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The map label for this gene is gyrA [H]

Identifier: 116327081

GI number: 116327081

Start: 304447

End: 305835

Strand: Reverse

Name: gyrA [H]

Synonym: LBL_0254

Alternate gene names: 116327081

Gene position: 305835-304447 (Counterclockwise)

Preceding gene: 116327090

Following gene: 116327080

Centisome position: 8.46

GC content: 40.96

Gene sequence:

>1389_bases
ATGAAGAATTCCAATCCACCAAAATTAAAGGACTCGTTTCCCAAACGCCCTTTTGAAGATCAAGTTAATGACGATCAGAG
AAAATATTCGCGTTATGTATGCGATTCTCGGGCGATTCCACACGAAATCGACGGTCTAAAACCCGTTCAAAGAAGAATTC
TTTGGGCGATGTGGAACTCGGACGCAAGAAACCGTTATACGAAAACCGTAAAAGTTGCCGGTCTTGCAATGGGGTATCAT
CCTCACGGAGATAAATCAATCCAAGACGCACTTTCACAAATGGCCCAGGACTTTACCTTCGCCAATAATATTCCGCTCGT
TTCGGGAGAAGGAACTTTCGGTGACGTTTTGGATCCGAGCGCGATTGCCTCCCCTCGTTATACGGAAGTGAAATTATCCG
ACTTTGTAAAAGATTTGGGATTCTTTGAAAGTTTGCCGGACATCGATTATGTTAAGAACTATGACGAGACGGAAGACGAA
CCGATTCATTTTGTAGGAAAAGTTCCGATCGTTCTTTTGAACAACATCCAAGGGATTGCGACCGGATTTCGTTGTTTTAT
TCCGGGTCATAGACTTGCGGACATCGTCAAGTCTCAGATCAATTATCTCAAAACCAAAAAACCTCTTTCTTTAAAACCTT
GGTACAAAGACTTCAACGGTGAAGTGAAAATGGCCGAAACCGAAACAGGTAATATCACTATGTCCACGACGTTCGCATTC
AAGTGGGAAGGTGACATTCTTTATCTCACCGATTCTCCGATGAATTGGAACCGAGAGAAGGTCATCTCTTTGCTCGATGA
TATTTTGGAGAGAAAAGATTCTTGGTTGAAGGAATACGTGGATTATTCCAGCCAGACTTTTAGAATCGAACTTCAATATA
AGAAGGGGGAGAAGCCAAGCCAAAAGGAAATCATGGCTCTCTTCAATAAGGAAGACATTCAAACTCTTGCGATGAACGTC
ATTACGTATGACGGTAAACTAAAAAACTTTAAACCCGAAGAAATTATCAAACGTTTCTGCGACTTCCGCAAAACCCACCT
TATCCGTAGATTCAAACGTTTATCCGGTCTGGAAGAAGAAAAAATCGAAAGAAACTCGGAATTGATTCGTTTTATTAAAG
AGAAATGGAACGAGAAAGTAATCGGAATCAAATCCAAAAAGGACTTTGAAGACAAACTTAAGGCTTCTAAATTCAAGTAT
TTCGAGTGGTTGTCCACGATTCCGGTCTACAGAATGACCATTGAAGAAGTTAAAAAATGCGAAGAAGCGATCATAGAAGC
AAAAACGACCCTGGCTCGTTATCAAGGTCTGGTCAAAGAGGATAAAAAACTGACCGACTATATGATCACCGAGTTGAACG
AACTTCAAAACAAATGGGATAAAGTATGA

Upstream 100 bases:

>100_bases
GAATTGTTCAAATATTCGCTTCGAAAATACTGTAATTAAATCCGGTCATTTTTTTGCAGTTGACAGGATAGTCTTCTAGA
ATTTCGTTGGCCAAAACCGT

Downstream 100 bases:

>100_bases
GCGCCGATAAAACCAAAGTAAAAGAAAAATCATCCCAAGAAAGAAATTTTAAAAAGTTATCCAACGTGGAACACGTCCGA
ATGAGAACCGGTATGTGGTT

Product: DNA topoisomerase (ATP-hydrolyzing), subunit A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MKNSNPPKLKDSFPKRPFEDQVNDDQRKYSRYVCDSRAIPHEIDGLKPVQRRILWAMWNSDARNRYTKTVKVAGLAMGYH
PHGDKSIQDALSQMAQDFTFANNIPLVSGEGTFGDVLDPSAIASPRYTEVKLSDFVKDLGFFESLPDIDYVKNYDETEDE
PIHFVGKVPIVLLNNIQGIATGFRCFIPGHRLADIVKSQINYLKTKKPLSLKPWYKDFNGEVKMAETETGNITMSTTFAF
KWEGDILYLTDSPMNWNREKVISLLDDILERKDSWLKEYVDYSSQTFRIELQYKKGEKPSQKEIMALFNKEDIQTLAMNV
ITYDGKLKNFKPEEIIKRFCDFRKTHLIRRFKRLSGLEEEKIERNSELIRFIKEKWNEKVIGIKSKKDFEDKLKASKFKY
FEWLSTIPVYRMTIEEVKKCEEAIIEAKTTLARYQGLVKEDKKLTDYMITELNELQNKWDKV

Sequences:

>Translated_462_residues
MKNSNPPKLKDSFPKRPFEDQVNDDQRKYSRYVCDSRAIPHEIDGLKPVQRRILWAMWNSDARNRYTKTVKVAGLAMGYH
PHGDKSIQDALSQMAQDFTFANNIPLVSGEGTFGDVLDPSAIASPRYTEVKLSDFVKDLGFFESLPDIDYVKNYDETEDE
PIHFVGKVPIVLLNNIQGIATGFRCFIPGHRLADIVKSQINYLKTKKPLSLKPWYKDFNGEVKMAETETGNITMSTTFAF
KWEGDILYLTDSPMNWNREKVISLLDDILERKDSWLKEYVDYSSQTFRIELQYKKGEKPSQKEIMALFNKEDIQTLAMNV
ITYDGKLKNFKPEEIIKRFCDFRKTHLIRRFKRLSGLEEEKIERNSELIRFIKEKWNEKVIGIKSKKDFEDKLKASKFKY
FEWLSTIPVYRMTIEEVKKCEEAIIEAKTTLARYQGLVKEDKKLTDYMITELNELQNKWDKV
>Mature_462_residues
MKNSNPPKLKDSFPKRPFEDQVNDDQRKYSRYVCDSRAIPHEIDGLKPVQRRILWAMWNSDARNRYTKTVKVAGLAMGYH
PHGDKSIQDALSQMAQDFTFANNIPLVSGEGTFGDVLDPSAIASPRYTEVKLSDFVKDLGFFESLPDIDYVKNYDETEDE
PIHFVGKVPIVLLNNIQGIATGFRCFIPGHRLADIVKSQINYLKTKKPLSLKPWYKDFNGEVKMAETETGNITMSTTFAF
KWEGDILYLTDSPMNWNREKVISLLDDILERKDSWLKEYVDYSSQTFRIELQYKKGEKPSQKEIMALFNKEDIQTLAMNV
ITYDGKLKNFKPEEIIKRFCDFRKTHLIRRFKRLSGLEEEKIERNSELIRFIKEKWNEKVIGIKSKKDFEDKLKASKFKY
FEWLSTIPVYRMTIEEVKKCEEAIIEAKTTLARYQGLVKEDKKLTDYMITELNELQNKWDKV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0188

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the topoisomerase gyrA/parC subunit family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=370, Percent_Identity=27.8378378378378, Blast_Score=113, Evalue=4e-25,
Organism=Homo sapiens, GI19913406, Length=361, Percent_Identity=27.7008310249307, Blast_Score=107, Evalue=3e-23,
Organism=Escherichia coli, GI1789396, Length=473, Percent_Identity=26.4270613107822, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1788562, Length=358, Percent_Identity=26.8156424581006, Blast_Score=123, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17535065, Length=379, Percent_Identity=28.4960422163588, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI212645657, Length=371, Percent_Identity=28.0323450134771, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI212645845, Length=434, Percent_Identity=27.1889400921659, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI115534553, Length=311, Percent_Identity=27.6527331189711, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324241, Length=373, Percent_Identity=24.1286863270777, Blast_Score=91, Evalue=5e-19,
Organism=Drosophila melanogaster, GI17136538, Length=378, Percent_Identity=27.2486772486773, Blast_Score=119, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020899
- InterPro:   IPR005743
- InterPro:   IPR006691
- InterPro:   IPR002205
- InterPro:   IPR013758
- InterPro:   IPR013757
- InterPro:   IPR013760 [H]

Pfam domain/function: PF03989 DNA_gyraseA_C; PF00521 DNA_topoisoIV [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 54103; Mature: 54103

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNSNPPKLKDSFPKRPFEDQVNDDQRKYSRYVCDSRAIPHEIDGLKPVQRRILWAMWNS
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCC
DARNRYTKTVKVAGLAMGYHPHGDKSIQDALSQMAQDFTFANNIPLVSGEGTFGDVLDPS
CHHHHHHHHEEEEHEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCH
AIASPRYTEVKLSDFVKDLGFFESLPDIDYVKNYDETEDEPIHFVGKVPIVLLNNIQGIA
HCCCCCCCEEEHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCEEEECCCCEEEECCCHHHH
TGFRCFIPGHRLADIVKSQINYLKTKKPLSLKPWYKDFNGEVKMAETETGNITMSTTFAF
CCHHEECCCHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCEEEEECCCCCEEEEEEEEE
KWEGDILYLTDSPMNWNREKVISLLDDILERKDSWLKEYVDYSSQTFRIELQYKKGEKPS
EECCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCC
QKEIMALFNKEDIQTLAMNVITYDGKLKNFKPEEIIKRFCDFRKTHLIRRFKRLSGLEEE
HHHHHHHHCHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
KIERNSELIRFIKEKWNEKVIGIKSKKDFEDKLKASKFKYFEWLSTIPVYRMTIEEVKKC
HHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
EEAIIEAKTTLARYQGLVKEDKKLTDYMITELNELQNKWDKV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKNSNPPKLKDSFPKRPFEDQVNDDQRKYSRYVCDSRAIPHEIDGLKPVQRRILWAMWNS
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCC
DARNRYTKTVKVAGLAMGYHPHGDKSIQDALSQMAQDFTFANNIPLVSGEGTFGDVLDPS
CHHHHHHHHEEEEHEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCH
AIASPRYTEVKLSDFVKDLGFFESLPDIDYVKNYDETEDEPIHFVGKVPIVLLNNIQGIA
HCCCCCCCEEEHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCEEEECCCCEEEECCCHHHH
TGFRCFIPGHRLADIVKSQINYLKTKKPLSLKPWYKDFNGEVKMAETETGNITMSTTFAF
CCHHEECCCHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCEEEEECCCCCEEEEEEEEE
KWEGDILYLTDSPMNWNREKVISLLDDILERKDSWLKEYVDYSSQTFRIELQYKKGEKPS
EECCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCC
QKEIMALFNKEDIQTLAMNVITYDGKLKNFKPEEIIKRFCDFRKTHLIRRFKRLSGLEEE
HHHHHHHHCHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
KIERNSELIRFIKEKWNEKVIGIKSKKDFEDKLKASKFKYFEWLSTIPVYRMTIEEVKKC
HHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
EEAIIEAKTTLARYQGLVKEDKKLTDYMITELNELQNKWDKV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8384814; 10688204 [H]