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The map label for this gene is cysN [H]

Identifier: 116327058

GI number: 116327058

Start: 277452

End: 278702

Strand: Direct

Name: cysN [H]

Synonym: LBL_0231

Alternate gene names: 116327058

Gene position: 277452-278702 (Clockwise)

Preceding gene: 116327057

Following gene: 116327059

Centisome position: 7.68

GC content: 46.04

Gene sequence:

>1251_bases
ATGGATTTATTACGTTTTATCACTGCGGGAAGCGTGGATGACGGAAAATCCACATTGATCGGAAGACTTCTCTATGATAG
CAAATCGATTTTCGAAGACCAACTCGAAGCGATCGAAAAAACCGCGCGTGGAAACAACGGCCAAATCAACTTGGCTCTTC
TTACCGACGGCCTCAAGGCCGAAAGAGAACAGGGAATTACGATTGACGTCGCTTATAAGTATTTTTCAACTCCGAAACGA
AAGTTCATCATCGCCGACGCTCCGGGCCACGTTCAATACACGCGGAACATGGTGACGGGTGCGTCTAACTCCAATTTGGC
GATCATCTTGATCGACGCACGGAAGGGCGTAATCGAACAAACCTATCGCCATTCTTACATCGCTTCGATGCTTCGGATTC
CTCACCTCGTTGTTTGTGTCAACAAGATGGACTTGGTAGAATTCTCCCAAACTCGTTACGAAGAAATTAAAGCGGAATAT
CTGAACTTCGCGTCTCGATTGGACATTAAGGATATTGAATTTATTCCGATCAGCGCTCTCAACGGAGACAACGTGGTCGA
TAAGTCCGAAAATCTTTCCTGGTATGAAGGACGCACTTTGCTCAGTCATCTCGAAGAAGTTTATATCGAAAGCGACGAGA
ACATAGAAGACGCTCGTTTTCCGGTTCAATACGTGATTCGTCCTCTTTCCGACGAACATCACGACTATAGAGGTTACGCG
GGGCAGGTCCGCAGCGGAATTTTCAGAAAGGGTGATTCGATCACAGTTCTTCCTAGCGGGTTTACAAGTACGATCGAGGC
CATCGATACTTACGGCCTGGAAGTGGAAGAGGCGTTTCCTCCTATGTCCGTTACAATCCGTTTAAAAGACGAAATCGATA
TTTCCCGAGGGGACATGATCGTCCAATCCGGCAACCTTCCTATCGTCTCTCAGGATTTAGAAGCTAATATCTGTTGGATG
GATTCAAAAGTTTTATTATCCGGAAATAAATATTTACTTCGCCAGACGACCAATGCTGTAAAGGCAATCGTAAAAGAAAT
CGAATTCAAAATTGCCCCCGATACCCATGAAAAACAGGACGCATCGAAAGGCCTTACGTTAAACGAAATTGGTAAAATCA
AAATCCGGACCGCAAAACCTGTGAGCTTTGACGAATATAGAACCAACCGCTCCACTGGAAGTTTTATCCTCGTGGACGAG
GGAACCAATTCCACCGTCGGCGCGGGAATGATTACCGGAGTCGGAGCCTAA

Upstream 100 bases:

>100_bases
TCGAAATCCAGACGACTCGGACTACCGAAAGAGGTTCTAGGCTCGACGACAAACGTTCCGAAGCGGCTATGGAAGACAGA
AAAAGGGGAGGATACTTCTG

Downstream 100 bases:

>100_bases
TTCGTGTTAAACGCCGATACGGATAAAGATCTTAAACCAGGCAAGGTTTATCTTGTCGGAGCCGGACCGGGAAATCCGGA
AGATTTGACTTTGCGGGCGT

Product: Sulfate adenylyltransferase

Products: NA

Alternate protein names: Sulfate adenylyltransferase subunit 1; ATP-sulfurylase large subunit; Sulfate adenylate transferase; SAT; Adenylyl-sulfate kinase; APS kinase; ATP adenosine-5'-phosphosulfate 3'-phosphotransferase [H]

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MDLLRFITAGSVDDGKSTLIGRLLYDSKSIFEDQLEAIEKTARGNNGQINLALLTDGLKAEREQGITIDVAYKYFSTPKR
KFIIADAPGHVQYTRNMVTGASNSNLAIILIDARKGVIEQTYRHSYIASMLRIPHLVVCVNKMDLVEFSQTRYEEIKAEY
LNFASRLDIKDIEFIPISALNGDNVVDKSENLSWYEGRTLLSHLEEVYIESDENIEDARFPVQYVIRPLSDEHHDYRGYA
GQVRSGIFRKGDSITVLPSGFTSTIEAIDTYGLEVEEAFPPMSVTIRLKDEIDISRGDMIVQSGNLPIVSQDLEANICWM
DSKVLLSGNKYLLRQTTNAVKAIVKEIEFKIAPDTHEKQDASKGLTLNEIGKIKIRTAKPVSFDEYRTNRSTGSFILVDE
GTNSTVGAGMITGVGA

Sequences:

>Translated_416_residues
MDLLRFITAGSVDDGKSTLIGRLLYDSKSIFEDQLEAIEKTARGNNGQINLALLTDGLKAEREQGITIDVAYKYFSTPKR
KFIIADAPGHVQYTRNMVTGASNSNLAIILIDARKGVIEQTYRHSYIASMLRIPHLVVCVNKMDLVEFSQTRYEEIKAEY
LNFASRLDIKDIEFIPISALNGDNVVDKSENLSWYEGRTLLSHLEEVYIESDENIEDARFPVQYVIRPLSDEHHDYRGYA
GQVRSGIFRKGDSITVLPSGFTSTIEAIDTYGLEVEEAFPPMSVTIRLKDEIDISRGDMIVQSGNLPIVSQDLEANICWM
DSKVLLSGNKYLLRQTTNAVKAIVKEIEFKIAPDTHEKQDASKGLTLNEIGKIKIRTAKPVSFDEYRTNRSTGSFILVDE
GTNSTVGAGMITGVGA
>Mature_416_residues
MDLLRFITAGSVDDGKSTLIGRLLYDSKSIFEDQLEAIEKTARGNNGQINLALLTDGLKAEREQGITIDVAYKYFSTPKR
KFIIADAPGHVQYTRNMVTGASNSNLAIILIDARKGVIEQTYRHSYIASMLRIPHLVVCVNKMDLVEFSQTRYEEIKAEY
LNFASRLDIKDIEFIPISALNGDNVVDKSENLSWYEGRTLLSHLEEVYIESDENIEDARFPVQYVIRPLSDEHHDYRGYA
GQVRSGIFRKGDSITVLPSGFTSTIEAIDTYGLEVEEAFPPMSVTIRLKDEIDISRGDMIVQSGNLPIVSQDLEANICWM
DSKVLLSGNKYLLRQTTNAVKAIVKEIEFKIAPDTHEKQDASKGLTLNEIGKIKIRTAKPVSFDEYRTNRSTGSFILVDE
GTNSTVGAGMITGVGA

Specific function: APS kinase catalyzes the synthesis of activated sulfate [H]

COG id: COG2895

COG function: function code P; GTPases - Sulfate adenylate transferase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the APS kinase family [H]

Homologues:

Organism=Homo sapiens, GI4503471, Length=447, Percent_Identity=28.8590604026846, Blast_Score=168, Evalue=7e-42,
Organism=Homo sapiens, GI194018522, Length=422, Percent_Identity=29.1469194312796, Blast_Score=168, Evalue=9e-42,
Organism=Homo sapiens, GI194018520, Length=422, Percent_Identity=29.1469194312796, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI194097354, Length=422, Percent_Identity=29.1469194312796, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI46094014, Length=422, Percent_Identity=28.1990521327014, Blast_Score=162, Evalue=6e-40,
Organism=Homo sapiens, GI223555963, Length=435, Percent_Identity=28.735632183908, Blast_Score=162, Evalue=6e-40,
Organism=Homo sapiens, GI4503475, Length=447, Percent_Identity=28.1879194630872, Blast_Score=162, Evalue=8e-40,
Organism=Homo sapiens, GI5729864, Length=435, Percent_Identity=28.735632183908, Blast_Score=161, Evalue=8e-40,
Organism=Homo sapiens, GI34147630, Length=331, Percent_Identity=27.4924471299094, Blast_Score=82, Evalue=9e-16,
Organism=Escherichia coli, GI1789108, Length=414, Percent_Identity=49.5169082125604, Blast_Score=385, Evalue=1e-108,
Organism=Escherichia coli, GI1789737, Length=138, Percent_Identity=28.9855072463768, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1790412, Length=138, Percent_Identity=28.9855072463768, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI2367247, Length=155, Percent_Identity=30.9677419354839, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17552884, Length=447, Percent_Identity=29.3064876957494, Blast_Score=172, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17569207, Length=447, Percent_Identity=29.3064876957494, Blast_Score=172, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI32566303, Length=440, Percent_Identity=28.4090909090909, Blast_Score=152, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI32566629, Length=424, Percent_Identity=27.3584905660377, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI115532067, Length=274, Percent_Identity=30.6569343065693, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI115532065, Length=274, Percent_Identity=30.6569343065693, Blast_Score=141, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI32566301, Length=142, Percent_Identity=38.0281690140845, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI25141371, Length=277, Percent_Identity=27.4368231046931, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17556456, Length=329, Percent_Identity=26.4437689969605, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6325337, Length=445, Percent_Identity=28.9887640449438, Blast_Score=176, Evalue=7e-45,
Organism=Saccharomyces cerevisiae, GI6319594, Length=445, Percent_Identity=28.9887640449438, Blast_Score=176, Evalue=7e-45,
Organism=Saccharomyces cerevisiae, GI6322937, Length=324, Percent_Identity=32.0987654320988, Blast_Score=150, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6320377, Length=433, Percent_Identity=30.715935334873, Blast_Score=137, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6324761, Length=161, Percent_Identity=34.7826086956522, Blast_Score=84, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24652838, Length=454, Percent_Identity=28.1938325991189, Blast_Score=166, Evalue=4e-41,
Organism=Drosophila melanogaster, GI17137572, Length=454, Percent_Identity=28.1938325991189, Blast_Score=166, Evalue=4e-41,
Organism=Drosophila melanogaster, GI45553807, Length=447, Percent_Identity=27.7404921700224, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI45553816, Length=447, Percent_Identity=27.7404921700224, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24651721, Length=447, Percent_Identity=27.7404921700224, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI17864154, Length=447, Percent_Identity=27.7404921700224, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI17137380, Length=425, Percent_Identity=28.7058823529412, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI45550900, Length=436, Percent_Identity=27.7522935779816, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI281363316, Length=320, Percent_Identity=27.1875, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI17864358, Length=320, Percent_Identity=27.1875, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI19921738, Length=146, Percent_Identity=31.5068493150685, Blast_Score=72, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002891
- InterPro:   IPR000795
- InterPro:   IPR011779
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF01583 APS_kinase; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: =2.7.7.4; =2.7.1.25 [H]

Molecular weight: Translated: 46423; Mature: 46423

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLLRFITAGSVDDGKSTLIGRLLYDSKSIFEDQLEAIEKTARGNNGQINLALLTDGLKA
CCHHHEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHH
EREQGITIDVAYKYFSTPKRKFIIADAPGHVQYTRNMVTGASNSNLAIILIDARKGVIEQ
HHHCCCEEEEEEHHHCCCCEEEEEECCCCCEEEEHHEEECCCCCCEEEEEEECCCCHHHH
TYRHSYIASMLRIPHLVVCVNKMDLVEFSQTRYEEIKAEYLNFASRLDIKDIEFIPISAL
HHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEC
NGDNVVDKSENLSWYEGRTLLSHLEEVYIESDENIEDARFPVQYVIRPLSDEHHDYRGYA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHCCCHHHHHCCCCCCCCCCCCCH
GQVRSGIFRKGDSITVLPSGFTSTIEAIDTYGLEVEEAFPPMSVTIRLKDEIDISRGDMI
HHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCCHHHCCCCEEEEEEEECCCCCCCCCEE
VQSGNLPIVSQDLEANICWMDSKVLLSGNKYLLRQTTNAVKAIVKEIEFKIAPDTHEKQD
EECCCCCEEECCCCCEEEEECCEEEEECCEEEEEHHHHHHHHHHHHHEEEECCCCCCCHH
ASKGLTLNEIGKIKIRTAKPVSFDEYRTNRSTGSFILVDEGTNSTVGAGMITGVGA
HHCCCCHHCCCEEEEEECCCCCHHHHHCCCCCCCEEEEECCCCCCCCCCCEECCCC
>Mature Secondary Structure
MDLLRFITAGSVDDGKSTLIGRLLYDSKSIFEDQLEAIEKTARGNNGQINLALLTDGLKA
CCHHHEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHH
EREQGITIDVAYKYFSTPKRKFIIADAPGHVQYTRNMVTGASNSNLAIILIDARKGVIEQ
HHHCCCEEEEEEHHHCCCCEEEEEECCCCCEEEEHHEEECCCCCCEEEEEEECCCCHHHH
TYRHSYIASMLRIPHLVVCVNKMDLVEFSQTRYEEIKAEYLNFASRLDIKDIEFIPISAL
HHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEC
NGDNVVDKSENLSWYEGRTLLSHLEEVYIESDENIEDARFPVQYVIRPLSDEHHDYRGYA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHCCCHHHHHCCCCCCCCCCCCCH
GQVRSGIFRKGDSITVLPSGFTSTIEAIDTYGLEVEEAFPPMSVTIRLKDEIDISRGDMI
HHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCCHHHCCCCEEEEEEEECCCCCCCCCEE
VQSGNLPIVSQDLEANICWMDSKVLLSGNKYLLRQTTNAVKAIVKEIEFKIAPDTHEKQD
EECCCCCEEECCCCCEEEEECCEEEEECCEEEEEHHHHHHHHHHHHHEEEECCCCCCCHH
ASKGLTLNEIGKIKIRTAKPVSFDEYRTNRSTGSFILVDEGTNSTVGAGMITGVGA
HHCCCCHHCCCEEEEEECCCCCHHHHHCCCCCCCEEEEECCCCCCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]