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The map label for this gene is dnaA [H]

Identifier: 116326856

GI number: 116326856

Start: 4254

End: 5567

Strand: Direct

Name: dnaA [H]

Synonym: LBL_0004

Alternate gene names: 116326856

Gene position: 4254-5567 (Clockwise)

Preceding gene: 116326855

Following gene: 116326857

Centisome position: 0.12

GC content: 36.0

Gene sequence:

>1314_bases
TTGAACCTAGCTTGGAACAAAATCTTGGAGGAAGTATCTAAAAAAATATCCCCTCAATACTATGAACGGTTCATTGATAC
TTTGAAATTAGAAACCCTTAACTCCGAAAAATGTACAATTATTGCTCCTTCTGCTACGATCAAAACTCATGTTGAAAGAA
AATATCAGAGTATAATCGAAAATGCAATCTTAGAAGCCTGCGGCGATAAAATCCCCGTTGAAATTCTGATCGAAACCAAG
GCGACTTCCCCTCTGCAGTCGTTTCTCGAAAAATCCTTTGATCAAAAAGATTTTCAATTTAATCCGGATTATACATTCGA
AACCTTTATCGTAGGCGACTGCAACCGTTTAGCATACACGGCCGCAAAGGAATGCGTTCGAAAACCTGCGGAAATAAATC
CCCTTTATCTGTTTGGTAGCGTAGGAGTGGGAAAAACACACTTACTTCATGCGATCGGATCCGAACTTATTAAAAAAGAC
CCTTGGAAAACGGTATGTTATATTGATATTTCTTCTTTTATGAATGAGTTTCGTTTTGCTCTTCAATCCAGAGAACTTAT
CGAAAGTTTCAAGATAAAATATCAGTCTTACAATTGTCTTCTTGTGGATGATATACAACTTCTTTCGACAAATGCCGAAA
AAACTCAGGATGAATTTTTTGCACTGTTTAATTTTCTTTTCGAAAGAAAAAGACAGATCGTAATCGCATCGGATCGCCCA
AGTTCCGAACTTACGATTCATGAAAGACTGAAATCCAGGTTTGTCACAGGTGTTCAAGCCGACATCCAATATCCCAACAA
AGAGATTCGAAAAGGAATCGTAACGTCCCATTCTAAAATTATGGATCTCGGTTTAAGCGAAGACGTATTAGAATTTTTGG
CGGATCAAATCGAAGAAGATACGAGACTTTTACTCGGCGCACTCAACGACATTTATTTATATAAAAAATCCTATTCGCTT
CTTTTTTTAAATTTAGATAAGGTAAAGGAAATCGTAAAAAACCGTCTTTATCGTAAAAAAAACGTGGAATTTTCACATGA
TCGGATTATTGAATCCGTTGCTAAAGAATTCAATTTGGATGCCGCTGAGATTATGGGCAAAAGCAGAAAAAAAGAATTAA
TCATTCCACGTCATATTTGTTTTTATTTGTTACACAATGTTTTTAACGTCAATAAATCCCAAGTCGGAAGACTCTTTCAA
ACTCAACATACAACAGTAATTCATGGACTTCGAAAAACCGAAGAACTTCTTTCTGACAATAAGGAAATACGTTTTCTAGT
GGAACGAATTAGTTCGAAGTACAAGCTCCAGTAA

Upstream 100 bases:

>100_bases
GATCGTTTTATTTGATTGAATTGGGAAAATATCTTCAATTTAAATTCGTACCAATCTTTTCGGGAGAAAATTTTATGTTT
TTTTATTTTTGGAGGATGTG

Downstream 100 bases:

>100_bases
AATATGTACTTTTAAACAAAGAATTTGTCCCAAAACCTTAAAAGAAAAAAGAAATCAGTACAATCATTTCATAAGTTTGT
AATAGAGTGTGGAAATTCCC

Product: chromosomal replication initiator protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MNLAWNKILEEVSKKISPQYYERFIDTLKLETLNSEKCTIIAPSATIKTHVERKYQSIIENAILEACGDKIPVEILIETK
ATSPLQSFLEKSFDQKDFQFNPDYTFETFIVGDCNRLAYTAAKECVRKPAEINPLYLFGSVGVGKTHLLHAIGSELIKKD
PWKTVCYIDISSFMNEFRFALQSRELIESFKIKYQSYNCLLVDDIQLLSTNAEKTQDEFFALFNFLFERKRQIVIASDRP
SSELTIHERLKSRFVTGVQADIQYPNKEIRKGIVTSHSKIMDLGLSEDVLEFLADQIEEDTRLLLGALNDIYLYKKSYSL
LFLNLDKVKEIVKNRLYRKKNVEFSHDRIIESVAKEFNLDAAEIMGKSRKKELIIPRHICFYLLHNVFNVNKSQVGRLFQ
TQHTTVIHGLRKTEELLSDNKEIRFLVERISSKYKLQ

Sequences:

>Translated_437_residues
MNLAWNKILEEVSKKISPQYYERFIDTLKLETLNSEKCTIIAPSATIKTHVERKYQSIIENAILEACGDKIPVEILIETK
ATSPLQSFLEKSFDQKDFQFNPDYTFETFIVGDCNRLAYTAAKECVRKPAEINPLYLFGSVGVGKTHLLHAIGSELIKKD
PWKTVCYIDISSFMNEFRFALQSRELIESFKIKYQSYNCLLVDDIQLLSTNAEKTQDEFFALFNFLFERKRQIVIASDRP
SSELTIHERLKSRFVTGVQADIQYPNKEIRKGIVTSHSKIMDLGLSEDVLEFLADQIEEDTRLLLGALNDIYLYKKSYSL
LFLNLDKVKEIVKNRLYRKKNVEFSHDRIIESVAKEFNLDAAEIMGKSRKKELIIPRHICFYLLHNVFNVNKSQVGRLFQ
TQHTTVIHGLRKTEELLSDNKEIRFLVERISSKYKLQ
>Mature_437_residues
MNLAWNKILEEVSKKISPQYYERFIDTLKLETLNSEKCTIIAPSATIKTHVERKYQSIIENAILEACGDKIPVEILIETK
ATSPLQSFLEKSFDQKDFQFNPDYTFETFIVGDCNRLAYTAAKECVRKPAEINPLYLFGSVGVGKTHLLHAIGSELIKKD
PWKTVCYIDISSFMNEFRFALQSRELIESFKIKYQSYNCLLVDDIQLLSTNAEKTQDEFFALFNFLFERKRQIVIASDRP
SSELTIHERLKSRFVTGVQADIQYPNKEIRKGIVTSHSKIMDLGLSEDVLEFLADQIEEDTRLLLGALNDIYLYKKSYSL
LFLNLDKVKEIVKNRLYRKKNVEFSHDRIIESVAKEFNLDAAEIMGKSRKKELIIPRHICFYLLHNVFNVNKSQVGRLFQ
TQHTTVIHGLRKTEELLSDNKEIRFLVERISSKYKLQ

Specific function: Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box):5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phosph

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family [H]

Homologues:

Organism=Escherichia coli, GI2367267, Length=455, Percent_Identity=28.5714285714286, Blast_Score=192, Evalue=5e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001957
- InterPro:   IPR020591
- InterPro:   IPR013159
- InterPro:   IPR013317
- InterPro:   IPR010921 [H]

Pfam domain/function: PF00308 Bac_DnaA; PF08299 Bac_DnaA_C [H]

EC number: NA

Molecular weight: Translated: 50815; Mature: 50815

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLAWNKILEEVSKKISPQYYERFIDTLKLETLNSEKCTIIAPSATIKTHVERKYQSIIE
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHH
NAILEACGDKIPVEILIETKATSPLQSFLEKSFDQKDFQFNPDYTFETFIVGDCNRLAYT
HHHHHHCCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCHHHHHH
AAKECVRKPAEINPLYLFGSVGVGKTHLLHAIGSELIKKDPWKTVCYIDISSFMNEFRFA
HHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHH
LQSRELIESFKIKYQSYNCLLVDDIQLLSTNAEKTQDEFFALFNFLFERKRQIVIASDRP
HHHHHHHHHHHHHHCCCCEEEEECHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEECCCC
SSELTIHERLKSRFVTGVQADIQYPNKEIRKGIVTSHSKIMDLGLSEDVLEFLADQIEED
CCCHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHH
TRLLLGALNDIYLYKKSYSLLFLNLDKVKEIVKNRLYRKKNVEFSHDRIIESVAKEFNLD
HHHHHHHHHHHEEEEECCEEEEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
AAEIMGKSRKKELIIPRHICFYLLHNVFNVNKSQVGRLFQTQHTTVIHGLRKTEELLSDN
HHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KEIRFLVERISSKYKLQ
HHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNLAWNKILEEVSKKISPQYYERFIDTLKLETLNSEKCTIIAPSATIKTHVERKYQSIIE
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHH
NAILEACGDKIPVEILIETKATSPLQSFLEKSFDQKDFQFNPDYTFETFIVGDCNRLAYT
HHHHHHCCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCHHHHHH
AAKECVRKPAEINPLYLFGSVGVGKTHLLHAIGSELIKKDPWKTVCYIDISSFMNEFRFA
HHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHH
LQSRELIESFKIKYQSYNCLLVDDIQLLSTNAEKTQDEFFALFNFLFERKRQIVIASDRP
HHHHHHHHHHHHHHCCCCEEEEECHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEECCCC
SSELTIHERLKSRFVTGVQADIQYPNKEIRKGIVTSHSKIMDLGLSEDVLEFLADQIEED
CCCHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHH
TRLLLGALNDIYLYKKSYSLLFLNLDKVKEIVKNRLYRKKNVEFSHDRIIESVAKEFNLD
HHHHHHHHHHHEEEEECCEEEEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
AAEIMGKSRKKELIIPRHICFYLLHNVFNVNKSQVGRLFQTQHTTVIHGLRKTEELLSDN
HHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KEIRFLVERISSKYKLQ
HHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA