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The map label for this gene is proS [H]

Identifier: 116327681

GI number: 116327681

Start: 1069907

End: 1071637

Strand: Direct

Name: proS [H]

Synonym: LBL_0927

Alternate gene names: 116327681

Gene position: 1069907-1071637 (Clockwise)

Preceding gene: 116327680

Following gene: 116327682

Centisome position: 29.6

GC content: 46.1

Gene sequence:

>1731_bases
ATGAAAGCATCGAAATATATTCTTCCTACAGAAAAGGAAAATCCCTCGGATGCGGTCGTCGCGTCCCATCGTCTTATGAT
CCGCGCCGGTCTTGCGCGCAAGTCCTCCGCAGGTCTTTACTTTTATCTTCCACTCGGACTCAAAATTCTCCAAAAGATCA
AACAGATCATTCGTGAAGAGATGAACAAAACGGGAGCTTTGGAGTTTGATCTTCCGATTTTAACTCCTTCGGATTTCTGG
GAACAAAGCGGTCGGTGGACCGCGATGGGAAAAGAAATGTTTCGTATCAAAGACAGACACGATCTTTCTTATGCCCTCGG
TCCCACACACGAGGAGTCTTTCAGTTTTTTATTAAAACCTCTTTTGAAATCATATAAGGATCTTCCGCTCAACGTCTATC
AGATCCAAACTAAGTTCCGGGACGAAATTCGTCCTCGTTTCGGTGTGATTCGTTCGAGGGAGTTCATTATGAAAGATGCA
TATTCCTTTCATATCGACGATGTTTCTCTCGACGAAACGTATCAATCGATGCGGGCCGCTTATAGAAAGATTTTCGATCG
CTGCGGTCTCAAGACGATTCCCGTGCAGGCTGATTCCGGAAGTATGGGCGGTTCCGCTTCGGAGGAATTTATGGTGGTTT
CTCCAATTGGAGAAGAAACGTTGCTTTTATGTAATTCCTGCGGATACAGTTCCAACAGCGAAAAAACCCCCCTTGTATTA
AAAAAAGAGAATGTATCTTCGGCGTCCGTCGAGAAGAAAGAAATCTCCACTCCGGGCAAAAAGACGATCGTCGAGGTCAG
CGCTTTTTTGGGAATCCCCGAATCGACGACGATCAAGGCCGCCACGCTCAAATCGGAAAAGAAAAAGATTCTCGTTTATT
TGCGCGGGGATCTCGAACTCAATCTTCACAAACTTCATTCTTTGCTGAGAATCGTGGATTCCGAGCCGATGACTGACGCG
GAAATCCGCGAACTCGGACTCGTTCCCGGTTTTATCGCGCCCGTCGCACCGAACGATAAAGTGAAAGTGTTGTATGACCG
TTCCCTGCAAAAGGATTTTCCATATGTGGTCGCTTCCAACAAAGAGGACTTTCACACACAAGGTTTCGTTCTCGAAAAGG
AAGTTTCCGGTCTTCCGGAGTTCGCCGATGTCGCATTAGCAAGAGAAGGGGATCTTTGTCCGAATTGCAACGCTCCTTTA
AAGGCCGAAAAAGGGATCGAGGTTGGTCATATATTCAAACTCGGCGAAAAATACACAAAGGCGTTCGGAATTCAAGTCCT
TGATCAAAACGGAAAAGCCAGAACGCTTACGATGGGTTGTTACGGGATCGGTGTTAATAGAACCATGGCGACCGTCATCG
AACAACGCAACGACGAGAAAGGGATCTTTTGGCCGATCAGCATCGCACCTTTCGAAGTGACTCTTGTGAGCATCACCAAA
GGCGAGGAACAATATTCCAAAGCGGAAGAATTTTATAATGTTCTAAAGAACGAAAACTTAGAAGTATTCTGGGACGACCG
TGACGTGGGACCGGGCTTCAAACTCAAGGATTCCGAACTGATCGGATTTCCGATCCGAGTCACGATCGGTAAAAAATTCT
TCGAAAACGGAGAGATTTCGATCTACAACCGCAAAGCTGACAAGGAAGAATCCTTTGTCTTTGCCGGATTCGAGAATTTA
ATCGCAAGGGTAGAATCTCTGCGTCAAGAACTCTTTGCAGAATTGGAGTAA

Upstream 100 bases:

>100_bases
GGTAGAGTAATCGAGTCCATCTTCCGAATCGGATTTTTGTTTTTACTCGGACTCGGACTCTATGTCACTTTCAACGATGT
AATGCGAATTTTCTAAACGC

Downstream 100 bases:

>100_bases
TTATGGGTAAAGTACGTCATTCCCCAAAAGAAGGTTACTTCGGAGAATTCGGCGGTCGCTATTCTCCTGAAATCCTTCAC
GACGCTCTTGCGGAACTTGA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 576; Mature: 576

Protein sequence:

>576_residues
MKASKYILPTEKENPSDAVVASHRLMIRAGLARKSSAGLYFYLPLGLKILQKIKQIIREEMNKTGALEFDLPILTPSDFW
EQSGRWTAMGKEMFRIKDRHDLSYALGPTHEESFSFLLKPLLKSYKDLPLNVYQIQTKFRDEIRPRFGVIRSREFIMKDA
YSFHIDDVSLDETYQSMRAAYRKIFDRCGLKTIPVQADSGSMGGSASEEFMVVSPIGEETLLLCNSCGYSSNSEKTPLVL
KKENVSSASVEKKEISTPGKKTIVEVSAFLGIPESTTIKAATLKSEKKKILVYLRGDLELNLHKLHSLLRIVDSEPMTDA
EIRELGLVPGFIAPVAPNDKVKVLYDRSLQKDFPYVVASNKEDFHTQGFVLEKEVSGLPEFADVALAREGDLCPNCNAPL
KAEKGIEVGHIFKLGEKYTKAFGIQVLDQNGKARTLTMGCYGIGVNRTMATVIEQRNDEKGIFWPISIAPFEVTLVSITK
GEEQYSKAEEFYNVLKNENLEVFWDDRDVGPGFKLKDSELIGFPIRVTIGKKFFENGEISIYNRKADKEESFVFAGFENL
IARVESLRQELFAELE

Sequences:

>Translated_576_residues
MKASKYILPTEKENPSDAVVASHRLMIRAGLARKSSAGLYFYLPLGLKILQKIKQIIREEMNKTGALEFDLPILTPSDFW
EQSGRWTAMGKEMFRIKDRHDLSYALGPTHEESFSFLLKPLLKSYKDLPLNVYQIQTKFRDEIRPRFGVIRSREFIMKDA
YSFHIDDVSLDETYQSMRAAYRKIFDRCGLKTIPVQADSGSMGGSASEEFMVVSPIGEETLLLCNSCGYSSNSEKTPLVL
KKENVSSASVEKKEISTPGKKTIVEVSAFLGIPESTTIKAATLKSEKKKILVYLRGDLELNLHKLHSLLRIVDSEPMTDA
EIRELGLVPGFIAPVAPNDKVKVLYDRSLQKDFPYVVASNKEDFHTQGFVLEKEVSGLPEFADVALAREGDLCPNCNAPL
KAEKGIEVGHIFKLGEKYTKAFGIQVLDQNGKARTLTMGCYGIGVNRTMATVIEQRNDEKGIFWPISIAPFEVTLVSITK
GEEQYSKAEEFYNVLKNENLEVFWDDRDVGPGFKLKDSELIGFPIRVTIGKKFFENGEISIYNRKADKEESFVFAGFENL
IARVESLRQELFAELE
>Mature_576_residues
MKASKYILPTEKENPSDAVVASHRLMIRAGLARKSSAGLYFYLPLGLKILQKIKQIIREEMNKTGALEFDLPILTPSDFW
EQSGRWTAMGKEMFRIKDRHDLSYALGPTHEESFSFLLKPLLKSYKDLPLNVYQIQTKFRDEIRPRFGVIRSREFIMKDA
YSFHIDDVSLDETYQSMRAAYRKIFDRCGLKTIPVQADSGSMGGSASEEFMVVSPIGEETLLLCNSCGYSSNSEKTPLVL
KKENVSSASVEKKEISTPGKKTIVEVSAFLGIPESTTIKAATLKSEKKKILVYLRGDLELNLHKLHSLLRIVDSEPMTDA
EIRELGLVPGFIAPVAPNDKVKVLYDRSLQKDFPYVVASNKEDFHTQGFVLEKEVSGLPEFADVALAREGDLCPNCNAPL
KAEKGIEVGHIFKLGEKYTKAFGIQVLDQNGKARTLTMGCYGIGVNRTMATVIEQRNDEKGIFWPISIAPFEVTLVSITK
GEEQYSKAEEFYNVLKNENLEVFWDDRDVGPGFKLKDSELIGFPIRVTIGKKFFENGEISIYNRKADKEESFVFAGFENL
IARVESLRQELFAELE

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=224, Percent_Identity=41.5178571428571, Blast_Score=202, Evalue=1e-51,
Organism=Escherichia coli, GI1786392, Length=560, Percent_Identity=43.2142857142857, Blast_Score=461, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI115532348, Length=232, Percent_Identity=39.2241379310345, Blast_Score=166, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI193203271, Length=99, Percent_Identity=35.3535353535354, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320931, Length=545, Percent_Identity=30.2752293577982, Blast_Score=246, Evalue=6e-66,
Organism=Drosophila melanogaster, GI24656200, Length=243, Percent_Identity=37.8600823045267, Blast_Score=183, Evalue=3e-46,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 64952; Mature: 64952

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASKYILPTEKENPSDAVVASHRLMIRAGLARKSSAGLYFYLPLGLKILQKIKQIIREE
CCCCCCCCCCCCCCCCCHHHHHHHHEEEECCCCCCCCCEEEEECHHHHHHHHHHHHHHHH
MNKTGALEFDLPILTPSDFWEQSGRWTAMGKEMFRIKDRHDLSYALGPTHEESFSFLLKP
HCCCCCEEEECEECCCHHHHHCCCCEEECCHHHHHCCCCCCCEEECCCCCHHHHHHHHHH
LLKSYKDLPLNVYQIQTKFRDEIRPRFGVIRSREFIMKDAYSFHIDDVSLDETYQSMRAA
HHHHHCCCCCEEEEEHHHHHHHHCHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHH
YRKIFDRCGLKTIPVQADSGSMGGSASEEFMVVSPIGEETLLLCNSCGYSSNSEKTPLVL
HHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCCEEE
KKENVSSASVEKKEISTPGKKTIVEVSAFLGIPESTTIKAATLKSEKKKILVYLRGDLEL
EECCCCCCCCCHHHCCCCCHHHHEEEHHHHCCCCCCEEEEEHCCCCCCEEEEEEECCCEE
NLHKLHSLLRIVDSEPMTDAEIRELGLVPGFIAPVAPNDKVKVLYDRSLQKDFPYVVASN
CHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEECC
KEDFHTQGFVLEKEVSGLPEFADVALAREGDLCPNCNAPLKAEKGIEVGHIFKLGEKYTK
CCCCCCCCEEEEHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
AFGIQVLDQNGKARTLTMGCYGIGVNRTMATVIEQRNDEKGIFWPISIAPFEVTLVSITK
HHCEEEECCCCCEEEEEEEEEECCCCHHHHHHHHHCCCCCCEEEEEEECCEEEEEEEEEC
GEEQYSKAEEFYNVLKNENLEVFWDDRDVGPGFKLKDSELIGFPIRVTIGKKFFENGEIS
CHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEECCCCEEEEEEEEEECHHHHCCCCEE
IYNRKADKEESFVFAGFENLIARVESLRQELFAELE
EEECCCCCCCCEEEECHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKASKYILPTEKENPSDAVVASHRLMIRAGLARKSSAGLYFYLPLGLKILQKIKQIIREE
CCCCCCCCCCCCCCCCCHHHHHHHHEEEECCCCCCCCCEEEEECHHHHHHHHHHHHHHHH
MNKTGALEFDLPILTPSDFWEQSGRWTAMGKEMFRIKDRHDLSYALGPTHEESFSFLLKP
HCCCCCEEEECEECCCHHHHHCCCCEEECCHHHHHCCCCCCCEEECCCCCHHHHHHHHHH
LLKSYKDLPLNVYQIQTKFRDEIRPRFGVIRSREFIMKDAYSFHIDDVSLDETYQSMRAA
HHHHHCCCCCEEEEEHHHHHHHHCHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHH
YRKIFDRCGLKTIPVQADSGSMGGSASEEFMVVSPIGEETLLLCNSCGYSSNSEKTPLVL
HHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCCEEE
KKENVSSASVEKKEISTPGKKTIVEVSAFLGIPESTTIKAATLKSEKKKILVYLRGDLEL
EECCCCCCCCCHHHCCCCCHHHHEEEHHHHCCCCCCEEEEEHCCCCCCEEEEEEECCCEE
NLHKLHSLLRIVDSEPMTDAEIRELGLVPGFIAPVAPNDKVKVLYDRSLQKDFPYVVASN
CHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEECC
KEDFHTQGFVLEKEVSGLPEFADVALAREGDLCPNCNAPLKAEKGIEVGHIFKLGEKYTK
CCCCCCCCEEEEHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
AFGIQVLDQNGKARTLTMGCYGIGVNRTMATVIEQRNDEKGIFWPISIAPFEVTLVSITK
HHCEEEECCCCCEEEEEEEEEECCCCHHHHHHHHHCCCCCCEEEEEEECCEEEEEEEEEC
GEEQYSKAEEFYNVLKNENLEVFWDDRDVGPGFKLKDSELIGFPIRVTIGKKFFENGEIS
CHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEECCCCEEEEEEEEEECHHHHCCCCEE
IYNRKADKEESFVFAGFENLIARVESLRQELFAELE
EEECCCCCCCCEEEECHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA