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The map label for this gene is pckA [H]

Identifier: 116327107

GI number: 116327107

Start: 333041

End: 334636

Strand: Direct

Name: pckA [H]

Synonym: LBL_0282

Alternate gene names: 116327107

Gene position: 333041-334636 (Clockwise)

Preceding gene: 116327106

Following gene: 116327112

Centisome position: 9.21

GC content: 40.1

Gene sequence:

>1596_bases
ATGCAGGCCCAGACGACTCAGGTAAAAGGCTTGAAGGAACTCGGACTCGAACCTTCTGAAATTTTTCATAATCTTTCCTA
TGATGAAATTTATGAGCATGAGAAAAGAAACGGGGAGACCGTAGTTTCCAGCAATGGAACTATGATGGTAGATACCGGAA
TATTTACCGGTAGATCCCCAAAAGATAAATATTTCGTGGATGAGCCTTCCTCCAATGGAAACATTTGGTGGAGTCATATC
AATTTCAAAGTTTCCGAAGCAATCTTTGACGAGTTATATAAGAAATGTGTAAATTATCTGAATCATAAAAAATTATACGT
TTTTGATGGTTACGCCGGTGCAAATCCGGAAACCAGAGTAAGTCTGCGTGTCGTTTCTGAAAAGGCTTGGCAACACCACT
TTTGTACAAATATGTTTCTTCGTCCTACAAAGGAAGAATTGTCTGGACTGAATCCTGAGTTTACGATCATCAACGCATGC
GGCATTAAGAATGAAAACTTCAAACAACACGGGATGAATTCCGAAGTGTTCGTAATTTTTCATCTTGCTAAGAAAATTTG
TATTATTGGTGGAACCGAATACGGTGGAGAAATGAAAAAAGGTATTTTCTCCGTTATGAATTATAAACTTCCGTTGGAGG
GAATTCTTAGCATGCACTGTTCCGCGAATGTCGGTAAAGAGGGAGATACAGCTCTTTTCTTTGGACTTTCCGGAACCGGT
AAGACAACTCTTTCTACAGACCCGAACCGTAAATTGATCGGAGACGATGAGCATGGTTGGGACGACAATGGGATTTTCAA
CATCGAAGGAGGGTGTTATGCTAAAGTAATTAATTTAGATCCTAAAACCGAACCGGATATCTACGAAGCGATTCGTAGAG
ATGCTCTTCTGGAGAATGTGGTCTATGATCCTCAAACGAAAGTGGTGGATTATTCTTCCGCCGCAAAAACAGAAAATACT
CGTGTTTCTTATCCGATTTTTCACATCAATAATATTCAAGTTCCTTCCAAAGGAGGACATCCAAAAACCATTATATTCTT
AACTTATGATGCGTTTGGAGTTCTTCCGCCTGTTTCCAAATTAAGCATTGAACAGGCGATGTATCACTTCCTTTCAGGAT
ATACTGCAAAAGTTGCGGGAACTGAAAGAGGAATTAAAGAACCTACTGCGACGTTCTCTGCTTGTTTTGGGGCTGCGTTT
ATGACTCTTCACCCGACCAAATATGCTAAGTTACTCGGTGAAAAAATGAAAAAACATAATGTAAGAGCATATTTGATGAA
CACCGGTCTTGTAGGCGGATCTTACGGGGTTGGAAAGCGTATGAATCTTCCTTCTACTCGTAAAATCATAGACGAAATTT
TAAATGGAAATATTGAGAAGTCCGAATTCGTGACTCACCCGGTTTTTCAAGTAGCTTATCCTAAGACGATCAGCGGAATC
GATAGCGCGATACTGGATCCGAGAGAAGCTTGGGCCGACAAAGCCCTTTACGATGCGACCGCTAAGAAATTAGGGGAAAT
GTTCATTAAAAACTTTAAGCAGTACGCAGAAGGTTCTAAAGATTTCGATTTCACTGCTTTTGGTCCAAAAATCTAA

Upstream 100 bases:

>100_bases
AGCTTCTGAAAAGGGAAACCGCCAACCCCATTCTTCTTGAATCGATTGACTTTCGGCTTTAGCATAAAATAATTGAAATA
GAATCCTAAGGAGCAATCAA

Downstream 100 bases:

>100_bases
AACCATTTAGATGCAGGAGATCTTAAGGGATCTCCTGTTTATCTTTTTTATAACCCTTTATCTTTAAGAGTGTAGAAATT
TCTGTATTTGTTTACACAAT

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 531; Mature: 531

Protein sequence:

>531_residues
MQAQTTQVKGLKELGLEPSEIFHNLSYDEIYEHEKRNGETVVSSNGTMMVDTGIFTGRSPKDKYFVDEPSSNGNIWWSHI
NFKVSEAIFDELYKKCVNYLNHKKLYVFDGYAGANPETRVSLRVVSEKAWQHHFCTNMFLRPTKEELSGLNPEFTIINAC
GIKNENFKQHGMNSEVFVIFHLAKKICIIGGTEYGGEMKKGIFSVMNYKLPLEGILSMHCSANVGKEGDTALFFGLSGTG
KTTLSTDPNRKLIGDDEHGWDDNGIFNIEGGCYAKVINLDPKTEPDIYEAIRRDALLENVVYDPQTKVVDYSSAAKTENT
RVSYPIFHINNIQVPSKGGHPKTIIFLTYDAFGVLPPVSKLSIEQAMYHFLSGYTAKVAGTERGIKEPTATFSACFGAAF
MTLHPTKYAKLLGEKMKKHNVRAYLMNTGLVGGSYGVGKRMNLPSTRKIIDEILNGNIEKSEFVTHPVFQVAYPKTISGI
DSAILDPREAWADKALYDATAKKLGEMFIKNFKQYAEGSKDFDFTAFGPKI

Sequences:

>Translated_531_residues
MQAQTTQVKGLKELGLEPSEIFHNLSYDEIYEHEKRNGETVVSSNGTMMVDTGIFTGRSPKDKYFVDEPSSNGNIWWSHI
NFKVSEAIFDELYKKCVNYLNHKKLYVFDGYAGANPETRVSLRVVSEKAWQHHFCTNMFLRPTKEELSGLNPEFTIINAC
GIKNENFKQHGMNSEVFVIFHLAKKICIIGGTEYGGEMKKGIFSVMNYKLPLEGILSMHCSANVGKEGDTALFFGLSGTG
KTTLSTDPNRKLIGDDEHGWDDNGIFNIEGGCYAKVINLDPKTEPDIYEAIRRDALLENVVYDPQTKVVDYSSAAKTENT
RVSYPIFHINNIQVPSKGGHPKTIIFLTYDAFGVLPPVSKLSIEQAMYHFLSGYTAKVAGTERGIKEPTATFSACFGAAF
MTLHPTKYAKLLGEKMKKHNVRAYLMNTGLVGGSYGVGKRMNLPSTRKIIDEILNGNIEKSEFVTHPVFQVAYPKTISGI
DSAILDPREAWADKALYDATAKKLGEMFIKNFKQYAEGSKDFDFTAFGPKI
>Mature_531_residues
MQAQTTQVKGLKELGLEPSEIFHNLSYDEIYEHEKRNGETVVSSNGTMMVDTGIFTGRSPKDKYFVDEPSSNGNIWWSHI
NFKVSEAIFDELYKKCVNYLNHKKLYVFDGYAGANPETRVSLRVVSEKAWQHHFCTNMFLRPTKEELSGLNPEFTIINAC
GIKNENFKQHGMNSEVFVIFHLAKKICIIGGTEYGGEMKKGIFSVMNYKLPLEGILSMHCSANVGKEGDTALFFGLSGTG
KTTLSTDPNRKLIGDDEHGWDDNGIFNIEGGCYAKVINLDPKTEPDIYEAIRRDALLENVVYDPQTKVVDYSSAAKTENT
RVSYPIFHINNIQVPSKGGHPKTIIFLTYDAFGVLPPVSKLSIEQAMYHFLSGYTAKVAGTERGIKEPTATFSACFGAAF
MTLHPTKYAKLLGEKMKKHNVRAYLMNTGLVGGSYGVGKRMNLPSTRKIIDEILNGNIEKSEFVTHPVFQVAYPKTISGI
DSAILDPREAWADKALYDATAKKLGEMFIKNFKQYAEGSKDFDFTAFGPKI

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family [H]

Homologues:

Organism=Escherichia coli, GI1789807, Length=524, Percent_Identity=53.2442748091603, Blast_Score=577, Evalue=1e-166,
Organism=Saccharomyces cerevisiae, GI6322950, Length=528, Percent_Identity=46.2121212121212, Blast_Score=473, Evalue=1e-134,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994 [H]

Pfam domain/function: PF01293 PEPCK_ATP [H]

EC number: =4.1.1.49 [H]

Molecular weight: Translated: 59316; Mature: 59316

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAQTTQVKGLKELGLEPSEIFHNLSYDEIYEHEKRNGETVVSSNGTMMVDTGIFTGRSP
CCCCCCHHHHHHHHCCCHHHHHHCCCHHHHHHHHCCCCCEEEECCCCEEEEECEECCCCC
KDKYFVDEPSSNGNIWWSHINFKVSEAIFDELYKKCVNYLNHKKLYVFDGYAGANPETRV
CCCEEEECCCCCCCEEEEEECEEHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEE
SLRVVSEKAWQHHFCTNMFLRPTKEELSGLNPEFTIINACGIKNENFKQHGMNSEVFVIF
EEEEEHHHHHHHHHHHHHCCCCCHHHHCCCCCCEEEEEECCCCCCCHHHCCCCCCEEEEE
HLAKKICIIGGTEYGGEMKKGIFSVMNYKLPLEGILSMHCSANVGKEGDTALFFGLSGTG
EEHHCEEEECCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCEEEEEEECCCC
KTTLSTDPNRKLIGDDEHGWDDNGIFNIEGGCYAKVINLDPKTEPDIYEAIRRDALLENV
CEEECCCCCCCCCCCCCCCCCCCCEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHHH
VYDPQTKVVDYSSAAKTENTRVSYPIFHINNIQVPSKGGHPKTIIFLTYDAFGVLPPVSK
HCCCCCCEEECCCCCCCCCCEEEEEEEEECEEECCCCCCCCCEEEEEEECCCCCCCCHHH
LSIEQAMYHFLSGYTAKVAGTERGIKEPTATFSACFGAAFMTLHPTKYAKLLGEKMKKHN
HHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHEEECCHHHHHHHHHHHHHCC
VRAYLMNTGLVGGSYGVGKRMNLPSTRKIIDEILNGNIEKSEFVTHPVFQVAYPKTISGI
CEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHCCCHHEEECCCHHHCC
DSAILDPREAWADKALYDATAKKLGEMFIKNFKQYAEGSKDFDFTAFGPKI
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCC
>Mature Secondary Structure
MQAQTTQVKGLKELGLEPSEIFHNLSYDEIYEHEKRNGETVVSSNGTMMVDTGIFTGRSP
CCCCCCHHHHHHHHCCCHHHHHHCCCHHHHHHHHCCCCCEEEECCCCEEEEECEECCCCC
KDKYFVDEPSSNGNIWWSHINFKVSEAIFDELYKKCVNYLNHKKLYVFDGYAGANPETRV
CCCEEEECCCCCCCEEEEEECEEHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEE
SLRVVSEKAWQHHFCTNMFLRPTKEELSGLNPEFTIINACGIKNENFKQHGMNSEVFVIF
EEEEEHHHHHHHHHHHHHCCCCCHHHHCCCCCCEEEEEECCCCCCCHHHCCCCCCEEEEE
HLAKKICIIGGTEYGGEMKKGIFSVMNYKLPLEGILSMHCSANVGKEGDTALFFGLSGTG
EEHHCEEEECCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCEEEEEEECCCC
KTTLSTDPNRKLIGDDEHGWDDNGIFNIEGGCYAKVINLDPKTEPDIYEAIRRDALLENV
CEEECCCCCCCCCCCCCCCCCCCCEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHHH
VYDPQTKVVDYSSAAKTENTRVSYPIFHINNIQVPSKGGHPKTIIFLTYDAFGVLPPVSK
HCCCCCCEEECCCCCCCCCCEEEEEEEEECEEECCCCCCCCCEEEEEEECCCCCCCCHHH
LSIEQAMYHFLSGYTAKVAGTERGIKEPTATFSACFGAAFMTLHPTKYAKLLGEKMKKHN
HHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHEEECCHHHHHHHHHHHHHCC
VRAYLMNTGLVGGSYGVGKRMNLPSTRKIIDEILNGNIEKSEFVTHPVFQVAYPKTISGI
CEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHCCCHHEEECCCHHHCC
DSAILDPREAWADKALYDATAKKLGEMFIKNFKQYAEGSKDFDFTAFGPKI
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA