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The map label for this gene is folC [H]

Identifier: 116327549

GI number: 116327549

Start: 885506

End: 886804

Strand: Reverse

Name: folC [H]

Synonym: LBL_0778

Alternate gene names: 116327549

Gene position: 886804-885506 (Counterclockwise)

Preceding gene: 116327550

Following gene: 116327540

Centisome position: 24.53

GC content: 40.34

Gene sequence:

>1299_bases
ATGAGTGAAGATTCCGACTTTTTCAAATTTATCAACCAACTTCCCAACTTAGAGAAAACCAGAAACTTTAACGTTTTTAC
CGGATATTCTCTCGAACCGTTCGCAAAGGTTTTGAATCAATTCGACTTGAACAAAAGAAACCGATCCACTCTTTGCAGAA
TTTCCGTGATAGGAACAAATGCGAAGGGTTCCATCGCACATTTTTTAGGAGAATACTTCAGACTTTTCGGTTTCAAAACA
GGGCTTTATACCTCGCCACACTTGATCTCTCCTTTGGAAAGGATTAAAATCGGCACAAATGCGCAAAACTTCCGGGATAT
TCAAACGAAAGAATTGGATCGATTGTTAAACGAATGGAAATCCATAGGAGCGGAAGAAGCTTTGAAATCCTTTTCTTTTT
TCGAACTCTTTACCTGTGCCGCCTTCTTTTTTTTCGAAAAAGAATTTGCGGAAATCCAAATTTACGAAGCAGGACTCGGA
GGAAGGCTGGATGCAACAAAACTCTGTGATCCGGACGTAGTCGTTTTGGGAGTTATAGGTTTAGACCACAGGGAAATATT
AGGAAATACGAAAGAAGAAATCCTGTACGAAAAACTAAAGATCTGCACCACCAACACAAAAGCCTTTTTTGCGATCGAAC
AGGAAGAACCAAACTTAAACGAAAAAATCAAATCCTGGTGTTCTCAAAATAAGATTTCTTGCACGATTCTGCCGCAGATT
CCGAGCGATGCGACCTATCTCATCAGGAATCAACATTTCTCTTATCAAGTATTTCAGAATATTTTAAATTCTGAATTGTT
CCAAAAATTAAAACGCCCGACGATAGATCCGGGATTCTCTAAACCTGAAAACGTTTTTTTGGCTTCAAACGCGCCCGGTC
TCAAAACTTTCGAAAAATACATAACCCTTCCGTCGGGAAGATTAAGCGTTTTACGAAATTCCCCCCTACTCGTTTTCGAT
CCTGCACACAATCCGGACGCCGTTAAAGAAACTCTGCAAAGTTTATCCATTCTGTATCCTGGGCAAAAGTTTCAGGTTTA
TGTCGGTTTGTTGAAGGATAAGGATGGGGACGGAATCTTGAAAATTCTGCGGAATGCGCTTAACCAATCGGATATTTTCG
ACTTTCAGTTTTTAAAGGAGGACGGGTTCGCTCTTCCTGAAAGTTGTCGCTTCAAAGAGACAATCGACGGCGCCGAGTTC
AGATCCATCTTACAAAATTCAGACGATATACAAAAGTCGACTTTAATTCTCGGGAGCTTTCGTTTATTCCCGATTGTTTC
GGATTCGATCTGTCATTAA

Upstream 100 bases:

>100_bases
ATCAGGCGATGAAACAGTTCGAACTTTTCACGGGTATTTCGCCTAATGCAAGCGATATCCTCAAAACCAGAGAACGTTTG
TCTCAAACATTAACAAATCA

Downstream 100 bases:

>100_bases
ATCTATTCTCAAAACGTAGGAACTACCGCAGATCACAATCTTACGAACACATTCAAAAGAGTCTGTCCAAAACTGTTTTC
AGTAGGAGCGCGCGACACTG

Product: bifunctional folylpolyglutamate synthase/dihydrofolate synthase

Products: NA

Alternate protein names: Folylpolyglutamate synthase; Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase; Dihydrofolate synthase [H]

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MSEDSDFFKFINQLPNLEKTRNFNVFTGYSLEPFAKVLNQFDLNKRNRSTLCRISVIGTNAKGSIAHFLGEYFRLFGFKT
GLYTSPHLISPLERIKIGTNAQNFRDIQTKELDRLLNEWKSIGAEEALKSFSFFELFTCAAFFFFEKEFAEIQIYEAGLG
GRLDATKLCDPDVVVLGVIGLDHREILGNTKEEILYEKLKICTTNTKAFFAIEQEEPNLNEKIKSWCSQNKISCTILPQI
PSDATYLIRNQHFSYQVFQNILNSELFQKLKRPTIDPGFSKPENVFLASNAPGLKTFEKYITLPSGRLSVLRNSPLLVFD
PAHNPDAVKETLQSLSILYPGQKFQVYVGLLKDKDGDGILKILRNALNQSDIFDFQFLKEDGFALPESCRFKETIDGAEF
RSILQNSDDIQKSTLILGSFRLFPIVSDSICH

Sequences:

>Translated_432_residues
MSEDSDFFKFINQLPNLEKTRNFNVFTGYSLEPFAKVLNQFDLNKRNRSTLCRISVIGTNAKGSIAHFLGEYFRLFGFKT
GLYTSPHLISPLERIKIGTNAQNFRDIQTKELDRLLNEWKSIGAEEALKSFSFFELFTCAAFFFFEKEFAEIQIYEAGLG
GRLDATKLCDPDVVVLGVIGLDHREILGNTKEEILYEKLKICTTNTKAFFAIEQEEPNLNEKIKSWCSQNKISCTILPQI
PSDATYLIRNQHFSYQVFQNILNSELFQKLKRPTIDPGFSKPENVFLASNAPGLKTFEKYITLPSGRLSVLRNSPLLVFD
PAHNPDAVKETLQSLSILYPGQKFQVYVGLLKDKDGDGILKILRNALNQSDIFDFQFLKEDGFALPESCRFKETIDGAEF
RSILQNSDDIQKSTLILGSFRLFPIVSDSICH
>Mature_431_residues
SEDSDFFKFINQLPNLEKTRNFNVFTGYSLEPFAKVLNQFDLNKRNRSTLCRISVIGTNAKGSIAHFLGEYFRLFGFKTG
LYTSPHLISPLERIKIGTNAQNFRDIQTKELDRLLNEWKSIGAEEALKSFSFFELFTCAAFFFFEKEFAEIQIYEAGLGG
RLDATKLCDPDVVVLGVIGLDHREILGNTKEEILYEKLKICTTNTKAFFAIEQEEPNLNEKIKSWCSQNKISCTILPQIP
SDATYLIRNQHFSYQVFQNILNSELFQKLKRPTIDPGFSKPENVFLASNAPGLKTFEKYITLPSGRLSVLRNSPLLVFDP
AHNPDAVKETLQSLSILYPGQKFQVYVGLLKDKDGDGILKILRNALNQSDIFDFQFLKEDGFALPESCRFKETIDGAEFR
SILQNSDDIQKSTLILGSFRLFPIVSDSICH

Specific function: Conversion of folates to polyglutamate derivatives [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=397, Percent_Identity=25.1889168765743, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI66932990, Length=342, Percent_Identity=26.0233918128655, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1788654, Length=393, Percent_Identity=25.1908396946565, Blast_Score=120, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71984923, Length=177, Percent_Identity=30.5084745762712, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17553148, Length=177, Percent_Identity=30.5084745762712, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17553150, Length=177, Percent_Identity=30.5084745762712, Blast_Score=82, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6323760, Length=345, Percent_Identity=25.2173913043478, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6324815, Length=196, Percent_Identity=32.1428571428571, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24641571, Length=219, Percent_Identity=28.7671232876712, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24581568, Length=187, Percent_Identity=26.2032085561497, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17; =6.3.2.12 [H]

Molecular weight: Translated: 49200; Mature: 49069

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEDSDFFKFINQLPNLEKTRNFNVFTGYSLEPFAKVLNQFDLNKRNRSTLCRISVIGTN
CCCCHHHHHHHHHCCCCHHHCCCCEEECCCHHHHHHHHHHHCCCCCCCCEEEEEEEEECC
AKGSIAHFLGEYFRLFGFKTGLYTSPHLISPLERIKIGTNAQNFRDIQTKELDRLLNEWK
CCCHHHHHHHHHHHHHHCCCCCCCCCHHHCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
SIGAEEALKSFSFFELFTCAAFFFFEKEFAEIQIYEAGLGGRLDATKLCDPDVVVLGVIG
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCCEEEEEEEC
LDHREILGNTKEEILYEKLKICTTNTKAFFAIEQEEPNLNEKIKSWCSQNKISCTILPQI
CCHHHHHCCCHHHHHHHHHHHEECCCEEEEEEECCCCCHHHHHHHHHCCCCEEEEEECCC
PSDATYLIRNQHFSYQVFQNILNSELFQKLKRPTIDPGFSKPENVFLASNAPGLKTFEKY
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCHHHHHHH
ITLPSGRLSVLRNSPLLVFDPAHNPDAVKETLQSLSILYPGQKFQVYVGLLKDKDGDGIL
HCCCCCHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCHHH
KILRNALNQSDIFDFQFLKEDGFALPESCRFKETIDGAEFRSILQNSDDIQKSTLILGSF
HHHHHHCCCCCCCHHEEECCCCCCCCCCCCHHHHCCHHHHHHHHCCCCCHHHHHEEEECE
RLFPIVSDSICH
EEEEECCCCCCC
>Mature Secondary Structure 
SEDSDFFKFINQLPNLEKTRNFNVFTGYSLEPFAKVLNQFDLNKRNRSTLCRISVIGTN
CCCHHHHHHHHHCCCCHHHCCCCEEECCCHHHHHHHHHHHCCCCCCCCEEEEEEEEECC
AKGSIAHFLGEYFRLFGFKTGLYTSPHLISPLERIKIGTNAQNFRDIQTKELDRLLNEWK
CCCHHHHHHHHHHHHHHCCCCCCCCCHHHCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
SIGAEEALKSFSFFELFTCAAFFFFEKEFAEIQIYEAGLGGRLDATKLCDPDVVVLGVIG
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCCEEEEEEEC
LDHREILGNTKEEILYEKLKICTTNTKAFFAIEQEEPNLNEKIKSWCSQNKISCTILPQI
CCHHHHHCCCHHHHHHHHHHHEECCCEEEEEEECCCCCHHHHHHHHHCCCCEEEEEECCC
PSDATYLIRNQHFSYQVFQNILNSELFQKLKRPTIDPGFSKPENVFLASNAPGLKTFEKY
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCHHHHHHH
ITLPSGRLSVLRNSPLLVFDPAHNPDAVKETLQSLSILYPGQKFQVYVGLLKDKDGDGIL
HCCCCCHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCHHH
KILRNALNQSDIFDFQFLKEDGFALPESCRFKETIDGAEFRSILQNSDDIQKSTLILGSF
HHHHHHCCCCCCCHHEEECCCCCCCCCCCCHHHHCCHHHHHHHHCCCCCHHHHHEEEECE
RLFPIVSDSICH
EEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12089438 [H]