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The map label for this gene is murC-2 [H]

Identifier: 116326999

GI number: 116326999

Start: 199677

End: 201074

Strand: Reverse

Name: murC-2 [H]

Synonym: LBL_0169

Alternate gene names: 116326999

Gene position: 201074-199677 (Counterclockwise)

Preceding gene: 116327000

Following gene: 116326998

Centisome position: 5.56

GC content: 41.06

Gene sequence:

>1398_bases
TTGAAAATTTTTCTGATCGGAATCGGCGGGATCGCTATGGGCAATCTTGCTTATATGCTTCGAAAAAACGGACATGAAGT
TTCCGGCTCAGACACGGGCATTTATCCGCCGATGTCCGATAAATTAAAAGAATGGAATATTCCATACTTCGAAGGATTTA
AAGCCGAGAATGTTCAAGGCCAAGATTTGATCATCGTGGGGAACGCAATTTCCAGAGGAAATCCCGAAGTCGAAGAGACG
CTCAATTCGGGACTAAATTATCTTTCCATGCCTGCGGCAATCGGCGAATTTTTTCTCAAAGGAAAAAAAGTGATCGTAGT
CGCGGGAACACACGGAAAGACCACTACTACATTCTTAATTCATCATATACTTAAAGAAAATGGAATCGAGCCCGGCTTGT
TTGTCGGAGGAATCCGTAAGGACGGCTTTCCAGGGTTCGAACTTGGAAACGGATCTTATTTTGTCATCGAAGGGGACGAA
TATGATACCGCGTTTTTCGATAAATCTTCGAAATTTTTACACTATAGACCGACTTATGCGGTGTTAAACGCGTTGGATTT
TGATCATGCGGATATTTTTCCCGATATTTCCGCAATTGAAACCATGTTCAAACGTCTGATCAATTTGGTTCCCGGAAACG
GTAAAGTTTTCTATTGGAGCGGTTCAGGCTCCCTTAAAAAAATGATCCAAAACGTCAAGTTTACGAAAGTGGAAGGTTTT
GAGTGGAATCGAAAAGATTCTTTTCTTACTTGGAAAAAACACGAACTTTTTTTGGGAGATCAAAAACTAAATCCGGTTCT
TTTCGGAGATCACAATTACAGAAACATAGAGGTTGCGATTCGTGTTTGTTCCGAAATTCTCAAAACACAAAATCCTTCCG
ATTACAAACAGGGAATCGCAAAGGCAATCGATTCCTTTCCGGGAGTTAAAAGAAGACAGGATATTCTTTTTCAATCTCCG
ACCGCGATTGTGATGGAAGATTTCGCACATCACCCGGTTGCGGTTCACGAAACGATTCATGCGGTCAAAAAAAGATTTCA
AGGTCATAAAATTATTTCCTTATTTGAACCGAGAAGTGCAACTTCTCACCGAAACGTTTTTCAGAAGGAATATTCATATT
CCTTTATAGGTTCCGATCTAACCATTTTGACCGAGATTCATAATCTGAAGAAGGTTTCTAAAGACATTCGTTTGGACGTG
AAAAAGCTTGTTCAGAAACTTTTGAAGAACTCCAAAACAATTCCGGTTTATGCGAAGGACCCCGCAGAGCTGTTGGCAAA
ACTTGAGAAAATGATTCCCCAATTTACAGGACAGAAAATTCTGATCCTAGCCATGTCCAACGGATCGTTCGGAGGGATTT
ATCCCGGGTTGGTCGAATTGGCGCGAAAACACGTATGA

Upstream 100 bases:

>100_bases
ATAAGGATATCTTAATATTCTTTTTGTCCGGTTCTGCCTATTTTTTAACGGGAACCTTATTTTTAACGGTCGTTTATAAT
TTAGTTAGGACCATTCTCAA

Downstream 100 bases:

>100_bases
ACTTATCCGAGGAGCTAGATTCCATTTATCAAGAAGCCATTCAAAAAATTGGCTCTTCCATATCCGAAGAAGATTTAGAT
AAAAATAAAAACGATTTTAT

Product: UDP-N-acetylmuramate--L-alanine ligase

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MKIFLIGIGGIAMGNLAYMLRKNGHEVSGSDTGIYPPMSDKLKEWNIPYFEGFKAENVQGQDLIIVGNAISRGNPEVEET
LNSGLNYLSMPAAIGEFFLKGKKVIVVAGTHGKTTTTFLIHHILKENGIEPGLFVGGIRKDGFPGFELGNGSYFVIEGDE
YDTAFFDKSSKFLHYRPTYAVLNALDFDHADIFPDISAIETMFKRLINLVPGNGKVFYWSGSGSLKKMIQNVKFTKVEGF
EWNRKDSFLTWKKHELFLGDQKLNPVLFGDHNYRNIEVAIRVCSEILKTQNPSDYKQGIAKAIDSFPGVKRRQDILFQSP
TAIVMEDFAHHPVAVHETIHAVKKRFQGHKIISLFEPRSATSHRNVFQKEYSYSFIGSDLTILTEIHNLKKVSKDIRLDV
KKLVQKLLKNSKTIPVYAKDPAELLAKLEKMIPQFTGQKILILAMSNGSFGGIYPGLVELARKHV

Sequences:

>Translated_465_residues
MKIFLIGIGGIAMGNLAYMLRKNGHEVSGSDTGIYPPMSDKLKEWNIPYFEGFKAENVQGQDLIIVGNAISRGNPEVEET
LNSGLNYLSMPAAIGEFFLKGKKVIVVAGTHGKTTTTFLIHHILKENGIEPGLFVGGIRKDGFPGFELGNGSYFVIEGDE
YDTAFFDKSSKFLHYRPTYAVLNALDFDHADIFPDISAIETMFKRLINLVPGNGKVFYWSGSGSLKKMIQNVKFTKVEGF
EWNRKDSFLTWKKHELFLGDQKLNPVLFGDHNYRNIEVAIRVCSEILKTQNPSDYKQGIAKAIDSFPGVKRRQDILFQSP
TAIVMEDFAHHPVAVHETIHAVKKRFQGHKIISLFEPRSATSHRNVFQKEYSYSFIGSDLTILTEIHNLKKVSKDIRLDV
KKLVQKLLKNSKTIPVYAKDPAELLAKLEKMIPQFTGQKILILAMSNGSFGGIYPGLVELARKHV
>Mature_465_residues
MKIFLIGIGGIAMGNLAYMLRKNGHEVSGSDTGIYPPMSDKLKEWNIPYFEGFKAENVQGQDLIIVGNAISRGNPEVEET
LNSGLNYLSMPAAIGEFFLKGKKVIVVAGTHGKTTTTFLIHHILKENGIEPGLFVGGIRKDGFPGFELGNGSYFVIEGDE
YDTAFFDKSSKFLHYRPTYAVLNALDFDHADIFPDISAIETMFKRLINLVPGNGKVFYWSGSGSLKKMIQNVKFTKVEGF
EWNRKDSFLTWKKHELFLGDQKLNPVLFGDHNYRNIEVAIRVCSEILKTQNPSDYKQGIAKAIDSFPGVKRRQDILFQSP
TAIVMEDFAHHPVAVHETIHAVKKRFQGHKIISLFEPRSATSHRNVFQKEYSYSFIGSDLTILTEIHNLKKVSKDIRLDV
KKLVQKLLKNSKTIPVYAKDPAELLAKLEKMIPQFTGQKILILAMSNGSFGGIYPGLVELARKHV

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=473, Percent_Identity=35.7293868921776, Blast_Score=275, Evalue=6e-75,
Organism=Escherichia coli, GI1786279, Length=465, Percent_Identity=24.7311827956989, Blast_Score=125, Evalue=5e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 52233; Mature: 52233

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIFLIGIGGIAMGNLAYMLRKNGHEVSGSDTGIYPPMSDKLKEWNIPYFEGFKAENVQG
CEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCHHCCCCCCCCCC
QDLIIVGNAISRGNPEVEETLNSGLNYLSMPAAIGEFFLKGKKVIVVAGTHGKTTTTFLI
CEEEEECCCCCCCCCHHHHHHHCCCCHHEHHHHHHHHHHCCCEEEEEECCCCCHHHHHHH
HHILKENGIEPGLFVGGIRKDGFPGFELGNGSYFVIEGDEYDTAFFDKSSKFLHYRPTYA
HHHHHHCCCCCCEEEECCCCCCCCCEEECCCEEEEEECCCCCCHHCCCCCCEEEECCHHH
VLNALDFDHADIFPDISAIETMFKRLINLVPGNGKVFYWSGSGSLKKMIQNVKFTKVEGF
HHHHCCCCCHHCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHCCCEEEECCC
EWNRKDSFLTWKKHELFLGDQKLNPVLFGDHNYRNIEVAIRVCSEILKTQNPSDYKQGIA
CCCCCCCEEEEECCEEEECCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHH
KAIDSFPGVKRRQDILFQSPTAIVMEDFAHHPVAVHETIHAVKKRFQGHKIISLFEPRSA
HHHHHCCCCCHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCC
TSHRNVFQKEYSYSFIGSDLTILTEIHNLKKVSKDIRLDVKKLVQKLLKNSKTIPVYAKD
CHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
PAELLAKLEKMIPQFTGQKILILAMSNGSFGGIYPGLVELARKHV
HHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MKIFLIGIGGIAMGNLAYMLRKNGHEVSGSDTGIYPPMSDKLKEWNIPYFEGFKAENVQG
CEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCHHCCCCCCCCCC
QDLIIVGNAISRGNPEVEETLNSGLNYLSMPAAIGEFFLKGKKVIVVAGTHGKTTTTFLI
CEEEEECCCCCCCCCHHHHHHHCCCCHHEHHHHHHHHHHCCCEEEEEECCCCCHHHHHHH
HHILKENGIEPGLFVGGIRKDGFPGFELGNGSYFVIEGDEYDTAFFDKSSKFLHYRPTYA
HHHHHHCCCCCCEEEECCCCCCCCCEEECCCEEEEEECCCCCCHHCCCCCCEEEECCHHH
VLNALDFDHADIFPDISAIETMFKRLINLVPGNGKVFYWSGSGSLKKMIQNVKFTKVEGF
HHHHCCCCCHHCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHCCCEEEECCC
EWNRKDSFLTWKKHELFLGDQKLNPVLFGDHNYRNIEVAIRVCSEILKTQNPSDYKQGIA
CCCCCCCEEEEECCEEEECCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHH
KAIDSFPGVKRRQDILFQSPTAIVMEDFAHHPVAVHETIHAVKKRFQGHKIISLFEPRSA
HHHHHCCCCCHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCC
TSHRNVFQKEYSYSFIGSDLTILTEIHNLKKVSKDIRLDVKKLVQKLLKNSKTIPVYAKD
CHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
PAELLAKLEKMIPQFTGQKILILAMSNGSFGGIYPGLVELARKHV
HHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]