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The map label for this gene is cysG [H]

Identifier: 116327059

GI number: 116327059

Start: 278706

End: 279539

Strand: Direct

Name: cysG [H]

Synonym: LBL_0232

Alternate gene names: 116327059

Gene position: 278706-279539 (Clockwise)

Preceding gene: 116327058

Following gene: 116327060

Centisome position: 7.71

GC content: 43.76

Gene sequence:

>834_bases
GTGTTAAACGCCGATACGGATAAAGATCTTAAACCAGGCAAGGTTTATCTTGTCGGAGCCGGACCGGGAAATCCGGAAGA
TTTGACTTTGCGGGCGTATCGAATTCTCGCTAAGGCCGAAGTCATTTTATATGACGCACTTTTAGATCCTTCGTTTCTCG
AAGTTTTTCCCAAAGACGCCGTCATTCACTACGTAGGGAAACGTTCCGGCGCACATTCCGCGACTCAGGAAGAGATTAAC
CGGCTTCTTCTTTCCTACGCTCTTCAAGGAAAAAATGTGGTTCGTCTAAAAGGCGGAGATCCTTTCGTCTTCGGCAGAGG
TGGGGAAGAATTAATTACATTAATTAATCATAATATAACTTACGAGATCATTCCCGGAGTCAGTTCTTTGAATGCGGGTT
CGTCTTTTGCCGGTTTCCCGTTGACTCACAGAGGTTTGTCCCGTCAGGTTTTGATCATGGACGGTCATACCGTTCTGAGC
GAAGAGACCGATTGGACTTGGTTCGCGAAGTTTAAAGGAACGATCGCGCTTTTTATGGGAACAGCTTCCCTTCCTAAAAT
CGCAAAACTACTTTTACAACACGGAAGTGATCCCGACTTGCCGGTGGCTCTCGTCGAAAATGCTTCTTTAGAAAATTGTA
ATATTCAAACTTGTGCCCTTAAGGATGCGGCGGATTTTTGTCTTGAAAAAAAGGCAAAAGGTCCTGGAATCATTTATATC
GGAAAAGTAGTCCGATTTCTGGAAAACAAAAGATCGATTTCAACTTTCTCTCAAGATTTAGGAGGAACCCGGTTTGATCA
GGTTTTCTCCGAAATTCAAGGAAAAAAAGAATGA

Upstream 100 bases:

>100_bases
ATATAGAACCAACCGCTCCACTGGAAGTTTTATCCTCGTGGACGAGGGAACCAATTCCACCGTCGGCGCGGGAATGATTA
CCGGAGTCGGAGCCTAATTC

Downstream 100 bases:

>100_bases
GTCGAAAATATCCCGCTTTCTTGAATTTAGAAAATAAGAATATTCTATTGATCGGTGGAGGGAAAGTGGCTTTTGAAAAG
TTACCTCATCTCATCGATTC

Product: uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Precorrin-2 dehydrogenase; Sirohydrochlorin ferrochelatase [H]

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MLNADTDKDLKPGKVYLVGAGPGNPEDLTLRAYRILAKAEVILYDALLDPSFLEVFPKDAVIHYVGKRSGAHSATQEEIN
RLLLSYALQGKNVVRLKGGDPFVFGRGGEELITLINHNITYEIIPGVSSLNAGSSFAGFPLTHRGLSRQVLIMDGHTVLS
EETDWTWFAKFKGTIALFMGTASLPKIAKLLLQHGSDPDLPVALVENASLENCNIQTCALKDAADFCLEKKAKGPGIIYI
GKVVRFLENKRSISTFSQDLGGTRFDQVFSEIQGKKE

Sequences:

>Translated_277_residues
MLNADTDKDLKPGKVYLVGAGPGNPEDLTLRAYRILAKAEVILYDALLDPSFLEVFPKDAVIHYVGKRSGAHSATQEEIN
RLLLSYALQGKNVVRLKGGDPFVFGRGGEELITLINHNITYEIIPGVSSLNAGSSFAGFPLTHRGLSRQVLIMDGHTVLS
EETDWTWFAKFKGTIALFMGTASLPKIAKLLLQHGSDPDLPVALVENASLENCNIQTCALKDAADFCLEKKAKGPGIIYI
GKVVRFLENKRSISTFSQDLGGTRFDQVFSEIQGKKE
>Mature_277_residues
MLNADTDKDLKPGKVYLVGAGPGNPEDLTLRAYRILAKAEVILYDALLDPSFLEVFPKDAVIHYVGKRSGAHSATQEEIN
RLLLSYALQGKNVVRLKGGDPFVFGRGGEELITLINHNITYEIIPGVSSLNAGSSFAGFPLTHRGLSRQVLIMDGHTVLS
EETDWTWFAKFKGTIALFMGTASLPKIAKLLLQHGSDPDLPVALVENASLENCNIQTCALKDAADFCLEKKAKGPGIIYI
GKVVRFLENKRSISTFSQDLGGTRFDQVFSEIQGKKE

Specific function: Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into si

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=232, Percent_Identity=40.948275862069, Blast_Score=187, Evalue=9e-49,
Organism=Saccharomyces cerevisiae, GI6322922, Length=244, Percent_Identity=33.1967213114754, Blast_Score=117, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR016040
- InterPro:   IPR019478
- InterPro:   IPR006367
- InterPro:   IPR003043 [H]

Pfam domain/function: PF10414 CysG_dimeriser; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =1.3.1.76; =4.99.1.4 [H]

Molecular weight: Translated: 30266; Mature: 30266

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNADTDKDLKPGKVYLVGAGPGNPEDLTLRAYRILAKAEVILYDALLDPSFLEVFPKDA
CCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHEEEEEHHCCCHHHHHCCCCC
VIHYVGKRSGAHSATQEEINRLLLSYALQGKNVVRLKGGDPFVFGRGGEELITLINHNIT
EEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCEEEECCCHHHHHHHHCCCEE
YEIIPGVSSLNAGSSFAGFPLTHRGLSRQVLIMDGHTVLSEETDWTWFAKFKGTIALFMG
EEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCEEECCCCCCEEEEEECCEEEEEEC
TASLPKIAKLLLQHGSDPDLPVALVENASLENCNIQTCALKDAADFCLEKKAKGPGIIYI
CCCHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCEEEEECHHHHHHHHHHCCCCCCEEEH
GKVVRFLENKRSISTFSQDLGGTRFDQVFSEIQGKKE
HHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MLNADTDKDLKPGKVYLVGAGPGNPEDLTLRAYRILAKAEVILYDALLDPSFLEVFPKDA
CCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHEEEEEHHCCCHHHHHCCCCC
VIHYVGKRSGAHSATQEEINRLLLSYALQGKNVVRLKGGDPFVFGRGGEELITLINHNIT
EEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCEEEECCCHHHHHHHHCCCEE
YEIIPGVSSLNAGSSFAGFPLTHRGLSRQVLIMDGHTVLSEETDWTWFAKFKGTIALFMG
EEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCEEECCCCCCEEEEEECCEEEEEEC
TASLPKIAKLLLQHGSDPDLPVALVENASLENCNIQTCALKDAADFCLEKKAKGPGIIYI
CCCHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCEEEEECHHHHHHHHHHCCCCCCEEEH
GKVVRFLENKRSISTFSQDLGGTRFDQVFSEIQGKKE
HHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA