Click here to switch to the map view.

The map label for this gene is gacA [H]

Identifier: 115353151

GI number: 115353151

Start: 3421399

End: 3422055

Strand: Direct

Name: gacA [H]

Synonym: Bamb_3100

Alternate gene names: 115353151

Gene position: 3421399-3422055 (Clockwise)

Preceding gene: 115353150

Following gene: 115353152

Centisome position: 96.2

GC content: 65.14

Gene sequence:

>657_bases
ATGAGCCTGGACATCCTGCTCGTGGACGACCACGCAATCGTCCGGCAAGGGGTCCGCCACTTGCTGATCGACCGCGGCGT
CGCGCGCGAAGTGACCGAAGCCGAGACCGGCAGCGATGCGGTGGCCGCCGTCGACCGCCAGACCTTCGACGTGATCCTGC
TCGATATCTCGCTGCCGGACACCAACGGCATCGAGTTGCTCAAGCGCATCAAGCGCAAGCTGCCGGGCACGCCGGTGCTG
ATGTTCTCGATGTACCGCGAGGACCAGTACGCGGTGCGCGCGCTGAAGGCCGGCGCGTCCGGCTATCTGTCGAAGACGGT
GAATGCCGCGCAGATGATCGGCGCGATCCAGCAGGTCGCGGCCGGCCGCAAGTACGTGAGCCCGGCGATGGCCGAGGCGC
TCGCCGAATACGTGTCGTTCGAGAACGAACCGCTGCCGCACGAGAAGCTGTCCGATCGCGAATACCAGACGCTCTGCATG
CTGGCATCGGGCAAGCGCCTCACCGATATCGCGAACACGCTATCGCTGTCGGTGAAGACGGTCAGCGTGTACCGGTCGCG
GCTGCTCGAGAAGATGCGGCTGTCGAACAACGCGGAACTGACGTTCTACGTGATGAGCAACCGGCTCGTCGACATGGCGC
CTGCATCGGGCGCCTGA

Upstream 100 bases:

>100_bases
GAATCGGGCCGCGGCACGCGCATCACCGCGCGTTTCGCATGGAACTCGCTGTTCGCGGTGCCGGCCGCCACGCGCCGCGC
GTCGCTTTCGTGAGGTCGCG

Downstream 100 bases:

>100_bases
TTCATCGGATTCACCGGAATCGCGGGAGGCGCGCCGGGCCGCGCCGGCCTGCCGCTTCAGCCCCGGTCGCCGCACGCTGC
GCGCAAGCGCTTGTTTTCTC

Product: two component LuxR family transcriptional regulator

Products: NA

Alternate protein names: Global activator [H]

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MSLDILLVDDHAIVRQGVRHLLIDRGVAREVTEAETGSDAVAAVDRQTFDVILLDISLPDTNGIELLKRIKRKLPGTPVL
MFSMYREDQYAVRALKAGASGYLSKTVNAAQMIGAIQQVAAGRKYVSPAMAEALAEYVSFENEPLPHEKLSDREYQTLCM
LASGKRLTDIANTLSLSVKTVSVYRSRLLEKMRLSNNAELTFYVMSNRLVDMAPASGA

Sequences:

>Translated_218_residues
MSLDILLVDDHAIVRQGVRHLLIDRGVAREVTEAETGSDAVAAVDRQTFDVILLDISLPDTNGIELLKRIKRKLPGTPVL
MFSMYREDQYAVRALKAGASGYLSKTVNAAQMIGAIQQVAAGRKYVSPAMAEALAEYVSFENEPLPHEKLSDREYQTLCM
LASGKRLTDIANTLSLSVKTVSVYRSRLLEKMRLSNNAELTFYVMSNRLVDMAPASGA
>Mature_217_residues
SLDILLVDDHAIVRQGVRHLLIDRGVAREVTEAETGSDAVAAVDRQTFDVILLDISLPDTNGIELLKRIKRKLPGTPVLM
FSMYREDQYAVRALKAGASGYLSKTVNAAQMIGAIQQVAAGRKYVSPAMAEALAEYVSFENEPLPHEKLSDREYQTLCML
ASGKRLTDIANTLSLSVKTVSVYRSRLLEKMRLSNNAELTFYVMSNRLVDMAPASGA

Specific function: Positively controls the production of the autoinducer N- butyryl-homoserine lactone and the formation of the virulence factors pyocyanine, cyanide, and lipase [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=210, Percent_Identity=33.3333333333333, Blast_Score=140, Evalue=7e-35,
Organism=Escherichia coli, GI1790102, Length=198, Percent_Identity=36.8686868686869, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1788521, Length=205, Percent_Identity=31.219512195122, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1788712, Length=209, Percent_Identity=27.2727272727273, Blast_Score=91, Evalue=5e-20,
Organism=Escherichia coli, GI1786747, Length=212, Percent_Identity=30.6603773584906, Blast_Score=86, Evalue=1e-18,
Organism=Escherichia coli, GI1787473, Length=210, Percent_Identity=31.4285714285714, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788546, Length=217, Percent_Identity=25.8064516129032, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1786599, Length=156, Percent_Identity=30.1282051282051, Blast_Score=67, Evalue=7e-13,
Organism=Escherichia coli, GI1790437, Length=110, Percent_Identity=37.2727272727273, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788191, Length=112, Percent_Identity=35.7142857142857, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 24006; Mature: 23875

Theoretical pI: Translated: 7.59; Mature: 7.59

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLDILLVDDHAIVRQGVRHLLIDRGVAREVTEAETGSDAVAAVDRQTFDVILLDISLPD
CCEEEEEECCHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHCCCEEEEEEEEEECCC
TNGIELLKRIKRKLPGTPVLMFSMYREDQYAVRALKAGASGYLSKTVNAAQMIGAIQQVA
CCHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
AGRKYVSPAMAEALAEYVSFENEPLPHEKLSDREYQTLCMLASGKRLTDIANTLSLSVKT
CCCHHCCHHHHHHHHHHHCCCCCCCCCHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHH
VSVYRSRLLEKMRLSNNAELTFYVMSNRLVDMAPASGA
HHHHHHHHHHHHHCCCCCCEEEEEECCCEEECCCCCCC
>Mature Secondary Structure 
SLDILLVDDHAIVRQGVRHLLIDRGVAREVTEAETGSDAVAAVDRQTFDVILLDISLPD
CEEEEEECCHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHCCCEEEEEEEEEECCC
TNGIELLKRIKRKLPGTPVLMFSMYREDQYAVRALKAGASGYLSKTVNAAQMIGAIQQVA
CCHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
AGRKYVSPAMAEALAEYVSFENEPLPHEKLSDREYQTLCMLASGKRLTDIANTLSLSVKT
CCCHHCCHHHHHHHHHHHCCCCCCCCCHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHH
VSVYRSRLLEKMRLSNNAELTFYVMSNRLVDMAPASGA
HHHHHHHHHHHHHCCCCCCEEEEEECCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9159518; 10984043 [H]