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The map label for this gene is prsA [H]

Identifier: 116327331

GI number: 116327331

Start: 577933

End: 578871

Strand: Reverse

Name: prsA [H]

Synonym: LBL_0525

Alternate gene names: 116327331

Gene position: 578871-577933 (Counterclockwise)

Preceding gene: 116327332

Following gene: 116327330

Centisome position: 16.02

GC content: 46.33

Gene sequence:

>939_bases
ATGAACGGAGACATCGCGGTATTTGCGGGAAGTTCCAATAAACAGATTGCAGAAGAAATCTGTACTCACCTAAACATTCA
ACCGGGTAAGATTAACCTAAAAAAATTCTCCGACGGAGAAATTTCAGTTAAAGTGGAAGACAACGTTCGAGGAAGAGAAG
TGTTCATCGTTCAATCCACTTCCGCTCCGGCTAACGATCATTTGATGGAATTAATCCTGATCATGGACGCATTCCGCAGA
GCCTCCGTATCCAGCATCAGCGTCGTGATTCCTTATTACGGTTACGGACGTCAAGACAGAAAGGTGGAGCCTCGTGTTCC
TATTTCCGCGAGAATTGTAGCGGATCTTCTGGAAGTTGTGGGTCTCGACAGAATTCTTACGATGGATTTACACGCGGATC
AGATTCAAGGATTCTTTCGCGTCCCCGTCGATAATCTTCACTTTGCTCCGGTTTTAGCGGAATACGTCAATACGAAAAAC
ATCAATGACCTTGTAATCGTTTCCCCCGATTCGGGCGGAGCGGAAAGAGCGAGAGCTTTCGGGAAAAAAGTAAACGGTTC
ATTAGCGATTATTGATAAACGAAGACCGAAAGCGAACGTCTCCGAAGTGATGAACGTGATCGGAGAAATCGAAGGCAAGA
ATTGTATTCTTCTCGACGACATGATCGACACGGCCGGAACCATCTGCAAGGCCGCGGATGCTCTGTTAAAACATGGAGCC
AAGTCCGTTTATTGCGCCGCGACTCACGGAGTACTTTCCGGCGAATCGGTGGATCGGATCAACGCGACGAACTTCACGGA
AGTTGTTCTTGCGAACACGATCGCGATTCCAGAATCCAAGAAGATTCACAAACTGAAATCATTGTCCGTAGCTCCTTTGT
TCGCGAACGCGATCAAAAGGATTCATACAAATCAATCAGTCAGCACTTTATTCGATTAA

Upstream 100 bases:

>100_bases
GAGCTATGAAGTTGAAGAATCACTTGGAAAGTCACGGAGTGAATTCCCCCGAAGACCTACATATACTTTCCTCTATGATC
AAGGGAGAGGCTGTCCATCC

Downstream 100 bases:

>100_bases
GGTTAGGTAATAAGAATGAGCCAGAGCACAATTCACAAAATCGCGGTTAAAAAGAGAACGGAAACCGGTAAAAACGAAAA
CAACCGTCTTCGTTCTTCGG

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase [H]

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MNGDIAVFAGSSNKQIAEEICTHLNIQPGKINLKKFSDGEISVKVEDNVRGREVFIVQSTSAPANDHLMELILIMDAFRR
ASVSSISVVIPYYGYGRQDRKVEPRVPISARIVADLLEVVGLDRILTMDLHADQIQGFFRVPVDNLHFAPVLAEYVNTKN
INDLVIVSPDSGGAERARAFGKKVNGSLAIIDKRRPKANVSEVMNVIGEIEGKNCILLDDMIDTAGTICKAADALLKHGA
KSVYCAATHGVLSGESVDRINATNFTEVVLANTIAIPESKKIHKLKSLSVAPLFANAIKRIHTNQSVSTLFD

Sequences:

>Translated_312_residues
MNGDIAVFAGSSNKQIAEEICTHLNIQPGKINLKKFSDGEISVKVEDNVRGREVFIVQSTSAPANDHLMELILIMDAFRR
ASVSSISVVIPYYGYGRQDRKVEPRVPISARIVADLLEVVGLDRILTMDLHADQIQGFFRVPVDNLHFAPVLAEYVNTKN
INDLVIVSPDSGGAERARAFGKKVNGSLAIIDKRRPKANVSEVMNVIGEIEGKNCILLDDMIDTAGTICKAADALLKHGA
KSVYCAATHGVLSGESVDRINATNFTEVVLANTIAIPESKKIHKLKSLSVAPLFANAIKRIHTNQSVSTLFD
>Mature_312_residues
MNGDIAVFAGSSNKQIAEEICTHLNIQPGKINLKKFSDGEISVKVEDNVRGREVFIVQSTSAPANDHLMELILIMDAFRR
ASVSSISVVIPYYGYGRQDRKVEPRVPISARIVADLLEVVGLDRILTMDLHADQIQGFFRVPVDNLHFAPVLAEYVNTKN
INDLVIVSPDSGGAERARAFGKKVNGSLAIIDKRRPKANVSEVMNVIGEIEGKNCILLDDMIDTAGTICKAADALLKHGA
KSVYCAATHGVLSGESVDRINATNFTEVVLANTIAIPESKKIHKLKSLSVAPLFANAIKRIHTNQSVSTLFD

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family [H]

Homologues:

Organism=Homo sapiens, GI4506129, Length=313, Percent_Identity=43.7699680511182, Blast_Score=276, Evalue=2e-74,
Organism=Homo sapiens, GI4506127, Length=313, Percent_Identity=44.0894568690096, Blast_Score=274, Evalue=7e-74,
Organism=Homo sapiens, GI84875539, Length=316, Percent_Identity=43.6708860759494, Blast_Score=272, Evalue=3e-73,
Organism=Homo sapiens, GI28557709, Length=311, Percent_Identity=43.4083601286174, Blast_Score=271, Evalue=6e-73,
Organism=Homo sapiens, GI4506133, Length=348, Percent_Identity=31.0344827586207, Blast_Score=168, Evalue=6e-42,
Organism=Homo sapiens, GI194018537, Length=342, Percent_Identity=31.5789473684211, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI310128524, Length=144, Percent_Identity=31.9444444444444, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI310115209, Length=144, Percent_Identity=31.9444444444444, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI310118259, Length=144, Percent_Identity=31.9444444444444, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI310119946, Length=144, Percent_Identity=31.9444444444444, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI1787458, Length=312, Percent_Identity=49.3589743589744, Blast_Score=325, Evalue=3e-90,
Organism=Caenorhabditis elegans, GI25149168, Length=311, Percent_Identity=45.3376205787781, Blast_Score=285, Evalue=3e-77,
Organism=Caenorhabditis elegans, GI17554702, Length=311, Percent_Identity=45.3376205787781, Blast_Score=283, Evalue=6e-77,
Organism=Caenorhabditis elegans, GI17554704, Length=308, Percent_Identity=45.4545454545455, Blast_Score=283, Evalue=8e-77,
Organism=Caenorhabditis elegans, GI71989924, Length=311, Percent_Identity=45.3376205787781, Blast_Score=283, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI17570245, Length=338, Percent_Identity=30.4733727810651, Blast_Score=174, Evalue=6e-44,
Organism=Saccharomyces cerevisiae, GI6320946, Length=310, Percent_Identity=40.6451612903226, Blast_Score=256, Evalue=3e-69,
Organism=Saccharomyces cerevisiae, GI6319403, Length=311, Percent_Identity=39.871382636656, Blast_Score=252, Evalue=4e-68,
Organism=Saccharomyces cerevisiae, GI6321776, Length=311, Percent_Identity=40.8360128617363, Blast_Score=246, Evalue=4e-66,
Organism=Saccharomyces cerevisiae, GI6322667, Length=195, Percent_Identity=39.4871794871795, Blast_Score=155, Evalue=8e-39,
Organism=Saccharomyces cerevisiae, GI6324511, Length=108, Percent_Identity=37.037037037037, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21355239, Length=313, Percent_Identity=44.7284345047923, Blast_Score=269, Evalue=1e-72,
Organism=Drosophila melanogaster, GI45551540, Length=336, Percent_Identity=41.6666666666667, Blast_Score=257, Evalue=9e-69,
Organism=Drosophila melanogaster, GI24651458, Length=354, Percent_Identity=29.9435028248588, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24651456, Length=354, Percent_Identity=29.9435028248588, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI281362873, Length=354, Percent_Identity=29.9435028248588, Blast_Score=179, Evalue=3e-45,
Organism=Drosophila melanogaster, GI24651454, Length=354, Percent_Identity=29.9435028248588, Blast_Score=179, Evalue=3e-45,
Organism=Drosophila melanogaster, GI24651462, Length=202, Percent_Identity=33.1683168316832, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24651464, Length=202, Percent_Identity=33.1683168316832, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI45552010, Length=202, Percent_Identity=33.1683168316832, Blast_Score=124, Evalue=6e-29,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.7.6.1 [H]

Molecular weight: Translated: 33929; Mature: 33929

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00114 PRPP_SYNTHETASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGDIAVFAGSSNKQIAEEICTHLNIQPGKINLKKFSDGEISVKVEDNVRGREVFIVQST
CCCCEEEEECCCCHHHHHHHHHHCCCCCCEEEEEEECCCEEEEEECCCCCCEEEEEEECC
SAPANDHLMELILIMDAFRRASVSSISVVIPYYGYGRQDRKVEPRVPISARIVADLLEVV
CCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHH
GLDRILTMDLHADQIQGFFRVPVDNLHFAPVLAEYVNTKNINDLVIVSPDSGGAERARAF
CCCCEEEEECCHHHHCCEEECCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHH
GKKVNGSLAIIDKRRPKANVSEVMNVIGEIEGKNCILLDDMIDTAGTICKAADALLKHGA
HHHCCCCEEEEECCCCCCCHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCC
KSVYCAATHGVLSGESVDRINATNFTEVVLANTIAIPESKKIHKLKSLSVAPLFANAIKR
CEEEEEEECCCCCCCCCCCCCCCCHHHEEEEHEEECCCCHHHHHHHCCCHHHHHHHHHHH
IHTNQSVSTLFD
HHCCCCHHHHCC
>Mature Secondary Structure
MNGDIAVFAGSSNKQIAEEICTHLNIQPGKINLKKFSDGEISVKVEDNVRGREVFIVQST
CCCCEEEEECCCCHHHHHHHHHHCCCCCCEEEEEEECCCEEEEEECCCCCCEEEEEEECC
SAPANDHLMELILIMDAFRRASVSSISVVIPYYGYGRQDRKVEPRVPISARIVADLLEVV
CCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHH
GLDRILTMDLHADQIQGFFRVPVDNLHFAPVLAEYVNTKNINDLVIVSPDSGGAERARAF
CCCCEEEEECCHHHHCCEEECCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHH
GKKVNGSLAIIDKRRPKANVSEVMNVIGEIEGKNCILLDDMIDTAGTICKAADALLKHGA
HHHCCCCEEEEECCCCCCCHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCC
KSVYCAATHGVLSGESVDRINATNFTEVVLANTIAIPESKKIHKLKSLSVAPLFANAIKR
CEEEEEEECCCCCCCCCCCCCCCCHHHEEEEHEEECCCCHHHHHHHCCCHHHHHHHHHHH
IHTNQSVSTLFD
HHCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA