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The map label for this gene is pheS

Identifier: 116326998

GI number: 116326998

Start: 198655

End: 199680

Strand: Reverse

Name: pheS

Synonym: LBL_0168

Alternate gene names: 116326998

Gene position: 199680-198655 (Counterclockwise)

Preceding gene: 116326999

Following gene: 116326997

Centisome position: 5.52

GC content: 42.88

Gene sequence:

>1026_bases
ATGAACTTATCCGAGGAGCTAGATTCCATTTATCAAGAAGCCATTCAAAAAATTGGCTCTTCCATATCCGAAGAAGATTT
AGATAAAAATAAAAACGATTTTATCGGTAAGAAAGGAAAGCTAACCGCAGTTCTCAAGAATGTAGCTTTACTTTCCATCG
AAGAAAAAAAGACCATAGGCCAAAAAGCCAACGAACTTTCAAAAAAGCTCGAGAATTTCGTCGTGGAGACAAAGTCTTCC
CTTAAGAAAAAACTTTTTGAAACACAGGCCGCTTCCGAGTTTTTCGATTCGCTTCGTCCTCTTCCAAACGCTTCTAATGG
AAGTCTTCACCCGATTACTCAGATTCAATACGAGATCGAAGACATCTTTACATCGATGGGTTTCAGCATCATGGATGGTC
CTGAAATTGAAACCGACACGAACAACTTCGGCGCGTTAAATTTTACGGACGATCATCCTGCGAGAGAAATGCAGGATACG
TTTTATCTGGAAAACGGAAATCTTCTGAGAACTCACACTTCCGCAATTCAAGTAAGAACATTAAGAAAGCTGAAACCTCC
GTTCCGGATCATCGCTCCCGGAAGAGTGTTCCGTTACGAAGAAGTGGATGCTTCTCACGAACATACTTTTTATCAGATCG
AAGGAATGGTCGTAGGTAAGGATATCTCAGCCGCTAACCTGATCGATACGATGCAGGTCCTTCTCTCTAGAATCTTCGAA
AAAGAAATCAAAACCAGATTGCGACCGGGATACTTTCCTTTTGTGGAACCAGGTTTCGAATTGGATATCAACTGTCTTGT
GTGCGAAGGGAAAGGTTGTCCGGTTTGTAAACAATCGGGCTGGTTGGAACTTTTACCCTGCGGTTTGATTCATCCGAACG
TTCTTTCTCACGCGGGGCTCGATCCGAAAGAATGGACCGGCTTTGCGTTCGGACTCGGGCTCGACCGACTTGTGATGATG
CGGTACGGAATCCACGATATCCGTTACTTCCAATCGGGTAATCTAAGATTTTTAAAACAATTCTAA

Upstream 100 bases:

>100_bases
TTCCCCAATTTACAGGACAGAAAATTCTGATCCTAGCCATGTCCAACGGATCGTTCGGAGGGATTTATCCCGGGTTGGTC
GAATTGGCGCGAAAACACGT

Downstream 100 bases:

>100_bases
TATGATTTCCACTCCGATTCAGACTCGTTGGATGGATATGGATCCGTTCGCTCATGTCAGCAATTCCGTTTTTGTTTCTT
ATCTTGAGATTGGAAGAGTG

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MNLSEELDSIYQEAIQKIGSSISEEDLDKNKNDFIGKKGKLTAVLKNVALLSIEEKKTIGQKANELSKKLENFVVETKSS
LKKKLFETQAASEFFDSLRPLPNASNGSLHPITQIQYEIEDIFTSMGFSIMDGPEIETDTNNFGALNFTDDHPAREMQDT
FYLENGNLLRTHTSAIQVRTLRKLKPPFRIIAPGRVFRYEEVDASHEHTFYQIEGMVVGKDISAANLIDTMQVLLSRIFE
KEIKTRLRPGYFPFVEPGFELDINCLVCEGKGCPVCKQSGWLELLPCGLIHPNVLSHAGLDPKEWTGFAFGLGLDRLVMM
RYGIHDIRYFQSGNLRFLKQF

Sequences:

>Translated_341_residues
MNLSEELDSIYQEAIQKIGSSISEEDLDKNKNDFIGKKGKLTAVLKNVALLSIEEKKTIGQKANELSKKLENFVVETKSS
LKKKLFETQAASEFFDSLRPLPNASNGSLHPITQIQYEIEDIFTSMGFSIMDGPEIETDTNNFGALNFTDDHPAREMQDT
FYLENGNLLRTHTSAIQVRTLRKLKPPFRIIAPGRVFRYEEVDASHEHTFYQIEGMVVGKDISAANLIDTMQVLLSRIFE
KEIKTRLRPGYFPFVEPGFELDINCLVCEGKGCPVCKQSGWLELLPCGLIHPNVLSHAGLDPKEWTGFAFGLGLDRLVMM
RYGIHDIRYFQSGNLRFLKQF
>Mature_341_residues
MNLSEELDSIYQEAIQKIGSSISEEDLDKNKNDFIGKKGKLTAVLKNVALLSIEEKKTIGQKANELSKKLENFVVETKSS
LKKKLFETQAASEFFDSLRPLPNASNGSLHPITQIQYEIEDIFTSMGFSIMDGPEIETDTNNFGALNFTDDHPAREMQDT
FYLENGNLLRTHTSAIQVRTLRKLKPPFRIIAPGRVFRYEEVDASHEHTFYQIEGMVVGKDISAANLIDTMQVLLSRIFE
KEIKTRLRPGYFPFVEPGFELDINCLVCEGKGCPVCKQSGWLELLPCGLIHPNVLSHAGLDPKEWTGFAFGLGLDRLVMM
RYGIHDIRYFQSGNLRFLKQF

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5729820, Length=283, Percent_Identity=30.0353356890459, Blast_Score=118, Evalue=9e-27,
Organism=Homo sapiens, GI4758340, Length=273, Percent_Identity=29.6703296703297, Blast_Score=110, Evalue=2e-24,
Organism=Escherichia coli, GI1788007, Length=335, Percent_Identity=40.2985074626866, Blast_Score=273, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI32563657, Length=280, Percent_Identity=32.8571428571429, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17508957, Length=280, Percent_Identity=32.8571428571429, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI32566635, Length=256, Percent_Identity=32.03125, Blast_Score=113, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6321087, Length=268, Percent_Identity=31.3432835820896, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6325304, Length=314, Percent_Identity=26.1146496815287, Blast_Score=106, Evalue=4e-24,
Organism=Drosophila melanogaster, GI18858079, Length=280, Percent_Identity=30, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI17137424, Length=289, Percent_Identity=27.681660899654, Blast_Score=98, Evalue=7e-21,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_LEPBJ (Q04P74)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_802054.1
- ProteinModelPortal:   Q04P74
- SMR:   Q04P74
- STRING:   Q04P74
- GeneID:   4411973
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_2895
- eggNOG:   COG0016
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- PhylomeDB:   Q04P74
- ProtClustDB:   PRK00488
- BioCyc:   LBOR355277:LBJ_2895-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 38666; Mature: 38666

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLSEELDSIYQEAIQKIGSSISEEDLDKNKNDFIGKKGKLTAVLKNVALLSIEEKKTIG
CCHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHCCCCCHHHHHHHHHHHEEEHHHHHHH
QKANELSKKLENFVVETKSSLKKKLFETQAASEFFDSLRPLPNASNGSLHPITQIQYEIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
DIFTSMGFSIMDGPEIETDTNNFGALNFTDDHPAREMQDTFYLENGNLLRTHTSAIQVRT
HHHHHCCCEECCCCCCCCCCCCCCEEECCCCCCHHHHHHEEEEECCCEEEEHHHHHHHHH
LRKLKPPFRIIAPGRVFRYEEVDASHEHTFYQIEGMVVGKDISAANLIDTMQVLLSRIFE
HHHCCCCEEEECCCCEEEECCCCCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHH
KEIKTRLRPGYFPFVEPGFELDINCLVCEGKGCPVCKQSGWLELLPCGLIHPNVLSHAGL
HHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCEEEECCCCCCCHHHHHCCC
DPKEWTGFAFGLGLDRLVMMRYGIHDIRYFQSGNLRFLKQF
CHHHHCCCHHHHHHHHHHHHHHCHHHHEEEECCCEEEEECC
>Mature Secondary Structure
MNLSEELDSIYQEAIQKIGSSISEEDLDKNKNDFIGKKGKLTAVLKNVALLSIEEKKTIG
CCHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHCCCCCHHHHHHHHHHHEEEHHHHHHH
QKANELSKKLENFVVETKSSLKKKLFETQAASEFFDSLRPLPNASNGSLHPITQIQYEIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
DIFTSMGFSIMDGPEIETDTNNFGALNFTDDHPAREMQDTFYLENGNLLRTHTSAIQVRT
HHHHHCCCEECCCCCCCCCCCCCCEEECCCCCCHHHHHHEEEEECCCEEEEHHHHHHHHH
LRKLKPPFRIIAPGRVFRYEEVDASHEHTFYQIEGMVVGKDISAANLIDTMQVLLSRIFE
HHHCCCCEEEECCCCEEEECCCCCCCCCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHH
KEIKTRLRPGYFPFVEPGFELDINCLVCEGKGCPVCKQSGWLELLPCGLIHPNVLSHAGL
HHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCEEEECCCCCCCHHHHHCCC
DPKEWTGFAFGLGLDRLVMMRYGIHDIRYFQSGNLRFLKQF
CHHHHCCCHHHHHHHHHHHHHHCHHHHEEEECCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA