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The map label for this gene is dapF

Identifier: 115353182

GI number: 115353182

Start: 3449663

End: 3450532

Strand: Direct

Name: dapF

Synonym: Bamb_3131

Alternate gene names: 115353182

Gene position: 3449663-3450532 (Clockwise)

Preceding gene: 115353181

Following gene: 115353183

Centisome position: 96.99

GC content: 69.08

Gene sequence:

>870_bases
GTGAAACTCTCGTTCACCAAGATGCACGGCGCGGGCAACGACTTCGTCGTGCTCGACGGCTATTCGCGTGCGTTGCCGCC
GCTCACCGAAGCGCAGGTGCGCGCGCTCGCCAACCGGCACTTCGGCATCGGCGCCGACCAGTTGCTGCTCGTCGAAAAGC
CGACCGTCGACGGCGCGGATTTCAAGTACCGGATCTTCAATTGCGACGGCGGCGAGGTCGAACACTGCGGCAACGGCGCA
CGCTGCTTCGTGAAGTTCGTCAGCGACCGCGGCCTGACCGACAAGCGCAGCGTGCGCGTGCAGGTCATGAAGGGCCTGAT
CACGCTGACGCTGCAGGACAACGGCGAAGTCGTCGTCGACATGGGCGCGCCCGTATTCGCGCCGGCGCAGGTGCCGTTCG
ATGTATCGGGCCTCGACGGCCGCGCCGAAGGCCGCGACACGCTGTGGCCGCTGGACGTCGGCGGCGCGACGCGCTGGATT
TCGGCGGTGTCGATGGGCAATCCGCATGCGGTGCAGGTCGTCGACGACGTCGAGGCGTATCCGGTGCTGGAAGAAGGCCC
GCTGATCGAGCGCCATGCGCGCTTCCCGCAGCGCGTGAACGCCGGCTTCATGCAGATCGTGTCGCGCCACGAAGTGAAGC
TGCGCGTGTACGAACGCGGCGCGGGCGAGACGCTCGCGTGCGGCACGGGCGCGTGCGCGGCGGTCGCGGCCGGCATCCGG
CGCGGCCTGCTCGATTCGCCGGTGACCGTCCATACGCACGGCGGCACGCTGACGATCGGCTGGGACGGCGCGCGCGACGA
AGCTGCCGCACTGATGATGGCCGGGCCCGCCGCGACCGTGTTCGAAGGCGAGATCGACCTCACCGCCTGA

Upstream 100 bases:

>100_bases
CATTTGCGGCTGAGATCAGCACGACGAACGACGCCGCCCGGCACGGCCGCCGGCCTCGCGTCGTGCTGGCCGCCTTGTTC
GTCATGGAATTCCGGACCCA

Downstream 100 bases:

>100_bases
CCCTGCAACGTCCTTGATAACTGAACGCTCCAGCCACATGAACGATCGCGAAGTCGCCGACTACCTGCTCGCCAATCCCG
AATTCTTCGCGCAGCACGCC

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MKLSFTKMHGAGNDFVVLDGYSRALPPLTEAQVRALANRHFGIGADQLLLVEKPTVDGADFKYRIFNCDGGEVEHCGNGA
RCFVKFVSDRGLTDKRSVRVQVMKGLITLTLQDNGEVVVDMGAPVFAPAQVPFDVSGLDGRAEGRDTLWPLDVGGATRWI
SAVSMGNPHAVQVVDDVEAYPVLEEGPLIERHARFPQRVNAGFMQIVSRHEVKLRVYERGAGETLACGTGACAAVAAGIR
RGLLDSPVTVHTHGGTLTIGWDGARDEAAALMMAGPAATVFEGEIDLTA

Sequences:

>Translated_289_residues
MKLSFTKMHGAGNDFVVLDGYSRALPPLTEAQVRALANRHFGIGADQLLLVEKPTVDGADFKYRIFNCDGGEVEHCGNGA
RCFVKFVSDRGLTDKRSVRVQVMKGLITLTLQDNGEVVVDMGAPVFAPAQVPFDVSGLDGRAEGRDTLWPLDVGGATRWI
SAVSMGNPHAVQVVDDVEAYPVLEEGPLIERHARFPQRVNAGFMQIVSRHEVKLRVYERGAGETLACGTGACAAVAAGIR
RGLLDSPVTVHTHGGTLTIGWDGARDEAAALMMAGPAATVFEGEIDLTA
>Mature_289_residues
MKLSFTKMHGAGNDFVVLDGYSRALPPLTEAQVRALANRHFGIGADQLLLVEKPTVDGADFKYRIFNCDGGEVEHCGNGA
RCFVKFVSDRGLTDKRSVRVQVMKGLITLTLQDNGEVVVDMGAPVFAPAQVPFDVSGLDGRAEGRDTLWPLDVGGATRWI
SAVSMGNPHAVQVVDDVEAYPVLEEGPLIERHARFPQRVNAGFMQIVSRHEVKLRVYERGAGETLACGTGACAAVAAGIR
RGLLDSPVTVHTHGGTLTIGWDGARDEAAALMMAGPAATVFEGEIDLTA

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=285, Percent_Identity=49.4736842105263, Blast_Score=267, Evalue=7e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_BURCM (Q0BAY6)

Other databases:

- EMBL:   CP000440
- RefSeq:   YP_775021.1
- ProteinModelPortal:   Q0BAY6
- SMR:   Q0BAY6
- STRING:   Q0BAY6
- GeneID:   4310613
- GenomeReviews:   CP000440_GR
- KEGG:   bam:Bamb_3131
- NMPDR:   fig|339670.3.peg.35
- eggNOG:   COG0253
- HOGENOM:   HBG399442
- OMA:   HTGIGFD
- ProtClustDB:   PRK00450
- BioCyc:   BAMB339670:BAMB_3131-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 30855; Mature: 30855

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 76-76 ACT_SITE 227-227

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSFTKMHGAGNDFVVLDGYSRALPPLTEAQVRALANRHFGIGADQLLLVEKPTVDGAD
CCEEEEEECCCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCC
FKYRIFNCDGGEVEHCGNGARCFVKFVSDRGLTDKRSVRVQVMKGLITLTLQDNGEVVVD
EEEEEEECCCCCHHHCCCCCEEEEEEECCCCCCCHHHHHHEEEECEEEEEECCCCCEEEE
MGAPVFAPAQVPFDVSGLDGRAEGRDTLWPLDVGGATRWISAVSMGNPHAVQVVDDVEAY
CCCCEEECCCCCEEECCCCCCCCCCCCEEEEECCCHHHHHHHHCCCCCEEEEEEECCCCC
PVLEEGPLIERHARFPQRVNAGFMQIVSRHEVKLRVYERGAGETLACGTGACAAVAAGIR
CCCCCCCCHHHHHCCCHHHCHHHHHHHHCCCEEEEEEECCCCCEEECCCCHHHHHHHHHH
RGLLDSPVTVHTHGGTLTIGWDGARDEAAALMMAGPAATVFEGEIDLTA
HCCCCCCEEEEECCCEEEEECCCCCCCCEEEEEECCCCEEEECCEEECC
>Mature Secondary Structure
MKLSFTKMHGAGNDFVVLDGYSRALPPLTEAQVRALANRHFGIGADQLLLVEKPTVDGAD
CCEEEEEECCCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCC
FKYRIFNCDGGEVEHCGNGARCFVKFVSDRGLTDKRSVRVQVMKGLITLTLQDNGEVVVD
EEEEEEECCCCCHHHCCCCCEEEEEEECCCCCCCHHHHHHEEEECEEEEEECCCCCEEEE
MGAPVFAPAQVPFDVSGLDGRAEGRDTLWPLDVGGATRWISAVSMGNPHAVQVVDDVEAY
CCCCEEECCCCCEEECCCCCCCCCCCCEEEEECCCHHHHHHHHCCCCCEEEEEEECCCCC
PVLEEGPLIERHARFPQRVNAGFMQIVSRHEVKLRVYERGAGETLACGTGACAAVAAGIR
CCCCCCCCHHHHHCCCHHHCHHHHHHHHCCCEEEEEEECCCCCEEECCCCHHHHHHHHHH
RGLLDSPVTVHTHGGTLTIGWDGARDEAAALMMAGPAATVFEGEIDLTA
HCCCCCCEEEEECCCEEEEECCCCCCCCEEEEEECCCCEEEECCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA