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The map label for this gene is hycE [C]

Identifier: 116326950

GI number: 116326950

Start: 132898

End: 134307

Strand: Reverse

Name: hycE [C]

Synonym: LBL_0116

Alternate gene names: 116326950

Gene position: 134307-132898 (Counterclockwise)

Preceding gene: 116326951

Following gene: 116326949

Centisome position: 3.72

GC content: 43.33

Gene sequence:

>1410_bases
ATGCGTAGCGTGACTGGTTTATACCCGAGAAAAGGTAAAAAGGGTTACAACCGCTTTTGGCTTACAAGCGACGGAGTAGA
ACATGAAATCGTAGAATATTCCGAAAAGGAAGCCTTTGAGGATACCGCGAATCCGATTTGGATTCTCAGACATTCTCTTG
GAACCGACCAGGGAGAAGAGGATTACTCTTCTTATGATTTCGAAAGATATATTACGGCGGACCGCAAAACCGATCTGGAA
AAATTCGTAACTAAAAAAGGTATTAAGGATTTAGTATATAAGGGATTAAAGGTACCGATTCCCGCGAGTCATTATTCTCA
CGCGGTTGGACCGATTCATGCGGGAGTAATCGAGCCCGGGCATTTTCGTTTTATCGTAAAAGGAGAAGAGATTCGAAATT
TAGACATTCGACTCGGTTTTCAAAAACGAGGTTTAATCGATTTAATCAAGGGAAAAGGATCGAAGGATTCTTTTCCCTAT
GCGGAAGCTATTTCGGGAGATAGTACAATTGCTTATGCGATTGCGTTTTCCAGAATTTTCGAGGAAGCACAGAAAATTGC
CGTTCCTCAAGAATTGGATTTTGCAAGACTCGTTCTTTTGGAACTTGAAAGAATTGCGACTCATATCGGAGATATGGGTG
CGATTGCGGGTGACGTAGGTTATTATCCTTTACAAGGTGTTTGTTCCTTGCAAAGAGGAGTTCCTCTTGGAGTGATGGAG
GCTTTGACCGGAAATCGTTTCGGAAGAGGAGCGTTATCTCCAGGTAAGATCGTTCTTAGAAAGGGACTCAACCGTGAAAA
ATTAAACGAACTCGCAGAAAGGATTCGCTCGGTTACTGCGGATGTGGTCGCACATTTTGAACGTGCTGCGAACGTTTCTA
CGAACCGGGAGAGGCTGCAGTCCTGCGGGATAATCCGGCAAAAACAAGTTAGGCATCTTGGTTTTATAGGAATGGTGGAG
AAGTGCACAGGGCTCAGCAGGGATCTTCGTAGTTTAGAAAAATCGTATATGTTTAGCTCTCCGATTTCTCTTGAGTTGAA
TCGAGATCATATGAAAGGGGACGCATGGGCAAGGTTTTATTTACGTTGTGAAGAATTGAAAAACAGCGGTAAATGGCTCA
TAGATGCGATTCCTAAATTAGAAGCCGCTTTTTCCCTGAAATTTGGAAAAGTTCCGTCAAAATCCTCTGTCAAAAAAGGA
ACCTATTATTCCGCAGTGGAAGGATGGAGAGGACCGGTGCTAGTATCCTTGGATTTGGACGCAACCGGCTCTATTGAAAA
CGCTTACATCAGGGAAGTTTCCGTTCCTAATTGGCATGCACTTGAGTTAGCGGTAAGGGGAGAATATATCGGAGACTTCC
CGTTGAATAATAAGTCCTTCAATCTGAGCTATGTAGGAGTGGATCTATGA

Upstream 100 bases:

>100_bases
AACATTACAGATCCGAATGACCAATTCGCTTTTGCTTTTTATCATGGTACTGGGTCTCGGTATTTGGGGTTTCTATCATC
TACTGCAAGGAGAATTTTGA

Downstream 100 bases:

>100_bases
AAATTATATTCGAGATAATGAATATAATTCGTCCTGCAAAAACGATGAATTATAAAAAGGTTTTACCCTTCAACCCGAAC
GCGAGGGGAATTCCGATTCC

Product: metal (Ni/Fe) hydrogenase, large subunit

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MRSVTGLYPRKGKKGYNRFWLTSDGVEHEIVEYSEKEAFEDTANPIWILRHSLGTDQGEEDYSSYDFERYITADRKTDLE
KFVTKKGIKDLVYKGLKVPIPASHYSHAVGPIHAGVIEPGHFRFIVKGEEIRNLDIRLGFQKRGLIDLIKGKGSKDSFPY
AEAISGDSTIAYAIAFSRIFEEAQKIAVPQELDFARLVLLELERIATHIGDMGAIAGDVGYYPLQGVCSLQRGVPLGVME
ALTGNRFGRGALSPGKIVLRKGLNREKLNELAERIRSVTADVVAHFERAANVSTNRERLQSCGIIRQKQVRHLGFIGMVE
KCTGLSRDLRSLEKSYMFSSPISLELNRDHMKGDAWARFYLRCEELKNSGKWLIDAIPKLEAAFSLKFGKVPSKSSVKKG
TYYSAVEGWRGPVLVSLDLDATGSIENAYIREVSVPNWHALELAVRGEYIGDFPLNNKSFNLSYVGVDL

Sequences:

>Translated_469_residues
MRSVTGLYPRKGKKGYNRFWLTSDGVEHEIVEYSEKEAFEDTANPIWILRHSLGTDQGEEDYSSYDFERYITADRKTDLE
KFVTKKGIKDLVYKGLKVPIPASHYSHAVGPIHAGVIEPGHFRFIVKGEEIRNLDIRLGFQKRGLIDLIKGKGSKDSFPY
AEAISGDSTIAYAIAFSRIFEEAQKIAVPQELDFARLVLLELERIATHIGDMGAIAGDVGYYPLQGVCSLQRGVPLGVME
ALTGNRFGRGALSPGKIVLRKGLNREKLNELAERIRSVTADVVAHFERAANVSTNRERLQSCGIIRQKQVRHLGFIGMVE
KCTGLSRDLRSLEKSYMFSSPISLELNRDHMKGDAWARFYLRCEELKNSGKWLIDAIPKLEAAFSLKFGKVPSKSSVKKG
TYYSAVEGWRGPVLVSLDLDATGSIENAYIREVSVPNWHALELAVRGEYIGDFPLNNKSFNLSYVGVDL
>Mature_469_residues
MRSVTGLYPRKGKKGYNRFWLTSDGVEHEIVEYSEKEAFEDTANPIWILRHSLGTDQGEEDYSSYDFERYITADRKTDLE
KFVTKKGIKDLVYKGLKVPIPASHYSHAVGPIHAGVIEPGHFRFIVKGEEIRNLDIRLGFQKRGLIDLIKGKGSKDSFPY
AEAISGDSTIAYAIAFSRIFEEAQKIAVPQELDFARLVLLELERIATHIGDMGAIAGDVGYYPLQGVCSLQRGVPLGVME
ALTGNRFGRGALSPGKIVLRKGLNREKLNELAERIRSVTADVVAHFERAANVSTNRERLQSCGIIRQKQVRHLGFIGMVE
KCTGLSRDLRSLEKSYMFSSPISLELNRDHMKGDAWARFYLRCEELKNSGKWLIDAIPKLEAAFSLKFGKVPSKSSVKKG
TYYSAVEGWRGPVLVSLDLDATGSIENAYIREVSVPNWHALELAVRGEYIGDFPLNNKSFNLSYVGVDL

Specific function: Unknown

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 49 kDa subunit family [H]

Homologues:

Organism=Escherichia coli, GI1788832, Length=372, Percent_Identity=24.7311827956989, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1789076, Length=380, Percent_Identity=22.6315789473684, Blast_Score=90, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24638644, Length=372, Percent_Identity=24.7311827956989, Blast_Score=73, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR014029
- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 52518; Mature: 52518

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSVTGLYPRKGKKGYNRFWLTSDGVEHEIVEYSEKEAFEDTANPIWILRHSLGTDQGEE
CCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCC
DYSSYDFERYITADRKTDLEKFVTKKGIKDLVYKGLKVPIPASHYSHAVGPIHAGVIEPG
HHHCCCHHHHEECCCHHHHHHHHHHHCHHHHHHCCCCCCCCCHHCCCCCCCCCCCCCCCC
HFRFIVKGEEIRNLDIRLGFQKRGLIDLIKGKGSKDSFPYAEAISGDSTIAYAIAFSRIF
CEEEEEECCCEEEEEEEECCCCCCCHHHHCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHH
EEAQKIAVPQELDFARLVLLELERIATHIGDMGAIAGDVGYYPLQGVCSLQRGVPLGVME
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHCCCCHHHHH
ALTGNRFGRGALSPGKIVLRKGLNREKLNELAERIRSVTADVVAHFERAANVSTNRERLQ
HHHCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
SCGIIRQKQVRHLGFIGMVEKCTGLSRDLRSLEKSYMFSSPISLELNRDHMKGDAWARFY
HCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHH
LRCEELKNSGKWLIDAIPKLEAAFSLKFGKVPSKSSVKKGTYYSAVEGWRGPVLVSLDLD
HHHHHHHCCCCEEEEHHHHHHHHHEEEECCCCCCCCCCCCCCHHHHCCCCCCEEEEEECC
ATGSIENAYIREVSVPNWHALELAVRGEYIGDFPLNNKSFNLSYVGVDL
CCCCCCCCEEEEECCCCCEEEEEEEECCEECCCCCCCCCEEEEEEEECC
>Mature Secondary Structure
MRSVTGLYPRKGKKGYNRFWLTSDGVEHEIVEYSEKEAFEDTANPIWILRHSLGTDQGEE
CCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCC
DYSSYDFERYITADRKTDLEKFVTKKGIKDLVYKGLKVPIPASHYSHAVGPIHAGVIEPG
HHHCCCHHHHEECCCHHHHHHHHHHHCHHHHHHCCCCCCCCCHHCCCCCCCCCCCCCCCC
HFRFIVKGEEIRNLDIRLGFQKRGLIDLIKGKGSKDSFPYAEAISGDSTIAYAIAFSRIF
CEEEEEECCCEEEEEEEECCCCCCCHHHHCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHH
EEAQKIAVPQELDFARLVLLELERIATHIGDMGAIAGDVGYYPLQGVCSLQRGVPLGVME
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHCCCCHHHHH
ALTGNRFGRGALSPGKIVLRKGLNREKLNELAERIRSVTADVVAHFERAANVSTNRERLQ
HHHCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
SCGIIRQKQVRHLGFIGMVEKCTGLSRDLRSLEKSYMFSSPISLELNRDHMKGDAWARFY
HCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHH
LRCEELKNSGKWLIDAIPKLEAAFSLKFGKVPSKSSVKKGTYYSAVEGWRGPVLVSLDLD
HHHHHHHCCCCEEEEHHHHHHHHHEEEECCCCCCCCCCCCCCHHHHCCCCCCEEEEEECC
ATGSIENAYIREVSVPNWHALELAVRGEYIGDFPLNNKSFNLSYVGVDL
CCCCCCCCEEEEECCCCCEEEEEEEECCEECCCCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 4Fe-4S Cluster. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]