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The map label for this gene is puuA [H]

Identifier: 115353092

GI number: 115353092

Start: 3357356

End: 3358693

Strand: Direct

Name: puuA [H]

Synonym: Bamb_3041

Alternate gene names: 115353092

Gene position: 3357356-3358693 (Clockwise)

Preceding gene: 115353091

Following gene: 115353093

Centisome position: 94.4

GC content: 65.62

Gene sequence:

>1338_bases
ATGCAAGACATGGAAGACTTTCTGAGACAGCACCGGATCACCGAAGTCGAGGCGATCATCCCCGACATGGCCGGCATCGC
ACGCGGCAAGATCACGCCGCGCAACAAGTTCACGTCCGGCGAATCGATGCGCCTGCCGCAGGCCGTGATGGTGCAGACCG
TCACCGGCGAATATCCGGAAGACGGCTCGCTGACCGGCGTGACCGATCCCGACATGGTGTGCGTGCCCGACGCGTCGACG
ATCCGCCTGATCCCGTGGGCCAGCGACCCGACCGCGCAGGTGATCCACGACTGCGTGCACTTCGACGGCTCGCCGGTCGA
GATCTCGCCGCGCTACGTGCTGCGCCGCGTGCTCGACCTGTACAAGGCGAAGGGCTGGAAGCCGGTGGTCGCGCCGGAGC
TCGAGTTCTACCTGGTCGACATGAACAAGGATCCCGACCTGCCGCTGCGTCCGCCGGTCGGCCGCACGGGCCGCCCCGAA
ACGGGCCGCCAGTCGTATTCGATCGAGGCCGTCAACGAGTTCGATCCGCTGTTCGAGGACATCTACGAGTACTGCGAAAC
ACAGGGTCTCGACATCGACACGCTGATCCACGAAGTCGGCGCCGCGCAGATGGAGATCAACTTCATGCACGGCGACGCGC
TGTCGCTGGCCGACCAGGTGTTCCTGTTCAAGCGCACGGTGCGCGAGGCGGCGCTGCGCCACAACATGTACGCGACGTTC
ATGGCCAAGCCGATGGAAGGCGAGCCGGGCTCCGCGATGCACGTGCACCAGAGCATCGTCGACGAGGAAACGGGCCGCAA
CCTGTTCACGTCGCCCGAGACGGGCGGTGCGACCTCGCTGTTCTACAACTACCTCGCCGGGCTGCAGAAGTACACGCCGG
CGCTGATGCCGATCTTCGCGCCGTACATCAACTCGTATCGCCGCCTGTCGCGCTTCATGGCCGCGCCGATCAACGTGCAG
TGGGGCTACGACAACCGCACGGTCGGCTTCCGCATCCCGCACTCGGGCCCGGCCGCACGCCGCATCGAGAACCGGATCCC
GGGCGTCGACTGCAACCCGTACCTCGCGCTCGCGGCGACGCTCGCGGCCGGCTATCTCGGCATGACGCAGCGCCTCGAGC
CGACCGAGCCGCTCGTCAGCGATGGCTACGACCTGCCGTACCAGCTGCCGCGCAACCTCGAGGAAGGACTCACGCTGATG
GCCGCGTGCGAGCCGCTCGGCGAGATTCTCGGCCACAAGTTCCTGAAAGCGTATTTCGCGCTGAAGGAAACCGAATACGA
AGCGTTCTTCCGCGTGATCAGCTCGTGGGAACGCCGTCATCTGCTGCTGCACGTGTAA

Upstream 100 bases:

>100_bases
CGTTCGGCGACGCCTGCCGCGCCCGCCGCGCCGCCCGCACGCACACCACGGCCGCCGCCGCGCTCGCCTGAGCGCGCCGA
CCCACACAACGAGAACGGAC

Downstream 100 bases:

>100_bases
GCGCGCGCACGAACGTACCAACGAATACGGAGGAAACATGACGTACCGCAACGAATCAGCGTGGATCCAGCCGGCCGCAC
CGGCCGTCGCACCGCGCACG

Product: glutamate--ammonia ligase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MQDMEDFLRQHRITEVEAIIPDMAGIARGKITPRNKFTSGESMRLPQAVMVQTVTGEYPEDGSLTGVTDPDMVCVPDAST
IRLIPWASDPTAQVIHDCVHFDGSPVEISPRYVLRRVLDLYKAKGWKPVVAPELEFYLVDMNKDPDLPLRPPVGRTGRPE
TGRQSYSIEAVNEFDPLFEDIYEYCETQGLDIDTLIHEVGAAQMEINFMHGDALSLADQVFLFKRTVREAALRHNMYATF
MAKPMEGEPGSAMHVHQSIVDEETGRNLFTSPETGGATSLFYNYLAGLQKYTPALMPIFAPYINSYRRLSRFMAAPINVQ
WGYDNRTVGFRIPHSGPAARRIENRIPGVDCNPYLALAATLAAGYLGMTQRLEPTEPLVSDGYDLPYQLPRNLEEGLTLM
AACEPLGEILGHKFLKAYFALKETEYEAFFRVISSWERRHLLLHV

Sequences:

>Translated_445_residues
MQDMEDFLRQHRITEVEAIIPDMAGIARGKITPRNKFTSGESMRLPQAVMVQTVTGEYPEDGSLTGVTDPDMVCVPDAST
IRLIPWASDPTAQVIHDCVHFDGSPVEISPRYVLRRVLDLYKAKGWKPVVAPELEFYLVDMNKDPDLPLRPPVGRTGRPE
TGRQSYSIEAVNEFDPLFEDIYEYCETQGLDIDTLIHEVGAAQMEINFMHGDALSLADQVFLFKRTVREAALRHNMYATF
MAKPMEGEPGSAMHVHQSIVDEETGRNLFTSPETGGATSLFYNYLAGLQKYTPALMPIFAPYINSYRRLSRFMAAPINVQ
WGYDNRTVGFRIPHSGPAARRIENRIPGVDCNPYLALAATLAAGYLGMTQRLEPTEPLVSDGYDLPYQLPRNLEEGLTLM
AACEPLGEILGHKFLKAYFALKETEYEAFFRVISSWERRHLLLHV
>Mature_445_residues
MQDMEDFLRQHRITEVEAIIPDMAGIARGKITPRNKFTSGESMRLPQAVMVQTVTGEYPEDGSLTGVTDPDMVCVPDAST
IRLIPWASDPTAQVIHDCVHFDGSPVEISPRYVLRRVLDLYKAKGWKPVVAPELEFYLVDMNKDPDLPLRPPVGRTGRPE
TGRQSYSIEAVNEFDPLFEDIYEYCETQGLDIDTLIHEVGAAQMEINFMHGDALSLADQVFLFKRTVREAALRHNMYATF
MAKPMEGEPGSAMHVHQSIVDEETGRNLFTSPETGGATSLFYNYLAGLQKYTPALMPIFAPYINSYRRLSRFMAAPINVQ
WGYDNRTVGFRIPHSGPAARRIENRIPGVDCNPYLALAATLAAGYLGMTQRLEPTEPLVSDGYDLPYQLPRNLEEGLTLM
AACEPLGEILGHKFLKAYFALKETEYEAFFRVISSWERRHLLLHV

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=449, Percent_Identity=27.3942093541203, Blast_Score=154, Evalue=2e-37,
Organism=Escherichia coli, GI87081870, Length=451, Percent_Identity=34.3680709534368, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1790301, Length=439, Percent_Identity=28.4738041002278, Blast_Score=147, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 50128; Mature: 50128

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDMEDFLRQHRITEVEAIIPDMAGIARGKITPRNKFTSGESMRLPQAVMVQTVTGEYPE
CCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCC
DGSLTGVTDPDMVCVPDASTIRLIPWASDPTAQVIHDCVHFDGSPVEISPRYVLRRVLDL
CCCCCCCCCCCEEEECCCCEEEEEECCCCCHHHHHHHHHHCCCCCEEECHHHHHHHHHHH
YKAKGWKPVVAPELEFYLVDMNKDPDLPLRPPVGRTGRPETGRQSYSIEAVNEFDPLFED
HHCCCCCCEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHH
IYEYCETQGLDIDTLIHEVGAAQMEINFMHGDALSLADQVFLFKRTVREAALRHNMYATF
HHHHHHHCCCCHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHE
MAKPMEGEPGSAMHVHQSIVDEETGRNLFTSPETGGATSLFYNYLAGLQKYTPALMPIFA
EECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHH
PYINSYRRLSRFMAAPINVQWGYDNRTVGFRIPHSGPAARRIENRIPGVDCNPYLALAAT
HHHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHH
LAAGYLGMTQRLEPTEPLVSDGYDLPYQLPRNLEEGLTLMAACEPLGEILGHKFLKAYFA
HHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKETEYEAFFRVISSWERRHLLLHV
HHHHHHHHHHHHHHHHHHCEEEEEC
>Mature Secondary Structure
MQDMEDFLRQHRITEVEAIIPDMAGIARGKITPRNKFTSGESMRLPQAVMVQTVTGEYPE
CCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCC
DGSLTGVTDPDMVCVPDASTIRLIPWASDPTAQVIHDCVHFDGSPVEISPRYVLRRVLDL
CCCCCCCCCCCEEEECCCCEEEEEECCCCCHHHHHHHHHHCCCCCEEECHHHHHHHHHHH
YKAKGWKPVVAPELEFYLVDMNKDPDLPLRPPVGRTGRPETGRQSYSIEAVNEFDPLFED
HHCCCCCCEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHH
IYEYCETQGLDIDTLIHEVGAAQMEINFMHGDALSLADQVFLFKRTVREAALRHNMYATF
HHHHHHHCCCCHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHE
MAKPMEGEPGSAMHVHQSIVDEETGRNLFTSPETGGATSLFYNYLAGLQKYTPALMPIFA
EECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHH
PYINSYRRLSRFMAAPINVQWGYDNRTVGFRIPHSGPAARRIENRIPGVDCNPYLALAAT
HHHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHH
LAAGYLGMTQRLEPTEPLVSDGYDLPYQLPRNLEEGLTLMAACEPLGEILGHKFLKAYFA
HHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKETEYEAFFRVISSWERRHLLLHV
HHHHHHHHHHHHHHHHHHCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]