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The map label for this gene is gltD

Identifier: 162389967

GI number: 162389967

Start: 366933

End: 368399

Strand: Direct

Name: gltD

Synonym: Bamb_0322

Alternate gene names: 162389967

Gene position: 366933-368399 (Clockwise)

Preceding gene: 115350376

Following gene: 115350380

Centisome position: 10.32

GC content: 66.12

Gene sequence:

>1467_bases
ATGGGCAAGGCAACCGGTTTTCTGGAGTTCGAACGCCGCCACGAGGCGTACGAAGCACCGCTCACGCGCGTGAAGCACTA
CAAGGAATTCGTCGCGGCGCTGGCCGACGCGGACGCGAAGGTCCAGGGCGCGCGCTGTATGGATTGCGGCATCCCGTTCT
GCAACAACGGCTGCCCGGTCAACAACATCATCCCGGACTTCAACGATCTCGTTTACCGCCAGGACTGGCAGCAGGCGATC
GAAGTCCTGCATTCGACCAACAACTTCCCCGAGTTCACGGGCCGCATCTGCCCGGCGCCGTGCGAGGCGGCGTGCACGCT
CGGGATCAACGACGATCCGGTCGGCATCAAGTCGATCGAGCACGCGATCATCGACAAGGCCTGGGCGGAAGGCTGGGTGA
AGCCGCTGCCGCCCGAGCACAAGACCGGCAAGACGGTCGCGGTCGTCGGTTCGGGCCCCGCGGGCCTCGCCGTCGCGCAG
CAGCTCGCGCGTGCGGGCCACGACGTGACGGTGTTCGAGAAGAACGATCGCATCGGCGGCCTGCTGCGTTACGGGATCCC
CGACTTCAAGCTCGAGAAGTGGCTGATCGATCGCCGCATGCGCCAGATGGAAGCGGAAGGCGTGACGTTCCGCACCAGCG
TGTTCATCGGCAAGGATCCGCTGCCCGAGTCGATCGGCAGCCTCGCGAAGGAAACCATTTCGCCGGAGCAGCTGAAGGAA
GAATTCGACGCGGTCGTGATCGCGGGCGGTTCGGAGACGCCGCGCGACCTGCCGGTGCCGGGCCGCGAGCTCGCCGGCGT
CCATTTCGCGATGGACTTCCTGCCGCAGCAGAACCGCGTGAACGCGGGCGACAAGCTCGTCGACCAGCTGCTCGCGAAGG
GCAAGCACGTGATCGTGATCGGCGGCGGCGATACGGGTTCGGACTGCGTCGGCACGTCGAACCGTCACGGCGCGAAGCAG
GTCACGCAGTTCGAGCTGCTGCCGCAGCCGCCGGAAGAGGAAAACAAGCCGCTCGTGTGGCCGTACTGGCCGATCAAGCT
GCGCACGTCGTCGTCGCACGAAGAAGGTTGCGAGCGTGACTGGGCGGTCGCGACGAAGCGTCTCGAAGGCAAGAACGGCA
AGGTCGAGAAGCTGATCGCGGTGCGCGTCGAGTGGAAGGACGGCAAGATGCAGGAAGTGCCGGGCTCCGAATTCGAGATG
AAGGCCGACCTGGTGCTGCTCGCGATGGGCTTCACGCAGCCGGCAGCCACCGTGCTCGAGGCGTTCGGCGTCGCGAAGGA
TGCGCGCGGCAACGCGCGTGCGGCAACCGAAGGCGATCGCTCGTACTACACGTCGGTCGACAAGGTGTTCGCGGCCGGCG
ACATGCGTCGCGGTCAGTCGCTCGTCGTGTGGGCGATCCGCGAAGGCCGCCAGTGCGCGCGCTCGGTCGACGCGTATCTG
ATGGGGCATTCGAACCTGCCGCGATAA

Upstream 100 bases:

>100_bases
GTGCTGGCCGCCTGAGCGACGAACGACATAGCGAATGCCGCGCGCGCATGACTGCCGCGCGTGGCTCCCCCACCCGATAC
ACCGAACAGAAGAGCACCTT

Downstream 100 bases:

>100_bases
GCGCGGTTCGCGGGCCACTGCCGTAGCAGCCGGGCGTCCGAAAGGGCGCCCGGTTTTTTTATGGAACACCGGTTACAGGA
GCGGCCGCTCGGAGCAATTG

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MGKATGFLEFERRHEAYEAPLTRVKHYKEFVAALADADAKVQGARCMDCGIPFCNNGCPVNNIIPDFNDLVYRQDWQQAI
EVLHSTNNFPEFTGRICPAPCEAACTLGINDDPVGIKSIEHAIIDKAWAEGWVKPLPPEHKTGKTVAVVGSGPAGLAVAQ
QLARAGHDVTVFEKNDRIGGLLRYGIPDFKLEKWLIDRRMRQMEAEGVTFRTSVFIGKDPLPESIGSLAKETISPEQLKE
EFDAVVIAGGSETPRDLPVPGRELAGVHFAMDFLPQQNRVNAGDKLVDQLLAKGKHVIVIGGGDTGSDCVGTSNRHGAKQ
VTQFELLPQPPEEENKPLVWPYWPIKLRTSSSHEEGCERDWAVATKRLEGKNGKVEKLIAVRVEWKDGKMQEVPGSEFEM
KADLVLLAMGFTQPAATVLEAFGVAKDARGNARAATEGDRSYYTSVDKVFAAGDMRRGQSLVVWAIREGRQCARSVDAYL
MGHSNLPR

Sequences:

>Translated_488_residues
MGKATGFLEFERRHEAYEAPLTRVKHYKEFVAALADADAKVQGARCMDCGIPFCNNGCPVNNIIPDFNDLVYRQDWQQAI
EVLHSTNNFPEFTGRICPAPCEAACTLGINDDPVGIKSIEHAIIDKAWAEGWVKPLPPEHKTGKTVAVVGSGPAGLAVAQ
QLARAGHDVTVFEKNDRIGGLLRYGIPDFKLEKWLIDRRMRQMEAEGVTFRTSVFIGKDPLPESIGSLAKETISPEQLKE
EFDAVVIAGGSETPRDLPVPGRELAGVHFAMDFLPQQNRVNAGDKLVDQLLAKGKHVIVIGGGDTGSDCVGTSNRHGAKQ
VTQFELLPQPPEEENKPLVWPYWPIKLRTSSSHEEGCERDWAVATKRLEGKNGKVEKLIAVRVEWKDGKMQEVPGSEFEM
KADLVLLAMGFTQPAATVLEAFGVAKDARGNARAATEGDRSYYTSVDKVFAAGDMRRGQSLVVWAIREGRQCARSVDAYL
MGHSNLPR
>Mature_487_residues
GKATGFLEFERRHEAYEAPLTRVKHYKEFVAALADADAKVQGARCMDCGIPFCNNGCPVNNIIPDFNDLVYRQDWQQAIE
VLHSTNNFPEFTGRICPAPCEAACTLGINDDPVGIKSIEHAIIDKAWAEGWVKPLPPEHKTGKTVAVVGSGPAGLAVAQQ
LARAGHDVTVFEKNDRIGGLLRYGIPDFKLEKWLIDRRMRQMEAEGVTFRTSVFIGKDPLPESIGSLAKETISPEQLKEE
FDAVVIAGGSETPRDLPVPGRELAGVHFAMDFLPQQNRVNAGDKLVDQLLAKGKHVIVIGGGDTGSDCVGTSNRHGAKQV
TQFELLPQPPEEENKPLVWPYWPIKLRTSSSHEEGCERDWAVATKRLEGKNGKVEKLIAVRVEWKDGKMQEVPGSEFEMK
ADLVLLAMGFTQPAATVLEAFGVAKDARGNARAATEGDRSYYTSVDKVFAAGDMRRGQSLVVWAIREGRQCARSVDAYLM
GHSNLPR

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=493, Percent_Identity=26.5720081135903, Blast_Score=114, Evalue=3e-25,
Organism=Escherichia coli, GI1789606, Length=486, Percent_Identity=34.156378600823, Blast_Score=242, Evalue=3e-65,
Organism=Escherichia coli, GI1788811, Length=455, Percent_Identity=36.4835164835165, Blast_Score=239, Evalue=2e-64,
Organism=Escherichia coli, GI87082180, Length=470, Percent_Identity=33.8297872340426, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI1788468, Length=462, Percent_Identity=28.3549783549784, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI1789243, Length=444, Percent_Identity=27.9279279279279, Blast_Score=120, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17570289, Length=506, Percent_Identity=46.0474308300395, Blast_Score=432, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI71984108, Length=487, Percent_Identity=26.0780287474333, Blast_Score=99, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6320030, Length=502, Percent_Identity=50.199203187251, Blast_Score=480, Evalue=1e-136,
Organism=Drosophila melanogaster, GI28574881, Length=503, Percent_Identity=50.6958250497018, Blast_Score=482, Evalue=1e-136,
Organism=Drosophila melanogaster, GI24665539, Length=503, Percent_Identity=50.6958250497018, Blast_Score=482, Evalue=1e-136,
Organism=Drosophila melanogaster, GI24665547, Length=503, Percent_Identity=50.6958250497018, Blast_Score=481, Evalue=1e-136,
Organism=Drosophila melanogaster, GI24665543, Length=503, Percent_Identity=50.6958250497018, Blast_Score=481, Evalue=1e-136,
Organism=Drosophila melanogaster, GI24640763, Length=487, Percent_Identity=24.6406570841889, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI18858217, Length=487, Percent_Identity=24.6406570841889, Blast_Score=92, Evalue=1e-18,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 53615; Mature: 53483

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKATGFLEFERRHEAYEAPLTRVKHYKEFVAALADADAKVQGARCMDCGIPFCNNGCPV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCC
NNIIPDFNDLVYRQDWQQAIEVLHSTNNFPEFTGRICPAPCEAACTLGINDDPVGIKSIE
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCEEEECCCCCCCCHHHHH
HAIIDKAWAEGWVKPLPPEHKTGKTVAVVGSGPAGLAVAQQLARAGHDVTVFEKNDRIGG
HHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCCCC
LLRYGIPDFKLEKWLIDRRMRQMEAEGVTFRTSVFIGKDPLPESIGSLAKETISPEQLKE
EEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHCCCHHHHHH
EFDAVVIAGGSETPRDLPVPGRELAGVHFAMDFLPQQNRVNAGDKLVDQLLAKGKHVIVI
HCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCEEEEE
GGGDTGSDCVGTSNRHGAKQVTQFELLPQPPEEENKPLVWPYWPIKLRTSSSHEEGCERD
ECCCCCCCCCCCCCCCCHHHHHHEECCCCCCCCCCCCEEECCCCEEEECCCCCHHHCCHH
WAVATKRLEGKNGKVEKLIAVRVEWKDGKMQEVPGSEFEMKADLVLLAMGFTQPAATVLE
HHHHHHHHCCCCCCEEEEEEEEEEECCCCCCCCCCCCCEEECCEEEEEECCCCHHHHHHH
AFGVAKDARGNARAATEGDRSYYTSVDKVFAAGDMRRGQSLVVWAIREGRQCARSVDAYL
HHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHHH
MGHSNLPR
CCCCCCCC
>Mature Secondary Structure 
GKATGFLEFERRHEAYEAPLTRVKHYKEFVAALADADAKVQGARCMDCGIPFCNNGCPV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCC
NNIIPDFNDLVYRQDWQQAIEVLHSTNNFPEFTGRICPAPCEAACTLGINDDPVGIKSIE
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCEEEECCCCCCCCHHHHH
HAIIDKAWAEGWVKPLPPEHKTGKTVAVVGSGPAGLAVAQQLARAGHDVTVFEKNDRIGG
HHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCCCC
LLRYGIPDFKLEKWLIDRRMRQMEAEGVTFRTSVFIGKDPLPESIGSLAKETISPEQLKE
EEECCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHCCCHHHHHH
EFDAVVIAGGSETPRDLPVPGRELAGVHFAMDFLPQQNRVNAGDKLVDQLLAKGKHVIVI
HCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCEEEEE
GGGDTGSDCVGTSNRHGAKQVTQFELLPQPPEEENKPLVWPYWPIKLRTSSSHEEGCERD
ECCCCCCCCCCCCCCCCHHHHHHEECCCCCCCCCCCCEEECCCCEEEECCCCCHHHCCHH
WAVATKRLEGKNGKVEKLIAVRVEWKDGKMQEVPGSEFEMKADLVLLAMGFTQPAATVLE
HHHHHHHHCCCCCCEEEEEEEEEEECCCCCCCCCCCCCEEECCEEEEEECCCCHHHHHHH
AFGVAKDARGNARAATEGDRSYYTSVDKVFAAGDMRRGQSLVVWAIREGRQCARSVDAYL
HHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHHH
MGHSNLPR
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]