: 1071640

End: 1072842

Strand: Direct

Name: trpB

Synonym: LBL_0928

Alternate gene names: 116327682

Gene position: 1071640-1072842 (Clockwise)

Preceding gene: 116327681

Following gene: 116327683

Centisome position: 29.65

GC content: 46.38

Gene sequence:

>1203_bases
ATGGGTAAAGTACGTCATTCCCCAAAAGAAGGTTACTTCGGAGAATTCGGCGGTCGCTATTCTCCTGAAATCCTTCACGA
CGCTCTTGCGGAACTTGAGACCACATACAAAAAGCTCAAGAAAAATAAACACTTCAAAAAAGAACTCGAATATTATCGTA
AGAATTATATCGGAAGACCTTCTCCGCTTACATACGCGGAACGTCTCACCAAGGTCTGGGACGGTGCGAGAATCTGGCTC
AAACGAGAAGATTTGAATCATACAGGCGCTCATAAGATCAACAATACGATCGGACAAGTGTTGATCGCAAAGGCGATGGG
CAAAACTCGAATTATTGCGGAAACCGGAGCGGGGCAACACGGGGTTGCGACCGCAACCGTGGGTGCGATGTTTCAGATGG
AAACAGTCGTTTATATGGGAGAAGAGGATCTTCGTCGCCAGGAACTCAACGCGATCCGGATGAGAATGATGGGCGCCAAA
GTCGTAGGTGTTTCAAGCGGAACCGCAACTCTCAAAGACGCTACGAGCGAAGCAATGCGCGATTGGGCATTAAACGTTTC
TAATACACATTATATCGTTGGTTCTTCCATCGGTCCTCATCCGTTTCCTACCATCGTAAGAGACTTTCAATCGGTAATCG
GCATCGAATCCAGAAAACAATTTAAAAAAGTGAACGGTAAACTTCCAAACGCTGTGATCGCCTGTGTCGGCGGCGGTTCG
AACTCGATCGGTATGTTCTACGGTTTCTTAAGGGATAAGAAGGTGAAACTTTTTGGCGTAGAAGCCGGAGGTTATTCTAC
CGAACCTGGGCATCATTCTGCAACAATCCAATTCGGTAGAACCGGGTTTTTACATGGAACCAAAACGTTGGTGATCCAAG
ATGAATTCGGACAAATCGTCCCGGCACATTCCGTGTCTGCAGGTTTGGATTATCCTGGGGTCGGTCCGGAGCACGCTTAT
TTTCATAAAAGCGGGAGAGTGACTTACGTGAACGTGGATGATGACGGTGCTTTAGATGCTTTTCTCGAGATATGTCAGAT
CGAAGGAATCATTCCTGCATTAGAAACTGCTCATGCGTTTCGTTTTGCAAAAGATCTGGCGAAATCGATGGGGAAAAAAG
AAGACATTCTCATCTGCCTTTCCGGAAGAGGAGATAAGGATGTTGCCGAGGTTGCAAGGCTTAGAAAAGGAGAATTTTCT
TGA

Upstream 100 bases:

>100_bases
AAGCTGACAAGGAAGAATCCTTTGTCTTTGCCGGATTCGAGAATTTAATCGCAAGGGTAGAATCTCTGCGTCAAGAACTC
TTTGCAGAATTGGAGTAATT

Downstream 100 bases:

>100_bases
GCGCTATTTCTTCCGTTTTTTCCTCCGATAAAAGCGTTTTCATTCCTTATATCTCTTTGGGAGATCCGGACTACGAGTCT
TGTATTTCTTGGGCGGATGC

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MGKVRHSPKEGYFGEFGGRYSPEILHDALAELETTYKKLKKNKHFKKELEYYRKNYIGRPSPLTYAERLTKVWDGARIWL
KREDLNHTGAHKINNTIGQVLIAKAMGKTRIIAETGAGQHGVATATVGAMFQMETVVYMGEEDLRRQELNAIRMRMMGAK
VVGVSSGTATLKDATSEAMRDWALNVSNTHYIVGSSIGPHPFPTIVRDFQSVIGIESRKQFKKVNGKLPNAVIACVGGGS
NSIGMFYGFLRDKKVKLFGVEAGGYSTEPGHHSATIQFGRTGFLHGTKTLVIQDEFGQIVPAHSVSAGLDYPGVGPEHAY
FHKSGRVTYVNVDDDGALDAFLEICQIEGIIPALETAHAFRFAKDLAKSMGKKEDILICLSGRGDKDVAEVARLRKGEFS

Sequences:

>Translated_400_residues
MGKVRHSPKEGYFGEFGGRYSPEILHDALAELETTYKKLKKNKHFKKELEYYRKNYIGRPSPLTYAERLTKVWDGARIWL
KREDLNHTGAHKINNTIGQVLIAKAMGKTRIIAETGAGQHGVATATVGAMFQMETVVYMGEEDLRRQELNAIRMRMMGAK
VVGVSSGTATLKDATSEAMRDWALNVSNTHYIVGSSIGPHPFPTIVRDFQSVIGIESRKQFKKVNGKLPNAVIACVGGGS
NSIGMFYGFLRDKKVKLFGVEAGGYSTEPGHHSATIQFGRTGFLHGTKTLVIQDEFGQIVPAHSVSAGLDYPGVGPEHAY
FHKSGRVTYVNVDDDGALDAFLEICQIEGIIPALETAHAFRFAKDLAKSMGKKEDILICLSGRGDKDVAEVARLRKGEFS
>Mature_399_residues
GKVRHSPKEGYFGEFGGRYSPEILHDALAELETTYKKLKKNKHFKKELEYYRKNYIGRPSPLTYAERLTKVWDGARIWLK
REDLNHTGAHKINNTIGQVLIAKAMGKTRIIAETGAGQHGVATATVGAMFQMETVVYMGEEDLRRQELNAIRMRMMGAKV
VGVSSGTATLKDATSEAMRDWALNVSNTHYIVGSSIGPHPFPTIVRDFQSVIGIESRKQFKKVNGKLPNAVIACVGGGSN
SIGMFYGFLRDKKVKLFGVEAGGYSTEPGHHSATIQFGRTGFLHGTKTLVIQDEFGQIVPAHSVSAGLDYPGVGPEHAYF
HKSGRVTYVNVDDDGALDAFLEICQIEGIIPALETAHAFRFAKDLAKSMGKKEDILICLSGRGDKDVAEVARLRKGEFS

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family

Homologues:

Organism=Escherichia coli, GI1787515, Length=386, Percent_Identity=50.7772020725389, Blast_Score=402, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6321412, Length=383, Percent_Identity=57.4412532637076, Blast_Score=441, Evalue=1e-124,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPB_LEPBJ (Q04U63)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_800315.1
- ProteinModelPortal:   Q04U63
- SMR:   Q04U63
- STRING:   Q04U63
- GeneID:   4409809
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_0913
- eggNOG:   COG0133
- HOGENOM:   HBG303148
- OMA:   LKREDLC
- PhylomeDB:   Q04U63
- ProtClustDB:   PRK04346
- BioCyc:   LBOR355277:LBJ_0913-MONOMER
- HAMAP:   MF_00133
- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026
- TIGRFAMs:   TIGR00263

Pfam domain/function: PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =4.2.1.20

Molecular weight: Translated: 43998; Mature: 43867

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKVRHSPKEGYFGEFGGRYSPEILHDALAELETTYKKLKKNKHFKKELEYYRKNYIGRP
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCC
SPLTYAERLTKVWDGARIWLKREDLNHTGAHKINNTIGQVLIAKAMGKTRIIAETGAGQH
CCCHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
GVATATVGAMFQMETVVYMGEEDLRRQELNAIRMRMMGAKVVGVSSGTATLKDATSEAMR
CCHHHHHHHHHHHHHEEEECHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHH
DWALNVSNTHYIVGSSIGPHPFPTIVRDFQSVIGIESRKQFKKVNGKLPNAVIACVGGGS
HHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEEECCCC
NSIGMFYGFLRDKKVKLFGVEAGGYSTEPGHHSATIQFGRTGFLHGTKTLVIQDEFGQIV
CCHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCEEEEECCEEEECCCEEEEEECCCCCCC
PAHSVSAGLDYPGVGPEHAYFHKSGRVTYVNVDDDGALDAFLEICQIEGIIPALETAHAF
CCCCCCCCCCCCCCCCCCCEEECCCCEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHH
RFAKDLAKSMGKKEDILICLSGRGDKDVAEVARLRKGEFS
HHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
GKVRHSPKEGYFGEFGGRYSPEILHDALAELETTYKKLKKNKHFKKELEYYRKNYIGRP
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCC
SPLTYAERLTKVWDGARIWLKREDLNHTGAHKINNTIGQVLIAKAMGKTRIIAETGAGQH
CCCHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
GVATATVGAMFQMETVVYMGEEDLRRQELNAIRMRMMGAKVVGVSSGTATLKDATSEAMR
CCHHHHHHHHHHHHHEEEECHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHH
DWALNVSNTHYIVGSSIGPHPFPTIVRDFQSVIGIESRKQFKKVNGKLPNAVIACVGGGS
HHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEEECCCC
NSIGMFYGFLRDKKVKLFGVEAGGYSTEPGHHSATIQFGRTGFLHGTKTLVIQDEFGQIV
CCHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCEEEEECCEEEECCCEEEEEECCCCCCC
PAHSVSAGLDYPGVGPEHAYFHKSGRVTYVNVDDDGALDAFLEICQIEGIIPALETAHAF
CCCCCCCCCCCCCCCCCCCEEECCCCEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHH
RFAKDLAKSMGKKEDILICLSGRGDKDVAEVARLRKGEFS
HHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA