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The map label for this gene is dnaN [H]

Identifier: 116326857

GI number: 116326857

Start: 6679

End: 7800

Strand: Direct

Name: dnaN [H]

Synonym: LBL_0005

Alternate gene names: 116326857

Gene position: 6679-7800 (Clockwise)

Preceding gene: 116326856

Following gene: 116326858

Centisome position: 0.18

GC content: 36.81

Gene sequence:

>1122_bases
TTGAAAATTAAAGTTAATACATCTGATTTTTTAAAAGCAATTCATGCAGTGGAAGGTGTAATCTCTGCACGAGAAATTAA
ATCTATATTATCAAATCTTAAAGTAGAAGCTGAAGAAAAGGAAGTTTTTCTTTCCGCTACCGATCTTGAAATTTCCATTA
AAACATCGCTATCCGCAGAAGTAATCCAAGCCGGAAGTATTTCTTTACCCGCTAAACAACTTTCCAGTTTTTTTAAAACG
ATCCATTTTGAGGAAACCATTTTGTCTTTAGAAGAATCCGACGGAGACTCTTCCATCGTTTATATCACGGACGCTTCCGG
CAAAAATGACTATAAGTCCAAAATCAGCGGAATGGATGCCGAAGAAATTAAAACGATTTCTAAAGTGAGTCCCTCTCAAA
TATCCTCTTTTCCAAGTAATTTGATAAACGACATGATCCGTAAAACTTCCTATGCGATCGCGCATGAAGATCAAAGGTTT
ATTTTTAACGGTCTTTATATGATTCCCGACGGAAATAAGTTGACTTTTGTCGGAACGGACGGGAGAAGACTTTGCAAGAT
TGAAAGAACTCTTCCTTCTCCTTTACAATTTAAGGATTCCATAATTGTTCCTGCAAAGGCGGTCCGAGAAATCTCTAAGA
TGATCGCAACTTCGGAAACCGGTAATCTCGGTTTGATCGATAGCCAAATTTATGCGTCTGCAAATAACATAGAACTTTTA
TGTAAATTGATCGAAGGAAATTTCCCAAACTACGAACAAGTCATTCCGAAAAGTACTAAATTTTCCATAACAATCTCAAA
GGAAGAATTCCAAGTATCTCTTAGACAAGTTTTAACTGCGGCGGAAGAACCTTCCAGACAAGTAAAACTCACATTCAGCA
AAAACAATTTGAATTTATTCGCTCAAACTCTTGGTGCTTCTGAAGCAAATATCAATAGGCCGATCGAATATTCCGGAAAT
GAAATTGCAATCGCCTTTAAAGGAGAATATCTCATGGATATTTTTAGGTCAATCGACGACAACGAAATTAAGATCGAATT
TTCCGATTCAAGTTCTCCGGTTATTTTCAAGGATCCGTCCGATCCTGAATTTATCTCCGTGATTATGCCGATGAAACTGT
AA

Upstream 100 bases:

>100_bases
TTCTTTAGGAATACAATTTTATGTACATATCTACAGGTACTACTTCTACAACTGTATAATAATAAAAATTTATTTAACTA
ATAAAAACTAGGAGAAAAAA

Downstream 100 bases:

>100_bases
TGTTCTTAAAACATCTTACTCTTCAAAATTTTAGAAGTTATGAAGAACTGAGCTTGGATTTCAATTCCAGGCTTATTTTT
TTTGTAGGCGATAACGGGGA

Product: DNA-directed DNA polymerase, beta subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MKIKVNTSDFLKAIHAVEGVISAREIKSILSNLKVEAEEKEVFLSATDLEISIKTSLSAEVIQAGSISLPAKQLSSFFKT
IHFEETILSLEESDGDSSIVYITDASGKNDYKSKISGMDAEEIKTISKVSPSQISSFPSNLINDMIRKTSYAIAHEDQRF
IFNGLYMIPDGNKLTFVGTDGRRLCKIERTLPSPLQFKDSIIVPAKAVREISKMIATSETGNLGLIDSQIYASANNIELL
CKLIEGNFPNYEQVIPKSTKFSITISKEEFQVSLRQVLTAAEEPSRQVKLTFSKNNLNLFAQTLGASEANINRPIEYSGN
EIAIAFKGEYLMDIFRSIDDNEIKIEFSDSSSPVIFKDPSDPEFISVIMPMKL

Sequences:

>Translated_373_residues
MKIKVNTSDFLKAIHAVEGVISAREIKSILSNLKVEAEEKEVFLSATDLEISIKTSLSAEVIQAGSISLPAKQLSSFFKT
IHFEETILSLEESDGDSSIVYITDASGKNDYKSKISGMDAEEIKTISKVSPSQISSFPSNLINDMIRKTSYAIAHEDQRF
IFNGLYMIPDGNKLTFVGTDGRRLCKIERTLPSPLQFKDSIIVPAKAVREISKMIATSETGNLGLIDSQIYASANNIELL
CKLIEGNFPNYEQVIPKSTKFSITISKEEFQVSLRQVLTAAEEPSRQVKLTFSKNNLNLFAQTLGASEANINRPIEYSGN
EIAIAFKGEYLMDIFRSIDDNEIKIEFSDSSSPVIFKDPSDPEFISVIMPMKL
>Mature_373_residues
MKIKVNTSDFLKAIHAVEGVISAREIKSILSNLKVEAEEKEVFLSATDLEISIKTSLSAEVIQAGSISLPAKQLSSFFKT
IHFEETILSLEESDGDSSIVYITDASGKNDYKSKISGMDAEEIKTISKVSPSQISSFPSNLINDMIRKTSYAIAHEDQRF
IFNGLYMIPDGNKLTFVGTDGRRLCKIERTLPSPLQFKDSIIVPAKAVREISKMIATSETGNLGLIDSQIYASANNIELL
CKLIEGNFPNYEQVIPKSTKFSITISKEEFQVSLRQVLTAAEEPSRQVKLTFSKNNLNLFAQTLGASEANINRPIEYSGN
EIAIAFKGEYLMDIFRSIDDNEIKIEFSDSSSPVIFKDPSDPEFISVIMPMKL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=380, Percent_Identity=25.7894736842105, Blast_Score=132, Evalue=5e-32,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41384; Mature: 41384

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKVNTSDFLKAIHAVEGVISAREIKSILSNLKVEAEEKEVFLSATDLEISIKTSLSAE
CEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCEEEEEEECEEEEEECCCCHH
VIQAGSISLPAKQLSSFFKTIHFEETILSLEESDGDSSIVYITDASGKNDYKSKISGMDA
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHCCCCH
EEIKTISKVSPSQISSFPSNLINDMIRKTSYAIAHEDQRFIFNGLYMIPDGNKLTFVGTD
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEECEEEEECCCEEEEEECC
GRRLCKIERTLPSPLQFKDSIIVPAKAVREISKMIATSETGNLGLIDSQIYASANNIELL
CCEEEEEECCCCCCCCCCCCEECCHHHHHHHHHHHHCCCCCCEEEEEHHHHCCCCCHHHH
CKLIEGNFPNYEQVIPKSTKFSITISKEEFQVSLRQVLTAAEEPSRQVKLTFSKNNLNLF
HHHHHCCCCCHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEE
AQTLGASEANINRPIEYSGNEIAIAFKGEYLMDIFRSIDDNEIKIEFSDSSSPVIFKDPS
EEHHCCCCCCCCCCEEECCCEEEEEECCHHHHHHHHCCCCCEEEEEECCCCCCEEEECCC
DPEFISVIMPMKL
CCCEEEEEECCCC
>Mature Secondary Structure
MKIKVNTSDFLKAIHAVEGVISAREIKSILSNLKVEAEEKEVFLSATDLEISIKTSLSAE
CEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCEEEEEEECEEEEEECCCCHH
VIQAGSISLPAKQLSSFFKTIHFEETILSLEESDGDSSIVYITDASGKNDYKSKISGMDA
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHCCCCH
EEIKTISKVSPSQISSFPSNLINDMIRKTSYAIAHEDQRFIFNGLYMIPDGNKLTFVGTD
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEECEEEEECCCEEEEEECC
GRRLCKIERTLPSPLQFKDSIIVPAKAVREISKMIATSETGNLGLIDSQIYASANNIELL
CCEEEEEECCCCCCCCCCCCEECCHHHHHHHHHHHHCCCCCCEEEEEHHHHCCCCCHHHH
CKLIEGNFPNYEQVIPKSTKFSITISKEEFQVSLRQVLTAAEEPSRQVKLTFSKNNLNLF
HHHHHCCCCCHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEE
AQTLGASEANINRPIEYSGNEIAIAFKGEYLMDIFRSIDDNEIKIEFSDSSSPVIFKDPS
EEHHCCCCCCCCCCEEECCCEEEEEECCHHHHHHHHCCCCCEEEEEECCCCCCEEEECCC
DPEFISVIMPMKL
CCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]