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The map label for this gene is hycG [H]

Identifier: 116326949

GI number: 116326949

Start: 132080

End: 132901

Strand: Reverse

Name: hycG [H]

Synonym: LBL_0115

Alternate gene names: 116326949

Gene position: 132901-132080 (Counterclockwise)

Preceding gene: 116326950

Following gene: 116326947

Centisome position: 3.68

GC content: 42.94

Gene sequence:

>822_bases
ATGAAAATTATATTCGAGATAATGAATATAATTCGTCCTGCAAAAACGATGAATTATAAAAAGGTTTTACCCTTCAACCC
GAACGCGAGGGGAATTCCGATTCCTGTGTTGTCTTCCAATGAGTCTTGTCTTTCCTGCAAGTCTTGCGAACAAGTATGTC
CGACTCATTCACTCAAGATTCATTCTAAGGATAAAATGAGTTTTGATTACGGAGCTTGTCTTCAGTGCGGAAGATGTTCC
GAGTTTTGCTCCGATAAAAAGATCATCGATTCCGGTTTTGTTCACGTATATTCCACCGATCGGGAAGCGTTAAAAGTAAC
GTATACCAATGGAATGCCGGATGAAAAACCGGAAATGGAAACCGAGGAAGTAAAACGATTTAGAAAAACTACAAAAAAAA
CCGGATTTCAGTTTAGGGAGGTCGCTGCTAGCGGAAATAATACGACCGAAGCGGAAATCAACGCAAGTTTTAACGCTCTT
TTTGATAGTGAGGCGAGTAAGATTCGAGTCGTCGCTTCCCCAAAGCATGCTGATGCACTTGTCTATTCAGGGCCTGTGGG
ACCGAACATGGAAGAGCCGCTTAATACGGCTTGGGAAACGATGCCTTCTCCAAAAGCTCTTGTGGCTTGCGGTTCGGAAG
CGGTTTCAGGTGGGCTTTTCAAACTTGGTAAACTTCCGAAAGAACCTGACTTGTTCATTGGAGGAGATCCCCCTCGTCCG
GATGTGATTATTTCCGCGTTTCGGTATTTGATGGGAACCAGAGAACTCTCTTTCACAGCGGAATTGGTAAAGTTCGTTCA
AAATTTAAAGAAAACCAAGTAA

Upstream 100 bases:

>100_bases
CTAATTGGCATGCACTTGAGTTAGCGGTAAGGGGAGAATATATCGGAGACTTCCCGTTGAATAATAAGTCCTTCAATCTG
AGCTATGTAGGAGTGGATCT

Downstream 100 bases:

>100_bases
TTTAAAATATTCTAATATGCTCGAAAACTGAGCCGGATTTAAAATTCGTATAGAATCGGAGCATATTAGAAAAATCCAAA
CAAAACCGTTATGAATTTAG

Product: metal (Ni/Fe) hydrogenase, small subunit

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 7; Hydrogenase-3 component G [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MKIIFEIMNIIRPAKTMNYKKVLPFNPNARGIPIPVLSSNESCLSCKSCEQVCPTHSLKIHSKDKMSFDYGACLQCGRCS
EFCSDKKIIDSGFVHVYSTDREALKVTYTNGMPDEKPEMETEEVKRFRKTTKKTGFQFREVAASGNNTTEAEINASFNAL
FDSEASKIRVVASPKHADALVYSGPVGPNMEEPLNTAWETMPSPKALVACGSEAVSGGLFKLGKLPKEPDLFIGGDPPRP
DVIISAFRYLMGTRELSFTAELVKFVQNLKKTK

Sequences:

>Translated_273_residues
MKIIFEIMNIIRPAKTMNYKKVLPFNPNARGIPIPVLSSNESCLSCKSCEQVCPTHSLKIHSKDKMSFDYGACLQCGRCS
EFCSDKKIIDSGFVHVYSTDREALKVTYTNGMPDEKPEMETEEVKRFRKTTKKTGFQFREVAASGNNTTEAEINASFNAL
FDSEASKIRVVASPKHADALVYSGPVGPNMEEPLNTAWETMPSPKALVACGSEAVSGGLFKLGKLPKEPDLFIGGDPPRP
DVIISAFRYLMGTRELSFTAELVKFVQNLKKTK
>Mature_273_residues
MKIIFEIMNIIRPAKTMNYKKVLPFNPNARGIPIPVLSSNESCLSCKSCEQVCPTHSLKIHSKDKMSFDYGACLQCGRCS
EFCSDKKIIDSGFVHVYSTDREALKVTYTNGMPDEKPEMETEEVKRFRKTTKKTGFQFREVAASGNNTTEAEINASFNAL
FDSEASKIRVVASPKHADALVYSGPVGPNMEEPLNTAWETMPSPKALVACGSEAVSGGLFKLGKLPKEPDLFIGGDPPRP
DVIISAFRYLMGTRELSFTAELVKFVQNLKKTK

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3260

COG function: function code C; Ni,Fe-hydrogenase III small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789074, Length=139, Percent_Identity=35.2517985611511, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788834, Length=141, Percent_Identity=34.0425531914894, Blast_Score=74, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: NA

Molecular weight: Translated: 30221; Mature: 30221

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIIFEIMNIIRPAKTMNYKKVLPFNPNARGIPIPVLSSNESCLSCKSCEQVCPTHSLKI
CHHHHHHHHHHCCHHHCCCCEECCCCCCCCCCEEEEECCCCCHHCHHHHHHHCCCCCEEE
HSKDKMSFDYGACLQCGRCSEFCSDKKIIDSGFVHVYSTDREALKVTYTNGMPDEKPEME
ECCCCCCCCHHHHHCCCCHHHHHCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCC
TEEVKRFRKTTKKTGFQFREVAASGNNTTEAEINASFNALFDSEASKIRVVASPKHADAL
HHHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEECCCHHHEECCCCCEEEEEECCCCCCEE
VYSGPVGPNMEEPLNTAWETMPSPKALVACGSEAVSGGLFKLGKLPKEPDLFIGGDPPRP
EEECCCCCCCHHHHHHHHHCCCCCHHEEEECCHHHCCCHHHHCCCCCCCCEEECCCCCCH
DVIISAFRYLMGTRELSFTAELVKFVQNLKKTK
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKIIFEIMNIIRPAKTMNYKKVLPFNPNARGIPIPVLSSNESCLSCKSCEQVCPTHSLKI
CHHHHHHHHHHCCHHHCCCCEECCCCCCCCCCEEEEECCCCCHHCHHHHHHHCCCCCEEE
HSKDKMSFDYGACLQCGRCSEFCSDKKIIDSGFVHVYSTDREALKVTYTNGMPDEKPEME
ECCCCCCCCHHHHHCCCCHHHHHCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCC
TEEVKRFRKTTKKTGFQFREVAASGNNTTEAEINASFNALFDSEASKIRVVASPKHADAL
HHHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEECCCHHHEECCCCCEEEEEECCCCCCEE
VYSGPVGPNMEEPLNTAWETMPSPKALVACGSEAVSGGLFKLGKLPKEPDLFIGGDPPRP
EEECCCCCCCHHHHHHHHHCCCCCHHEEEECCHHHCCCHHHHCCCCCCCCEEECCCCCCH
DVIISAFRYLMGTRELSFTAELVKFVQNLKKTK
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503 [H]