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The map label for this gene is birA [H]

Identifier: 115353004

GI number: 115353004

Start: 3256908

End: 3257828

Strand: Direct

Name: birA [H]

Synonym: Bamb_2953

Alternate gene names: 115353004

Gene position: 3256908-3257828 (Clockwise)

Preceding gene: 115353003

Following gene: 115353005

Centisome position: 91.58

GC content: 74.7

Gene sequence:

>921_bases
ATGAACGCCCCCACCTCCTCCGACACGCCCGATACCGGTGACGCGCGCATCGCGCGCAACCGGCTCGAGGCGCACCTGGA
TGCCGCGCCGCGCGCGTGGCCGCTCGACATCGTCGCCGCCACCGGCTCGACCAACGCCGACGTCGCGACGCGCCTGAAGG
CGCTGCCGCGCACCGCGAGCGCGCTGCCCGCGCCGCTGGTGCGCGTCGCGTTCGAGCAGACGGCCGGCCGCGGTCGGCAA
GGCCGCCCGTGGTTCGCGCAACCCGGCAACGCGCTGCTGTGCTCGGTCGGCTGCATCGTGCCGCGCCCCGTCGACGCGCT
CGCCGGCCTGAGCATCGCGATCGGCGTCGCGCTCGCGGAAGGGCTCGCCGCGCTGCCGCTCGACGCCCGCTCGCGTGTCG
CCCTCAAATGGCCGAACGACCTGCTGCTGACGGCCACCGACGACGGTACGTCGCACATCGTCGGCAAGCTCGCGGGGATC
CTGATCGAAACCGTGTGGAGCACCGCCGACGCGACCGCCGTCGTGATCGGCTTCGGCATCAACGTGCGCGGCGCGGAAGC
CGTCGCCGCGCAGGTCGAAGCGCTGCGCGCGCGCGAAGCGACGCTCGCGAGCGGGCTGCCGCCGGCCGCGCTGTCGGCCG
CGTGCGCATCGGCCAACCTCACCGATACGCTCGCCGCCGCATTGAACGCGCTCACGCCCGCCCTCGCGCAGTTCGGCACC
GACGGGCTCGCGCCGTTCCTGCCGCGCTGGCACGCGCTGCACGCGTACGCCGGACGCGAAGTCGTGCTGCTCGAACGGGG
CGTCGAACGCGCGCGCGGCGTCGCGACGGGCATCGACGCGACCGGCCAGTTGCTGCTCGACACGCCCGACGGCGTGCTGG
CGATCGCGGCCGGCGACGTATCGCTGCGCGAAGCGCAATGA

Upstream 100 bases:

>100_bases
ACGGACCGCTGCGCGCGGCGCGGCAAACGCGGCACCGTCGAACGCCGCGGCCAAACCGCCGCCCGGCACGCACCGCCCCC
CTGTCGCTACAATATGCGCC

Downstream 100 bases:

>100_bases
ACGACCCGCATCTGCTGATCGACGCCGGCAACAGCCGGATCAAGTGGGCGCTCGCCGATGCGCGGCGCACGCTCGTCGAC
ACGGGCGCGTTCGGCCATAC

Product: biotin--protein ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MNAPTSSDTPDTGDARIARNRLEAHLDAAPRAWPLDIVAATGSTNADVATRLKALPRTASALPAPLVRVAFEQTAGRGRQ
GRPWFAQPGNALLCSVGCIVPRPVDALAGLSIAIGVALAEGLAALPLDARSRVALKWPNDLLLTATDDGTSHIVGKLAGI
LIETVWSTADATAVVIGFGINVRGAEAVAAQVEALRAREATLASGLPPAALSAACASANLTDTLAAALNALTPALAQFGT
DGLAPFLPRWHALHAYAGREVVLLERGVERARGVATGIDATGQLLLDTPDGVLAIAAGDVSLREAQ

Sequences:

>Translated_306_residues
MNAPTSSDTPDTGDARIARNRLEAHLDAAPRAWPLDIVAATGSTNADVATRLKALPRTASALPAPLVRVAFEQTAGRGRQ
GRPWFAQPGNALLCSVGCIVPRPVDALAGLSIAIGVALAEGLAALPLDARSRVALKWPNDLLLTATDDGTSHIVGKLAGI
LIETVWSTADATAVVIGFGINVRGAEAVAAQVEALRAREATLASGLPPAALSAACASANLTDTLAAALNALTPALAQFGT
DGLAPFLPRWHALHAYAGREVVLLERGVERARGVATGIDATGQLLLDTPDGVLAIAAGDVSLREAQ
>Mature_306_residues
MNAPTSSDTPDTGDARIARNRLEAHLDAAPRAWPLDIVAATGSTNADVATRLKALPRTASALPAPLVRVAFEQTAGRGRQ
GRPWFAQPGNALLCSVGCIVPRPVDALAGLSIAIGVALAEGLAALPLDARSRVALKWPNDLLLTATDDGTSHIVGKLAGI
LIETVWSTADATAVVIGFGINVRGAEAVAAQVEALRAREATLASGLPPAALSAACASANLTDTLAAALNALTPALAQFGT
DGLAPFLPRWHALHAYAGREVVLLERGVERARGVATGIDATGQLLLDTPDGVLAIAAGDVSLREAQ

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=241, Percent_Identity=37.7593360995851, Blast_Score=115, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004408
- InterPro:   IPR004409
- InterPro:   IPR003142
- InterPro:   IPR004143
- InterPro:   IPR013196
- InterPro:   IPR008988
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02237 BPL_C; PF03099 BPL_LipA_LipB; PF08279 HTH_11 [H]

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 31259; Mature: 31259

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPTSSDTPDTGDARIARNRLEAHLDAAPRAWPLDIVAATGSTNADVATRLKALPRTAS
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCHH
ALPAPLVRVAFEQTAGRGRQGRPWFAQPGNALLCSVGCIVPRPVDALAGLSIAIGVALAE
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
GLAALPLDARSRVALKWPNDLLLTATDDGTSHIVGKLAGILIETVWSTADATAVVIGFGI
CHHCCCCCCCCEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECC
NVRGAEAVAAQVEALRAREATLASGLPPAALSAACASANLTDTLAAALNALTPALAQFGT
CCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
DGLAPFLPRWHALHAYAGREVVLLERGVERARGVATGIDATGQLLLDTPDGVLAIAAGDV
CCCCHHHHHHHHHHHHCCCEEEEEHHHHHHHCCEECCCCCCCEEEEECCCCEEEEEECCE
SLREAQ
EECCCC
>Mature Secondary Structure
MNAPTSSDTPDTGDARIARNRLEAHLDAAPRAWPLDIVAATGSTNADVATRLKALPRTAS
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCHH
ALPAPLVRVAFEQTAGRGRQGRPWFAQPGNALLCSVGCIVPRPVDALAGLSIAIGVALAE
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
GLAALPLDARSRVALKWPNDLLLTATDDGTSHIVGKLAGILIETVWSTADATAVVIGFGI
CHHCCCCCCCCEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECC
NVRGAEAVAAQVEALRAREATLASGLPPAALSAACASANLTDTLAAALNALTPALAQFGT
CCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
DGLAPFLPRWHALHAYAGREVVLLERGVERARGVATGIDATGQLLLDTPDGVLAIAAGDV
CCCCHHHHHHHHHHHHCCCEEEEEHHHHHHHCCEECCCCCCCEEEEECCCCEEEEEECCE
SLREAQ
EECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3899863; 3536662; 8265357; 9278503; 1311302; 1328157; 1409631; 11353844 [H]