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The map label for this gene is gatB [H]

Identifier: 116327112

GI number: 116327112

Start: 338197

End: 339657

Strand: Direct

Name: gatB [H]

Synonym: LBL_0287

Alternate gene names: 116327112

Gene position: 338197-339657 (Clockwise)

Preceding gene: 116327107

Following gene: 116327113

Centisome position: 9.36

GC content: 42.71

Gene sequence:

>1461_bases
TTGGCGGAATTTGAAACGATCATCGGTTTGGAAGTTCATGCACAGCTCAATACGGAATCGAAAATATTCTCTACAAGTGC
GACCAAGTTCGGTTCTCCTCCGAACTCGCAAACTAATCCGGTTTGTTTGGGATTGCCGGGCGCACTTCCGGTTCTCAACG
AGTCCGCTTTGGAAAAAGCGATCATGGCGGGGATTGCGTTCGGGTGTGATATCTCTTTATTTACTAAGTTTGATCGTAAG
AATTATTTTTATCCGGATCTTCCGAAAGGTTATCAAATTTCTCAATTCGATAAACCTATCTGTACTGGAGGTGGAGTCAC
TTTTACGATTAAAGGGGAAGAATCTTCTCGTTATGTGAGACTAACTCGGATTCACATGGAAGAAGATGCGGGAAAACTGA
TACATTCTGCGGATCCGAATATTCCTCAGTCGTATGTCGATTTAAATAGAGCAGGAACACCCCTCATCGAAATTGTTTCG
GAACCGGACATGCGTTCTTCGGATGAGGCGTATTATTATCTTAATTCTTTAAAGTCGATTTTAAAATATATTCGCGTTTC
GGATTGTAACATGGAGGAAGGTTCCCTTCGTTGCGATGCAAATGTTTCGATTCGGCCGAAAGGTTCGGATAAATTCGGAA
CCAGAGTCGAAATTAAGAATCTAAATTCTTTCAAAGCGGTCAAAGCGGCAATCGACTACGAAGTCGAATGGCAAACCGAA
ATGGCTCTCGAAGGTAAGACCTTCCAACAACAAACCAAACTTTGGGATTCCGTAGCGAACAAAACCGTTACGATGAGAAC
CAAAGAGATGAGTCATGATTATCGTTATTTTCCCGACCCGGATCTTCCGGTAATCATTCTTCAAAAGGAAACGGTAGAAT
CTGTTCGTTCCAAATTGCCCGAACTTCCGAACGAAAGAAAAAATCGTTTTGTGGAAAAGTTAGGGCTTCCAAAATACGAT
GCTGAAGTTCTGACCGCCGAAAGGGAAATTGCGGATTACTTTGAAGACGCTTTGAAAGTTTCCGGGGATGCAAAAAAGAC
ATCCAACTGGGTTAAAGACGAAGTTCTTGGTGTAGTAAATAAAGAAAGTATTACGATTTCTGAATTTTCTGTTTCTGCCC
AAAGAATCGGCGGACTTGTAAAATTGATCGCGGACGGAAAAATTTCCGGTAAGATCGCGAAGACCGTATTCGAAGAACTT
CTGATCAGCGACAAAGACGCGGAAACGATCGTAACGGAAAAGAATCTGATCGTGGTTCGTGACGACAAGGAAATCGAAAG
GATCGTAAACGAGGCGATTGCTAACAATCAAGACGCAGTGGCCAAGTACAAAAGTGGTAAAGACCGTGCATTAGGCGCCA
TTGTAGGTTATGTGATGAAAGTTTCCAAAGGAAAAGCGGATCCTGAATTAGTGAACCAAATGCTTCTGGATAAACTGGGA
TCTCTTCCGCCAAAAGAGTGA

Upstream 100 bases:

>100_bases
GATTCCGCTTTCTCGAAACTACATTTAGGGTTGTTGTCCGGAGATTAAGGATAAGAATCCTCAATCGGTTTTGGATGACA
ATCCGGAAAAGGAGATACAA

Downstream 100 bases:

>100_bases
ATGGAATACTCCGTAAACTATCGAGAATTATATAAGAAAGGTGTTTAAAATTTTGTGTATAGATTTGAGGGTTTATCTCA
AAATTCAGAATATTTTATAT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MAEFETIIGLEVHAQLNTESKIFSTSATKFGSPPNSQTNPVCLGLPGALPVLNESALEKAIMAGIAFGCDISLFTKFDRK
NYFYPDLPKGYQISQFDKPICTGGGVTFTIKGEESSRYVRLTRIHMEEDAGKLIHSADPNIPQSYVDLNRAGTPLIEIVS
EPDMRSSDEAYYYLNSLKSILKYIRVSDCNMEEGSLRCDANVSIRPKGSDKFGTRVEIKNLNSFKAVKAAIDYEVEWQTE
MALEGKTFQQQTKLWDSVANKTVTMRTKEMSHDYRYFPDPDLPVIILQKETVESVRSKLPELPNERKNRFVEKLGLPKYD
AEVLTAEREIADYFEDALKVSGDAKKTSNWVKDEVLGVVNKESITISEFSVSAQRIGGLVKLIADGKISGKIAKTVFEEL
LISDKDAETIVTEKNLIVVRDDKEIERIVNEAIANNQDAVAKYKSGKDRALGAIVGYVMKVSKGKADPELVNQMLLDKLG
SLPPKE

Sequences:

>Translated_486_residues
MAEFETIIGLEVHAQLNTESKIFSTSATKFGSPPNSQTNPVCLGLPGALPVLNESALEKAIMAGIAFGCDISLFTKFDRK
NYFYPDLPKGYQISQFDKPICTGGGVTFTIKGEESSRYVRLTRIHMEEDAGKLIHSADPNIPQSYVDLNRAGTPLIEIVS
EPDMRSSDEAYYYLNSLKSILKYIRVSDCNMEEGSLRCDANVSIRPKGSDKFGTRVEIKNLNSFKAVKAAIDYEVEWQTE
MALEGKTFQQQTKLWDSVANKTVTMRTKEMSHDYRYFPDPDLPVIILQKETVESVRSKLPELPNERKNRFVEKLGLPKYD
AEVLTAEREIADYFEDALKVSGDAKKTSNWVKDEVLGVVNKESITISEFSVSAQRIGGLVKLIADGKISGKIAKTVFEEL
LISDKDAETIVTEKNLIVVRDDKEIERIVNEAIANNQDAVAKYKSGKDRALGAIVGYVMKVSKGKADPELVNQMLLDKLG
SLPPKE
>Mature_485_residues
AEFETIIGLEVHAQLNTESKIFSTSATKFGSPPNSQTNPVCLGLPGALPVLNESALEKAIMAGIAFGCDISLFTKFDRKN
YFYPDLPKGYQISQFDKPICTGGGVTFTIKGEESSRYVRLTRIHMEEDAGKLIHSADPNIPQSYVDLNRAGTPLIEIVSE
PDMRSSDEAYYYLNSLKSILKYIRVSDCNMEEGSLRCDANVSIRPKGSDKFGTRVEIKNLNSFKAVKAAIDYEVEWQTEM
ALEGKTFQQQTKLWDSVANKTVTMRTKEMSHDYRYFPDPDLPVIILQKETVESVRSKLPELPNERKNRFVEKLGLPKYDA
EVLTAEREIADYFEDALKVSGDAKKTSNWVKDEVLGVVNKESITISEFSVSAQRIGGLVKLIADGKISGKIAKTVFEELL
ISDKDAETIVTEKNLIVVRDDKEIERIVNEAIANNQDAVAKYKSGKDRALGAIVGYVMKVSKGKADPELVNQMLLDKLGS
LPPKE

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=497, Percent_Identity=35.010060362173, Blast_Score=310, Evalue=2e-84,
Organism=Caenorhabditis elegans, GI115534094, Length=478, Percent_Identity=29.9163179916318, Blast_Score=206, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6319391, Length=506, Percent_Identity=29.8418972332016, Blast_Score=224, Evalue=3e-59,
Organism=Drosophila melanogaster, GI45550808, Length=490, Percent_Identity=35.1020408163265, Blast_Score=298, Evalue=4e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR023168
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 54137; Mature: 54006

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEFETIIGLEVHAQLNTESKIFSTSATKFGSPPNSQTNPVCLGLPGALPVLNESALEKA
CCCCCEEEEEEEEEEECCCCCEEECCCHHCCCCCCCCCCCEEEECCCCCCCCCHHHHHHH
IMAGIAFGCDISLFTKFDRKNYFYPDLPKGYQISQFDKPICTGGGVTFTIKGEESSRYVR
HHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEECCCCEEEEEECCCCCCEEE
LTRIHMEEDAGKLIHSADPNIPQSYVDLNRAGTPLIEIVSEPDMRSSDEAYYYLNSLKSI
EEEEEEHHHHHHHHHCCCCCCCHHHHHHCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHH
LKYIRVSDCNMEEGSLRCDANVSIRPKGSDKFGTRVEIKNLNSFKAVKAAIDYEVEWQTE
HHHHHHCCCCCCCCCEEECCCEEECCCCCCCCCCEEEEECCCHHHHHHHHHCEEEEECEE
MALEGKTFQQQTKLWDSVANKTVTMRTKEMSHDYRYFPDPDLPVIILQKETVESVRSKLP
EEECCCHHHHHHHHHHHHHCCEEEEEEHHCCCCCCCCCCCCCCEEEECHHHHHHHHHHCC
ELPNERKNRFVEKLGLPKYDAEVLTAEREIADYFEDALKVSGDAKKTSNWVKDEVLGVVN
CCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC
KESITISEFSVSAQRIGGLVKLIADGKISGKIAKTVFEELLISDKDAETIVTEKNLIVVR
CCEEEEEHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCEEEECCCEEEEE
DDKEIERIVNEAIANNQDAVAKYKSGKDRALGAIVGYVMKVSKGKADPELVNQMLLDKLG
CCHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHC
SLPPKE
CCCCCC
>Mature Secondary Structure 
AEFETIIGLEVHAQLNTESKIFSTSATKFGSPPNSQTNPVCLGLPGALPVLNESALEKA
CCCCEEEEEEEEEEECCCCCEEECCCHHCCCCCCCCCCCEEEECCCCCCCCCHHHHHHH
IMAGIAFGCDISLFTKFDRKNYFYPDLPKGYQISQFDKPICTGGGVTFTIKGEESSRYVR
HHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEECCCCEEEEEECCCCCCEEE
LTRIHMEEDAGKLIHSADPNIPQSYVDLNRAGTPLIEIVSEPDMRSSDEAYYYLNSLKSI
EEEEEEHHHHHHHHHCCCCCCCHHHHHHCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHH
LKYIRVSDCNMEEGSLRCDANVSIRPKGSDKFGTRVEIKNLNSFKAVKAAIDYEVEWQTE
HHHHHHCCCCCCCCCEEECCCEEECCCCCCCCCCEEEEECCCHHHHHHHHHCEEEEECEE
MALEGKTFQQQTKLWDSVANKTVTMRTKEMSHDYRYFPDPDLPVIILQKETVESVRSKLP
EEECCCHHHHHHHHHHHHHCCEEEEEEHHCCCCCCCCCCCCCCEEEECHHHHHHHHHHCC
ELPNERKNRFVEKLGLPKYDAEVLTAEREIADYFEDALKVSGDAKKTSNWVKDEVLGVVN
CCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC
KESITISEFSVSAQRIGGLVKLIADGKISGKIAKTVFEELLISDKDAETIVTEKNLIVVR
CCEEEEEHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCEEEECCCEEEEE
DDKEIERIVNEAIANNQDAVAKYKSGKDRALGAIVGYVMKVSKGKADPELVNQMLLDKLG
CCHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHC
SLPPKE
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA