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The map label for this gene is htrB [H]

Identifier: 115353181

GI number: 115353181

Start: 3448548

End: 3449432

Strand: Direct

Name: htrB [H]

Synonym: Bamb_3130

Alternate gene names: 115353181

Gene position: 3448548-3449432 (Clockwise)

Preceding gene: 115353179

Following gene: 115353182

Centisome position: 96.96

GC content: 65.88

Gene sequence:

>885_bases
ATGCTAGGCCGCCTCGGCACGCATCTCGCCATCGGCTTTCTGAAGCTGCTCGCCCTGCTGCCGTACGGTTTGACCGCGCG
GTTCGGCGATGGCCTCGGCTGGCTGCTGTACCAGATTCCCAGCCGGCGAAAGCGCATCGTACATACCAATCTGAAACTCT
GCTTCCCCGAGTGGAGCGACGAACGGCGCGAGGAAGTGGCCGGCCAGCATTTCCGCCATGCGATCCGCAGCTACGTCGAG
CGCAGCTATCAGTGGTTCGCGTCGGAACAGGCCTACCGCAAGCTGTTCACGATCGACAGCGAGATCGACCTGAGCGATCC
GGACATGCCGCCCACGCTCCTGCTCGGTCTCCATTTCGTCGGCATCGAGGCCGGCTCGATGGCGCTCAATCTCGCACTCG
GCCGTATGTGCGGCTCGCTCTATACGCCGATGAAGAACCCGGAAATCGACGCCGCGGCCAAAGCGGGCCGCAGCCGCTTC
GGCGCGGAACTGGCGGGCCGCGCGGACAGCGCGCGCATCGTGCTGCGCTGGCTGCGCGACCGCAAGCCCGTGATGCTCGG
CGCGGACATGGACTACGGGCTGCGCAACTCCACGTTCGTGCCCTTCTTCGGCGTACCGGCCTGCACGCTGACGGCCGTCG
GCCGCCTCGCCAAGACCGGCCGCGCGCAGGTCGTGCCGTTCATCGGCGAGGTGCTGCCGAACTACAAGGGCTACCGGCTG
AAGGTATTCAAGCCGTGGGATCACTACCCGACCGGCGACGACGATCTCGACGCGCGGCGGATGAACGAATTCCTCGAGGA
ACAGATCCCGCTGATGCCCGAGCAGTACTACTGGGTTCACAAGCGCTTCAAGACGCGCCCGCCCGGCGAGCCGAGCCTGT
ACTGA

Upstream 100 bases:

>100_bases
CTTTCTTGAATTGTCACAGAGCGCCGCCCGTGCTGTATCGCCCGCTGTCCTACCCCCCTGTTCGCAACGTGCCGGCCAGC
CGGCCCCACGGAGATTCCGC

Downstream 100 bases:

>100_bases
CCCCGCCGGGCGACCGCGAGCGTGCCGTCATGCGCTCGCCGGCCTCCCCAAGCCCCGCTTCGCGACGCAGCGCCATTTCC
GGCGCGATGCCGGCATGGCC

Product: lipid A biosynthesis lauroyl acyltransferase

Products: KDO2-(lauroyl)-lipid IVA [C]

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MLGRLGTHLAIGFLKLLALLPYGLTARFGDGLGWLLYQIPSRRKRIVHTNLKLCFPEWSDERREEVAGQHFRHAIRSYVE
RSYQWFASEQAYRKLFTIDSEIDLSDPDMPPTLLLGLHFVGIEAGSMALNLALGRMCGSLYTPMKNPEIDAAAKAGRSRF
GAELAGRADSARIVLRWLRDRKPVMLGADMDYGLRNSTFVPFFGVPACTLTAVGRLAKTGRAQVVPFIGEVLPNYKGYRL
KVFKPWDHYPTGDDDLDARRMNEFLEEQIPLMPEQYYWVHKRFKTRPPGEPSLY

Sequences:

>Translated_294_residues
MLGRLGTHLAIGFLKLLALLPYGLTARFGDGLGWLLYQIPSRRKRIVHTNLKLCFPEWSDERREEVAGQHFRHAIRSYVE
RSYQWFASEQAYRKLFTIDSEIDLSDPDMPPTLLLGLHFVGIEAGSMALNLALGRMCGSLYTPMKNPEIDAAAKAGRSRF
GAELAGRADSARIVLRWLRDRKPVMLGADMDYGLRNSTFVPFFGVPACTLTAVGRLAKTGRAQVVPFIGEVLPNYKGYRL
KVFKPWDHYPTGDDDLDARRMNEFLEEQIPLMPEQYYWVHKRFKTRPPGEPSLY
>Mature_294_residues
MLGRLGTHLAIGFLKLLALLPYGLTARFGDGLGWLLYQIPSRRKRIVHTNLKLCFPEWSDERREEVAGQHFRHAIRSYVE
RSYQWFASEQAYRKLFTIDSEIDLSDPDMPPTLLLGLHFVGIEAGSMALNLALGRMCGSLYTPMKNPEIDAAAKAGRSRF
GAELAGRADSARIVLRWLRDRKPVMLGADMDYGLRNSTFVPFFGVPACTLTAVGRLAKTGRAQVVPFIGEVLPNYKGYRL
KVFKPWDHYPTGDDDLDARRMNEFLEEQIPLMPEQYYWVHKRFKTRPPGEPSLY

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=291, Percent_Identity=35.7388316151203, Blast_Score=160, Evalue=6e-41,
Organism=Escherichia coli, GI87082094, Length=291, Percent_Identity=32.6460481099656, Blast_Score=151, Evalue=6e-38,
Organism=Escherichia coli, GI1788161, Length=305, Percent_Identity=31.4754098360656, Blast_Score=104, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.- [C]

Molecular weight: Translated: 33526; Mature: 33526

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGRLGTHLAIGFLKLLALLPYGLTARFGDGLGWLLYQIPSRRKRIVHTNLKLCFPEWSD
CCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHCCHHHHHEEECCCEEECCCCCH
ERREEVAGQHFRHAIRSYVERSYQWFASEQAYRKLFTIDSEIDLSDPDMPPTLLLGLHFV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
GIEAGSMALNLALGRMCGSLYTPMKNPEIDAAAKAGRSRFGAELAGRADSARIVLRWLRD
EECCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCHHHHCCCCHHHHHHHHHHC
RKPVMLGADMDYGLRNSTFVPFFGVPACTLTAVGRLAKTGRAQVVPFIGEVLPNYKGYRL
CCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCEE
KVFKPWDHYPTGDDDLDARRMNEFLEEQIPLMPEQYYWVHKRFKTRPPGEPSLY
EEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MLGRLGTHLAIGFLKLLALLPYGLTARFGDGLGWLLYQIPSRRKRIVHTNLKLCFPEWSD
CCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHCCHHHHHEEECCCEEECCCCCH
ERREEVAGQHFRHAIRSYVERSYQWFASEQAYRKLFTIDSEIDLSDPDMPPTLLLGLHFV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
GIEAGSMALNLALGRMCGSLYTPMKNPEIDAAAKAGRSRFGAELAGRADSARIVLRWLRD
EECCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCHHHHCCCCHHHHHHHHHHC
RKPVMLGADMDYGLRNSTFVPFFGVPACTLTAVGRLAKTGRAQVVPFIGEVLPNYKGYRL
CCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCEE
KVFKPWDHYPTGDDDLDARRMNEFLEEQIPLMPEQYYWVHKRFKTRPPGEPSLY
EEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: KDO2-lipid IVA; lauroyl-ACP [C]

Specific reaction: KDO2-lipid IVA + lauroyl-ACP = KDO2-(lauroyl)-lipid IVA + [acyl carrier protein] [C]

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]