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The map label for this gene is glmU [H]

Identifier: 116327332

GI number: 116327332

Start: 578868

End: 579626

Strand: Reverse

Name: glmU [H]

Synonym: LBL_0526

Alternate gene names: 116327332

Gene position: 579626-578868 (Counterclockwise)

Preceding gene: 116327333

Following gene: 116327331

Centisome position: 16.04

GC content: 44.8

Gene sequence:

>759_bases
ATGAAACCTACTCAGGATAAGGTCGCTGTGGTATTGGCCGCAGGGAAGGGCACCCGTATGAAAACGGATCAGCCTAAAGT
TGCGGTAGAGCTGAATGGCAAACCGCTACTTCTCCATGTACTCGATCATCTGAAAGGCTCCGGCATAGAACGAATCGTAG
TCGTAGTAGGTTATAAAAAAGAATTGGTCCAGGCGCTTTGCTCTGAAATATCCGGAGTCTCCTTCGTTGAACAAAAAGAA
CAACTGGGAACCGCCCACGCACTACTTTGCGCGGAACCCGAACTCAAAAACTTTCAAGGTTCCGTAATCGTCGCCTGTGG
CGACGTTCCTATGATCACTTCCAAAACGTTTGCCGATATCGTAAAAGAACATAGAGAGAATGAATTCTCCGCTACGATTC
TTTCCGCGGTCGTAGAAAAACCGACAGGTTATGGAAGAATCATTCGCAACGCATCGGGTGATGTTACCGCTATCGTCGAA
GAAAAGGATTCTTCTTCCGAAGAAAAATTGATCAACGAAATCAATACCGGAACCTACGTCTTCGAAGGAGAAGATCTTTT
CGATTCTCTGAAAAAAATCGGAAATCAAAACGCTCAGGGAGAATATTATCTTCCCGACTTAGTGAAATTATATAGAAACT
CCGGAAAAAAACTCGGAGCTATGAAGTTGAAGAATCACTTGGAAAGTCACGGAGTGAATTCCCCCGAAGACCTACATATA
CTTTCCTCTATGATCAAGGGAGAGGCTGTCCATCCATGA

Upstream 100 bases:

>100_bases
TGGGCTGTCGCCAAGTGGTAAGGCAGCGGTTTTTGGTATCGCCATTTCCTAGGTTCGAATCCTAGCAGCCCAGCCATTGA
ATTACTGACATTGATTAATG

Downstream 100 bases:

>100_bases
ACGGAGACATCGCGGTATTTGCGGGAAGTTCCAATAAACAGATTGCAGAAGAAATCTGTACTCACCTAAACATTCAACCG
GGTAAGATTAACCTAAAAAA

Product: UDP-N-acetylglucosamine diphosphorylase

Products: NA

Alternate protein names: UDP-N-acetylglucosamine pyrophosphorylase; N-acetylglucosamine-1-phosphate uridyltransferase; Glucosamine-1-phosphate N-acetyltransferase [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MKPTQDKVAVVLAAGKGTRMKTDQPKVAVELNGKPLLLHVLDHLKGSGIERIVVVVGYKKELVQALCSEISGVSFVEQKE
QLGTAHALLCAEPELKNFQGSVIVACGDVPMITSKTFADIVKEHRENEFSATILSAVVEKPTGYGRIIRNASGDVTAIVE
EKDSSSEEKLINEINTGTYVFEGEDLFDSLKKIGNQNAQGEYYLPDLVKLYRNSGKKLGAMKLKNHLESHGVNSPEDLHI
LSSMIKGEAVHP

Sequences:

>Translated_252_residues
MKPTQDKVAVVLAAGKGTRMKTDQPKVAVELNGKPLLLHVLDHLKGSGIERIVVVVGYKKELVQALCSEISGVSFVEQKE
QLGTAHALLCAEPELKNFQGSVIVACGDVPMITSKTFADIVKEHRENEFSATILSAVVEKPTGYGRIIRNASGDVTAIVE
EKDSSSEEKLINEINTGTYVFEGEDLFDSLKKIGNQNAQGEYYLPDLVKLYRNSGKKLGAMKLKNHLESHGVNSPEDLHI
LSSMIKGEAVHP
>Mature_252_residues
MKPTQDKVAVVLAAGKGTRMKTDQPKVAVELNGKPLLLHVLDHLKGSGIERIVVVVGYKKELVQALCSEISGVSFVEQKE
QLGTAHALLCAEPELKNFQGSVIVACGDVPMITSKTFADIVKEHRENEFSATILSAVVEKPTGYGRIIRNASGDVTAIVE
EKDSSSEEKLINEINTGTYVFEGEDLFDSLKKIGNQNAQGEYYLPDLVKLYRNSGKKLGAMKLKNHLESHGVNSPEDLHI
LSSMIKGEAVHP

Specific function: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-GlcNAc. Responsible for the acetylation of Glc-N-1-P to give GlcNAc-1-P and for the uridyl transfer from UTP to GlcNAc-1-P which produces UDP-GlcNAc [H]

COG id: COG1207

COG function: function code M; N-acetylglucosamine-1-phosphate uridyltransferase (contains nucleotidyltransferase and I-patch acetyltransferase domains)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Homo sapiens, GI11761621, Length=185, Percent_Identity=29.1891891891892, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI11761619, Length=185, Percent_Identity=29.1891891891892, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1790168, Length=240, Percent_Identity=37.9166666666667, Blast_Score=145, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI133931050, Length=187, Percent_Identity=29.4117647058824, Blast_Score=71, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6320148, Length=184, Percent_Identity=27.1739130434783, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI21355443, Length=224, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24644084, Length=224, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005882
- InterPro:   IPR018357
- InterPro:   IPR005835
- InterPro:   IPR011004 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.23; =2.3.1.157 [H]

Molecular weight: Translated: 27437; Mature: 27437

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPTQDKVAVVLAAGKGTRMKTDQPKVAVELNGKPLLLHVLDHLKGSGIERIVVVVGYKK
CCCCCCEEEEEEECCCCCCCCCCCCCEEEEECCCEEHHHHHHHHCCCCCEEEEEEECCHH
ELVQALCSEISGVSFVEQKEQLGTAHALLCAEPELKNFQGSVIVACGDVPMITSKTFADI
HHHHHHHHHHCCCHHHHHHHHHCHHHEEEECCCCCCCCCCCEEEEECCCCCCCHHHHHHH
VKEHRENEFSATILSAVVEKPTGYGRIIRNASGDVTAIVEEKDSSSEEKLINEINTGTYV
HHHHCCCHHHHHHHHHHHHCCCCCHHHEECCCCCEEEEEECCCCCHHHHHHHHHCCCEEE
FEGEDLFDSLKKIGNQNAQGEYYLPDLVKLYRNSGKKLGAMKLKNHLESHGVNSPEDLHI
EECHHHHHHHHHHCCCCCCCCEECHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHH
LSSMIKGEAVHP
HHHHHCCCCCCC
>Mature Secondary Structure
MKPTQDKVAVVLAAGKGTRMKTDQPKVAVELNGKPLLLHVLDHLKGSGIERIVVVVGYKK
CCCCCCEEEEEEECCCCCCCCCCCCCEEEEECCCEEHHHHHHHHCCCCCEEEEEEECCHH
ELVQALCSEISGVSFVEQKEQLGTAHALLCAEPELKNFQGSVIVACGDVPMITSKTFADI
HHHHHHHHHHCCCHHHHHHHHHCHHHEEEECCCCCCCCCCCEEEEECCCCCCCHHHHHHH
VKEHRENEFSATILSAVVEKPTGYGRIIRNASGDVTAIVEEKDSSSEEKLINEINTGTYV
HHHHCCCHHHHHHHHHHHHCCCCCHHHEECCCCCEEEEEECCCCCHHHHHHHHHCCCEEE
FEGEDLFDSLKKIGNQNAQGEYYLPDLVKLYRNSGKKLGAMKLKNHLESHGVNSPEDLHI
EECHHHHHHHHHHCCCCCCCCEECHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHH
LSSMIKGEAVHP
HHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]