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The map label for this gene is gcvA [H]

Identifier: 115352991

GI number: 115352991

Start: 3243432

End: 3244418

Strand: Direct

Name: gcvA [H]

Synonym: Bamb_2940

Alternate gene names: 115352991

Gene position: 3243432-3244418 (Clockwise)

Preceding gene: 115352987

Following gene: 115353002

Centisome position: 91.2

GC content: 67.27

Gene sequence:

>987_bases
ATGGACCTCCGCCAGCTCCCTGCGCTGAACGCCATTCGGGCGTTCGAAGCCGCTGCACGTCACGAGAATTTCTCGCGCGC
CGCCGACGAACTCTTCGTCACGCACGGCGCGGTCAGCCATCAGGTGCGCGCGCTCGAGGAGGAGCTGGGCGTGCAATTGT
TCGCGCGCAACGGCAAGCGGCTGTGCCTGACCGGCGCCGGCATGCGCTACGCGCAGCAGGTGCGCACCGCGCTGATGATG
CTGGCCGACGCGACGCGCGAGGTGCGGGCAAGCGACCGCGACCGGCGGCTGGTGGTGTCGATGCTGTCGTCGTTCGCCGC
GCGCTTCGTCACGCCGCGCATCGGCACGTTCATCGAGCGGCATCCGGAAATCGACGTCGAACTGCAGTCAACCAACAATC
TGACCGATTTCGCGCGCGACGACGTCGACCTCGCGATCCGCTTCGGCTTCGGCAACTATCCGGGGCTGCACGTCGAGCCG
CTGTTCGACGAAGTGTTCTTTCCGGCGTGCGCGCCGACGTTGAACGGCGGCAAGCTGCCGCAGACGCCCGCCGATCTCGT
GCACTACAACCTGCTGCGCTCGGACGACGAGCTGTGGCGGCCGTGGTTCGACGCGGCCGGGCTCGACACGCTGACCGAGC
CGAAACGCGGGATTCTGTATCAGGATTCGTCGAACCTGCTGCTGGCCGCGATCGAAGGCCAGGGCATCGCGCTGGTGCGG
CGTTCGCTGGCGGTGCACGACCTGCTCGACGGGCGCCTCGTGCGCCTGTTCGACATCGACGGGGCGAGCCCGTGGCACTA
TTTTTTCGTGTGTCCGCCGCCGCTGCTGAATGCGCCGCGCGTGCAGGCATTCAGGGCGTGGATGTTCGAGGAAGTCGCGC
AATTCAAGCTGCTGTGCGACGAACTGGATGCGCGGCGCGCGGCGGGGAAGACCCCCGCCGAGTGCGCGCAGGAAGGCGGA
TGGAAGGGACTTAGAGCACAACCTTGA

Upstream 100 bases:

>100_bases
TCGACTCCGCCAATAAGTGCTTCGATGGCTACAATCGTAGTCACTTAAAGGCGCCCGACCAAACGACCAATTTTCACGTC
GATGTGAGGAAAATTAGCAA

Downstream 100 bases:

>100_bases
CCATAGGATGGCCGGTCAAGGCGCGGTAGATGTTATCGAGCTGTTCCTGGCTCGTCGCGCGAACGGTGATCGTGAGGCCG
GTGTAATTACCGCCGCTCGA

Product: DNA-binding transcriptional activator GcvA

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MDLRQLPALNAIRAFEAAARHENFSRAADELFVTHGAVSHQVRALEEELGVQLFARNGKRLCLTGAGMRYAQQVRTALMM
LADATREVRASDRDRRLVVSMLSSFAARFVTPRIGTFIERHPEIDVELQSTNNLTDFARDDVDLAIRFGFGNYPGLHVEP
LFDEVFFPACAPTLNGGKLPQTPADLVHYNLLRSDDELWRPWFDAAGLDTLTEPKRGILYQDSSNLLLAAIEGQGIALVR
RSLAVHDLLDGRLVRLFDIDGASPWHYFFVCPPPLLNAPRVQAFRAWMFEEVAQFKLLCDELDARRAAGKTPAECAQEGG
WKGLRAQP

Sequences:

>Translated_328_residues
MDLRQLPALNAIRAFEAAARHENFSRAADELFVTHGAVSHQVRALEEELGVQLFARNGKRLCLTGAGMRYAQQVRTALMM
LADATREVRASDRDRRLVVSMLSSFAARFVTPRIGTFIERHPEIDVELQSTNNLTDFARDDVDLAIRFGFGNYPGLHVEP
LFDEVFFPACAPTLNGGKLPQTPADLVHYNLLRSDDELWRPWFDAAGLDTLTEPKRGILYQDSSNLLLAAIEGQGIALVR
RSLAVHDLLDGRLVRLFDIDGASPWHYFFVCPPPLLNAPRVQAFRAWMFEEVAQFKLLCDELDARRAAGKTPAECAQEGG
WKGLRAQP
>Mature_328_residues
MDLRQLPALNAIRAFEAAARHENFSRAADELFVTHGAVSHQVRALEEELGVQLFARNGKRLCLTGAGMRYAQQVRTALMM
LADATREVRASDRDRRLVVSMLSSFAARFVTPRIGTFIERHPEIDVELQSTNNLTDFARDDVDLAIRFGFGNYPGLHVEP
LFDEVFFPACAPTLNGGKLPQTPADLVHYNLLRSDDELWRPWFDAAGLDTLTEPKRGILYQDSSNLLLAAIEGQGIALVR
RSLAVHDLLDGRLVRLFDIDGASPWHYFFVCPPPLLNAPRVQAFRAWMFEEVAQFKLLCDELDARRAAGKTPAECAQEGG
WKGLRAQP

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=292, Percent_Identity=39.3835616438356, Blast_Score=206, Evalue=1e-54,
Organism=Escherichia coli, GI1788706, Length=293, Percent_Identity=29.3515358361775, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1786448, Length=298, Percent_Identity=31.5436241610738, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI145693193, Length=287, Percent_Identity=30.3135888501742, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1786401, Length=248, Percent_Identity=30.241935483871, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI157672245, Length=158, Percent_Identity=36.7088607594937, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI145693105, Length=167, Percent_Identity=28.1437125748503, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787128, Length=139, Percent_Identity=28.0575539568345, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87081978, Length=157, Percent_Identity=28.6624203821656, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787589, Length=143, Percent_Identity=28.6713286713287, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36729; Mature: 36729

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLRQLPALNAIRAFEAAARHENFSRAADELFVTHGAVSHQVRALEEELGVQLFARNGKR
CCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCEEEEECCCCE
LCLTGAGMRYAQQVRTALMMLADATREVRASDRDRRLVVSMLSSFAARFVTPRIGTFIER
EEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHH
HPEIDVELQSTNNLTDFARDDVDLAIRFGFGNYPGLHVEPLFDEVFFPACAPTLNGGKLP
CCCCEEEECCCCCHHHHHCCCEEEEEEECCCCCCCCEECHHHHHHHHHHCCCCCCCCCCC
QTPADLVHYNLLRSDDELWRPWFDAAGLDTLTEPKRGILYQDSSNLLLAAIEGQGIALVR
CCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCEEEECCCCEEEEEECCCCHHHHH
RSLAVHDLLDGRLVRLFDIDGASPWHYFFVCPPPLLNAPRVQAFRAWMFEEVAQFKLLCD
HHHHHHHHHCCCEEEEEECCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ELDARRAAGKTPAECAQEGGWKGLRAQP
HHHHHHHCCCCHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MDLRQLPALNAIRAFEAAARHENFSRAADELFVTHGAVSHQVRALEEELGVQLFARNGKR
CCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCEEEEECCCCE
LCLTGAGMRYAQQVRTALMMLADATREVRASDRDRRLVVSMLSSFAARFVTPRIGTFIER
EEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHH
HPEIDVELQSTNNLTDFARDDVDLAIRFGFGNYPGLHVEPLFDEVFFPACAPTLNGGKLP
CCCCEEEECCCCCHHHHHCCCEEEEEEECCCCCCCCEECHHHHHHHHHHCCCCCCCCCCC
QTPADLVHYNLLRSDDELWRPWFDAAGLDTLTEPKRGILYQDSSNLLLAAIEGQGIALVR
CCHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCEEEECCCCEEEEEECCCCHHHHH
RSLAVHDLLDGRLVRLFDIDGASPWHYFFVCPPPLLNAPRVQAFRAWMFEEVAQFKLLCD
HHHHHHHHHCCCEEEEEECCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ELDARRAAGKTPAECAQEGGWKGLRAQP
HHHHHHHCCCCHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]